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mca1788 rrid ab 322791  (Bio-Rad)


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    Bio-Rad mca1788 rrid ab 322791
    Mca1788 Rrid Ab 322791, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mca1788 rrid ab 322791/product/Bio-Rad
    Average 93 stars, based on 19 article reviews
    mca1788 rrid ab 322791 - by Bioz Stars, 2026-03
    93/100 stars

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    Automated single‐cell analysis. To determine whether single cells could be stained with FAST probes and analyzed through fingerprinting, we examined BT474 <t>(HER2+/ER+/PR+)</t> and MD‐MB‐231 cells (triple negative breast cancer). A) Representative examples of BT747 stained for the markers shown (left) and automated analysis of biomarker expression after fingerprint correction (right). Note the high levels of HER2 and ER/PR. B) Examples of triple‐negative MD‐MB‐231 cells stained for the same markers and showing low levels of HER2 and ER/PR, but positive signals from cancer markers (EpCAM, MUC1). C) Correlation between i2SCAN measurements and flow cytometry. Note the good correlation (Pearson r = 0.90). D) i2SCAN analysis of tumor cell/PBMC mixture displayed CD45 versus QUAD (HER2/EpCAM/MUC1/EGFR). Note the clean separation (Raw images in panels A and B are brightness and contrast adjusted).
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    Automated single‐cell analysis. To determine whether single cells could be stained with FAST probes and analyzed through fingerprinting, we examined BT474 (HER2+/ER+/PR+) and MD‐MB‐231 cells (triple negative breast cancer). A) Representative examples of BT747 stained for the markers shown (left) and automated analysis of biomarker expression after fingerprint correction (right). Note the high levels of HER2 and ER/PR. B) Examples of triple‐negative MD‐MB‐231 cells stained for the same markers and showing low levels of HER2 and ER/PR, but positive signals from cancer markers (EpCAM, MUC1). C) Correlation between i2SCAN measurements and flow cytometry. Note the good correlation (Pearson r = 0.90). D) i2SCAN analysis of tumor cell/PBMC mixture displayed CD45 versus QUAD (HER2/EpCAM/MUC1/EGFR). Note the clean separation (Raw images in panels A and B are brightness and contrast adjusted).

    Journal: Advanced Science

    Article Title: Integrated Analytical System for Clinical Single‐Cell Analysis

    doi: 10.1002/advs.202200415

    Figure Lengend Snippet: Automated single‐cell analysis. To determine whether single cells could be stained with FAST probes and analyzed through fingerprinting, we examined BT474 (HER2+/ER+/PR+) and MD‐MB‐231 cells (triple negative breast cancer). A) Representative examples of BT747 stained for the markers shown (left) and automated analysis of biomarker expression after fingerprint correction (right). Note the high levels of HER2 and ER/PR. B) Examples of triple‐negative MD‐MB‐231 cells stained for the same markers and showing low levels of HER2 and ER/PR, but positive signals from cancer markers (EpCAM, MUC1). C) Correlation between i2SCAN measurements and flow cytometry. Note the good correlation (Pearson r = 0.90). D) i2SCAN analysis of tumor cell/PBMC mixture displayed CD45 versus QUAD (HER2/EpCAM/MUC1/EGFR). Note the clean separation (Raw images in panels A and B are brightness and contrast adjusted).

    Article Snippet: 3 , HER2 , Tumor cells , BioRad , MCA1788 , ICR55 , Rat , BT474, HCC‐1954.

    Techniques: Single-cell Analysis, Staining, Biomarker Discovery, Expressing, Flow Cytometry

    Antibodies for flow and direct single cell staining

    Journal: Advanced Science

    Article Title: Integrated Analytical System for Clinical Single‐Cell Analysis

    doi: 10.1002/advs.202200415

    Figure Lengend Snippet: Antibodies for flow and direct single cell staining

    Article Snippet: 3 , HER2 , Tumor cells , BioRad , MCA1788 , ICR55 , Rat , BT474, HCC‐1954.

    Techniques: Control

    FAST antibodies for staining of paraffin‐embedded tissue sections (FFPE)

    Journal: Advanced Science

    Article Title: Integrated Analytical System for Clinical Single‐Cell Analysis

    doi: 10.1002/advs.202200415

    Figure Lengend Snippet: FAST antibodies for staining of paraffin‐embedded tissue sections (FFPE)

    Article Snippet: 3 , HER2 , Tumor cells , BioRad , MCA1788 , ICR55 , Rat , BT474, HCC‐1954.

    Techniques: Staining

    i2SCAN of biomarkers in FFPE sections. Tissue microarrays representing 24 patients with pathology proven breast cancer and variable expression levels of HER2, ER, and PR were analyzed by i2SCAN. Shown are representative examples for biomarker positive and negative cases. A) Synthetic images of representative cases positive for the biomarker shown and which was corroborated with IHC, FISH, and other testing. B) Synthetic images of representative cases confirmed negative for the biomarker shown. C) Summary plots of signal in segmented tumor cells. The threshold for a given marker was determined in representative samples (with and without controls).

    Journal: Advanced Science

    Article Title: Integrated Analytical System for Clinical Single‐Cell Analysis

    doi: 10.1002/advs.202200415

    Figure Lengend Snippet: i2SCAN of biomarkers in FFPE sections. Tissue microarrays representing 24 patients with pathology proven breast cancer and variable expression levels of HER2, ER, and PR were analyzed by i2SCAN. Shown are representative examples for biomarker positive and negative cases. A) Synthetic images of representative cases positive for the biomarker shown and which was corroborated with IHC, FISH, and other testing. B) Synthetic images of representative cases confirmed negative for the biomarker shown. C) Summary plots of signal in segmented tumor cells. The threshold for a given marker was determined in representative samples (with and without controls).

    Article Snippet: 3 , HER2 , Tumor cells , BioRad , MCA1788 , ICR55 , Rat , BT474, HCC‐1954.

    Techniques: Expressing, Biomarker Discovery, Marker

    Analysis of cellular biomarkers in patient cohort. A) Synthetic images derived from FFPE tissue section. Note the exclusive separation of tumor and host cells and the ability to analyze individual cells for markers. Top right: Mosaic of individual cancer cells that express multiple markers (red; positive for HER2, ER, PR, and/or Ki67), Ki67 only (yellow), HER2 only (purple), ER/PR only (green), or no HER2/ER/PR/Ki67 (orange). B) Summary of biomarker expression in one patient sample. A total of 14 markers were analyzed. C) Summary of HER2 expression across patient cohort (n = 19 patients). D) Summary of ER expression across patient cohort (n = 19 patients).

    Journal: Advanced Science

    Article Title: Integrated Analytical System for Clinical Single‐Cell Analysis

    doi: 10.1002/advs.202200415

    Figure Lengend Snippet: Analysis of cellular biomarkers in patient cohort. A) Synthetic images derived from FFPE tissue section. Note the exclusive separation of tumor and host cells and the ability to analyze individual cells for markers. Top right: Mosaic of individual cancer cells that express multiple markers (red; positive for HER2, ER, PR, and/or Ki67), Ki67 only (yellow), HER2 only (purple), ER/PR only (green), or no HER2/ER/PR/Ki67 (orange). B) Summary of biomarker expression in one patient sample. A total of 14 markers were analyzed. C) Summary of HER2 expression across patient cohort (n = 19 patients). D) Summary of ER expression across patient cohort (n = 19 patients).

    Article Snippet: 3 , HER2 , Tumor cells , BioRad , MCA1788 , ICR55 , Rat , BT474, HCC‐1954.

    Techniques: Derivative Assay, Biomarker Discovery, Expressing