cd59  (Bio-Rad)

Journal: Journal of Personalized Medicine

Article Title: In Vitro Investigation of Pulsed Electromagnetic Field Stimulation (PEMF) with MAGCELL ® ARTHRO on the Regulatory Expression of Soluble and Membrane-Bound Complement Factors and Inflammatory Cytokines in Immortalized Synovial Fibroblasts

doi: 10.3390/jpm14070701

Figure Lengend Snippet: Oligonucleotides used for qPCR analysis.

Article Snippet: Mouse anti-human CD59 , Bio-Rad, Bio-Rad, Feldkirchen, Germany , MCA1054 , 1 mg/mL , 1:50.

Techniques: Sequencing, Amplification

Journal: Journal of Personalized Medicine

Article Title: In Vitro Investigation of Pulsed Electromagnetic Field Stimulation (PEMF) with MAGCELL ® ARTHRO on the Regulatory Expression of Soluble and Membrane-Bound Complement Factors and Inflammatory Cytokines in Immortalized Synovial Fibroblasts

doi: 10.3390/jpm14070701

Figure Lengend Snippet: Antibodies and dyes used.

Article Snippet: Mouse anti-human CD59 , Bio-Rad, Bio-Rad, Feldkirchen, Germany , MCA1054 , 1 mg/mL , 1:50.

Techniques: Concentration Assay

Journal: Journal of Personalized Medicine

Article Title: In Vitro Investigation of Pulsed Electromagnetic Field Stimulation (PEMF) with MAGCELL ® ARTHRO on the Regulatory Expression of Soluble and Membrane-Bound Complement Factors and Inflammatory Cytokines in Immortalized Synovial Fibroblasts

doi: 10.3390/jpm14070701

Figure Lengend Snippet: Graphic representation of relative gene expression of complement factors (CD55, CD59, CFH and CFI) and cytokines (IL-6 and TNFα) in synovial fibroblast cell line K4IM after PEMF stimulation [PEMF(ON)] compared to various controls. A one-day stimulation protocol was used. Ctrl = non-stimulated group, [PEMF(OFF)] = apparatus turned off, sham = apparatus turned on without electromagnetic impulse. n = 5 independent experiments. Mean with standard deviation (SD). Non-stimulated group as control has been normalized to 100. Repeated measures one-way ANOVA using Tukey’s multiple comparisons.

Article Snippet: Mouse anti-human CD59 , Bio-Rad, Bio-Rad, Feldkirchen, Germany , MCA1054 , 1 mg/mL , 1:50.

Techniques: Gene Expression, Standard Deviation, Control

Journal: Journal of Personalized Medicine

Article Title: In Vitro Investigation of Pulsed Electromagnetic Field Stimulation (PEMF) with MAGCELL ® ARTHRO on the Regulatory Expression of Soluble and Membrane-Bound Complement Factors and Inflammatory Cytokines in Immortalized Synovial Fibroblasts

doi: 10.3390/jpm14070701

Figure Lengend Snippet: Graphic representation of relative gene expression of complement factors (CD55, CD59, CFH and CFI) and cytokines (IL-6 and TNFα) in synovial fibroblast cell line K4IM after PEMF stimulation [PEMF(ON)] with and without 24 h TNFα pre-stimulation (10 ng/mL) compared to their respective control (ctrl). A one-day stimulation protocol was used. Ctrl = non-stimulation. n = 5 independent experiments. Mean with standard deviation (SD). Non-stimulated group without TNFα pre-stimulation (Ctrl/Ctrl) has been normalized to 100. Repeated measures one-way ANOVA using Tukey’s multiple comparisons (*). * = p ≤ 0.05, ** = p ≤ 0.01, *** = p ≤ 0.001, **** = p ≤ 0.0001.

Article Snippet: Mouse anti-human CD59 , Bio-Rad, Bio-Rad, Feldkirchen, Germany , MCA1054 , 1 mg/mL , 1:50.

Techniques: Gene Expression, Control, Standard Deviation

Journal: Journal of Personalized Medicine

Article Title: In Vitro Investigation of Pulsed Electromagnetic Field Stimulation (PEMF) with MAGCELL ® ARTHRO on the Regulatory Expression of Soluble and Membrane-Bound Complement Factors and Inflammatory Cytokines in Immortalized Synovial Fibroblasts

doi: 10.3390/jpm14070701

Figure Lengend Snippet: Graphic representation of relative gene expression of complement factors (CD55, CD59, CFH and CFI) and cytokines (IL-6 and TNFα) in synovial fibroblast cell line K4IM after PEMF stimulation [PEMF(ON)] for 3 days and 6 days compared to their respective control (ctrl). Ctrl = non-stimulation. n = 4 independent experiments. Mean with standard deviation (SD). Non-stimulated group (3 days) has been normalized to 100. Repeated measures one-way ANOVA using Tukey’s multiple comparisons (*) * = p ≤ 0.05, ** = p ≤ 0.01, *** = p ≤ 0.001, **** = p ≤ 0.0001.

