Journal: iScience

Article Title: A unique poly(A) tail profile uncovers the stability and translational activation of TOP transcripts during neuronal differentiation

doi: 10.1016/j.isci.2023.107511

Figure Lengend Snippet:

Article Snippet: TaKaRa NDiff® 227 Medium , Takara , Cat# Y40002.

Techniques: Recombinant, Cell Viability Assay, Flow Cytometry, Bicinchoninic Acid Protein Assay, Purification, RNA Sequencing, Microscopy, Control, Multiplexing, Plasmid Preparation, Software, Imaging, Sequencing

Journal: Cell reports

Article Title: Genetic- and diet-induced ω -3 fatty acid enrichment enhances TRPV4-mediated vasodilation in mice

doi: 10.1016/j.celrep.2022.111306

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Endothelial Cell Growth Medium MV 2 , Promo Cell , C-22121.

Techniques: Recombinant, Protease Inhibitor, Staining, Western Blot, Membrane, Protein Extraction, Plasmid Preparation, Software, Imaging, Microscopy

Journal: Stem Cell Research & Therapy

Article Title: Identification of CD141 + vasculogenic precursor cells from human bone marrow and their endothelial engagement in the arteriogenesis by co-transplantation with mesenchymal stem cells

doi: 10.1186/s13287-024-03994-9

Figure Lengend Snippet: CD141 is a critical marker that distinguishes BM-VPCs from BM-MSCs. A List of markers with an expression difference of > 50% between BM-VPCs and BM-MSCs screened among 376 surface markers. B Differential expression of CD141 and CD282 in 15–20 donors assessed using flow cytometry analysis (*** p < 0.001, Student's t-test, two-tailed). C–F BM-MNCs were sorted using magnetic bead-conjugated CD141 antibody. The selected MNCs were plated in EGMPL. C Colonies that appeared from CD141 + sorted BM-MNCs on days 5, 7, and 9. Scale bar = 500 μm. D Marker expression profile of ex vivo-cultured cells from CD141 + sorted colonies at passages 1 to 4 analyzed using flow cytometry. E, F CD141 + sorted BM-MNCs were cultured in EGMPL at passage 0; from passage 1 to 3 and then, the cells were divided and cultured in EGMPL or StemMACS. E Morphology of replated CD141 + sorted cells in EGMPL and StemMACS at passages 1 to 3, and their tube-forming ability in MEMα + 0.2%hPL at passage 2. Scale bar = 500 μm. F Marker expression profile of CD141 + sorted cells cultured in StemMACS

Article Snippet: StemMACS is an abbreviation for StemMACS MSC expansion medium XF, human (Miltenyi Biotec, Bergisch Gladbach, Germany).

Techniques: Marker, Expressing, Quantitative Proteomics, Flow Cytometry, Two Tailed Test, Ex Vivo, Cell Culture