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Proteintech anti mlph antibody
Anti Mlph Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti mlph antibody/product/Proteintech
Average 93 stars, based on 20 article reviews

anti mlph antibody - by Bioz Stars, 2026-03

93/100 stars

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Identification of the key gene MLPH related to lymph node metastasis in TNBC. a . Clustering dendrogram of the clinical traits. b . Calculation of the soft threshold by scale independence and mean connectivity. When the soft threshold was 3, the scale free topology fitting index R2 was 0.868. c and d . Clustering dendrogram of genes based on topological overlap. e . Heatmap of modules for gene-traits relationship. Red represents a positive correlation, and green represents a negative correlation. f . The correlation between yellow module membership and N staging was significant. The correlation coefficient was 0.37. g . Utilization of Cytoscape to visualize the relationship between genes in the yellow module. ten key genes were selected from the network using the Maximal Clique Centrality algorithm

Journal: Journal of Translational Medicine

Article Title: Melanophilin inhibit the growth and lymph node metastasis of triple negative breast cancer via the NONO-SPHK1-S1P axis

doi: 10.1186/s12967-025-06240-9

Figure Lengend Snippet: Identification of the key gene MLPH related to lymph node metastasis in TNBC. a . Clustering dendrogram of the clinical traits. b . Calculation of the soft threshold by scale independence and mean connectivity. When the soft threshold was 3, the scale free topology fitting index R2 was 0.868. c and d . Clustering dendrogram of genes based on topological overlap. e . Heatmap of modules for gene-traits relationship. Red represents a positive correlation, and green represents a negative correlation. f . The correlation between yellow module membership and N staging was significant. The correlation coefficient was 0.37. g . Utilization of Cytoscape to visualize the relationship between genes in the yellow module. ten key genes were selected from the network using the Maximal Clique Centrality algorithm

Article Snippet: MLPH-Lentivirus and MLPH RNAi-Lentivirus were purchased from GeneChem Company (Shanghai, China).

Techniques:

MLPH is downregulated and negatively correlated with lymph node metastasis in TNBC. a . RFS curves for the key gene MLPH of patients with/ without lymph node metastasis in the GEO databases. b . Predict the expression of MLPH in different subtypes breast cancer and adjacent cancer in TCGA database. c . Predict the protein expression of MLPH in different subtypes breast cancer and normal tissues in CPTAC database. d and e . MLPH expression levels in 80 different subtypes clinical breast tissues were determined by Western blotting with the indicated antibodies. MLPH protein levels were quantified by normalizing to the intensity of the Actin band. Error bars represent the mean ± SEM. * p < 0.05, *** p < 0.001. f . Representative images of IHC staining. The boxed areas in the top images are magnified in the bottom images. Original magnification, 100×. g . MLPH expression levels in breast cancer tissues. The intensity values in (a) are expressed as the H-score. The relative staining across all samples was shown in the boxplot (t test, * p < 0.05, ** p < 0.01). h . MLPH expression levels in different subtypes breast cancer tissues (with and without metastasis). The intensity values in ( a ) are expressed as the H-score. The relative staining across all samples was shown in the boxplot (t-test, * p < 0.05). i . Kaplan-Meier plot of OS or disease-free survival (DFS) of different subtypes breast cancer patients stratified by MLPH expression levels

Journal: Journal of Translational Medicine

Article Title: Melanophilin inhibit the growth and lymph node metastasis of triple negative breast cancer via the NONO-SPHK1-S1P axis

doi: 10.1186/s12967-025-06240-9

Figure Lengend Snippet: MLPH is downregulated and negatively correlated with lymph node metastasis in TNBC. a . RFS curves for the key gene MLPH of patients with/ without lymph node metastasis in the GEO databases. b . Predict the expression of MLPH in different subtypes breast cancer and adjacent cancer in TCGA database. c . Predict the protein expression of MLPH in different subtypes breast cancer and normal tissues in CPTAC database. d and e . MLPH expression levels in 80 different subtypes clinical breast tissues were determined by Western blotting with the indicated antibodies. MLPH protein levels were quantified by normalizing to the intensity of the Actin band. Error bars represent the mean ± SEM. * p < 0.05, *** p < 0.001. f . Representative images of IHC staining. The boxed areas in the top images are magnified in the bottom images. Original magnification, 100×. g . MLPH expression levels in breast cancer tissues. The intensity values in (a) are expressed as the H-score. The relative staining across all samples was shown in the boxplot (t test, * p < 0.05, ** p < 0.01). h . MLPH expression levels in different subtypes breast cancer tissues (with and without metastasis). The intensity values in ( a ) are expressed as the H-score. The relative staining across all samples was shown in the boxplot (t-test, * p < 0.05). i . Kaplan-Meier plot of OS or disease-free survival (DFS) of different subtypes breast cancer patients stratified by MLPH expression levels

