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Nycomed density gradient by lymphoprep
Density Gradient By Lymphoprep, supplied by Nycomed, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lymphoprep/by+density+gradient+lymphoprep/pm42075662-75-32-36
Average 86 stars, based on 1 article reviews
density gradient by lymphoprep - by Bioz Stars, 2026-09
86/100 stars

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Isolation:

Article Title: Bacterial extracellular vesicles
Article Snippet: .. Briefly, PBMCs are isolated from heparinized venous blood from CMs by gradient centrifugation using Lymphoprep (Nycomed, Oslo, Norway) or from mouse spleens or bone marrow using the magnetic bead-based Human Blood Dendritic cell isolation kit (Miltenyi Biotech, Cambridge, MA). ..

Article Title: Novel pathogenic variants in CTLA4 and LRBA immune dysregulation: Reduced CTLA-4 expression with normal expression of co-stimulatory surface molecules.
Article Snippet: Background: Genetic variants in Cytotoxic T-Lymphocyte-associated protein 4 (CTLA4) and LPS responsive beige-like anchor protein (LRBA), involved in the same biological pathways, are implicated as monogenic causes of Common Variable Immunodeficiency Disorder (CVID).. The pitfall in the recognition of CVID possibly related to CTLA-4 haploinsufficiency or LRBA deficiency is a clinical picture that is very heterogeneous.. In the present study, we illustrate this challenge by means of clinical and immunological analysis of five patients with novel genetic variants in CTLA4 and LRBA.

Article Title: Recommended or high daily intakes of plant stanol esters do not affect ex vivo T-cell derived cytokine production in immunologically healthy volunteers
Article Snippet: .. From the subjects in study 1, PBMCs were freshly isolated from heparinized blood using Lymphoprep (Nycomed Pharma, Asker, Norway) gradient centrifugation according to the manufacturer’s instructions. .. Cells were seeded (10 6 /ml) in a 24-wells flat-bottom culture plate (Corning Incorporated, Corning, New York, USA) and cultured in RPMI 1640 medium (Gibco, Thermo Fisher Scientific, Waltham, Massachusetts, USA) supplemented with 1 % sodium pyruvate, 1 % penicillin/streptomycin and 1 % human serum pool, heat inactivated at 56°C for 30 min. 5 μg/ml Phytohemagglutinin-M (Roche Diagnostic Systems, Mannheim, Germany) was used to induce T-cell proliferation.

Article Title: Understanding the mechanisms underlying the lack of response to Janus kinase inhibition in ulcerative colitis
Article Snippet: .. Human peripheral blood mononuclear cells (PBMCs) were isolated from buffy coats of healthy volunteers over a Lymphoprep (Nycomed Pharma) gradient according to standard procedures. .. Monocytes were purified from PBMCs by magnetic cell sorting using CD14 Microbeads (Miltenyi Biotec).

Article Title: Differential effects of tofacitinib on macrophage activation contribute to lack of response in ulcerative colitis patients
Article Snippet: .. Human peripheral blood mononuclear cells (PBMCs) were isolated from buffy coats (Banc de Sang i Teixits, Barcelona, Spain) of healthy volunteers ( n = 15) or UC patients ( n = 8) over a Lymphoprep (Nycomed Pharma) gradient according to standard procedures. .. Monocytes were purified from PBMCs by magnetic cell sorting using CD14 Microbeads (Miltenyi Biotec).

Gradient Centrifugation:

Article Title: Bacterial extracellular vesicles
Article Snippet: .. Briefly, PBMCs are isolated from heparinized venous blood from CMs by gradient centrifugation using Lymphoprep (Nycomed, Oslo, Norway) or from mouse spleens or bone marrow using the magnetic bead-based Human Blood Dendritic cell isolation kit (Miltenyi Biotech, Cambridge, MA). ..

Article Title: Novel pathogenic variants in CTLA4 and LRBA immune dysregulation: Reduced CTLA-4 expression with normal expression of co-stimulatory surface molecules.
Article Snippet: Background: Genetic variants in Cytotoxic T-Lymphocyte-associated protein 4 (CTLA4) and LPS responsive beige-like anchor protein (LRBA), involved in the same biological pathways, are implicated as monogenic causes of Common Variable Immunodeficiency Disorder (CVID).. The pitfall in the recognition of CVID possibly related to CTLA-4 haploinsufficiency or LRBA deficiency is a clinical picture that is very heterogeneous.. In the present study, we illustrate this challenge by means of clinical and immunological analysis of five patients with novel genetic variants in CTLA4 and LRBA.

