antibodies targeting m6a  (Proteintech)

Journal: bioRxiv

Article Title: METTL14-dependent m 6 A modification restrains interferon signaling to prevent myocarditis and dilated Cardiomyopathy

doi: 10.64898/2026.04.02.716218

Figure Lengend Snippet: Loss of METTL14 alters m6A modification, leading to mitochondrial dysfunction, cGAS-STING-IFN-I activation, and necroptosis. These parallel pathways converge to drive cardiomyopathy and heart failure.

Article Snippet: Membranes were then blocked with 5% nonfat dry milk in 1× PBST for 1 h at room temperature and incubated overnight at 4 °C with an anti-m6A antibody (1:5,000; Cell Signaling Technology, Cat. #56593).

Techniques: Modification, Activation Assay

Journal: Cell Adhesion & Migration

Article Title: N6-methyladenosine modification of NEU1 mediated by METTL3 exacerbates angiotensin II-induced atrial fibrillation

doi: 10.1080/19336918.2026.2650272

Figure Lengend Snippet: NEU1 mRNA stability is regulated by m6A modification. (A) Total m6A levels in RNA from mouse atrial tissues and atrial fibroblasts were measured using an m6A quantification kit. (B) Western blot analysis of METTL3 expression in atrial tissues and atrial fibroblasts. (C) RNA immunoprecipitation (RIP) assay confirming the interaction between NEU1 and METTL3 in atrial fibroblasts. (D) Western blot analysis of METTL3 expression in primary mouse atrial fibroblasts. (E) MeRIP-qPCR assay detecting the m6A modification level on NEU1 mRNA in primary mouse atrial fibroblasts. (F) NEU1 mRNA stability assessed by qPCR in atrial fibroblasts treated with actinomycin D at 0, 3, and 6 hours post-transfection with sh-NC or sh-METTL3.

Article Snippet: For each sample, 50 μg of fragmented RNA was reserved as input, and the remaining RNA was incubated with 5 μg of anti-m6A antibody (68055–1-Ig, Proteintech) or IgG antibody (30000–0-AP, Proteintech) at 4°C overnight with gentle rotation, followed by incubation with Protein A/G magnetic beads (88802, Thermo Fisher Scientific) for 2 hours at 4°C.

Techniques: Modification, Western Blot, Expressing, RNA Immunoprecipitation, Transfection