Article Snippet: MLPH-Lentivirus and MLPH RNAi-Lentivirus were purchased from GeneChem Company (Shanghai, China).

Techniques: Expressing, Western Blot, Immunohistochemistry, Staining

MLPH inhibits the proliferation, invasion, and migration of TNBC. a . The protein expression of MLPH protein was detected in cell lines by western blot. MLPH protein levels were quantified by normalizing to the intensity of the Actin band. Error bars represent the mean ± SEM. * p < 0.05, *** p < 0.001. b . Wound-healing analysis of different subtypes breast cancer cells. Results were representative of three independent experiments. **** p < 0.0001. c . Transwell analysis of different subtypes breast cancer cells. Results were representative of three independent experiments. **** p < 0.0001. d and e . CCK8 proliferation analysis of HS578T or MDA-MB-231 cells stable transfected with Flag or Flag-MLPH, the data were presented as a histogram of the mean and SEM of three independent experiments, **** p < 0.0001. f . Colony formation analysis of HS578T or MDA-MB-231 cells stable transfected with Flag or Flag-MLPH, representative pictures of the colonies were shown. The data were presented as a histogram of the mean and SEM of three independent experiments. **** p < 0.0001. g and h . Wound-healing analysis of MDA-MB-231( g ) or HS578T( h ) cells stable transfected with Flag or Flag-MLPH. Results were representative of three independent experiments. *** p < 0.001, **** p < 0.0001. i and j . Transwell analysis of MDA-MB-231( i ) or HS578T( j ) cells stable transfected with Flag or Flag-MLPH. Results were representative of three independent experiments. **** p < 0.0001

Journal: Journal of Translational Medicine

Article Title: Melanophilin inhibit the growth and lymph node metastasis of triple negative breast cancer via the NONO-SPHK1-S1P axis

doi: 10.1186/s12967-025-06240-9

Figure Lengend Snippet: MLPH inhibits the proliferation, invasion, and migration of TNBC. a . The protein expression of MLPH protein was detected in cell lines by western blot. MLPH protein levels were quantified by normalizing to the intensity of the Actin band. Error bars represent the mean ± SEM. * p < 0.05, *** p < 0.001. b . Wound-healing analysis of different subtypes breast cancer cells. Results were representative of three independent experiments. **** p < 0.0001. c . Transwell analysis of different subtypes breast cancer cells. Results were representative of three independent experiments. **** p < 0.0001. d and e . CCK8 proliferation analysis of HS578T or MDA-MB-231 cells stable transfected with Flag or Flag-MLPH, the data were presented as a histogram of the mean and SEM of three independent experiments, **** p < 0.0001. f . Colony formation analysis of HS578T or MDA-MB-231 cells stable transfected with Flag or Flag-MLPH, representative pictures of the colonies were shown. The data were presented as a histogram of the mean and SEM of three independent experiments. **** p < 0.0001. g and h . Wound-healing analysis of MDA-MB-231( g ) or HS578T( h ) cells stable transfected with Flag or Flag-MLPH. Results were representative of three independent experiments. *** p < 0.001, **** p < 0.0001. i and j . Transwell analysis of MDA-MB-231( i ) or HS578T( j ) cells stable transfected with Flag or Flag-MLPH. Results were representative of three independent experiments. **** p < 0.0001

Article Snippet: MLPH-Lentivirus and MLPH RNAi-Lentivirus were purchased from GeneChem Company (Shanghai, China).

