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mcf 7  (ATCC)


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    ATCC mcf 7
    Quantitative RT‐PCR validation of key subtype marker genes in breast cancer cell lines. Bar chart of relative mRNA expression levels of six marker genes (ERBB2, ESR1, PGR, KRT5, VIM, and CDH1) normalized to GAPDH <t>in</t> <t>MCF‐7</t> (luminal, blue bars) and MDA‐MB‐231 (basal/TNBC, red bars) cell lines. Error bars represent standard deviation from three independent biological replicates. Significance brackets indicate: ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 by two‐tailed Student′s t ‐test. Luminal markers ERBB2, ESR1, PGR, and CDH1 show significantly higher expression in MCF‐7, whereas basal/mesenchymal markers KRT5 and VIM are significantly enriched in MDA‐MB‐231, validating single‐cell sequencing findings.
    Mcf 7, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 35106 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mcf/MCF7/pmc13673301-91-0-3
    Average 99 stars, based on 35106 article reviews
    mcf 7 - by Bioz Stars, 2026-10
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    Images

    1) Product Images from "Single‐Cell Multiomics Integration Reveals Cell Trajectories and Regulatory Networks in Breast Cancer Progression"

    Article Title: Single‐Cell Multiomics Integration Reveals Cell Trajectories and Regulatory Networks in Breast Cancer Progression

    Journal: International Journal of Genomics

    doi: 10.1155/ijog/9324717

    Quantitative RT‐PCR validation of key subtype marker genes in breast cancer cell lines. Bar chart of relative mRNA expression levels of six marker genes (ERBB2, ESR1, PGR, KRT5, VIM, and CDH1) normalized to GAPDH in MCF‐7 (luminal, blue bars) and MDA‐MB‐231 (basal/TNBC, red bars) cell lines. Error bars represent standard deviation from three independent biological replicates. Significance brackets indicate: ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 by two‐tailed Student′s t ‐test. Luminal markers ERBB2, ESR1, PGR, and CDH1 show significantly higher expression in MCF‐7, whereas basal/mesenchymal markers KRT5 and VIM are significantly enriched in MDA‐MB‐231, validating single‐cell sequencing findings.
    Figure Legend Snippet: Quantitative RT‐PCR validation of key subtype marker genes in breast cancer cell lines. Bar chart of relative mRNA expression levels of six marker genes (ERBB2, ESR1, PGR, KRT5, VIM, and CDH1) normalized to GAPDH in MCF‐7 (luminal, blue bars) and MDA‐MB‐231 (basal/TNBC, red bars) cell lines. Error bars represent standard deviation from three independent biological replicates. Significance brackets indicate: ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 by two‐tailed Student′s t ‐test. Luminal markers ERBB2, ESR1, PGR, and CDH1 show significantly higher expression in MCF‐7, whereas basal/mesenchymal markers KRT5 and VIM are significantly enriched in MDA‐MB‐231, validating single‐cell sequencing findings.

    Techniques Used: Quantitative RT-PCR, Biomarker Discovery, Marker, Expressing, Standard Deviation, Two Tailed Test, Single-Cell Sequencing

    Related Articles

    Cell Culture:

    Article Title: Evaluation of the efficacy of rutin, rutin nanocrystals, and rutin spanlastics nanoparticles as antitumor and antioxidant drug delivery systems.
    Article Snippet: .. The MCF-7 human breast cancer model routinely utilized was obtained from the American Type Culture Collection (ATCC; USA) and cultured from the National Cancer Institute (NCI; Cairo, Egypt). .. Cells were subcultured in Dulbecco's Modified Eagle Medium (DMEM; Gibco, USA) with 10% heat-inactivated fetal bovine serum (FBS; Sigma-Aldrich, USA), 100 U/mL penicillin, and 100 μg/mL streptomycin (Lonza, Switzerland).

    Article Title: Dual Role of Cancer Epithelial-Specific TRAF3 in Regulating Breast Cancer Cell Survival and Lymphocyte Activity
    Article Snippet: Publicly available mRNA expression data was analyzed and visualized in breast cancer cohorts through cBioPortal [ , ] (TCGA-BRCA) and the GOBO on-line tool [ ] (Chi, GSE11121 , GSE12093 , GSE2034 , GSE2603 , GSE5327 , GSE6532 , GSE7390 , GSE12093 , GSE1456 , GSE3494 , GSE7390 ) presented in detail in . .. Breast cancer cell lines MDA-MB-231 and MCF-7 were purchased from ATCC (Manassas, VA, USA) and cultured in 37 °C, 5% CO 2 , in DMEM medium with 10% FBS and pen/strep. .. The TRAF3 coding sequence was amplified from a pCMV6-TRAF3-GFP (RG210417, Origene, Rockville, MD, USA) vector and cloned into pLenti-EF1a-GFP-2A-Puro (LV067, ABM Inc, Richmond, BC, Canada).

