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m4rt transport medium  (Thermo Fisher)


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    Structured Review

    Thermo Fisher m4rt transport medium
    M4rt Transport Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/medium/nct03277170-12-8-12
    Average 90 stars, based on 1 article reviews
    m4rt transport medium - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Vγ1 γδ T cells steer airway macrophages toward a profibrotic response in an autochthonous lung cancer mouse model.
    Article Snippet: Fresh agarose- inflated lungs were sliced into 300- μm- thick sections on a vibrating microtome (Campden Ltd.). .. For γδ Τ cell mapping, slices were blocked with TruStain FCX PLUS (BioLegend) and stained with anti TCRD AF647 (GL3, BioLegend), CD27 AF594 (LG.3A10, BioLegend), CD31 AF488 (390, BioLegend), and CD45 AF700 (30- F11, BioLegend) antibodies in complete medium (phenol red– free DMEM supplemented with 1% FBS, Gibco) for 20 min at 37°C. .. After washing, slices were fixed in 4% formaldehyde in PBS (VWR) for 20 min at room temperature.

    Article Title: Integrin-dependent neutrophil slowing reduces lung perfusion and supports metastasis in a model of breast cancer.
    Article Snippet: .. Lungs were sliced into 300μm thick sections on a vibratome and stained with directly conjugated antibodies, Hoechst and probes for reactive superoxide and nitric oxide (ROSID ROS/RNS detection kit, Enzo Life Sciences) in complete medium (phenol-red free DMEM (Thermo Fisher Scientific) substituted with 10% FBS (Thermo Fisher Scientific) for 20 minutes at 37◦C. .. For ex vivo integrins activation experiments, isotype control (clone RTK2758, Biolegend, Cat# 400565, RRID:AB_11148951) or CD18-activating (clone M18/2, Biolegend, Cat# 101419, RRID:AB_2832275) antibodies were added to the solution at 1:50 dilution.

    Concentration Assay:

    Article Title: Transdermal bicarbonate buffer therapy increases intratumoral pH and elicits antitumor responses in bladder cancer
    Article Snippet: T cells from C57BL/6 were stimulated with cell activation cocktail (BioLegend, #423303) for 6 h. .. CM was prewarmed to 37°C and divided into two. pH 6.6 medium was supplemented with 1 M of PIPES (Thermo Scientific, Waltham, Massachusetts, USA, cat# J62195.AK) to 25 mM final concentration; pH 7.4 medium was supplemented with 1 M of HEPES (Corning, ref# 25-060-CI) to 25 mM. pH values were adjusted using 1 N of NaOH (pH 7.4) or 1 N of HCl (pH 6.6) and measured using an Accumet AB150 pH benchtop meter (Thermo Scientific). .. Both media were sterilized through 0.22-μm filters (Corning) and then placed overnight in 5% CO 2 incubators for CO 2 equilibration. pH values were verified and readjusted, if necessary, the following day, and media were refiltered.

    Article Title: Epigenetic dysregulation and biological function of PDX1 in prostate cancer.
    Article Snippet: .. After 24 hours, the transfected cells were cultured in a medium containing puromycin (Thermo Fisher Scientific Cat No. J67236) at a final concentration of 3 μg/ml for selection. ..

    Modification:

    Article Title: New PPARG Exons: Cell-Specific Expression of Their RNAs in the Human Placenta.
    Article Snippet: .. The cell pellets were then suspended in Dulbecco′s modified Eagle′s medium (DMEM, containing 1 g/L glucose, pyruvate, without phenol red; Thermo Fisher Scientific, #11880, Illkirch, France; GibcoTM #11880-028) and layered on a preformed Percoll gradient (70% and 5%) (CytivaTM #17-5445-01, Uppsala, Sweden). ..

    Article Title: Assay integration for reproducible toxicity testing of particulate matter in a single well: cytotoxicity, oxidative stress, inflammatory response, and mRNA profiling.
    Article Snippet: .. Cell Culture Jo ur na l P re -p ro of A549 human alveolar type II epithelial-like and J774A.1 (referred to as J774) murine monocyte / macrophage cell lines (American Type Culture Collection, Manassas, VA, USA) were cultured and propagated in growth Dulbecco’s Modified Eagle’s Medium (DMEM, Thermo-Fisher; containing 2 mM L-glutamine, 4.5 g/L glucose, phenol red) supplemented with 10 % FBS (Cytiva, Canada), v/v in 75 cm2 tissue culture flasks (Corning, NY, USA). ..

    Cell Culture:

    Article Title: Assay integration for reproducible toxicity testing of particulate matter in a single well: cytotoxicity, oxidative stress, inflammatory response, and mRNA profiling.
    Article Snippet: .. Cell Culture Jo ur na l P re -p ro of A549 human alveolar type II epithelial-like and J774A.1 (referred to as J774) murine monocyte / macrophage cell lines (American Type Culture Collection, Manassas, VA, USA) were cultured and propagated in growth Dulbecco’s Modified Eagle’s Medium (DMEM, Thermo-Fisher; containing 2 mM L-glutamine, 4.5 g/L glucose, phenol red) supplemented with 10 % FBS (Cytiva, Canada), v/v in 75 cm2 tissue culture flasks (Corning, NY, USA). ..

    Article Title: Epigenetic dysregulation and biological function of PDX1 in prostate cancer.
    Article Snippet: .. After 24 hours, the transfected cells were cultured in a medium containing puromycin (Thermo Fisher Scientific Cat No. J67236) at a final concentration of 3 μg/ml for selection. ..

    Microscopy:

    Article Title: Gibberellin biosynthesis in Lotus japonicus regulates arbuscule distribution, but not overall colonisation by arbuscular mycorrhizal fungi
    Article Snippet: .. Roots were then mounted onto a microscope slide (ThermoFisher, USA) in mounting medium (20% v/v glycerol, 50 mM Tris-HCl, pH 7.5, and 0.1% v/v Tween-20 in sterile ultrapure water). ..

    Sterility:

    Article Title: Gibberellin biosynthesis in Lotus japonicus regulates arbuscule distribution, but not overall colonisation by arbuscular mycorrhizal fungi
    Article Snippet: .. Roots were then mounted onto a microscope slide (ThermoFisher, USA) in mounting medium (20% v/v glycerol, 50 mM Tris-HCl, pH 7.5, and 0.1% v/v Tween-20 in sterile ultrapure water). ..

    Suspension:

    Article Title: TYK-2 inhibitor
    Article Snippet: Assay C: IL-12-JAK2, TYK2/p-STAT4 (Tyr693) Inhibition IC50 Cellular Assay To evaluate the inhibition effect of compounds disclosed herein on JAK2, TYK2/p-STAT4 (Tyr693) activated by IL-12 in NK-92 cell line, NK-92 cells were collected and washed 3 times by DPBS and resuspended in MEM-α(GIBCO,Cat #12561056) with 10% FBS (Gibco, Cat #10099, Lot #1891605), without IL-2 (R&D systems,Cat #202-IL), starved overnight. .. Cells were collected and resuspended in 1640 medium (phenol red free, Gibco, Cat #11835-030) with 0.1% BSA, and 12.5 μl/5×104/well cell suspension were seeded to the 96-well plate (Corning, Cat #3799). ..

    Transfection:

    Article Title: Epigenetic dysregulation and biological function of PDX1 in prostate cancer.
    Article Snippet: .. After 24 hours, the transfected cells were cultured in a medium containing puromycin (Thermo Fisher Scientific Cat No. J67236) at a final concentration of 3 μg/ml for selection. ..

    Selection:

    Article Title: Epigenetic dysregulation and biological function of PDX1 in prostate cancer.
    Article Snippet: .. After 24 hours, the transfected cells were cultured in a medium containing puromycin (Thermo Fisher Scientific Cat No. J67236) at a final concentration of 3 μg/ml for selection. ..



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    Image Search Results


    In vitro evaluation of the osteogenic-promoting capability of BTO@ALN. (A–D) Immunofluorescence staining of osteoblasts differentiated from primary mouse bone marrow–derived mesenchymal stem cells (BMSCs) (red: actin; green: RUNX2 and ATF4) and corresponding quantitative analysis of fluorescence intensity. (E–H) Alkaline phosphatase (ALP) and Alizarin Red S (ARS) staining of osteoblasts derived from primary mouse BMSCs and quantitative analysis of staining intensity.

    Journal: Bioactive Materials

    Article Title: A motion-activated skeletal “Pacemaker” for systemic bone remodeling via oral piezoelectric microspheres

    doi: 10.1016/j.bioactmat.2026.05.051

    Figure Lengend Snippet: In vitro evaluation of the osteogenic-promoting capability of BTO@ALN. (A–D) Immunofluorescence staining of osteoblasts differentiated from primary mouse bone marrow–derived mesenchymal stem cells (BMSCs) (red: actin; green: RUNX2 and ATF4) and corresponding quantitative analysis of fluorescence intensity. (E–H) Alkaline phosphatase (ALP) and Alizarin Red S (ARS) staining of osteoblasts derived from primary mouse BMSCs and quantitative analysis of staining intensity.

    Article Snippet: Osteogenic differentiation was induced by culturing BMSCs in osteogenic induction medium (Procell, Wuhan, China).

    Techniques: In Vitro, Immunofluorescence, Staining, Derivative Assay, Fluorescence