Article Snippet: Mouse anti-human CD59 , Bio-Rad, Bio-Rad, Feldkirchen, Germany , MCA1054 , 1 mg/mL , 1:50.

Techniques: Gene Expression, Control, Standard Deviation

Journal: Journal of Personalized Medicine

Article Title: In Vitro Investigation of Pulsed Electromagnetic Field Stimulation (PEMF) with MAGCELL ® ARTHRO on the Regulatory Expression of Soluble and Membrane-Bound Complement Factors and Inflammatory Cytokines in Immortalized Synovial Fibroblasts

doi: 10.3390/jpm14070701

Figure Lengend Snippet: ( A ) Representative images of K4IM cell line after 3 days and 6 days PEMF(ON) stimulation compared to their non-stimulated counterparts, respectively (200× magnification), immunolabeled with a CD59 specific antibody and negative control of the staining. Green (Alexa Fluor 488) = CD59, blue (DAPI) = cell nuclei, gray (Phalloidin Alexa Fluor 633) = F-Actin cytoskeleton. Scale bar = 100 µm. ( B ) Graphic representation of relative CD59 protein fluorescence intensity, n = 4 independent experiments, mean with standard deviation (SD). Control (3 days) has been normalized to 100. Repeated measures one-way ANOVA using Tukey’s multiple comparisons.

Article Snippet: Mouse anti-human CD59 , Bio-Rad, Bio-Rad, Feldkirchen, Germany , MCA1054 , 1 mg/mL , 1:50.

Techniques: Immunolabeling, Negative Control, Staining, Fluorescence, Standard Deviation, Control

Journal: Journal of Personalized Medicine

Article Title: In Vitro Investigation of Pulsed Electromagnetic Field Stimulation (PEMF) with MAGCELL ® ARTHRO on the Regulatory Expression of Soluble and Membrane-Bound Complement Factors and Inflammatory Cytokines in Immortalized Synovial Fibroblasts

doi: 10.3390/jpm14070701

Figure Lengend Snippet: Antibodies and dyes used.

Article Snippet: Mouse anti-human CD59 , Bio-Rad, Bio-Rad, Feldkirchen, Germany , MCA1054 , 1 mg/mL , 1:50.

Techniques: Concentration Assay

Journal: Journal of neuroinflammation

Article Title: Complement-membrane regulatory proteins are absent from the nodes of Ranvier in the peripheral nervous system.

doi: 10.1186/s12974-023-02920-9

Figure Lengend Snippet: Fig. 2 Localization of CD59 in murine teased nerve and myelinated cultures. A CD59 (green) and ankyrin-G (AnkG, red) staining of WT teased fibers. B CD59 (green) and Caspr (red) staining of WT teased fibers. C CD59 (green) and neurofascin (Nfasc, blue) staining of WT teased fibers. D CD59 (green), myelin basic protein (MBP, red), and neurofascin (Nfasc, blue) staining of WT ICR DRG. CD59 is co-localized with MBP and localized in the internodes and paranodes. There is no CD59 localization in the nodes of Ranvier. A–C Sections taken from a 4-month-old mouse. Immunolabeling procedure handled with methanol and 0.1% triton. Scale bars 20 μm and in D 50 μm

Article Snippet: Paraffin-embedded sections of human sural biopsies were stained using IHC methods for mouse mAb against human CD59 (BIO-RAD, Hercules, CA, USA) at 1:400 dilutions, rabbit mAb against human CD55 antibody (Abcam, CB, UK) at 1:400 dilution, rabbit mAb against human CD46 (Abcam, CB, UK) at 1:1000 dilution, mouse mAb against human CD35 (Abcam, CB, UK) at 1:100 dilution, rabbit pAb against human MBP (Zymed, Waltham, MA, USA), mouse mAb against human neurofilament (NF) (Dako, Glostrup South, Denmark), and rabbit pAb against human S-100 (Dako, Glostrup South, Denmark).