Techniques: Migration, Expressing, Western Blot, Transfection

MLPH prevents the binding of NONO to the SPHK1 pre-RNA to inhibit SPHK1 mRNA splicing in TNBC. a . Heatmap analysis of expression differential proteins regulated by MLPH in MDA-MB-231 cells. b . qRT-PCR analysis of the expression levels of differential gene in Flag-MLPH overexpressing cells. Levels of all mRNAs were normalized with GAPDH. The data were presented as a histogram of the mean SEM of three independent experiments. Error bars represent mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, t-test. c . Predict the expression of SPHK1 in different subtypes breast cancer and adjacent cancer in TCGA database. d . RFS curves for the key gene SPHK1 of patients with/ without lymph node metastasis in the GEO databases. e . SK-BR-3, MDA-MB-231 and HS578T cells transfected with Flag or Flag -MLPH; MCF7 cells knock out MLPH, the protein expression was determined by western blotting with the indicated antibodies. f . SK-BR-3, MDA-MB-231 and HS578T cells transfected with Flag or Flag -MLPH, qRT-PCR analysis of the expression levels of MLPH, pre-SPHK1, SPHK1. Levels of all mRNAs were normalized with GAPDH. The data were presented as a histogram of the mean SEM of three independent experiments. Error bars represent mean ± SD. * p < 0.05, *** p < 0.001, t-test. g and h . IP and IB analysis of the interaction of MLPH and NONO in MDA-MB-231 cells. i . Separation of cytoplasmic protein and nuclear protein with nucleoplasm separation kit, lysates were subjected to immunoprecipitation and western blot with indicated antibodies. j . RIP, qRT-PCR and IB analysis of the interaction of NONO and RNA-SPHK1in MDA-MB-231 cells. k and l . MDA-MB-231 and HS578T cells transfected with Flag or Flag -MLPH RIP, qRT-PCR and IB analysis of the interaction of NONO and RNA-SPHK1

Journal: Journal of Translational Medicine

Article Title: Melanophilin inhibit the growth and lymph node metastasis of triple negative breast cancer via the NONO-SPHK1-S1P axis

doi: 10.1186/s12967-025-06240-9

Figure Lengend Snippet: MLPH prevents the binding of NONO to the SPHK1 pre-RNA to inhibit SPHK1 mRNA splicing in TNBC. a . Heatmap analysis of expression differential proteins regulated by MLPH in MDA-MB-231 cells. b . qRT-PCR analysis of the expression levels of differential gene in Flag-MLPH overexpressing cells. Levels of all mRNAs were normalized with GAPDH. The data were presented as a histogram of the mean SEM of three independent experiments. Error bars represent mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, t-test. c . Predict the expression of SPHK1 in different subtypes breast cancer and adjacent cancer in TCGA database. d . RFS curves for the key gene SPHK1 of patients with/ without lymph node metastasis in the GEO databases. e . SK-BR-3, MDA-MB-231 and HS578T cells transfected with Flag or Flag -MLPH; MCF7 cells knock out MLPH, the protein expression was determined by western blotting with the indicated antibodies. f . SK-BR-3, MDA-MB-231 and HS578T cells transfected with Flag or Flag -MLPH, qRT-PCR analysis of the expression levels of MLPH, pre-SPHK1, SPHK1. Levels of all mRNAs were normalized with GAPDH. The data were presented as a histogram of the mean SEM of three independent experiments. Error bars represent mean ± SD. * p < 0.05, *** p < 0.001, t-test. g and h . IP and IB analysis of the interaction of MLPH and NONO in MDA-MB-231 cells. i . Separation of cytoplasmic protein and nuclear protein with nucleoplasm separation kit, lysates were subjected to immunoprecipitation and western blot with indicated antibodies. j . RIP, qRT-PCR and IB analysis of the interaction of NONO and RNA-SPHK1in MDA-MB-231 cells. k and l . MDA-MB-231 and HS578T cells transfected with Flag or Flag -MLPH RIP, qRT-PCR and IB analysis of the interaction of NONO and RNA-SPHK1

Article Snippet: MLPH-Lentivirus and MLPH RNAi-Lentivirus were purchased from GeneChem Company (Shanghai, China).

