Review



cd25  (Bio-Rad)


Bioz Verified Symbol Bio-Rad is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Bio-Rad cd25
    Proportions of regulatory and conventional CD4 T cells in BALC. A – D The percentages of Tconv and Treg CD4 cells in the BALC were determined using flow cytometry. (A, B) presents the percentages within total CD3 + CD4 + cells. A Shows CD4 + <t>CD25</t> −/low Foxp3 − Tconv cells. B Shows CD4 + CD25 high Foxp3 + Treg cells. C , D Shows the percentages of ICOS + Ki-67 + within the respective parental population of C Tconv and D Treg. Statistical analysis was performed using the Mann–Whitney unpaired, non-parametric test, (*) p < 0.05 or (**) p < 0.01 (mean; n = 5).
    Cd25, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 88 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd25/product/Bio-Rad
    Average 93 stars, based on 88 article reviews
    cd25 - by Bioz Stars, 2026-02
    93/100 stars

    Images

    1) Product Images from "Swine influenza A virus infection sets the local immunological landscape in subsequent infection with porcine reproductive and respiratory syndrome virus"

    Article Title: Swine influenza A virus infection sets the local immunological landscape in subsequent infection with porcine reproductive and respiratory syndrome virus

    Journal: Veterinary Research

    doi: 10.1186/s13567-025-01536-6

    Proportions of regulatory and conventional CD4 T cells in BALC. A – D The percentages of Tconv and Treg CD4 cells in the BALC were determined using flow cytometry. (A, B) presents the percentages within total CD3 + CD4 + cells. A Shows CD4 + CD25 −/low Foxp3 − Tconv cells. B Shows CD4 + CD25 high Foxp3 + Treg cells. C , D Shows the percentages of ICOS + Ki-67 + within the respective parental population of C Tconv and D Treg. Statistical analysis was performed using the Mann–Whitney unpaired, non-parametric test, (*) p < 0.05 or (**) p < 0.01 (mean; n = 5).
    Figure Legend Snippet: Proportions of regulatory and conventional CD4 T cells in BALC. A – D The percentages of Tconv and Treg CD4 cells in the BALC were determined using flow cytometry. (A, B) presents the percentages within total CD3 + CD4 + cells. A Shows CD4 + CD25 −/low Foxp3 − Tconv cells. B Shows CD4 + CD25 high Foxp3 + Treg cells. C , D Shows the percentages of ICOS + Ki-67 + within the respective parental population of C Tconv and D Treg. Statistical analysis was performed using the Mann–Whitney unpaired, non-parametric test, (*) p < 0.05 or (**) p < 0.01 (mean; n = 5).

    Techniques Used: Flow Cytometry, MANN-WHITNEY



    Similar Products

    cd25  (Bio-Rad)
    93
    Bio-Rad cd25
    Proportions of regulatory and conventional CD4 T cells in BALC. A – D The percentages of Tconv and Treg CD4 cells in the BALC were determined using flow cytometry. (A, B) presents the percentages within total CD3 + CD4 + cells. A Shows CD4 + <t>CD25</t> −/low Foxp3 − Tconv cells. B Shows CD4 + CD25 high Foxp3 + Treg cells. C , D Shows the percentages of ICOS + Ki-67 + within the respective parental population of C Tconv and D Treg. Statistical analysis was performed using the Mann–Whitney unpaired, non-parametric test, (*) p < 0.05 or (**) p < 0.01 (mean; n = 5).
    Cd25, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd25/product/Bio-Rad
    Average 93 stars, based on 1 article reviews
    cd25 - by Bioz Stars, 2026-02
    93/100 stars
    		  Buy from Supplier
    anti rat cd25 alexa flour 647  (Bio-Rad)
    93
    Bio-Rad anti rat cd25 alexa flour 647
    Proportions of regulatory and conventional CD4 T cells in BALC. A – D The percentages of Tconv and Treg CD4 cells in the BALC were determined using flow cytometry. (A, B) presents the percentages within total CD3 + CD4 + cells. A Shows CD4 + <t>CD25</t> −/low Foxp3 − Tconv cells. B Shows CD4 + CD25 high Foxp3 + Treg cells. C , D Shows the percentages of ICOS + Ki-67 + within the respective parental population of C Tconv and D Treg. Statistical analysis was performed using the Mann–Whitney unpaired, non-parametric test, (*) p < 0.05 or (**) p < 0.01 (mean; n = 5).
    Anti Rat Cd25 Alexa Flour 647, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti rat cd25 alexa flour 647/product/Bio-Rad
    Average 93 stars, based on 1 article reviews
    anti rat cd25 alexa flour 647 - by Bioz Stars, 2026-02
    93/100 stars
    		  Buy from Supplier
    d4 cd25 dual color  (Bio-Rad)
    93
    Bio-Rad d4 cd25 dual color
    Proportions of regulatory and conventional CD4 T cells in BALC. A – D The percentages of Tconv and Treg CD4 cells in the BALC were determined using flow cytometry. (A, B) presents the percentages within total CD3 + CD4 + cells. A Shows CD4 + <t>CD25</t> −/low Foxp3 − Tconv cells. B Shows CD4 + CD25 high Foxp3 + Treg cells. C , D Shows the percentages of ICOS + Ki-67 + within the respective parental population of C Tconv and D Treg. Statistical analysis was performed using the Mann–Whitney unpaired, non-parametric test, (*) p < 0.05 or (**) p < 0.01 (mean; n = 5).
    D4 Cd25 Dual Color, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/d4 cd25 dual color/product/Bio-Rad
    Average 93 stars, based on 1 article reviews
    d4 cd25 dual color - by Bioz Stars, 2026-02
    93/100 stars
    		  Buy from Supplier
    anti cd25  (Bio-Rad)
    93
    Bio-Rad anti cd25
    Proportions of regulatory and conventional CD4 T cells in BALC. A – D The percentages of Tconv and Treg CD4 cells in the BALC were determined using flow cytometry. (A, B) presents the percentages within total CD3 + CD4 + cells. A Shows CD4 + <t>CD25</t> −/low Foxp3 − Tconv cells. B Shows CD4 + CD25 high Foxp3 + Treg cells. C , D Shows the percentages of ICOS + Ki-67 + within the respective parental population of C Tconv and D Treg. Statistical analysis was performed using the Mann–Whitney unpaired, non-parametric test, (*) p < 0.05 or (**) p < 0.01 (mean; n = 5).
    Anti Cd25, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd25/product/Bio-Rad
    Average 93 stars, based on 1 article reviews
    anti cd25 - by Bioz Stars, 2026-02
    93/100 stars
    		  Buy from Supplier
    rat cd25  (Bio-Rad)
    93
    Bio-Rad rat cd25
    Fig. 5. Representative immunohistochemistry staining of CD4, CD8, and <t>CD25</t> in corneal allografts. The numbers of the cells
    Rat Cd25, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rat cd25/product/Bio-Rad
    Average 93 stars, based on 1 article reviews
    rat cd25 - by Bioz Stars, 2026-02
    93/100 stars
    		  Buy from Supplier
    il 2r cells  (Bio-Rad)
    93
    Bio-Rad il 2r cells
    Fig. 5. Representative immunohistochemistry staining of CD4, CD8, and <t>CD25</t> in corneal allografts. The numbers of the cells
    Il 2r Cells, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/il 2r cells/product/Bio-Rad
    Average 93 stars, based on 1 article reviews
    il 2r cells - by Bioz Stars, 2026-02
    93/100 stars
    		  Buy from Supplier
    mca273pe  (Bio-Rad)
    93
    Bio-Rad mca273pe
    Antibodies used for flow cytometry and brain immunohistochemistry analysis in rat
    Mca273pe, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mca273pe/product/Bio-Rad
    Average 93 stars, based on 1 article reviews
    mca273pe - by Bioz Stars, 2026-02
    93/100 stars
    		  Buy from Supplier
    mouse anti rat cd25 rpe fc bio rad mca273pe 10 mouse anti rat cd3 alexa fluor 647 fc bio rad mca772a647 11 anti rat cd45 fitc  (Bio-Rad)
    93
    Bio-Rad mouse anti rat cd25 rpe fc bio rad mca273pe 10 mouse anti rat cd3 alexa fluor 647 fc bio rad mca772a647 11 anti rat cd45 fitc
    Antibodies used for flow cytometry and brain immunohistochemistry analysis in rat
    Mouse Anti Rat Cd25 Rpe Fc Bio Rad Mca273pe 10 Mouse Anti Rat Cd3 Alexa Fluor 647 Fc Bio Rad Mca772a647 11 Anti Rat Cd45 Fitc, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti rat cd25 rpe fc bio rad mca273pe 10 mouse anti rat cd3 alexa fluor 647 fc bio rad mca772a647 11 anti rat cd45 fitc/product/Bio-Rad
    Average 93 stars, based on 1 article reviews
    mouse anti rat cd25 rpe fc bio rad mca273pe 10 mouse anti rat cd3 alexa fluor 647 fc bio rad mca772a647 11 anti rat cd45 fitc - by Bioz Stars, 2026-02
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Proportions of regulatory and conventional CD4 T cells in BALC. A – D The percentages of Tconv and Treg CD4 cells in the BALC were determined using flow cytometry. (A, B) presents the percentages within total CD3 + CD4 + cells. A Shows CD4 + CD25 −/low Foxp3 − Tconv cells. B Shows CD4 + CD25 high Foxp3 + Treg cells. C , D Shows the percentages of ICOS + Ki-67 + within the respective parental population of C Tconv and D Treg. Statistical analysis was performed using the Mann–Whitney unpaired, non-parametric test, (*) p < 0.05 or (**) p < 0.01 (mean; n = 5).

    Journal: Veterinary Research

    Article Title: Swine influenza A virus infection sets the local immunological landscape in subsequent infection with porcine reproductive and respiratory syndrome virus

    doi: 10.1186/s13567-025-01536-6

    Figure Lengend Snippet: Proportions of regulatory and conventional CD4 T cells in BALC. A – D The percentages of Tconv and Treg CD4 cells in the BALC were determined using flow cytometry. (A, B) presents the percentages within total CD3 + CD4 + cells. A Shows CD4 + CD25 −/low Foxp3 − Tconv cells. B Shows CD4 + CD25 high Foxp3 + Treg cells. C , D Shows the percentages of ICOS + Ki-67 + within the respective parental population of C Tconv and D Treg. Statistical analysis was performed using the Mann–Whitney unpaired, non-parametric test, (*) p < 0.05 or (**) p < 0.01 (mean; n = 5).

    Article Snippet: CD25 , K231.3B2 , Mouse IgG1 , A647 , 1/10 , Bio-Rad.

    Techniques: Flow Cytometry, MANN-WHITNEY

    Fig. 5. Representative immunohistochemistry staining of CD4, CD8, and CD25 in corneal allografts. The numbers of the cells

    Journal: Frontiers in bioscience (Landmark edition)

    Article Title: Mesenchymal stem cell derived exosomes-based immunological signature in a rat model of corneal allograft rejection therapy.

    doi: 10.31083/j.fbl2703086

    Figure Lengend Snippet: Fig. 5. Representative immunohistochemistry staining of CD4, CD8, and CD25 in corneal allografts. The numbers of the cells

    Article Snippet: Subsequently, sections were pre-blocked with 5% goat serum albumin for 30 min and incubated with mouse anti-CD4, anti-CD8, anti-CD25 overnight at 4 ◦C (Mouse anti Rat CD4 (MCA55GA, 1:50, AbD Serotec), Mouse anti Rat CD8 (MCA48GA, 1:500, AbD Serotec), Mouse anti Rat CD25 (MCA273GA, 1:100, AbD Serotec).

    Techniques: Immunohistochemistry, Staining

    Fig. 6. Flow cytometry was used for analysis of proportion of CD4+CD25+Foxp3+Tregs. Splenocytes from recipient rats were

    Journal: Frontiers in bioscience (Landmark edition)

    Article Title: Mesenchymal stem cell derived exosomes-based immunological signature in a rat model of corneal allograft rejection therapy.

    doi: 10.31083/j.fbl2703086

    Figure Lengend Snippet: Fig. 6. Flow cytometry was used for analysis of proportion of CD4+CD25+Foxp3+Tregs. Splenocytes from recipient rats were

    Article Snippet: Subsequently, sections were pre-blocked with 5% goat serum albumin for 30 min and incubated with mouse anti-CD4, anti-CD8, anti-CD25 overnight at 4 ◦C (Mouse anti Rat CD4 (MCA55GA, 1:50, AbD Serotec), Mouse anti Rat CD8 (MCA48GA, 1:500, AbD Serotec), Mouse anti Rat CD25 (MCA273GA, 1:100, AbD Serotec).

    Techniques: Flow Cytometry

    Antibodies used for flow cytometry and brain immunohistochemistry analysis in rat

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    Article Title: Elevated bone marrow sympathetic drive precedes systemic inflammation in angiotensin II hypertension

    doi: 10.1152/ajpheart.00510.2018

    Figure Lengend Snippet: Antibodies used for flow cytometry and brain immunohistochemistry analysis in rat

    Article Snippet: 9 , Mouse anti-rat CD25:RPE , FC , Bio-Rad , mca273pe.

    Techniques: Flow Cytometry, Immunohistochemistry

    Antibodies used for flow cytometry and brain immunohistochemistry analysis in rat

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    Article Title: Elevated bone marrow sympathetic drive precedes systemic inflammation in angiotensin II hypertension

    doi: 10.1152/ajpheart.00510.2018

    Figure Lengend Snippet: Antibodies used for flow cytometry and brain immunohistochemistry analysis in rat

    Article Snippet: All samples were read using an LSR-II (BD Biosystems) at the University of Florida Interdisciplinary Center for Biotechnology, and the data were analyzed with FACSDiva software, version 6.1.2. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Antibody Application Vendor Reference No. 1 Mmouse anti-NeuN IHC Millipore Sigma MAB377 2 Anti-Iba1 IHC Wako Laboratory 019-19741 3 Mouse anti-rat CD4 IHC ebioscience 14-0040-82 4 Mouse anti-rat CD68:Alexa Fluor 647 FC Bio-Rad mca341a647 6 Mouse anti-rat CD5:Alexa Fluor 647 FC Bio-Rad mca52a647 7 Mouse anti-rat CD8 ALPHA:Alexa Fluor 647 FC Bio-Rad mca48a647 8 Anti-Mouse/Rat CD90.1 (Thy-1.1) PerCP-Cyanine5.5 FC ebioscience 45-0900-80 9 Mouse anti-rat CD25:RPE FC Bio-Rad mca273pe 10 Mouse anti-rat CD3:Alexa Fluor 647 FC Bio-Rad mca772a647 11 Anti-Rat CD45 FITC, OXO1 FC ebioscience 11-0461-82 13 Anti-Mouse/Rat IL-17A Alexa Fluor 488 FC ebioscience 537177-81 14 Mouse anti-rat CD68:RPE FC Bio-Rad mca341pe 15 Mouse anti-rat CD4 647 FC Bio-Rad mca55ga Open in a separate window FC, flow cytometry; IHC, immunohistochemistry.

    Techniques: Flow Cytometry, Immunohistochemistry

    Central administration of ANG II is associated with elevated bone marrow (BM) and circulating inflammatory markers. Intracerebroventricular (ICV) ANG II increased levels of CD4+.IL17+ T cells in the blood (A and B) and BM (C) at day 7, as measured by flow cytometry. In addition, levels of several other T cell subtypes (CD4+.CD25+, CD3+, CD3+.CD45+, and CD68+ macrophage/monocytes were increased in the BM at day 7 of ANG II infusion (C), as measured by flow cytometry. Dennervation of the femoral BM sympathetic nerve (BM SNX) at day 7 of ICV ANG II infusion significantly reduced the ANG II-dependent increase in the inflammatory cells in the BM (C), suggesting BM sympathetic nerve activity-dependent activation of BM immune system by ANG II, as measured by flow cytometry. Real-time PCR showed elevated levels of several inflammatory markers, including TLR4, HIF1a, and ITGAM, in the BM at day 7 of ANG II infusion (D), which was reduced by BM SNX (left); significant elevation in several adrenergic receptors in the BM at day 21 of ANG II infusion, as measured by real-time PCR (right). Data are presented as means ± SE, n = 6; *P < 0.05 vs. control; #P < 0.05 vs. day 7; ANOVA.

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    Article Title: Elevated bone marrow sympathetic drive precedes systemic inflammation in angiotensin II hypertension

    doi: 10.1152/ajpheart.00510.2018

    Figure Lengend Snippet: Central administration of ANG II is associated with elevated bone marrow (BM) and circulating inflammatory markers. Intracerebroventricular (ICV) ANG II increased levels of CD4+.IL17+ T cells in the blood (A and B) and BM (C) at day 7, as measured by flow cytometry. In addition, levels of several other T cell subtypes (CD4+.CD25+, CD3+, CD3+.CD45+, and CD68+ macrophage/monocytes were increased in the BM at day 7 of ANG II infusion (C), as measured by flow cytometry. Dennervation of the femoral BM sympathetic nerve (BM SNX) at day 7 of ICV ANG II infusion significantly reduced the ANG II-dependent increase in the inflammatory cells in the BM (C), suggesting BM sympathetic nerve activity-dependent activation of BM immune system by ANG II, as measured by flow cytometry. Real-time PCR showed elevated levels of several inflammatory markers, including TLR4, HIF1a, and ITGAM, in the BM at day 7 of ANG II infusion (D), which was reduced by BM SNX (left); significant elevation in several adrenergic receptors in the BM at day 21 of ANG II infusion, as measured by real-time PCR (right). Data are presented as means ± SE, n = 6; *P < 0.05 vs. control; #P < 0.05 vs. day 7; ANOVA.

    Article Snippet: All samples were read using an LSR-II (BD Biosystems) at the University of Florida Interdisciplinary Center for Biotechnology, and the data were analyzed with FACSDiva software, version 6.1.2. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Antibody Application Vendor Reference No. 1 Mmouse anti-NeuN IHC Millipore Sigma MAB377 2 Anti-Iba1 IHC Wako Laboratory 019-19741 3 Mouse anti-rat CD4 IHC ebioscience 14-0040-82 4 Mouse anti-rat CD68:Alexa Fluor 647 FC Bio-Rad mca341a647 6 Mouse anti-rat CD5:Alexa Fluor 647 FC Bio-Rad mca52a647 7 Mouse anti-rat CD8 ALPHA:Alexa Fluor 647 FC Bio-Rad mca48a647 8 Anti-Mouse/Rat CD90.1 (Thy-1.1) PerCP-Cyanine5.5 FC ebioscience 45-0900-80 9 Mouse anti-rat CD25:RPE FC Bio-Rad mca273pe 10 Mouse anti-rat CD3:Alexa Fluor 647 FC Bio-Rad mca772a647 11 Anti-Rat CD45 FITC, OXO1 FC ebioscience 11-0461-82 13 Anti-Mouse/Rat IL-17A Alexa Fluor 488 FC ebioscience 537177-81 14 Mouse anti-rat CD68:RPE FC Bio-Rad mca341pe 15 Mouse anti-rat CD4 647 FC Bio-Rad mca55ga Open in a separate window FC, flow cytometry; IHC, immunohistochemistry.

    Techniques: Flow Cytometry, Activity Assay, Activation Assay, Real-time Polymerase Chain Reaction