Virus:Article Title: A circuit of mossy cells controls the efficacy of memory retrieval by Gria2I inhibition of Gria2.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse-anti-Calb2 Swant Cat#6B3; RRID: AB_10000320 Rabbit-anti-Calb1 Swant Cat#CB38; RRID: AB_10000340 Rat-anti-somatostatin Millipore Cat# MAB354; RRID: AB_2255365 Rabbit-anti-DCX Abcam Cat#ab18723; RRID: AB_732011 Rat-anti-somatostatin Abcam Cat# ab30788; RRID: AB_778010 Rabbit-anti-c-fos sysy Cat#226003; RRID: AB_2231974 Rabbit-anti-GluR2/3 Millipore Cat# AB1506; RRID: AB_90710 Mouse anti-GAD67 Millipore Cat# MAB5406; RRID: AB_2278725 Rabbit-anti-parvalbumin Swant Cat# PV27; RRID: AB_2231173 Rabbit-anti- REST/NRSE Abcam Cat#ab21635; RRID: AB_777678 Rabbit-anti-Gria1 Sigma-Aldrich Cat# Ab-863; RRID: AB_10621299 Mouse-anti-Gria2 Millipore Cat# MABN103; RRID: AB_10850324 Rabbit-anti-tubulin Abcam Cat# ab18251; RRID: AB_2210057 Alexa Fluor 647 donkey anti-rabbit Thermo Fisher Cat#A32795; RRID: AB_2762835 Alexa Fluor 488 donkey anti-mouse Thermo Fisher Cat#A-21202; RRID: AB_141607 Alexa Fluor 488 donkey anti-rabbit Thermo Fisher Cat#A-21206; RRID: AB_2535792 Alexa Fluor 594 donkey anti-rat Thermo Fisher Cat#A-21209; RRID: AB_2535795 Alexa Fluor 546 donkey anti-mouse Thermo Fisher Cat#A10036; RRID: AB_2534012 Alexa Fluor 546 donkey anti-rabbit Thermo Fisher Cat#A10040; RRID: AB_2534016 Alexa Fluor 488 donkey anti-rat Thermo Fisher Cat#A-21208; RRID: AB_141709 Alexa Fluor 405 goat anti-rabbit Thermo Fisher Cat#A-31556; RRID: AB_221605 Alexa Fluor 647 donkey anti-mouse Thermo Fisher Cat#A-31571; RRID: AB_162542 Alexa Fluor 647 goat anti-rat Thermo Fisher Cat#A-21247; RRID: AB_141778 Bacterial and virus strains AAV-CaMKIIa-DIO-GFP This paper Taitool (Shanghai) AAV-CaMKIIa-DIO-Kir2.1tdT This paper Taitool (Shanghai) AAV-CaMKIIa-DIO- Kir2.1tdT-IRESChR2GFP This paper Taitool (Shanghai) AAV-EF1a-fDIO-GFP Taiting Biotechnology (Shanghai) S-0253 AAV-EF1a-fDIO-ChR2GFP Taiting Biotechnology (Shanghai) S-0129 AAV-EF1a-fDIO-Gria2GFP This paper Taitool (Shanghai) AAV-EF1a-fDIO-Gria2QGFP This paper Taitool (Shanghai) AAV-EF1a-fDIO-Gria2I-IGFP This paper Taitool (Shanghai) AAV-EF1a-fDIO-Gria2I-SIGFP This paper Taitool (Shanghai) AAV-EF1a-DIO-TVA/G/GFP BrainVTA (Wuhan) PT-0062 DRV (Sad B19 strain) BrainVTA (Wuhan) R01002 AAV-CaMKIIa-DIO-TKGFP Zeng et al., 2017 N/A H129DTKtdT Zeng et al., 2017 N/A Experimental models: organisms/strains Mouse: Calb2-CRE The Jackson Laboratory Stock No.010774 Mouse: SST-CRE The Jackson Laboratory Stock No.018973 Mouse: PV-CRE The Jackson Laboratory Stock No.008069 Mouse: SST-FLP This paper Beijing Biocytogen Co. (Continued on next page) Cell Reports 34, 108741, February 16, 2021 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms Offline Sorter Plexon Version: 3.3.5 MATLAB MathWorks, Inc.