    Modification:

    Article Title: Equilibrium and non-equilibrium thermodynamics in drug repurposing: Machine learning-guided discovery of high-affinity WEE1 kinase inhibitors.
    Article Snippet: WEE1 kinase represents a promising therapeutic target in oncology due to its critical role in cell cycle checkpoint regulation.. Traditional drug discovery for WEE1 inhibitors has been constrained by the time and resource demands of conventional screening.. Here, we integrate machine learning with equilibrium and non-equilibrium thermodynamic analyses to identify potential WEE1 inhibitors from FDA-approved drug libraries.

    Multiple Displacement Amplification:

    Article Title: Sparingly PEGylated Adipate Copolymers via Enzymatic Synthesis as Nano-Carriers for Solid Dispersions.
    Article Snippet: .. The human epithelial cell line Caco-2, the MCF-7 human breast cancer cells, and the MDA-MB-231 late-stage triple-negative breast cancer cells were obtained from the American Type Culture Collection (ATCC) and used across a 10-passage window. ..

    Article Title: Dual Role of Cancer Epithelial-Specific TRAF3 in Regulating Breast Cancer Cell Survival and Lymphocyte Activity
    Article Snippet: Publicly available mRNA expression data was analyzed and visualized in breast cancer cohorts through cBioPortal [ , ] (TCGA-BRCA) and the GOBO on-line tool [ ] (Chi, GSE11121 , GSE12093 , GSE2034 , GSE2603 , GSE5327 , GSE6532 , GSE7390 , GSE12093 , GSE1456 , GSE3494 , GSE7390 ) presented in detail in . .. Breast cancer cell lines MDA-MB-231 and MCF-7 were purchased from ATCC (Manassas, VA, USA) and cultured in 37 °C, 5% CO 2 , in DMEM medium with 10% FBS and pen/strep. .. The TRAF3 coding sequence was amplified from a pCMV6-TRAF3-GFP (RG210417, Origene, Rockville, MD, USA) vector and cloned into pLenti-EF1a-GFP-2A-Puro (LV067, ABM Inc, Richmond, BC, Canada).



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    Image Search Results


    Quantitative RT‐PCR validation of key subtype marker genes in breast cancer cell lines. Bar chart of relative mRNA expression levels of six marker genes (ERBB2, ESR1, PGR, KRT5, VIM, and CDH1) normalized to GAPDH in MCF‐7 (luminal, blue bars) and MDA‐MB‐231 (basal/TNBC, red bars) cell lines. Error bars represent standard deviation from three independent biological replicates. Significance brackets indicate: ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 by two‐tailed Student′s t ‐test. Luminal markers ERBB2, ESR1, PGR, and CDH1 show significantly higher expression in MCF‐7, whereas basal/mesenchymal markers KRT5 and VIM are significantly enriched in MDA‐MB‐231, validating single‐cell sequencing findings.

    Journal: International Journal of Genomics

    Article Title: Single‐Cell Multiomics Integration Reveals Cell Trajectories and Regulatory Networks in Breast Cancer Progression

    doi: 10.1155/ijog/9324717

    Figure Lengend Snippet: Quantitative RT‐PCR validation of key subtype marker genes in breast cancer cell lines. Bar chart of relative mRNA expression levels of six marker genes (ERBB2, ESR1, PGR, KRT5, VIM, and CDH1) normalized to GAPDH in MCF‐7 (luminal, blue bars) and MDA‐MB‐231 (basal/TNBC, red bars) cell lines. Error bars represent standard deviation from three independent biological replicates. Significance brackets indicate: ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 by two‐tailed Student′s t ‐test. Luminal markers ERBB2, ESR1, PGR, and CDH1 show significantly higher expression in MCF‐7, whereas basal/mesenchymal markers KRT5 and VIM are significantly enriched in MDA‐MB‐231, validating single‐cell sequencing findings.

    Article Snippet: MCF‐7 (luminal, ER+/PR+/HER2‐, ATCC HTB‐22) and MDA‐MB‐231 (basal/TNBC, ATCC HTB‐26) cell lines were cultured in DMEM supplemented with 10% FBS, 100 U/mL penicillin, and 100 μ g/mL streptomycin at 37°C/5% CO2.

    Techniques: Quantitative RT-PCR, Biomarker Discovery, Marker, Expressing, Standard Deviation, Two Tailed Test, Single-Cell Sequencing

    Cytotoxicity of OcEO against tumors and non-tumor human cell lines. Dose–response curves for ( a ) MCF-7 (human breast adenocarcinoma), ( b ) HCT-116 (human colorectal carcinoma), and ( c ) MRC-5 (human normal lung fibroblasts). ( d ) IC 50 (concentration required to inhibit 50% of cell proliferation) comparison among the studied cell lines. R 2 : Goodness of fit for the sigmoidal dose–response analysis. Results are presented as mean ± SD from three independent experiments performed in triplicate. *: indicates significant differences between MRC5 and MCF-7, and between MRC5 and HCT-116 (ANOVA, followed by Dunnett’s post hoc test, p < 0.0001).

    Journal: Plants

    Article Title: Chemical Characterization and In Vitro Biological Activities of Essential Oil from Oxalis corniculata L. Collected in the Ecuador Highlands

    doi: 10.3390/plants15193047

    Figure Lengend Snippet: Cytotoxicity of OcEO against tumors and non-tumor human cell lines. Dose–response curves for ( a ) MCF-7 (human breast adenocarcinoma), ( b ) HCT-116 (human colorectal carcinoma), and ( c ) MRC-5 (human normal lung fibroblasts). ( d ) IC 50 (concentration required to inhibit 50% of cell proliferation) comparison among the studied cell lines. R 2 : Goodness of fit for the sigmoidal dose–response analysis. Results are presented as mean ± SD from three independent experiments performed in triplicate. *: indicates significant differences between MRC5 and MCF-7, and between MRC5 and HCT-116 (ANOVA, followed by Dunnett’s post hoc test, p < 0.0001).

    Article Snippet: The human breast adenocarcinoma cell line MCF-7 (ATCC, Manassas, VA, USA), the human colorectal cancer cell line HCT-116 (ECACC, Saint Louis, MO, USA), and the human normal lung fibroblast cell line MRC-5 (ABAC, Cordoba, Argentina) were cultured in DMEM (Merck, Darmstadt, Germany) supplemented with 10% fetal bovine serum (Internegocios, Cordoba, Argentina),100 IU/mL of penicillin and 100 μg/mL of streptomycin (GIBCO, NY, USA).

    Techniques: Concentration Assay, Comparison, R-Squared, Analysis

    Cytotoxic activity of OcEO.

    Journal: Plants

    Article Title: Chemical Characterization and In Vitro Biological Activities of Essential Oil from Oxalis corniculata L. Collected in the Ecuador Highlands

    doi: 10.3390/plants15193047

    Figure Lengend Snippet: Cytotoxic activity of OcEO.

    Article Snippet: The human breast adenocarcinoma cell line MCF-7 (ATCC, Manassas, VA, USA), the human colorectal cancer cell line HCT-116 (ECACC, Saint Louis, MO, USA), and the human normal lung fibroblast cell line MRC-5 (ABAC, Cordoba, Argentina) were cultured in DMEM (Merck, Darmstadt, Germany) supplemented with 10% fetal bovine serum (Internegocios, Cordoba, Argentina),100 IU/mL of penicillin and 100 μg/mL of streptomycin (GIBCO, NY, USA).

    Techniques: Activity Assay

    Cytotoxicity (IC 50 μg/mL) of LEO and SEO.

    Journal: Molecules

    Article Title: Phytochemical Analysis and Biological Activities of Essential Oils from Leaves and Stems of Dicranopteris pedata (Houtt.) Nakaike

    doi: 10.3390/molecules31193518

    Figure Lengend Snippet: Cytotoxicity (IC 50 μg/mL) of LEO and SEO.

    Article Snippet: A panel of human cancer cell lines—HepG2 ATCC HB-8065 (liver), MCF-7 ATCC HTB-22 (breast), A-549 ATCC CCL-185 (lung), and HCT-116 ATCC CCL-247 (colon)—along with the normal hepatic line LO2 (RRID CVCL_6926) was acquired from the Shanghai Institute for Biological Sciences (SIBS), located in Shanghai, China.

    Techniques:

    Selectivity index (SI) of LEO and SEO against different cell lines.

    Journal: Molecules

    Article Title: Phytochemical Analysis and Biological Activities of Essential Oils from Leaves and Stems of Dicranopteris pedata (Houtt.) Nakaike

    doi: 10.3390/molecules31193518

    Figure Lengend Snippet: Selectivity index (SI) of LEO and SEO against different cell lines.

    Article Snippet: A panel of human cancer cell lines—HepG2 ATCC HB-8065 (liver), MCF-7 ATCC HTB-22 (breast), A-549 ATCC CCL-185 (lung), and HCT-116 ATCC CCL-247 (colon)—along with the normal hepatic line LO2 (RRID CVCL_6926) was acquired from the Shanghai Institute for Biological Sciences (SIBS), located in Shanghai, China.

    Techniques: