Review




Structured Review

Proteintech mab3406
Mab3406, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mab3406/DPP7+Antibody/pm32717133-31-57-59
Average 93 stars, based on 4 article reviews
mab3406 - by Bioz Stars, 2026-09
93/100 stars

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Related Articles

Membrane:

Article Title: Melflufen, a peptide‐conjugated alkylator, is an efficient anti‐neo‐plastic drug in breast cancer cell lines
Article Snippet: Total protein content was extracted from cells using RIPA buffer (50 mmol L ‐1 tris‐HCl (pH 7.4), 150 mmol L ‐1 NaCl, 0.5% Igepal, 5 mmol L ‐1 EDTA (pH 8.0), 0.1% SDS supplemented with Halt Protease, and Phosphatase Inhibitor cocktail (Thermo Fisher). .. Equal volume of each sample was resolved on Tris‐glycine SDS‐polyacrylamide gels and transferred to a nitrocellulose membrane (Santa Cruz Biotechnology), blocked in blocking buffer (5% skim milk powder in PBS), and probed with primary antibodys overnight at 4°C (Actin B, Li‐Cor Cat. No. 926‐42212; γH2AX, Cell Signaling Technology, Cat. No. 80 312; H2AX, R&D Systems, Cat. No. MAB3406; DPP7, Proteintech, Cat. No. 19018‐1‐AP; LAP3, Santa Cruz Biotechnology, Cat. No. sc‐398601; CD13, Santa Cruz Biotechnology, Cat. No. sc‐51522; cleaved PARP (Asp214), Cell Signaling Technology, Cat. No. 5625; cleaved Caspase‐3 (Asp175), Cell Signaling Technology, Cat. No. 9664). .. Proteins were detected using horseradish peroxidase‐conjugated secondary antibodies (Santa Cruz Biotechnology) and Pierce ECL detection substrate (Thermo Fisher).

Article Title: Melflufen, a peptide-conjugated alkylator, is an efficient anti-neo-plastic drug in breast cancer cell lines.
Article Snippet: Total protein content was extracted from cells using RIPA buffer (50 mmol L-1 tris-HCl (pH 7.4), 150 mmol L-1 NaCl, 0.5% Igepal, 5 mmol L-1 EDTA (pH 8.0), 0.1% SDS supplemented with Halt Protease, and Phosphatase Inhibitor cocktail (Thermo Fisher). .. Equal volume of each sample was resolved on Tris-glycine SDS-polyacrylamide gels and transferred to a nitrocellulose membrane (Santa Cruz Biotechnology), blocked in blocking buffer (5% skim milk powder in PBS), and probed with primary antibodys overnight at 4°C (Actin B, Li-Cor Cat. No. 926-42212; γH2AX, Cell Signaling Technology, Cat. No. 80 312; H2AX, R&D Systems, Cat. No. MAB3406; DPP7, Proteintech, Cat. No. 19018-1-AP; LAP3, Santa Cruz Biotechnology, Cat. No. sc-398601; CD13, Santa Cruz Biotechnology, Cat. No. sc-51522; cleaved PARP (Asp214), Cell Signaling Technology, Cat. No. 5625; cleaved Caspase-3 (Asp175), Cell Signaling Technology, Cat. No. 9664). .. Proteins were detected using horseradish peroxidase-conjugated secondary antibodies (Santa Cruz Biotechnology) and Pierce ECL detection substrate (Thermo Fisher).

Blocking Assay:

Article Title: Melflufen, a peptide‐conjugated alkylator, is an efficient anti‐neo‐plastic drug in breast cancer cell lines
Article Snippet: Total protein content was extracted from cells using RIPA buffer (50 mmol L ‐1 tris‐HCl (pH 7.4), 150 mmol L ‐1 NaCl, 0.5% Igepal, 5 mmol L ‐1 EDTA (pH 8.0), 0.1% SDS supplemented with Halt Protease, and Phosphatase Inhibitor cocktail (Thermo Fisher). .. Equal volume of each sample was resolved on Tris‐glycine SDS‐polyacrylamide gels and transferred to a nitrocellulose membrane (Santa Cruz Biotechnology), blocked in blocking buffer (5% skim milk powder in PBS), and probed with primary antibodys overnight at 4°C (Actin B, Li‐Cor Cat. No. 926‐42212; γH2AX, Cell Signaling Technology, Cat. No. 80 312; H2AX, R&D Systems, Cat. No. MAB3406; DPP7, Proteintech, Cat. No. 19018‐1‐AP; LAP3, Santa Cruz Biotechnology, Cat. No. sc‐398601; CD13, Santa Cruz Biotechnology, Cat. No. sc‐51522; cleaved PARP (Asp214), Cell Signaling Technology, Cat. No. 5625; cleaved Caspase‐3 (Asp175), Cell Signaling Technology, Cat. No. 9664). .. Proteins were detected using horseradish peroxidase‐conjugated secondary antibodies (Santa Cruz Biotechnology) and Pierce ECL detection substrate (Thermo Fisher).

Article Title: Melflufen, a peptide-conjugated alkylator, is an efficient anti-neo-plastic drug in breast cancer cell lines.
Article Snippet: Total protein content was extracted from cells using RIPA buffer (50 mmol L-1 tris-HCl (pH 7.4), 150 mmol L-1 NaCl, 0.5% Igepal, 5 mmol L-1 EDTA (pH 8.0), 0.1% SDS supplemented with Halt Protease, and Phosphatase Inhibitor cocktail (Thermo Fisher). .. Equal volume of each sample was resolved on Tris-glycine SDS-polyacrylamide gels and transferred to a nitrocellulose membrane (Santa Cruz Biotechnology), blocked in blocking buffer (5% skim milk powder in PBS), and probed with primary antibodys overnight at 4°C (Actin B, Li-Cor Cat. No. 926-42212; γH2AX, Cell Signaling Technology, Cat. No. 80 312; H2AX, R&D Systems, Cat. No. MAB3406; DPP7, Proteintech, Cat. No. 19018-1-AP; LAP3, Santa Cruz Biotechnology, Cat. No. sc-398601; CD13, Santa Cruz Biotechnology, Cat. No. sc-51522; cleaved PARP (Asp214), Cell Signaling Technology, Cat. No. 5625; cleaved Caspase-3 (Asp175), Cell Signaling Technology, Cat. No. 9664). .. Proteins were detected using horseradish peroxidase-conjugated secondary antibodies (Santa Cruz Biotechnology) and Pierce ECL detection substrate (Thermo Fisher).



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