acute senescence assay mouse embryonic fibroblasts  (ATCC)

Journal: Biogerontology

Article Title: Tumor-derived circulating DNA can induce senescence and SASP activation in mouse embryonic fibroblasts

doi: 10.1007/s10522-026-10400-9

Figure Lengend Snippet: Quantitative analysis of senescence associated gene expression in MEFs following 24 h cfDNA or ctDNA exposure. a p16, b p21, c p53

Article Snippet: Mouse embryonic fibroblasts (ATCC CRL-2991) were cultured at 37 °C in a humidified incubator with 5% CO 2 .

Techniques: Gene Expression

Journal: Biogerontology

Article Title: Tumor-derived circulating DNA can induce senescence and SASP activation in mouse embryonic fibroblasts

doi: 10.1007/s10522-026-10400-9

Figure Lengend Snippet: Quantitative analysis of SASP associated gene expression in MEFs following 24 h cfDNA or ctDNA exposure. a IL-1B, b IL-6

Article Snippet: Mouse embryonic fibroblasts (ATCC CRL-2991) were cultured at 37 °C in a humidified incubator with 5% CO 2 .

Techniques: Gene Expression

Journal: Biogerontology

Article Title: Tumor-derived circulating DNA can induce senescence and SASP activation in mouse embryonic fibroblasts

doi: 10.1007/s10522-026-10400-9

Figure Lengend Snippet: Senescence-associated β-galactosidase staining of MEFs following 24 h exposure to cfDNA or ctDNA. Images captured under brightfield microscopy using a 10 × objective. a NC, b Sen ( +) /PC, c ctDNA-LD, d ctDNA-HD, e cfDNA-LD, f cfDNA-HD

Article Snippet: Mouse embryonic fibroblasts (ATCC CRL-2991) were cultured at 37 °C in a humidified incubator with 5% CO 2 .

Techniques: Staining, Microscopy

Journal: Translational Pediatrics

Article Title: Adolescent high fructose consumption induces cardiac dysfunction in adulthood via elevated histone acetylation

doi: 10.21037/tp-2025-aw-728

Figure Lengend Snippet: Reduced Cpt1b expression and elevated histone acetylation mediated by Sirt1 downregulation contributed to fructose-induced cardiac hypertrophy and fibrosis respectively in adulthood. (A) The mRNA expression levels of Cpt1b , Khk and ChREBP in the heart (ND: n=12, HFD: n=12). (B) Histone acetylation in heart tissue from two groups were measured by immunoblotting and densitometric analyses (ND: n=3, HFD: n=3). (C) Histone acetylation levels were measured in cardiac fibroblasts and cardiac cells with or without fructose by immunoblotting and densitometric analyses. (ND: n=3, HFD: n=3). (D) The mRNA expression levels of Hdac 1 – 3 and Sirt 1 – 3 in the heart (ND: n=12, HFD: n=12). (E) The protein expression levels of Sirt1 in heart tissue by immunoblotting and densitometric analyses (ND: n=3, HFD: n=3). (F) The protein expression levels of Sirt1 by immunoblotting and densitometric analyses after fructose treatment for MEF and H9c2 cells (ND: n=3, HFD: n=3). ns, not significant (P≥0.05); *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001. HFD, high-fructose drinking; MEF, Mouse embryonic fibroblast; ND, normal diet.

Article Snippet: H9c2 (Rat myocardial cells), MEF (Mouse embryonic fibroblast; ATCC Number: SCRC-1008) and NIH/3T3 (MEF cells; ATCC Number: CRL-1658) cells were used in this study.

Techniques: Expressing, Western Blot

Journal: PLOS Pathogens

Article Title: Nucleus softens during herpesvirus infection

doi: 10.1371/journal.ppat.1013873

Figure Lengend Snippet: (A) Cryo-soft X-ray (SXT) tomography ortho-slices of noninfected (NI) and infected mouse embryonic fibroblast (MEF) cells at 8 hpi. Molecular densities are presented in a continuous grey scale representing linear absorption coefficient values ranging from 0.1 to 0.4 μm -1 (calibration bar). Scale bars, 2 μm. (B) Cross-sections of 3D SXT reconstructions of a noninfected and an infected cell. The high-density region (heterochromatin) is shown in blue, the low-density region (euchromatin) in yellow, the viral replication compartment (VRC/euchromatin) area in green, and the cytoplasm in grey. See also . Quantitative analysis of linear absorption coefficient (LAC) of the (C) low-density and (D) high-density region in noninfected and infected cells at 4 and 8 hpi (n = 6). (E) Representative cross-sections of focused ion beam scanning electron microscopy (FIB-SEM) images of noninfected and infected MEF cells at 8 hpi. The viral capsid (arrows) and nuclear pore complexes (arrowheads) are shown. Scale bars, 0.5 µm. See also . (F) Reconstruction of chromatin fiber thickness and (G) chromatin density as a function of the distance from the nuclear envelope in noninfected and infected cells. The color scale in (F) indicates high and low chromatin thickness (yellow-blue), and in (G) high and low density (yellow-blue). The error bars show the standard deviation. Statistical significance was determined using Tukey’s test, and the significance values are denoted as * (p < 0.05). Nonsignificant differences (p ≥ 0.05) are not labeled.

Article Snippet: Mouse embryonic fibroblast cells (MEF, ATCC CRL-2991) and African green monkey kidney cells (Vero, ATCC CCL-81) were grown in Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum, L-glutamine, and penicillin-streptomycin (Gibco-Invitrogen) at 37 °C in the presence of 5% CO 2.

Techniques: Tomography, Infection, Electron Microscopy, Standard Deviation, Labeling