Article Title: Selective inhibition of HDAC class IIA as therapeutic intervention for KMT2A- rearranged acute lymphoblastic leukemia
Article Snippet: Leukemic cells were transduced via spinfection by incubation with a single dose of lentiviral supernatant supplemented with 4 μg/ml Polybrene (Bio-Connect) for shRNA-mediated knock-down experiments. .. Puromycin selection started 24 hours post-transduction with 1–1.5 μg/ml (Sigma Aldrich) for 72 hours, whereafter density gradient centrifugation with Lymphoprep (Nycomed Pharma) was used to remove dead cells as previously described . .. SEM transduced with GFP-luc construct was sorted based on eGFP signal using the SH800S Cell sorter (Sony Biotechnology).

Article Title: Recommended or high daily intakes of plant stanol esters do not affect ex vivo T-cell derived cytokine production in immunologically healthy volunteers
Article Snippet: .. From the subjects in study 1, PBMCs were freshly isolated from heparinized blood using Lymphoprep (Nycomed Pharma, Asker, Norway) gradient centrifugation according to the manufacturer’s instructions. .. Cells were seeded (10 6 /ml) in a 24-wells flat-bottom culture plate (Corning Incorporated, Corning, New York, USA) and cultured in RPMI 1640 medium (Gibco, Thermo Fisher Scientific, Waltham, Massachusetts, USA) supplemented with 1 % sodium pyruvate, 1 % penicillin/streptomycin and 1 % human serum pool, heat inactivated at 56°C for 30 min. 5 μg/ml Phytohemagglutinin-M (Roche Diagnostic Systems, Mannheim, Germany) was used to induce T-cell proliferation.

Article Title: Optimizing biofilm inhibitors: Balancing activity and toxicity in 2N-aminated 5-aryl-2-aminoimidazoles.
Article Snippet: To evaluate the effect of amination on biofilm inhibition against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus, representative compounds of two previously described 5-aryl-2-aminoimidazole (5-Ar-2AI) classes were aminated by installing an amino group at the end of the substituted n-alkyl chain.. Amination led to an improvement in activity for one of the two classes, the 2N-substituted 5-Ar-2-AI class.. Based on these findings, a more extensive library of 2N-substituted-aminated 5-Ar-2-AIs was synthesized having different n-alkyl and halogen substitutions on the 2N-position and the 4(5)-phenyl ring, respectively.

Article Title: Activated Charcoal-Alginate Platform for Simultaneous Local Delivery of Bioactive Agents: At the Nexus of Antimicrobial and Cytotoxic Activity of Zn 2+ Ions
Article Snippet: .. Peripheral blood mononuclear cells (PBMC) were separated from whole heparinized blood samples (obtained from healthy volunteers) by Lymphoprep (Nycomed, Oslo, Norway) gradient centrifugation. .. Interface cells, washed three times with Haemaccel (aqueous solution supplemented with 145 mM Na + , 5.1 mM K + , 6.2 mM Ca 2+ , 145 Mm Cl − and 35 g/L gelatin polymers, pH 7.4, Abbott, Chicago, IL, USA), were counted and re-suspended in complete nutrient medium.

Cell Isolation:

Article Title: Bacterial extracellular vesicles
Article Snippet: .. Briefly, PBMCs are isolated from heparinized venous blood from CMs by gradient centrifugation using Lymphoprep (Nycomed, Oslo, Norway) or from mouse spleens or bone marrow using the magnetic bead-based Human Blood Dendritic cell isolation kit (Miltenyi Biotech, Cambridge, MA). ..

Selection:

Article Title: Selective inhibition of HDAC class IIA as therapeutic intervention for KMT2A- rearranged acute lymphoblastic leukemia
Article Snippet: Leukemic cells were transduced via spinfection by incubation with a single dose of lentiviral supernatant supplemented with 4 μg/ml Polybrene (Bio-Connect) for shRNA-mediated knock-down experiments. .. Puromycin selection started 24 hours post-transduction with 1–1.5 μg/ml (Sigma Aldrich) for 72 hours, whereafter density gradient centrifugation with Lymphoprep (Nycomed Pharma) was used to remove dead cells as previously described . .. SEM transduced with GFP-luc construct was sorted based on eGFP signal using the SH800S Cell sorter (Sony Biotechnology).

Cell Culture:

Article Title: Optimizing biofilm inhibitors: Balancing activity and toxicity in 2N-aminated 5-aryl-2-aminoimidazoles.
Article Snippet: To evaluate the effect of amination on biofilm inhibition against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus, representative compounds of two previously described 5-aryl-2-aminoimidazole (5-Ar-2AI) classes were aminated by installing an amino group at the end of the substituted n-alkyl chain.. Amination led to an improvement in activity for one of the two classes, the 2N-substituted 5-Ar-2-AI class.. Based on these findings, a more extensive library of 2N-substituted-aminated 5-Ar-2-AIs was synthesized having different n-alkyl and halogen substitutions on the 2N-position and the 4(5)-phenyl ring, respectively.



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