Article Title: Differential behavior-related activity of distinct hippocampal interneuron types during odor-associated spatial navigation.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Parvalbumin (PV), 1:2000, host: guinea pig Synaptic Systems Cat. No. 195004; RRID:AB_2156476 SATB1, 1:500, host: rabbit Abcam Cat. No. ab70004; RRID:AB_1270545 NPY, 1:5000, host: rabbit Immunostar Cat. No. 22940; RRID:AB_2307354 SOM, 1:500, host. rat Millipore Cat. No. MAB354; RRID:AB_2255365 CB1R, 1:1000, host: rabbit Dr. M. Watanabe N/A VIP, 1:10000, host: rabbit Dr. T. Görcs N/A CCK, 1:5000, host: mouse Abcam Cat. No. ab37274; RRID:AB_726010 Ch2R2, 1:10000, host: mouse Progen Cat. No. 651180; RRID:AB_2892521 Secondary antibodies Jackson Laboratories N/A Bacterial and virus strains AAV2/1-mDlx-ChR2 Dimidschstein et al.96 https://www.addgene.or/83898/ Chemicals, peptides, and recombinant proteins Neurobiotin Tracer Vector Laboratories Cat. No. SP-1120; RRID:AB_2313575 Critical commercial assays Vectastain ABC kit Vector Laboratories Cat. No. 6100; RRID:AB_2336819 VECTASHIELD Antifade Mounting Medium Vector Laboratories Cat. No. H-1000; RRID:AB_2336789 Deposited data Original code and datasets Zenodo data: https://doi.org/10.5281/zenodo.7908539 N/A Experimental models: Organisms/strains C57BL/6J Center for Brain Research breeding by Dr. Petra Scholze Software and algorithms MATLAB MathWorks Version R2014a; https://uk.mathworks.com/ products/matlab.html Spike2 Cambridge Electronic Design Limited (CED) Spike2 version 7 klusta Rossant et al.97 https://github.com/kwikteam/klusta Other Jetball TFT-System PhenoSys https://www.phenosys.com/products/ virtual-reality/jetball-tft/ Silicon probe: Buz16 NeuroNexus http://neuronexus.com/ ..
Bioprocessing:Article Title: A circuit of mossy cells controls the efficacy of memory retrieval by Gria2I inhibition of Gria2.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse-anti-Calb2 Swant Cat#6B3; RRID: AB_10000320 Rabbit-anti-Calb1 Swant Cat#CB38; RRID: AB_10000340 Rat-anti-somatostatin Millipore Cat# MAB354; RRID: AB_2255365 Rabbit-anti-DCX Abcam Cat#ab18723; RRID: AB_732011 Rat-anti-somatostatin Abcam Cat# ab30788; RRID: AB_778010 Rabbit-anti-c-fos sysy Cat#226003; RRID: AB_2231974 Rabbit-anti-GluR2/3 Millipore Cat# AB1506; RRID: AB_90710 Mouse anti-GAD67 Millipore Cat# MAB5406; RRID: AB_2278725 Rabbit-anti-parvalbumin Swant Cat# PV27; RRID: AB_2231173 Rabbit-anti- REST/NRSE Abcam Cat#ab21635; RRID: AB_777678 Rabbit-anti-Gria1 Sigma-Aldrich Cat# Ab-863; RRID: AB_10621299 Mouse-anti-Gria2 Millipore Cat# MABN103; RRID: AB_10850324 Rabbit-anti-tubulin Abcam Cat# ab18251; RRID: AB_2210057 Alexa Fluor 647 donkey anti-rabbit Thermo Fisher Cat#A32795; RRID: AB_2762835 Alexa Fluor 488 donkey anti-mouse Thermo Fisher Cat#A-21202; RRID: AB_141607 Alexa Fluor 488 donkey anti-rabbit Thermo Fisher Cat#A-21206; RRID: AB_2535792 Alexa Fluor 594 donkey anti-rat Thermo Fisher Cat#A-21209; RRID: AB_2535795 Alexa Fluor 546 donkey anti-mouse Thermo Fisher Cat#A10036; RRID: AB_2534012 Alexa Fluor 546 donkey anti-rabbit Thermo Fisher Cat#A10040; RRID: AB_2534016 Alexa Fluor 488 donkey anti-rat Thermo Fisher Cat#A-21208; RRID: AB_141709 Alexa Fluor 405 goat anti-rabbit Thermo Fisher Cat#A-31556; RRID: AB_221605 Alexa Fluor 647 donkey anti-mouse Thermo Fisher Cat#A-31571; RRID: AB_162542 Alexa Fluor 647 goat anti-rat Thermo Fisher Cat#A-21247; RRID: AB_141778 Bacterial and virus strains AAV-CaMKIIa-DIO-GFP This paper Taitool (Shanghai) AAV-CaMKIIa-DIO-Kir2.1tdT This paper Taitool (Shanghai) AAV-CaMKIIa-DIO- Kir2.1tdT-IRESChR2GFP This paper Taitool (Shanghai) AAV-EF1a-fDIO-GFP Taiting Biotechnology (Shanghai) S-0253 AAV-EF1a-fDIO-ChR2GFP Taiting Biotechnology (Shanghai) S-0129 AAV-EF1a-fDIO-Gria2GFP This paper Taitool (Shanghai) AAV-EF1a-fDIO-Gria2QGFP This paper Taitool (Shanghai) AAV-EF1a-fDIO-Gria2I-IGFP This paper Taitool (Shanghai) AAV-EF1a-fDIO-Gria2I-SIGFP This paper Taitool (Shanghai) AAV-EF1a-DIO-TVA/G/GFP BrainVTA (Wuhan) PT-0062 DRV (Sad B19 strain) BrainVTA (Wuhan) R01002 AAV-CaMKIIa-DIO-TKGFP Zeng et al., 2017 N/A H129DTKtdT Zeng et al., 2017 N/A Experimental models: organisms/strains Mouse: Calb2-CRE The Jackson Laboratory Stock No.010774 Mouse: SST-CRE The Jackson Laboratory Stock No.018973 Mouse: PV-CRE The Jackson Laboratory Stock No.008069 Mouse: SST-FLP This paper Beijing Biocytogen Co. (Continued on next page) Cell Reports 34, 108741, February 16, 2021 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms Offline Sorter Plexon Version: 3.3.5 MATLAB MathWorks, Inc.
other:
Recombinant:Article Title: Differential behavior-related activity of distinct hippocampal interneuron types during odor-associated spatial navigation.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Parvalbumin (PV), 1:2000, host: guinea pig Synaptic Systems Cat. No. 195004; RRID:AB_2156476 SATB1, 1:500, host: rabbit Abcam Cat. No. ab70004; RRID:AB_1270545 NPY, 1:5000, host: rabbit Immunostar Cat. No. 22940; RRID:AB_2307354 SOM, 1:500, host. rat Millipore Cat. No. MAB354; RRID:AB_2255365 CB1R, 1:1000, host: rabbit Dr. M. Watanabe N/A VIP, 1:10000, host: rabbit Dr. T. Görcs N/A CCK, 1:5000, host: mouse Abcam Cat. No. ab37274; RRID:AB_726010 Ch2R2, 1:10000, host: mouse Progen Cat. No. 651180; RRID:AB_2892521 Secondary antibodies Jackson Laboratories N/A Bacterial and virus strains AAV2/1-mDlx-ChR2 Dimidschstein et al.96 https://www.addgene.or/83898/ Chemicals, peptides, and recombinant proteins Neurobiotin Tracer Vector Laboratories Cat. No. SP-1120; RRID:AB_2313575 Critical commercial assays Vectastain ABC kit Vector Laboratories Cat. No. 6100; RRID:AB_2336819 VECTASHIELD Antifade Mounting Medium Vector Laboratories Cat. No. H-1000; RRID:AB_2336789 Deposited data Original code and datasets Zenodo data: https://doi.org/10.5281/zenodo.7908539 N/A Experimental models: Organisms/strains C57BL/6J Center for Brain Research breeding by Dr. Petra Scholze Software and algorithms MATLAB MathWorks Version R2014a; https://uk.mathworks.com/ products/matlab.html Spike2 Cambridge Electronic Design Limited (CED) Spike2 version 7 klusta Rossant et al.97 https://github.com/kwikteam/klusta Other Jetball TFT-System PhenoSys https://www.phenosys.com/products/ virtual-reality/jetball-tft/ Silicon probe: Buz16 NeuroNexus http://neuronexus.com/ ..
Article Title: Transcriptional maintenance of cortical somatostatin interneuron subtype identity during migration.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken anti-GFP Abcam Cat#: Ab13970; RRID: AB_300798 Guinea pig anti-Sox6 Michael Wegner lab25 RRID:AB_2891329 Rat anti-Sst Millipore Cat#: MAB354; RRID: 2255365 Chemicals, peptides, and recombinant proteins DRAQ7 Dye Invitrogen Cat#: D15106 RNAscope Probe-Mm-Calb2 Advanced Cell Diagnostics Cat#: 485811-C2 RNAscope Probe-Mm-Chrna2 Advanced Cell Diagnostics Cat#: 416881-C2 Cat#: 416881-C3 RNAscope Probe-Mm-Chodl Advanced Cell Diagnostics Cat#: 450211 Cat#: 450211-C3 RNAscope Probe-Mm-Crh Advanced Cell Diagnostics Cat#: 316091-C2 RNAscope Probe-Mm-Hpse N/A Cat#: 412251 RNAscope Probe-Mm-Rasl11a-E2-E4 Advanced Cell Diagnostics Cat#: 471741 RNAscope Probe-Mm-Sst Advanced Cell Diagnostics Cat#: 404631-C2 Cat#: 404631-C3 RNAscope Probe-Mm-Tac1 Advanced Cell Diagnostics Cat#: 410351-C3 RNAscope Probe-Mm-Th Advanced Cell Diagnostics Cat#: 317621-C2 ROTI -Histofix 4% phosphate-buffered formaldehyde solution Carl Roth Cat#: P087 TSA plus Cyanine 3 Akoya Biosciences Cat#: NEL744001KT TSA plus Cyanine 5 Akoya Biosciences Cat#: NEL745001KT Critical commercial assays Papain dissociation system Worthington Cat#: LK003150 RNAscope Fluorescent Multiplex Advanced Cell Diagnostics Cat#: 320850 RNAscope Fluorescent Multiplex v2 Advanced Cell Diagnostics Cat#: 323110 RNAscope H2O2 and Protease reagents Advanced Cell Diagnostics Cat#: 322381 Deposited data Raw and pre-processed scRNA-seq data This study GEO: GSE233791 Reference adult Sst+ SMART-seq dataset Tasic et al.3 GEO: GSE115746 Experimental models: Organisms/strains Mouse: CD1 wt (RjOrl:SWISS) Janvier labs MGI: 6430821 Mouse: SstCre: Ssttm2.1(cre)Zjh/J The Jackson Laboratory Stock No: 013044 Mouse: RCEeGFP: Gt(ROSA)26Sortm1.1(CAG-EGFP)Fsh/Mmjax The Jackson Laboratory Stock No: 032037 Mouse: Sox6fl/+: Sox6tm2.1Vlf Véronique Lefebvre lab33 MGI: 3641204 Mouse: Sox6fl/-: Sox6tm2.2Vlf Véronique Lefebvre lab33 MGI: 3641205 Mouse: Ai9: B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J The Jackson Laboratory Stock No: 007909 Mouse: Dlx1/2CreERT2: Tg(I12b-cre/ERT2,-ALPP)37Fsh/J The Jackson Laboratory Stock No: 014600 Mouse: PvalbCre: B6;129P2-Pvalbtm1(cre)Arbr/J The Jackson Laboratory Stock No: 008069 Software and algorithms BackSPIN69 https://github.com/linnarsson-lab/BackSPIN N/A Clampfit Molecular Devices version 10.7 DESeq239 https://github.com/mikelove/DESeq2 version 1.34.0 Emmeans https://github.com/cran/emmeans version 1.7.0 ImageJ https://imagej.net/ij/index.html Version 1.53j (Continued on next page) e1 Neuron 111, 3590–3603.e1–e5, November 15, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER R https://www.r-project.org/ version 4.1.1 RStudio https://www.rstudio.com version 1.4.1717 Samtools70 http://www.htslib.org/ version 1.10 Seurat46 https://satijalab.org/seurat/index.html version 4.0.5 SPSS IBM version 17
Software:Article Title: Differential behavior-related activity of distinct hippocampal interneuron types during odor-associated spatial navigation.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Parvalbumin (PV), 1:2000, host: guinea pig Synaptic Systems Cat. No. 195004; RRID:AB_2156476 SATB1, 1:500, host: rabbit Abcam Cat. No. ab70004; RRID:AB_1270545 NPY, 1:5000, host: rabbit Immunostar Cat. No. 22940; RRID:AB_2307354 SOM, 1:500, host. rat Millipore Cat. No. MAB354; RRID:AB_2255365 CB1R, 1:1000, host: rabbit Dr. M. Watanabe N/A VIP, 1:10000, host: rabbit Dr. T. Görcs N/A CCK, 1:5000, host: mouse Abcam Cat. No. ab37274; RRID:AB_726010 Ch2R2, 1:10000, host: mouse Progen Cat. No. 651180; RRID:AB_2892521 Secondary antibodies Jackson Laboratories N/A Bacterial and virus strains AAV2/1-mDlx-ChR2 Dimidschstein et al.96 https://www.addgene.or/83898/ Chemicals, peptides, and recombinant proteins Neurobiotin Tracer Vector Laboratories Cat. No. SP-1120; RRID:AB_2313575 Critical commercial assays Vectastain ABC kit Vector Laboratories Cat. No. 6100; RRID:AB_2336819 VECTASHIELD Antifade Mounting Medium Vector Laboratories Cat. No. H-1000; RRID:AB_2336789 Deposited data Original code and datasets Zenodo data: https://doi.org/10.5281/zenodo.7908539 N/A Experimental models: Organisms/strains C57BL/6J Center for Brain Research breeding by Dr. Petra Scholze Software and algorithms MATLAB MathWorks Version R2014a; https://uk.mathworks.com/ products/matlab.html Spike2 Cambridge Electronic Design Limited (CED) Spike2 version 7 klusta Rossant et al.97 https://github.com/kwikteam/klusta Other Jetball TFT-System PhenoSys https://www.phenosys.com/products/ virtual-reality/jetball-tft/ Silicon probe: Buz16 NeuroNexus http://neuronexus.com/ ..
Article Title: Transcriptional maintenance of cortical somatostatin interneuron subtype identity during migration.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken anti-GFP Abcam Cat#: Ab13970; RRID: AB_300798 Guinea pig anti-Sox6 Michael Wegner lab25 RRID:AB_2891329 Rat anti-Sst Millipore Cat#: MAB354; RRID: 2255365 Chemicals, peptides, and recombinant proteins DRAQ7 Dye Invitrogen Cat#: D15106 RNAscope Probe-Mm-Calb2 Advanced Cell Diagnostics Cat#: 485811-C2 RNAscope Probe-Mm-Chrna2 Advanced Cell Diagnostics Cat#: 416881-C2 Cat#: 416881-C3 RNAscope Probe-Mm-Chodl Advanced Cell Diagnostics Cat#: 450211 Cat#: 450211-C3 RNAscope Probe-Mm-Crh Advanced Cell Diagnostics Cat#: 316091-C2 RNAscope Probe-Mm-Hpse N/A Cat#: 412251 RNAscope Probe-Mm-Rasl11a-E2-E4 Advanced Cell Diagnostics Cat#: 471741 RNAscope Probe-Mm-Sst Advanced Cell Diagnostics Cat#: 404631-C2 Cat#: 404631-C3 RNAscope Probe-Mm-Tac1 Advanced Cell Diagnostics Cat#: 410351-C3 RNAscope Probe-Mm-Th Advanced Cell Diagnostics Cat#: 317621-C2 ROTI -Histofix 4% phosphate-buffered formaldehyde solution Carl Roth Cat#: P087 TSA plus Cyanine 3 Akoya Biosciences Cat#: NEL744001KT TSA plus Cyanine 5 Akoya Biosciences Cat#: NEL745001KT Critical commercial assays Papain dissociation system Worthington Cat#: LK003150 RNAscope Fluorescent Multiplex Advanced Cell Diagnostics Cat#: 320850 RNAscope Fluorescent Multiplex v2 Advanced Cell Diagnostics Cat#: 323110 RNAscope H2O2 and Protease reagents Advanced Cell Diagnostics Cat#: 322381 Deposited data Raw and pre-processed scRNA-seq data This study GEO: GSE233791 Reference adult Sst+ SMART-seq dataset Tasic et al.3 GEO: GSE115746 Experimental models: Organisms/strains Mouse: CD1 wt (RjOrl:SWISS) Janvier labs MGI: 6430821 Mouse: SstCre: Ssttm2.1(cre)Zjh/J The Jackson Laboratory Stock No: 013044 Mouse: RCEeGFP: Gt(ROSA)26Sortm1.1(CAG-EGFP)Fsh/Mmjax The Jackson Laboratory Stock No: 032037 Mouse: Sox6fl/+: Sox6tm2.1Vlf Véronique Lefebvre lab33 MGI: 3641204 Mouse: Sox6fl/-: Sox6tm2.2Vlf Véronique Lefebvre lab33 MGI: 3641205 Mouse: Ai9: B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J The Jackson Laboratory Stock No: 007909 Mouse: Dlx1/2CreERT2: Tg(I12b-cre/ERT2,-ALPP)37Fsh/J The Jackson Laboratory Stock No: 014600 Mouse: PvalbCre: B6;129P2-Pvalbtm1(cre)Arbr/J The Jackson Laboratory Stock No: 008069 Software and algorithms BackSPIN69 https://github.com/linnarsson-lab/BackSPIN N/A Clampfit Molecular Devices version 10.7 DESeq239 https://github.com/mikelove/DESeq2 version 1.34.0 Emmeans https://github.com/cran/emmeans version 1.7.0 ImageJ https://imagej.net/ij/index.html Version 1.53j (Continued on next page) e1 Neuron 111, 3590–3603.e1–e5, November 15, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER R https://www.r-project.org/ version 4.1.1 RStudio https://www.rstudio.com version 1.4.1717 Samtools70 http://www.htslib.org/ version 1.10 Seurat46 https://satijalab.org/seurat/index.html version 4.0.5 SPSS IBM version 17
RNAscope:Article Title: Transcriptional maintenance of cortical somatostatin interneuron subtype identity during migration.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken anti-GFP Abcam Cat#: Ab13970; RRID: AB_300798 Guinea pig anti-Sox6 Michael Wegner lab25 RRID:AB_2891329 Rat anti-Sst Millipore Cat#: MAB354; RRID: 2255365 Chemicals, peptides, and recombinant proteins DRAQ7 Dye Invitrogen Cat#: D15106 RNAscope Probe-Mm-Calb2 Advanced Cell Diagnostics Cat#: 485811-C2 RNAscope Probe-Mm-Chrna2 Advanced Cell Diagnostics Cat#: 416881-C2 Cat#: 416881-C3 RNAscope Probe-Mm-Chodl Advanced Cell Diagnostics Cat#: 450211 Cat#: 450211-C3 RNAscope Probe-Mm-Crh Advanced Cell Diagnostics Cat#: 316091-C2 RNAscope Probe-Mm-Hpse N/A Cat#: 412251 RNAscope Probe-Mm-Rasl11a-E2-E4 Advanced Cell Diagnostics Cat#: 471741 RNAscope Probe-Mm-Sst Advanced Cell Diagnostics Cat#: 404631-C2 Cat#: 404631-C3 RNAscope Probe-Mm-Tac1 Advanced Cell Diagnostics Cat#: 410351-C3 RNAscope Probe-Mm-Th Advanced Cell Diagnostics Cat#: 317621-C2 ROTI -Histofix 4% phosphate-buffered formaldehyde solution Carl Roth Cat#: P087 TSA plus Cyanine 3 Akoya Biosciences Cat#: NEL744001KT TSA plus Cyanine 5 Akoya Biosciences Cat#: NEL745001KT Critical commercial assays Papain dissociation system Worthington Cat#: LK003150 RNAscope Fluorescent Multiplex Advanced Cell Diagnostics Cat#: 320850 RNAscope Fluorescent Multiplex v2 Advanced Cell Diagnostics Cat#: 323110 RNAscope H2O2 and Protease reagents Advanced Cell Diagnostics Cat#: 322381 Deposited data Raw and pre-processed scRNA-seq data This study GEO: GSE233791 Reference adult Sst+ SMART-seq dataset Tasic et al.3 GEO: GSE115746 Experimental models: Organisms/strains Mouse: CD1 wt (RjOrl:SWISS) Janvier labs MGI: 6430821 Mouse: SstCre: Ssttm2.1(cre)Zjh/J The Jackson Laboratory Stock No: 013044 Mouse: RCEeGFP: Gt(ROSA)26Sortm1.1(CAG-EGFP)Fsh/Mmjax The Jackson Laboratory Stock No: 032037 Mouse: Sox6fl/+: Sox6tm2.1Vlf Véronique Lefebvre lab33 MGI: 3641204 Mouse: Sox6fl/-: Sox6tm2.2Vlf Véronique Lefebvre lab33 MGI: 3641205 Mouse: Ai9: B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J The Jackson Laboratory Stock No: 007909 Mouse: Dlx1/2CreERT2: Tg(I12b-cre/ERT2,-ALPP)37Fsh/J The Jackson Laboratory Stock No: 014600 Mouse: PvalbCre: B6;129P2-Pvalbtm1(cre)Arbr/J The Jackson Laboratory Stock No: 008069 Software and algorithms BackSPIN69 https://github.com/linnarsson-lab/BackSPIN N/A Clampfit Molecular Devices version 10.7 DESeq239 https://github.com/mikelove/DESeq2 version 1.34.0 Emmeans https://github.com/cran/emmeans version 1.7.0 ImageJ https://imagej.net/ij/index.html Version 1.53j (Continued on next page) e1 Neuron 111, 3590–3603.e1–e5, November 15, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER R https://www.r-project.org/ version 4.1.1 RStudio https://www.rstudio.com version 1.4.1717 Samtools70 http://www.htslib.org/ version 1.10 Seurat46 https://satijalab.org/seurat/index.html version 4.0.5 SPSS IBM version 17
Multiplex Assay:Article Title: Transcriptional maintenance of cortical somatostatin interneuron subtype identity during migration.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken anti-GFP Abcam Cat#: Ab13970; RRID: AB_300798 Guinea pig anti-Sox6 Michael Wegner lab25 RRID:AB_2891329 Rat anti-Sst Millipore Cat#: MAB354; RRID: 2255365 Chemicals, peptides, and recombinant proteins DRAQ7 Dye Invitrogen Cat#: D15106 RNAscope Probe-Mm-Calb2 Advanced Cell Diagnostics Cat#: 485811-C2 RNAscope Probe-Mm-Chrna2 Advanced Cell Diagnostics Cat#: 416881-C2 Cat#: 416881-C3 RNAscope Probe-Mm-Chodl Advanced Cell Diagnostics Cat#: 450211 Cat#: 450211-C3 RNAscope Probe-Mm-Crh Advanced Cell Diagnostics Cat#: 316091-C2 RNAscope Probe-Mm-Hpse N/A Cat#: 412251 RNAscope Probe-Mm-Rasl11a-E2-E4 Advanced Cell Diagnostics Cat#: 471741 RNAscope Probe-Mm-Sst Advanced Cell Diagnostics Cat#: 404631-C2 Cat#: 404631-C3 RNAscope Probe-Mm-Tac1 Advanced Cell Diagnostics Cat#: 410351-C3 RNAscope Probe-Mm-Th Advanced Cell Diagnostics Cat#: 317621-C2 ROTI -Histofix 4% phosphate-buffered formaldehyde solution Carl Roth Cat#: P087 TSA plus Cyanine 3 Akoya Biosciences Cat#: NEL744001KT TSA plus Cyanine 5 Akoya Biosciences Cat#: NEL745001KT Critical commercial assays Papain dissociation system Worthington Cat#: LK003150 RNAscope Fluorescent Multiplex Advanced Cell Diagnostics Cat#: 320850 RNAscope Fluorescent Multiplex v2 Advanced Cell Diagnostics Cat#: 323110 RNAscope H2O2 and Protease reagents Advanced Cell Diagnostics Cat#: 322381 Deposited data Raw and pre-processed scRNA-seq data This study GEO: GSE233791 Reference adult Sst+ SMART-seq dataset Tasic et al.3 GEO: GSE115746 Experimental models: Organisms/strains Mouse: CD1 wt (RjOrl:SWISS) Janvier labs MGI: 6430821 Mouse: SstCre: Ssttm2.1(cre)Zjh/J The Jackson Laboratory Stock No: 013044 Mouse: RCEeGFP: Gt(ROSA)26Sortm1.1(CAG-EGFP)Fsh/Mmjax The Jackson Laboratory Stock No: 032037 Mouse: Sox6fl/+: Sox6tm2.1Vlf Véronique Lefebvre lab33 MGI: 3641204 Mouse: Sox6fl/-: Sox6tm2.2Vlf Véronique Lefebvre lab33 MGI: 3641205 Mouse: Ai9: B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J The Jackson Laboratory Stock No: 007909 Mouse: Dlx1/2CreERT2: Tg(I12b-cre/ERT2,-ALPP)37Fsh/J The Jackson Laboratory Stock No: 014600 Mouse: PvalbCre: B6;129P2-Pvalbtm1(cre)Arbr/J The Jackson Laboratory Stock No: 008069 Software and algorithms BackSPIN69 https://github.com/linnarsson-lab/BackSPIN N/A Clampfit Molecular Devices version 10.7 DESeq239 https://github.com/mikelove/DESeq2 version 1.34.0 Emmeans https://github.com/cran/emmeans version 1.7.0 ImageJ https://imagej.net/ij/index.html Version 1.53j (Continued on next page) e1 Neuron 111, 3590–3603.e1–e5, November 15, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER R https://www.r-project.org/ version 4.1.1 RStudio https://www.rstudio.com version 1.4.1717 Samtools70 http://www.htslib.org/ version 1.10 Seurat46 https://satijalab.org/seurat/index.html version 4.0.5 SPSS IBM version 17
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Millipore is a verified supplier 
