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anti jnk  (Proteintech)


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    Structured Review

    Proteintech anti jnk
    Anti Jnk, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti jnk/product/Proteintech
    Average 94 stars, based on 3 article reviews
    anti jnk - by Bioz Stars, 2026-05
    94/100 stars

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    Image Search Results


    Validation of the targeting relationship between let-7a-5p and MAPK8. (A) Schematic representation of the predicted complementary binding site between let-7a-5p and the 3′-UTR of MAPK8. (B) Relative mRNA expression level of MAPK8 in macrophages overexpressing let-7a-5p detected by RT-qPCR. (C) WB analysis of MAPK8 protein expression in macrophages overexpressing let-7a-5p. (D) Inhibitory effect of let-7a-5p on MAPK8 expression was assessed via a dual luciferase reporter assay. ( **P < 0.01, ***P < 0.001).

    Journal: Frontiers in Immunology

    Article Title: Platelet-rich plasma-derived microRNA let-7a-5p alleviates knee osteoarthritis by regulating macrophage polarization and improving inflammatory microenvironment

    doi: 10.3389/fimmu.2026.1756467

    Figure Lengend Snippet: Validation of the targeting relationship between let-7a-5p and MAPK8. (A) Schematic representation of the predicted complementary binding site between let-7a-5p and the 3′-UTR of MAPK8. (B) Relative mRNA expression level of MAPK8 in macrophages overexpressing let-7a-5p detected by RT-qPCR. (C) WB analysis of MAPK8 protein expression in macrophages overexpressing let-7a-5p. (D) Inhibitory effect of let-7a-5p on MAPK8 expression was assessed via a dual luciferase reporter assay. ( **P < 0.01, ***P < 0.001).

    Article Snippet: Subsequently, the tissue sections and cells were incubated overnight at 4°C with primary antibodies against iNOS (Proteintech 22226-1-AP), CD206 (Proteintech 18704-1-AP) and MAPK8 (Proteintech 66210-1-Ig).

    Techniques: Biomarker Discovery, Binding Assay, Expressing, Quantitative RT-PCR, Luciferase, Reporter Assay

    Effect of PRP on expression of let-7a-5p and MAPK8. (A) Relative mRNA expression levels of let-7a-5p in knee joint sections of each group detected by RT-qPCR. (B) iNOS and CD206 co-immunolabeld with MAPK8 and counter-stained with DAPI in synovial tissues (Scale bar: 50 μm). (C, D) Quantification of iNOS and CD206 expression co-localized with MAPK8. ( **P < 0.01).

    Journal: Frontiers in Immunology

    Article Title: Platelet-rich plasma-derived microRNA let-7a-5p alleviates knee osteoarthritis by regulating macrophage polarization and improving inflammatory microenvironment

    doi: 10.3389/fimmu.2026.1756467

    Figure Lengend Snippet: Effect of PRP on expression of let-7a-5p and MAPK8. (A) Relative mRNA expression levels of let-7a-5p in knee joint sections of each group detected by RT-qPCR. (B) iNOS and CD206 co-immunolabeld with MAPK8 and counter-stained with DAPI in synovial tissues (Scale bar: 50 μm). (C, D) Quantification of iNOS and CD206 expression co-localized with MAPK8. ( **P < 0.01).

    Article Snippet: Subsequently, the tissue sections and cells were incubated overnight at 4°C with primary antibodies against iNOS (Proteintech 22226-1-AP), CD206 (Proteintech 18704-1-AP) and MAPK8 (Proteintech 66210-1-Ig).

    Techniques: Expressing, Quantitative RT-PCR, Staining

    The let-7a-5p/MAPK8 axis regulates macrophage polarization and inflammatory cytokine release in vitro . (A) IF staining showing expression of iNOS and CD206 in macrophages (Scale bar: 50 μm). (B) Quantification of iNOS-positive cell rate in transfected macrophages. (C) Quantification of CD206-positive cell rate in transfected macrophages. (D-G) Relative mRNA expression levels of pro-inflammatory cytokine (IL-1β and TNF-α) and anti-inflammatory cytokine (IL-4 and IL-10) in transfected macrophages detected by RT-qPCR. ( *P < 0.05, **P < 0.01, ns no significance).

    Journal: Frontiers in Immunology

    Article Title: Platelet-rich plasma-derived microRNA let-7a-5p alleviates knee osteoarthritis by regulating macrophage polarization and improving inflammatory microenvironment

    doi: 10.3389/fimmu.2026.1756467

    Figure Lengend Snippet: The let-7a-5p/MAPK8 axis regulates macrophage polarization and inflammatory cytokine release in vitro . (A) IF staining showing expression of iNOS and CD206 in macrophages (Scale bar: 50 μm). (B) Quantification of iNOS-positive cell rate in transfected macrophages. (C) Quantification of CD206-positive cell rate in transfected macrophages. (D-G) Relative mRNA expression levels of pro-inflammatory cytokine (IL-1β and TNF-α) and anti-inflammatory cytokine (IL-4 and IL-10) in transfected macrophages detected by RT-qPCR. ( *P < 0.05, **P < 0.01, ns no significance).

    Article Snippet: Subsequently, the tissue sections and cells were incubated overnight at 4°C with primary antibodies against iNOS (Proteintech 22226-1-AP), CD206 (Proteintech 18704-1-AP) and MAPK8 (Proteintech 66210-1-Ig).

    Techniques: In Vitro, Staining, Expressing, Transfection, Quantitative RT-PCR

    Comparison of MAP2K7, MAPK9, and MAPK8 gene expression and histological grade of CRC. * multiplication sign.

    Journal: International Journal of Molecular Sciences

    Article Title: Expression and Clinical Significance of MAPK8 , MAPK9 , MAP2K4 , and MAP2K7 Genes in Colorectal Cancer

    doi: 10.3390/ijms27010100

    Figure Lengend Snippet: Comparison of MAP2K7, MAPK9, and MAPK8 gene expression and histological grade of CRC. * multiplication sign.

    Article Snippet: Assay IDs for molecular probes: Hs00387426_m1—MAP2K4; Hs01547883_g1—MAP2K7; Hs01548508_m1—MAPK8; Hs01558224_m1—MAPK9; Hs02786624_g1—GAPDH; and Hs01060665_g1—ACTB.

    Techniques: Comparison, Gene Expression

    Comparison between MAPK9 and MAPK8 expression and the nodal metastasis of CRC.

    Journal: International Journal of Molecular Sciences

    Article Title: Expression and Clinical Significance of MAPK8 , MAPK9 , MAP2K4 , and MAP2K7 Genes in Colorectal Cancer

    doi: 10.3390/ijms27010100

    Figure Lengend Snippet: Comparison between MAPK9 and MAPK8 expression and the nodal metastasis of CRC.

    Article Snippet: Assay IDs for molecular probes: Hs00387426_m1—MAP2K4; Hs01547883_g1—MAP2K7; Hs01548508_m1—MAPK8; Hs01558224_m1—MAPK9; Hs02786624_g1—GAPDH; and Hs01060665_g1—ACTB.

    Techniques: Comparison, Expressing

    Comparison of MAPK8 , MAP2K7 , and MAP2K4 gene expression between colon tumor tissue and normal colon tissue.

    Journal: International Journal of Molecular Sciences

    Article Title: Expression and Clinical Significance of MAPK8 , MAPK9 , MAP2K4 , and MAP2K7 Genes in Colorectal Cancer

    doi: 10.3390/ijms27010100

    Figure Lengend Snippet: Comparison of MAPK8 , MAP2K7 , and MAP2K4 gene expression between colon tumor tissue and normal colon tissue.

    Article Snippet: Assay IDs for molecular probes: Hs00387426_m1—MAP2K4; Hs01547883_g1—MAP2K7; Hs01548508_m1—MAPK8; Hs01558224_m1—MAPK9; Hs02786624_g1—GAPDH; and Hs01060665_g1—ACTB.

    Techniques: Comparison, Gene Expression

    Comparison of MAPK8 , MAP2K4 , and MAP2K7 gene expression with various clinicopathological features.

    Journal: International Journal of Molecular Sciences

    Article Title: Expression and Clinical Significance of MAPK8 , MAPK9 , MAP2K4 , and MAP2K7 Genes in Colorectal Cancer

    doi: 10.3390/ijms27010100

    Figure Lengend Snippet: Comparison of MAPK8 , MAP2K4 , and MAP2K7 gene expression with various clinicopathological features.

    Article Snippet: Assay IDs for molecular probes: Hs00387426_m1—MAP2K4; Hs01547883_g1—MAP2K7; Hs01548508_m1—MAPK8; Hs01558224_m1—MAPK9; Hs02786624_g1—GAPDH; and Hs01060665_g1—ACTB.

    Techniques: Comparison, Gene Expression

    Kaplan–Meier curves representing the survival rate of patients with colorectal cancer in relation to MAPK8 , MAPK9 , MAP2K4 , and MAP2K7 expression.

    Journal: International Journal of Molecular Sciences

    Article Title: Expression and Clinical Significance of MAPK8 , MAPK9 , MAP2K4 , and MAP2K7 Genes in Colorectal Cancer

    doi: 10.3390/ijms27010100

    Figure Lengend Snippet: Kaplan–Meier curves representing the survival rate of patients with colorectal cancer in relation to MAPK8 , MAPK9 , MAP2K4 , and MAP2K7 expression.

    Article Snippet: Assay IDs for molecular probes: Hs00387426_m1—MAP2K4; Hs01547883_g1—MAP2K7; Hs01548508_m1—MAPK8; Hs01558224_m1—MAPK9; Hs02786624_g1—GAPDH; and Hs01060665_g1—ACTB.

    Techniques: Expressing

    Differences in the expression of MAPK8, MAPK9, MAP2K4, and MAP2K7 genes in colorectal cancer tissue according to CMS. p -values were calculated using one-way ANOVA.

    Journal: International Journal of Molecular Sciences

    Article Title: Expression and Clinical Significance of MAPK8 , MAPK9 , MAP2K4 , and MAP2K7 Genes in Colorectal Cancer

    doi: 10.3390/ijms27010100

    Figure Lengend Snippet: Differences in the expression of MAPK8, MAPK9, MAP2K4, and MAP2K7 genes in colorectal cancer tissue according to CMS. p -values were calculated using one-way ANOVA.

    Article Snippet: Assay IDs for molecular probes: Hs00387426_m1—MAP2K4; Hs01547883_g1—MAP2K7; Hs01548508_m1—MAPK8; Hs01558224_m1—MAPK9; Hs02786624_g1—GAPDH; and Hs01060665_g1—ACTB.

    Techniques: Expressing