mmp8  (MedChemExpress)

Journal: Respiratory Research

Article Title: Decoding the ubiquitination-immunity axis in idiopathic pulmonary fibrosis: diagnostic insights and therapeutic implications

doi: 10.1186/s12931-026-03612-7

Figure Lengend Snippet: UBD knockdown in fibroblasts facilitates neutrophil migration. A - B Differential expression of CXCL2 ( A ) and CXCL3 ( B ) in fibroblasts from human IPF and control lungs in the scRNA-seq dataset GSE122960 . C - D RT-qPCR analysis showing increased expression of Cxcl2 ( C ) and Cxcl3 ( D ) in mouse PLFs following Ubd knockdown. E Schematic illustration of the cell-based transwell co-culture assay. PLFs were transfected with the indicated siRNAs for 48 h and then seeded in the lower chamber. After 12 h of culture, 50 nM N-formyl-Met-Leu-Phe (fMLP) was added to the lower chamber, and neutrophils were placed in the upper chamber. After 4 h, medium from the lower chamber containing migrated neutrophils was collected, transferred to a clean 24-well plate, and neutrophils were imaged and counted under an optical microscope. F Representative images and quantification of neutrophil migration toward control or Ubd -knockdown PLFs in the cell-based transwell assay. Scale bar = 50 μm. G Schematic illustration of the conditioned medium-based transwell migration assay. PLFs were transfected with the indicated siRNAs for 48 h, after which culture supernatants were collected and transferred to the lower chamber. 50 nM fMLP was added, and neutrophils were placed in the upper chamber. After 4 h, medium from the lower chamber was collected, transferred to a clean 24-well plate, and migrated neutrophils were imaged and counted. H Representative images and quantification of neutrophil migration induced by conditioned media from control or Ubd -knockdown PLFs. Scale bar = 50 μm. All PLFs were isolated from untreated wild-type mice unless otherwise indicated. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns: not significant

Article Snippet: To facilitate neutrophil migration, 50 nM N-formyl-Met-Leu-Phe (fMLP) (HY-P0224; MedChemExpress) was added to the lower chamber.

Techniques: Knockdown, Migration, Quantitative Proteomics, Control, Quantitative RT-PCR, Expressing, Co-culture Assay, Transfection, Microscopy, Transwell Assay, Transwell Migration Assay, Isolation

Journal: Journal of Translational Medicine

Article Title: Micheliolide suppresses epithelial-mesenchymal transition of lens epithelial cells via downregulating matrix metalloproteinase 8 to ameliorate posterior capsular opacification

doi: 10.1186/s12967-026-07890-z

Figure Lengend Snippet: Identification of Micheliolide, a potential MMP8 regulator. A , qRT-PCR analysis of MMP8 expression in capsular tissues 24 hours after ECLE surgery, combined with anterior chamber injection of saline or MCL, n = 3, N = 18. B , Western blot analysis and quantification of MMP8 protein levels in capsular tissues 24 hours after ECLE surgery with anterior chamber injection of saline or MCL, n = 4, N = 20. C , qRT-PCR analysis of MMP8 expression in LECs pretreated with or without 5 μmol/L MCL and stimulated with 10 ng/mL TGF-β2 for 48 hours, n = 6. D , Western blot and corresponding quantification of MMP8 protein expression in LECs pretreated with or without 5 μmol/L MCL followed by stimulation with 10 ng/mL TGF-β2 for 48 hours, n = 4. E , Molecular docking simulation of Micheliolide and MCL, with the interacting MMP8 amino acids represented by blue sticks. F , CETSA was used to assess the thermal instability of the interaction between MMP8 and MCL over the temperature range of 58 °C to 78 °C, n = 4

Article Snippet: To explore the role of Matrix Metalloproteinase 8 (MMP8) in the effect of MCL on EMT, a MMP8 inhibitor (Cat. HY-N1454, MedChemExpress, State of New Jersey, USA), referred to as M8I, was used in the present study.

Techniques: Quantitative RT-PCR, Expressing, Injection, Saline, Western Blot

Journal: Journal of Translational Medicine

Article Title: Micheliolide suppresses epithelial-mesenchymal transition of lens epithelial cells via downregulating matrix metalloproteinase 8 to ameliorate posterior capsular opacification

doi: 10.1186/s12967-026-07890-z

Figure Lengend Snippet: MCL inhibits EMT by regulating MMP8 expression. A , Viability of LECs treated with a gradient concentration of M8I, n = 8. B , Western blot analysis of EMT-related markers in LECs induced by TGF-β2 and treated with MCL alone or in combination with M8I, n = 4. C , Quantitative analysis of the Western blot results shown in ( B ), normalized to GAPDH. D , Coimmunofluorescence staining for αSMA (red) and MMP8 (green) in LECs induced by TGFβ2 and treated with MCL alone or in combination with M8I, n = 3, scale bar = 100um. E , Quantification of immunofluorescence intensity

Article Snippet: To explore the role of Matrix Metalloproteinase 8 (MMP8) in the effect of MCL on EMT, a MMP8 inhibitor (Cat. HY-N1454, MedChemExpress, State of New Jersey, USA), referred to as M8I, was used in the present study.