Techniques: Binding Assay, Expressing, Quantitative RT-PCR, Transfection, Knock-Out, Western Blot, Immunoprecipitation

MLPH inhibits cell proliferation, invasion, migration, and adhesion in TNBC through the SPHK1-S1P axis. a . MLPH and SPHK1 expression levels in 48 different subtypes clinical breast tissues were determined by Western blotting with the indicated antibodies. b . Relative S1P levels of tumor tissues (T). unpaired Student’s t-test, n = 48, * p < 0.05. c . SK-BR-3, MDA-MB-231 and HS578T cells transfected with Flag or Flag -MLPH, and detected the content of intracellular and extracellular S1P. d . CCK8 proliferation analysis of HS578T or MDA-MB-231 cells stable transfected with Flag or Flag-MLPH and added S1P (2 µg/ml), the data were presented as a histogram of the mean and SEM of three independent experiments, **** p < 0.0001. e . Colony formation analysis of HS578T or MDA-MB-231 cells stable transfected with Flag or Flag-MLPH and added S1P (2 µg/ml), representative pictures of the colonies were shown. The data were presented as a histogram of the mean and SEM of three independent experiments. **** p < 0.0001. f and g . Wound-healing analysis of MDA-MB-231(g) or HS578T(h) cells stable transfected with Flag or Flag-MLPH and added S1P (2 µg/ml). Results were representative of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001. h and i . Transwell analysis of MDA-MB-231( i ) or HS578T( j ) cells stable transfected with Flag or Flag-MLPH and added S1P (2 µg/ml). Results were representative of three independent experiments. ** p < 0.01, *** p < 0.001, **** p < 0.0001. j . GO analysis of MLPH enriched pathways. k . GSEA analysis of MLPH enriched pathways in TCGA databases. l . Cell adhesion analysis of HS578T or MDA-MB-231 cells stable transfected with Flag or Flag-MLPH and added S1P (2 µg/ml), the data were presented as a histogram of the mean and SEM of three independent experiments, * p < 0.05, ** p < 0.01, *** p < 0.001

Journal: Journal of Translational Medicine

Article Title: Melanophilin inhibit the growth and lymph node metastasis of triple negative breast cancer via the NONO-SPHK1-S1P axis

doi: 10.1186/s12967-025-06240-9

Figure Lengend Snippet: MLPH inhibits cell proliferation, invasion, migration, and adhesion in TNBC through the SPHK1-S1P axis. a . MLPH and SPHK1 expression levels in 48 different subtypes clinical breast tissues were determined by Western blotting with the indicated antibodies. b . Relative S1P levels of tumor tissues (T). unpaired Student’s t-test, n = 48, * p < 0.05. c . SK-BR-3, MDA-MB-231 and HS578T cells transfected with Flag or Flag -MLPH, and detected the content of intracellular and extracellular S1P. d . CCK8 proliferation analysis of HS578T or MDA-MB-231 cells stable transfected with Flag or Flag-MLPH and added S1P (2 µg/ml), the data were presented as a histogram of the mean and SEM of three independent experiments, **** p < 0.0001. e . Colony formation analysis of HS578T or MDA-MB-231 cells stable transfected with Flag or Flag-MLPH and added S1P (2 µg/ml), representative pictures of the colonies were shown. The data were presented as a histogram of the mean and SEM of three independent experiments. **** p < 0.0001. f and g . Wound-healing analysis of MDA-MB-231(g) or HS578T(h) cells stable transfected with Flag or Flag-MLPH and added S1P (2 µg/ml). Results were representative of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001. h and i . Transwell analysis of MDA-MB-231( i ) or HS578T( j ) cells stable transfected with Flag or Flag-MLPH and added S1P (2 µg/ml). Results were representative of three independent experiments. ** p < 0.01, *** p < 0.001, **** p < 0.0001. j . GO analysis of MLPH enriched pathways. k . GSEA analysis of MLPH enriched pathways in TCGA databases. l . Cell adhesion analysis of HS578T or MDA-MB-231 cells stable transfected with Flag or Flag-MLPH and added S1P (2 µg/ml), the data were presented as a histogram of the mean and SEM of three independent experiments, * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: MLPH-Lentivirus and MLPH RNAi-Lentivirus were purchased from GeneChem Company (Shanghai, China).

Techniques: Migration, Expressing, Western Blot, Transfection

MLPH inhibits proliferation and lymph node metastasis in TNBC in vivo. a . Representative mice with subcutaneously injected 4T-1 stably transfected cells. b . Representative tumor from Flag and Flag-MLPH 4T1 cells. c . Quantitation of tumor weights was presented as a histogram (5 mice per group. **** p < 0.0001). d . Tumor diameter was measured at the indicated time points, and tumor volume was calculated. The results were presented as the mean and SEM of 5 mice per group per time point, **** p < 0.0001. e . Representative images of IHC staining showing MLPH and SPHK1 protein expression in each group mice’s tumors. f . Pearson correlation analysis of PAK5 and HMGCS2 protein expression levels, **** p < 0.0001. g . Representative mice with subcutaneously injected 4T-1 stably transfected cells. h and i . Comparison of the gross appearances of metastatic pLNs and various metastasis parameters between Flag, Flag-MLPH and Flag-MLPH + S1P groups ( n = 5 samples for each group), * p < 0.05, Error bars are presented as mean ± SEM. j . Representative images of HE and IHC staining showing metastatic pLNs and highly magnified tumor margins. The boxed areas in the top images are magnified in the bottom images. Original magnification, 100×. k . Comparison of transfer regions in transferable pLNs. **** p < 0.0001, Error bars are presented as mean ± SEM

Journal: Journal of Translational Medicine

Article Title: Melanophilin inhibit the growth and lymph node metastasis of triple negative breast cancer via the NONO-SPHK1-S1P axis

doi: 10.1186/s12967-025-06240-9

Figure Lengend Snippet: MLPH inhibits proliferation and lymph node metastasis in TNBC in vivo. a . Representative mice with subcutaneously injected 4T-1 stably transfected cells. b . Representative tumor from Flag and Flag-MLPH 4T1 cells. c . Quantitation of tumor weights was presented as a histogram (5 mice per group. **** p < 0.0001). d . Tumor diameter was measured at the indicated time points, and tumor volume was calculated. The results were presented as the mean and SEM of 5 mice per group per time point, **** p < 0.0001. e . Representative images of IHC staining showing MLPH and SPHK1 protein expression in each group mice’s tumors. f . Pearson correlation analysis of PAK5 and HMGCS2 protein expression levels, **** p < 0.0001. g . Representative mice with subcutaneously injected 4T-1 stably transfected cells. h and i . Comparison of the gross appearances of metastatic pLNs and various metastasis parameters between Flag, Flag-MLPH and Flag-MLPH + S1P groups ( n = 5 samples for each group), * p < 0.05, Error bars are presented as mean ± SEM. j . Representative images of HE and IHC staining showing metastatic pLNs and highly magnified tumor margins. The boxed areas in the top images are magnified in the bottom images. Original magnification, 100×. k . Comparison of transfer regions in transferable pLNs. **** p < 0.0001, Error bars are presented as mean ± SEM

Article Snippet: MLPH-Lentivirus and MLPH RNAi-Lentivirus were purchased from GeneChem Company (Shanghai, China).

Techniques: In Vivo, Injection, Stable Transfection, Transfection, Quantitation Assay, Immunohistochemistry, Expressing, Comparison

The mechanisms of MLPH in controlling proliferation and lymph node metastasis in TNBC. In TNBC cells, MLPH is lower expression than other subtypes of breast cancer cells. NONO is identified as an essential factor involved in SPHK1 mRNA splicing. Ectopic overexpression of MLPH interacts with NONO to inhibit mRNA splicing of SPHK1, which reduces the content of S1P, thereby inhibiting growth and lymph node metastasis in TNBC

Journal: Journal of Translational Medicine

Article Title: Melanophilin inhibit the growth and lymph node metastasis of triple negative breast cancer via the NONO-SPHK1-S1P axis

doi: 10.1186/s12967-025-06240-9

Figure Lengend Snippet: The mechanisms of MLPH in controlling proliferation and lymph node metastasis in TNBC. In TNBC cells, MLPH is lower expression than other subtypes of breast cancer cells. NONO is identified as an essential factor involved in SPHK1 mRNA splicing. Ectopic overexpression of MLPH interacts with NONO to inhibit mRNA splicing of SPHK1, which reduces the content of S1P, thereby inhibiting growth and lymph node metastasis in TNBC

Article Snippet: MLPH-Lentivirus and MLPH RNAi-Lentivirus were purchased from GeneChem Company (Shanghai, China).

Techniques: Expressing, Over Expression

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