Journal: Brazilian Journal of Veterinary Medicine
Article Title: Addition of synthetic polymer in the freezing solution of mesenchymal stem cells from equine adipose tissue as a future perspective for reducing of DMSO concentration
doi: 10.29374/2527-2179.bjvm002523
Figure Lengend Snippet: Immunophenotypic analysis of fresh eAT-MSCs, showed the reactivity, (A, B) with high positive expression of mesenchymal stem cells markers (CD90 and CD44) but almost no expression for hematopoietic markes (CD86, MHC-1 and MHC-II).
Article Snippet: For the immunophenotyping, it was used the following primary antibodies: mouse monoclonal CD90 (SC – 6071, Santa Cruz, Dallas, USA), mouse monoclonal CD44 (MCA1082GA, AbDSerotek, Raleigh, NC, USA), mouse MHC (Major Histocompatibility Complex) class I monomorphic (MCA1086GA, AbDSerotek, Raleigh, NC, USA), mouse monoclonal CD86 (NB100-77815, BD Pharmigen, São Paulo, Brazil) e mouse MHC Class II Monomorphic (MCA1085GA, AbDSerotek, Raleigh, NC, USA).
Techniques: Expressing