Techniques: Expressing, Concentration Assay, Western Blot, Staining, Immunofluorescence

Journal: Journal of Translational Medicine

Article Title: Micheliolide suppresses epithelial-mesenchymal transition of lens epithelial cells via downregulating matrix metalloproteinase 8 to ameliorate posterior capsular opacification

doi: 10.1186/s12967-026-07890-z

Figure Lengend Snippet: MCL ameliorates the inflammatory response and suppresses the proliferation, migration, and EMT of LECs following cataract surgery via targeting MMP8. PCS, post-cataract surgery

Article Snippet: To explore the role of Matrix Metalloproteinase 8 (MMP8) in the effect of MCL on EMT, a MMP8 inhibitor (Cat. HY-N1454, MedChemExpress, State of New Jersey, USA), referred to as M8I, was used in the present study.

Techniques: Migration

Journal: Journal of Translational Medicine

Article Title: Micheliolide suppresses epithelial-mesenchymal transition of lens epithelial cells via downregulating matrix metalloproteinase 8 to ameliorate posterior capsular opacification

doi: 10.1186/s12967-026-07890-z

Figure Lengend Snippet: Identification of Micheliolide, a potential MMP8 regulator. A , qRT-PCR analysis of MMP8 expression in capsular tissues 24 hours after ECLE surgery, combined with anterior chamber injection of saline or MCL, n = 3, N = 18. B , Western blot analysis and quantification of MMP8 protein levels in capsular tissues 24 hours after ECLE surgery with anterior chamber injection of saline or MCL, n = 4, N = 20. C , qRT-PCR analysis of MMP8 expression in LECs pretreated with or without 5 μmol/L MCL and stimulated with 10 ng/mL TGF-β2 for 48 hours, n = 6. D , Western blot and corresponding quantification of MMP8 protein expression in LECs pretreated with or without 5 μmol/L MCL followed by stimulation with 10 ng/mL TGF-β2 for 48 hours, n = 4. E , Molecular docking simulation of Micheliolide and MCL, with the interacting MMP8 amino acids represented by blue sticks. F , CETSA was used to assess the thermal instability of the interaction between MMP8 and MCL over the temperature range of 58 °C to 78 °C, n = 4

Article Snippet: To explore the role of Matrix Metalloproteinase 8 (MMP8) in the effect of MCL on EMT, a MMP8 inhibitor (Cat. HY-N1454, MedChemExpress, State of New Jersey, USA), referred to as M8I, was used in the present study.

Techniques: Quantitative RT-PCR, Expressing, Injection, Saline, Western Blot

Journal: Journal of Translational Medicine

Article Title: Micheliolide suppresses epithelial-mesenchymal transition of lens epithelial cells via downregulating matrix metalloproteinase 8 to ameliorate posterior capsular opacification

doi: 10.1186/s12967-026-07890-z

Figure Lengend Snippet: MCL inhibits EMT by regulating MMP8 expression. A , Viability of LECs treated with a gradient concentration of M8I, n = 8. B , Western blot analysis of EMT-related markers in LECs induced by TGF-β2 and treated with MCL alone or in combination with M8I, n = 4. C , Quantitative analysis of the Western blot results shown in ( B ), normalized to GAPDH. D , Coimmunofluorescence staining for αSMA (red) and MMP8 (green) in LECs induced by TGFβ2 and treated with MCL alone or in combination with M8I, n = 3, scale bar = 100um. E , Quantification of immunofluorescence intensity

Article Snippet: To explore the role of Matrix Metalloproteinase 8 (MMP8) in the effect of MCL on EMT, a MMP8 inhibitor (Cat. HY-N1454, MedChemExpress, State of New Jersey, USA), referred to as M8I, was used in the present study.

Techniques: Expressing, Concentration Assay, Western Blot, Staining, Immunofluorescence

Journal: Journal of Translational Medicine

Article Title: Micheliolide suppresses epithelial-mesenchymal transition of lens epithelial cells via downregulating matrix metalloproteinase 8 to ameliorate posterior capsular opacification

doi: 10.1186/s12967-026-07890-z

Figure Lengend Snippet: MCL ameliorates the inflammatory response and suppresses the proliferation, migration, and EMT of LECs following cataract surgery via targeting MMP8. PCS, post-cataract surgery

Article Snippet: To explore the role of Matrix Metalloproteinase 8 (MMP8) in the effect of MCL on EMT, a MMP8 inhibitor (Cat. HY-N1454, MedChemExpress, State of New Jersey, USA), referred to as M8I, was used in the present study.

Techniques: Migration