Techniques: Staining, Immunolabeling

Journal: Journal of neuroinflammation

Article Title: Complement-membrane regulatory proteins are absent from the nodes of Ranvier in the peripheral nervous system.

doi: 10.1186/s12974-023-02920-9

Figure Lengend Snippet: Fig. 1 Localization of CD59 in peripheral nerve and murine sciatic nerve cross-sections. A–D CD59 (green), P0 (red) and beta dystroglycan (DG, blue) staining of murine wild-type (WT) and knock-out (KO) cross section fibers (A-D small squares). E–G CD59 (green) and myelin-associated glycoprotein (MAG, red) staining of fibers from WT mice. Staining shows that CD59 and P0, which represent compact myelin, have identical patterns in WT mice. Sections were taken from 4-month-old mice. Immunolabeling was performed with methanol and 0.1% triton. Scale bars 20 μm

Article Snippet: Paraffin-embedded sections of human sural biopsies were stained using IHC methods for mouse mAb against human CD59 (BIO-RAD, Hercules, CA, USA) at 1:400 dilutions, rabbit mAb against human CD55 antibody (Abcam, CB, UK) at 1:400 dilution, rabbit mAb against human CD46 (Abcam, CB, UK) at 1:1000 dilution, mouse mAb against human CD35 (Abcam, CB, UK) at 1:100 dilution, rabbit pAb against human MBP (Zymed, Waltham, MA, USA), mouse mAb against human neurofilament (NF) (Dako, Glostrup South, Denmark), and rabbit pAb against human S-100 (Dako, Glostrup South, Denmark).

Techniques: Staining, Knock-Out, Immunolabeling

Journal: Journal of neuroinflammation

Article Title: Complement-membrane regulatory proteins are absent from the nodes of Ranvier in the peripheral nervous system.

doi: 10.1186/s12974-023-02920-9

Figure Lengend Snippet: Fig. 3 Localization of CD59 in human sural nerve. A–C Immunohistochemical staining for CD59 (DAB, red) in sural nerves of a healthy control. Healthy control staining shows compact myelin localization (C, blue arrow heads). CD59 was also detected in endothelial blood vessels of the epineurium (A and B, red arrow heads) and endoneurium (B and C, black arrow heads), as well as the perineurium (A and B, green arrow heads). D, E Immunofluorescence staining for CD59 (red) and MBP (blue). F, G Immunofluorescence staining for CD59 (red) and CD31 (green). Scale bars: A 500 μm, B 100 μm, C–G 50 μm. Magnifications: A ×4, B, D–G and ×20, C ×40. A–C 1:400 dilution

Article Snippet: Paraffin-embedded sections of human sural biopsies were stained using IHC methods for mouse mAb against human CD59 (BIO-RAD, Hercules, CA, USA) at 1:400 dilutions, rabbit mAb against human CD55 antibody (Abcam, CB, UK) at 1:400 dilution, rabbit mAb against human CD46 (Abcam, CB, UK) at 1:1000 dilution, mouse mAb against human CD35 (Abcam, CB, UK) at 1:100 dilution, rabbit pAb against human MBP (Zymed, Waltham, MA, USA), mouse mAb against human neurofilament (NF) (Dako, Glostrup South, Denmark), and rabbit pAb against human S-100 (Dako, Glostrup South, Denmark).

Techniques: Immunohistochemical staining, Staining, Control, Immunofluorescence

Journal: Journal of neuroinflammation

Article Title: Complement-membrane regulatory proteins are absent from the nodes of Ranvier in the peripheral nervous system.

doi: 10.1186/s12974-023-02920-9

Figure Lengend Snippet: Fig. 4 Electron microscopy (EM) and immunofluorescence labeling of murine sciatic nerve. EM pictures of cross sections from WT (A–D (and CD59a-deficient (E–H) murine sciatic nerve at different ages. A and E are low-resolution toluidine blue pictures from a 6-month-old mouse. Scale bars: A and E 50 μm, B and F 10 μm, C, D, G and H 5 μm. I–L EM (higher magnification) showing accumulation of axoplasmatic organelles at an older age (18 months) in longitudinal sections from the paranodal regions (I, J, scale bars: I 2 μm, J 5 μm), and mitochondria and dense bodies in cross sections at regions with of noncompact myelin (K, scale bar = 500 nm; I–K are from CD59 KO mice). WT vs CD59 KO mice, comparison of the percentage of nodes of Ranvier with accumulation of axoplasmic organelles is shown in L (WT: n = 5, 82 nodes. KO: n = 5, 117 nodes; (p = 0.01, unpaired two-tailed student’s t-tests). M–P Immunofluorescence labeling of WT (left panels) and CD59 KO (right panels) teased fibers show normal node structure by CD59 (green) and AnkG (M, red), Caspr (N, red), neurofascin (O, Nfasc, blue), and myelin-associated glycoprotein (P, MAG, red). Sections taken from a 4-month-old mouse. Immunolabeling procedure handled with methanol and 0.1% triton. Scale bars 20 μm

Article Snippet: Paraffin-embedded sections of human sural biopsies were stained using IHC methods for mouse mAb against human CD59 (BIO-RAD, Hercules, CA, USA) at 1:400 dilutions, rabbit mAb against human CD55 antibody (Abcam, CB, UK) at 1:400 dilution, rabbit mAb against human CD46 (Abcam, CB, UK) at 1:1000 dilution, mouse mAb against human CD35 (Abcam, CB, UK) at 1:100 dilution, rabbit pAb against human MBP (Zymed, Waltham, MA, USA), mouse mAb against human neurofilament (NF) (Dako, Glostrup South, Denmark), and rabbit pAb against human S-100 (Dako, Glostrup South, Denmark).

Techniques: Electron Microscopy, Immunofluorescence, Labeling, Comparison, Two Tailed Test, Immunolabeling

Journal: Journal of neuroinflammation

Article Title: Complement-membrane regulatory proteins are absent from the nodes of Ranvier in the peripheral nervous system.

doi: 10.1186/s12974-023-02920-9

Figure Lengend Snippet: Fig. 5 CD59 patient sural nerve biopsy. A, B Immunohistochemical staining of CD59 (DAB, red) from a healthy control subject (A) and a CD59 patient (B). CD59-deficient patient staining of CD59 was completely absent in comparison to a healthy control subject where CD59 appears to localize in compact myelin. Scale bars = 50 μm, magnifications × 40, 1:400 dilution. C–H Hematoxylin and eosin (H&E) (C and D), epoxy-resin embedded semi-thin sections stained with toluidine-blue (E and F), immunohistochemical staining for MBP (G) and neurofilament (NF) (H). Staining displayed normal nerve fiber density without evidence of active axonal or myelin damage. Scale bars: C, D, G and H 50 μm; E and F 20 μm; magnifications: C, D, G and H ×40; E and F ×60. I Electron microscopy image, tissue from a CD59-deficient patient showing thin myelin sheaths relative to axonal diameter with a relatively high g-ratio, suggestive of remyelination. Scale bar 5 μm. small squares, myelin sheath lamellar structure. Scale bar 500 nm

Article Snippet: Paraffin-embedded sections of human sural biopsies were stained using IHC methods for mouse mAb against human CD59 (BIO-RAD, Hercules, CA, USA) at 1:400 dilutions, rabbit mAb against human CD55 antibody (Abcam, CB, UK) at 1:400 dilution, rabbit mAb against human CD46 (Abcam, CB, UK) at 1:1000 dilution, mouse mAb against human CD35 (Abcam, CB, UK) at 1:100 dilution, rabbit pAb against human MBP (Zymed, Waltham, MA, USA), mouse mAb against human neurofilament (NF) (Dako, Glostrup South, Denmark), and rabbit pAb against human S-100 (Dako, Glostrup South, Denmark).

Techniques: Immunohistochemical staining, Staining, Control, Comparison, Electron Microscopy

Journal: Journal of neuroinflammation

Article Title: Complement-membrane regulatory proteins are absent from the nodes of Ranvier in the peripheral nervous system.

doi: 10.1186/s12974-023-02920-9

Figure Lengend Snippet: Fig. 6 Localization of complement membrane regulatory proteins in murine teased fibers of sciatic nerve. Staining of WT teased fibers. CD55 (red), neurofascin (Nfasc, blue) and CD59 (green) (A), CD46 (red), neurofascin (Nfasc, blue) and CD59 (green) (B), Crry (red), neurofascin (Nfasc, blue) and CD59 (green) (C). Sections taken from a 4-month-old mouse. Immunolabeling procedure performed with the TSA method (A, C) and methanol and 0.1% triton (B). Scale bars 20 μm. CD55 staining was localized in Schmidt Lanterman incisures. CD46 staining was localized in the paranodes-juxtaparanodes, and Crry staining was localized in the paranodes and the internodes corresponding to compact myelin. White arrowheads indicate the nodes of Ranvier

Article Snippet: Paraffin-embedded sections of human sural biopsies were stained using IHC methods for mouse mAb against human CD59 (BIO-RAD, Hercules, CA, USA) at 1:400 dilutions, rabbit mAb against human CD55 antibody (Abcam, CB, UK) at 1:400 dilution, rabbit mAb against human CD46 (Abcam, CB, UK) at 1:1000 dilution, mouse mAb against human CD35 (Abcam, CB, UK) at 1:100 dilution, rabbit pAb against human MBP (Zymed, Waltham, MA, USA), mouse mAb against human neurofilament (NF) (Dako, Glostrup South, Denmark), and rabbit pAb against human S-100 (Dako, Glostrup South, Denmark).

Techniques: Membrane, Staining, Immunolabeling

Journal: Cells

Article Title: Complement Proteins C5/C5a, Cathepsin D and Prolactin in Chondrocytes: A Possible Crosstalk in the Pathogenesis of Osteoarthritis

doi: 10.3390/cells11071134

Figure Lengend Snippet: List of applied antibodies for the immunofluorescence staining.

Article Snippet: CD59 , Bio-Rad, Feldkirchen, Germany , Mouse-anti-human , MCA1054GA.

Techniques: Immunofluorescence, Staining