pleural mesothelial cell pmc line met 5a cells  (ATCC)

Journal: bioRxiv

Article Title: Cancer-Associated Mesothelial Cells Drive Immune Escape and Therapy Resistance in Ovarian Cancer

doi: 10.64898/2026.01.07.698232

Figure Lengend Snippet: A) Quantitative PCR (qPCR) analysis of Upk3b and Serpinb2 expression in immortalized mesothelial cells (iMC) stimulated with KPCA-conditioned media and Met5a stimulated with patient D HGSOC media (ptD) or Ov90 ovarian cancer cell line in a time-dependent manner (0-96hrs). B) Western blot analysis of UPK3B and SERPINB2 expression in iMC treated with KPCA-condioned culture medium at 0h, 24h, 48h, 72h and 96h (left panel) and Met5A stimulated with conditioned media from ptD/Ov90 cell-lines versus unstimulated controls at 72h (right panel). C) Immunofluorescence analysis of UPK3B and SERPINB2 in iMC and Met5A comparing conditions stimulated with either KPCA-conditioned media or ptD/Ov90 media versus unstimulated controls at 72 h. D) Immunofluorescence staining for ACTA2 and COL4A1 in both primary MCs and immortalized MCs (iMCs) after 96 hours of exposure to KPCA-conditioned media

Article Snippet: The Met5a cell line was purchased from ATCC and cultured in DMEM medium supplemented with 5% FBS.

Techniques: Real-time Polymerase Chain Reaction, Expressing, Western Blot, Immunofluorescence, Staining

Journal: Proteomes

Article Title: Proteomics and Bioinformatics Profiles of Human Mesothelial Cell Line MeT-5A

doi: 10.3390/proteomes14010002

Figure Lengend Snippet: Summary of cellular and conditioned medium proteomes of MeT-5A. ( a ) Venn diagram illustrating the total number of proteins identified by high-throughput LC-MS/MS from two distinct sample types: cellular lysate and conditioned cell culture medium. The numbers within each circle represent the total number of proteins uniquely identified in either the cellular lysate (blue circle) or the conditioned medium (green circle). The overlapping region indicates the number of proteins that were identified in both the cellular and conditioned medium samples. ( b ) Distribution of identified proteins (blue bars) relative to known total protein-coding genes (black bars) across human chromosomes. This bar plot illustrates the number of proteins identified in this study compared to the total number of protein-coding genes known for each individual chromosome. The x-axis represents the individual chromosomes, while the y-axis indicates the count of protein-coding genes or proteins. Blue bars denote the count of proteins identified in this study, while black bars represent the total number of protein-coding genes on each chromosome, according to Ensembl annotations.

Article Snippet: We cultured three technical replicates of MeT-5A cells (ATCC, Manassas, VA, USA) at 37 °C and 5% CO 2 in modified optimum medium.

Techniques: High Throughput Screening Assay, Liquid Chromatography with Mass Spectroscopy, Cell Culture

Journal: Proteomes

Article Title: Proteomics and Bioinformatics Profiles of Human Mesothelial Cell Line MeT-5A

doi: 10.3390/proteomes14010002

Figure Lengend Snippet: Dynamic range of cellular and conditioned medium proteomes of MeT-5A. ( a , b ) depict the wide dynamic range of proteins present in MeT5A cell lysate and cell conditioned medium, respectively, spanning seven orders of magnitude as shown by protein mean normalized abundance (PSM) on the Y-axis. The X-axis represents individual protein rank based on abundance. The abundance of each protein estimated based on the spectral count (defined by the total number of identified peptide spectra matched (PSM) to the protein of interest, including those redundantly identified) revealed the typical S-shaped distribution over the seven orders of dynamic range of MS signals. ( c , d ) show top ten most abundant proteins in the cell lysate and conditioned medium, respectively.

Article Snippet: We cultured three technical replicates of MeT-5A cells (ATCC, Manassas, VA, USA) at 37 °C and 5% CO 2 in modified optimum medium.

Techniques:

Journal: Proteomes

Article Title: Proteomics and Bioinformatics Profiles of Human Mesothelial Cell Line MeT-5A

doi: 10.3390/proteomes14010002

Figure Lengend Snippet: Summary of cellular and conditioned medium proteomes of MeT-5A. ( a ) Venn diagram illustrating the total number of proteins identified by high-throughput LC-MS/MS from two distinct sample types: cellular lysate and conditioned cell culture medium. The numbers within each circle represent the total number of proteins uniquely identified in either the cellular lysate (blue circle) or the conditioned medium (green circle). The overlapping region indicates the number of proteins that were identified in both the cellular and conditioned medium samples. ( b ) Distribution of identified proteins (blue bars) relative to known total protein-coding genes (black bars) across human chromosomes. This bar plot illustrates the number of proteins identified in this study compared to the total number of protein-coding genes known for each individual chromosome. The x-axis represents the individual chromosomes, while the y-axis indicates the count of protein-coding genes or proteins. Blue bars denote the count of proteins identified in this study, while black bars represent the total number of protein-coding genes on each chromosome, according to Ensembl annotations.

Article Snippet: The mesothelial cell line MeT-5A, obtained from the American Type Culture Collection (ATCC) and originally isolated from the pleural fluids of a non-cancerous individual, was used as the source of material for this study.

Techniques: High Throughput Screening Assay, Liquid Chromatography with Mass Spectroscopy, Cell Culture

Journal: Proteomes

Article Title: Proteomics and Bioinformatics Profiles of Human Mesothelial Cell Line MeT-5A

doi: 10.3390/proteomes14010002

Figure Lengend Snippet: Dynamic range of cellular and conditioned medium proteomes of MeT-5A. ( a , b ) depict the wide dynamic range of proteins present in MeT5A cell lysate and cell conditioned medium, respectively, spanning seven orders of magnitude as shown by protein mean normalized abundance (PSM) on the Y-axis. The X-axis represents individual protein rank based on abundance. The abundance of each protein estimated based on the spectral count (defined by the total number of identified peptide spectra matched (PSM) to the protein of interest, including those redundantly identified) revealed the typical S-shaped distribution over the seven orders of dynamic range of MS signals. ( c , d ) show top ten most abundant proteins in the cell lysate and conditioned medium, respectively.

Article Snippet: The mesothelial cell line MeT-5A, obtained from the American Type Culture Collection (ATCC) and originally isolated from the pleural fluids of a non-cancerous individual, was used as the source of material for this study.

Techniques: