Journal: bioRxiv
Article Title: Glial cell and perineuronal net interactions in the dorsal striatum of aged mice
doi: 10.64898/2026.03.25.714174
Figure Lengend Snippet: A Quantification of Iba1 + cell counts for the total striatum. Data was normalized to area. B. Quantification of Iba1 + intensity per Iba1 + cell. C. Quantification of CD68 + intensity per Iba1 + cell D. Quantification of WFA + intensity per Iba1 + cell. E. Linear regression analysis of Ibal + intensity versus CD68 + intensity per cell. F. Linear regression analysis of Ibal + intensity versus UTA + intensity per cell. G. Linear regression analysis of CD68 + intensity versus UTA + intensity per cell. A Data represented as mean ± SENL data was tested for normality prior to statistical analysis. Two-tailed t-tests or Welch’s t-test used when appropriate. Regression analysis: R 2 values of corresponding linear regressions label on graph and t-test comparison of slopes used, p-values: * <0.05, * * <0.01. N - 4-5 mice/group, 3-5 sections/mouse, 13-41 cells/mouse.
Article Snippet: Coronal 30-μm hemisections containing the dorsal striatum were processed in two independent staining/imaging cohorts: ( ) microglia were labeled with Iba1 (rabbit; FUJIFILM Wako, #P3088) in combination with the lysosomal/phagocytic marker CD68 (rat; Bio-Rad, #MCA1957) and perineuronal nets (PNNs) were visualized using fluorescein-conjugated Wisteria floribunda agglutinin (WFA; Vector Laboratories, #FL-1351); ( ) astrocytes were labeled with GFAP (rabbit; Cell Signaling Technology, #12389) together with WFA to visualize PNNs.
Techniques: Two Tailed Test, Comparison