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pgl3 luciferase construct  (Addgene inc)


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    Structured Review

    Addgene inc pgl3 luciferase construct
    Pgl3 Luciferase Construct, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/luciferase+construct/pGL3+(Plasmid+%2348743)/pm41803560-301-1-5
    Average 94 stars, based on 59 article reviews
    pgl3 luciferase construct - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Transfection:

    Article Title: Environmental Xenoestrogens Super-Activate a Variant Murine ER Beta in Cholangiocytes
    Article Snippet: .. 603B and LTPA cells were transiently transfected in 24-well plates with 0.25 μg total DNA per well (pcDNA3.1 expression vector encoding the cDNAs for either the mERα, mERβv1, mERβv2, mouse c/EBPβ or an empty construct (ie, pcDNA3.1); an oestrogen-responsive reporter gene luciferase construct, either (ERE) 3 -pGL3promoter ( Axon et al. , 2012 ) or 3XERE TATA Luc ( ; obtained via Adgene plasmid no. 11354) and a control plasmid (RL-TK) encoding the Renilla luciferase protein under the regulation of a constitutive thymidine kinase promoter to control for transfection efficiency between wells at a ratio of 6, 6, 1) using Effectene reagent (Qiagen, Manchester, UK), according to the manufacturer’s instructions. ..

    Article Title: LSR overexpression induces chemoresistance in triple negative breast cancer cells through MDR1 upregulation and apoptosis attenuation.
    Article Snippet: .. After 24 hours of incubation, cells were cotransfected with plasmids encoding the MDR1 promoter-firefly-luciferase (pMDR1–1202, Cat: 37627, Addgene) and the SV40 promoter-Renilla luciferase with luciferase construct (pRL-SV40, Cat: 27163, Addgene) using EndoFectin Transfection Reagents (Cat: EF013, GeneCopoeia). .. After 48 hours, luciferase activities were measured using the Dual-Luciferase Assay System (Cat: E1910, Promega) according to the manufacturer’s instructions.

    Article Title: LSR overexpression induces chemoresistance in triple negative breast cancer cells through MDR1 upregulation and apoptosis attenuation
    Article Snippet: .. After 24 hours of incubation, cells were co-transfected with plasmids encoding the MDR1 promoter-firefly-luciferase (pMDR1–1202, Cat: 37627, Addgene) and the SV40 promoter-Renilla luciferase with luciferase construct (pRL-SV40, Cat: 27163, Addgene) using EndoFectin Transfection Reagents (Cat: EF013, GeneCopoeia). .. After 48 hours, luciferase activities were measured using the Dual-Luciferase Assay System (Cat: E1910, Promega) according to the manufacturer’s instructions.

    Expressing:

    Article Title: Environmental Xenoestrogens Super-Activate a Variant Murine ER Beta in Cholangiocytes
    Article Snippet: .. 603B and LTPA cells were transiently transfected in 24-well plates with 0.25 μg total DNA per well (pcDNA3.1 expression vector encoding the cDNAs for either the mERα, mERβv1, mERβv2, mouse c/EBPβ or an empty construct (ie, pcDNA3.1); an oestrogen-responsive reporter gene luciferase construct, either (ERE) 3 -pGL3promoter ( Axon et al. , 2012 ) or 3XERE TATA Luc ( ; obtained via Adgene plasmid no. 11354) and a control plasmid (RL-TK) encoding the Renilla luciferase protein under the regulation of a constitutive thymidine kinase promoter to control for transfection efficiency between wells at a ratio of 6, 6, 1) using Effectene reagent (Qiagen, Manchester, UK), according to the manufacturer’s instructions. ..

    Article Title: Stratification of radiosensitive brain metastases based on an actionable S100A9/RAGE resistance mechanism.
    Article Snippet: .. Briefly, a cell suspension containing 105 E0771-P cells expressing a Luciferase construct (blasticidin resistance; 19166, Addgene)79 in a volume of 100 μl was injected in the left cardiac ventricle of anesthetized 4- to 6-week-old C57BL/6 mice. ..

    Article Title: Stratification of radiosensitive brain metastases based on an actionable S100A9/RAGE resistance mechanism
    Article Snippet: .. Briefly, a cell suspension containing 10 5 E0771-P cells expressing a Luciferase construct (blasticidin resistance; 19166, Addgene) in a volume of 100 μl was injected in the left cardiac ventricle of anesthetized 4- to 6-week-old C57BL/6 mice. ..

    Article Title: STAT3 labels a subpopulation of reactive astrocytes required for brain metastasis.
    Article Snippet: .. Briefly, a cell suspension containing 105 B16/F10-P cells expressing a luciferase construct (blasticidin resistance; 19166, Addgene)63, in a volume of 100 μ l, was injected in the left cardiac ventricle of anesthetized 4–6-week-old C57BL/6 mice. ..

    Construct:

    Article Title: Environmental Xenoestrogens Super-Activate a Variant Murine ER Beta in Cholangiocytes
    Article Snippet: .. 603B and LTPA cells were transiently transfected in 24-well plates with 0.25 μg total DNA per well (pcDNA3.1 expression vector encoding the cDNAs for either the mERα, mERβv1, mERβv2, mouse c/EBPβ or an empty construct (ie, pcDNA3.1); an oestrogen-responsive reporter gene luciferase construct, either (ERE) 3 -pGL3promoter ( Axon et al. , 2012 ) or 3XERE TATA Luc ( ; obtained via Adgene plasmid no. 11354) and a control plasmid (RL-TK) encoding the Renilla luciferase protein under the regulation of a constitutive thymidine kinase promoter to control for transfection efficiency between wells at a ratio of 6, 6, 1) using Effectene reagent (Qiagen, Manchester, UK), according to the manufacturer’s instructions. ..

    Article Title: Stratification of radiosensitive brain metastases based on an actionable S100A9/RAGE resistance mechanism.
    Article Snippet: .. Briefly, a cell suspension containing 105 E0771-P cells expressing a Luciferase construct (blasticidin resistance; 19166, Addgene)79 in a volume of 100 μl was injected in the left cardiac ventricle of anesthetized 4- to 6-week-old C57BL/6 mice. ..

    Article Title: LSR overexpression induces chemoresistance in triple negative breast cancer cells through MDR1 upregulation and apoptosis attenuation.
    Article Snippet: .. After 24 hours of incubation, cells were cotransfected with plasmids encoding the MDR1 promoter-firefly-luciferase (pMDR1–1202, Cat: 37627, Addgene) and the SV40 promoter-Renilla luciferase with luciferase construct (pRL-SV40, Cat: 27163, Addgene) using EndoFectin Transfection Reagents (Cat: EF013, GeneCopoeia). .. After 48 hours, luciferase activities were measured using the Dual-Luciferase Assay System (Cat: E1910, Promega) according to the manufacturer’s instructions.

    Article Title: Stratification of radiosensitive brain metastases based on an actionable S100A9/RAGE resistance mechanism
    Article Snippet: .. Briefly, a cell suspension containing 10 5 E0771-P cells expressing a Luciferase construct (blasticidin resistance; 19166, Addgene) in a volume of 100 μl was injected in the left cardiac ventricle of anesthetized 4- to 6-week-old C57BL/6 mice. ..

    Article Title: LSR overexpression induces chemoresistance in triple negative breast cancer cells through MDR1 upregulation and apoptosis attenuation
    Article Snippet: .. After 24 hours of incubation, cells were co-transfected with plasmids encoding the MDR1 promoter-firefly-luciferase (pMDR1–1202, Cat: 37627, Addgene) and the SV40 promoter-Renilla luciferase with luciferase construct (pRL-SV40, Cat: 27163, Addgene) using EndoFectin Transfection Reagents (Cat: EF013, GeneCopoeia). .. After 48 hours, luciferase activities were measured using the Dual-Luciferase Assay System (Cat: E1910, Promega) according to the manufacturer’s instructions.

    Article Title: STAT3 labels a subpopulation of reactive astrocytes required for brain metastasis.
    Article Snippet: .. Briefly, a cell suspension containing 105 B16/F10-P cells expressing a luciferase construct (blasticidin resistance; 19166, Addgene)63, in a volume of 100 μ l, was injected in the left cardiac ventricle of anesthetized 4–6-week-old C57BL/6 mice. ..

    Luciferase:

    Article Title: Environmental Xenoestrogens Super-Activate a Variant Murine ER Beta in Cholangiocytes
    Article Snippet: .. 603B and LTPA cells were transiently transfected in 24-well plates with 0.25 μg total DNA per well (pcDNA3.1 expression vector encoding the cDNAs for either the mERα, mERβv1, mERβv2, mouse c/EBPβ or an empty construct (ie, pcDNA3.1); an oestrogen-responsive reporter gene luciferase construct, either (ERE) 3 -pGL3promoter ( Axon et al. , 2012 ) or 3XERE TATA Luc ( ; obtained via Adgene plasmid no. 11354) and a control plasmid (RL-TK) encoding the Renilla luciferase protein under the regulation of a constitutive thymidine kinase promoter to control for transfection efficiency between wells at a ratio of 6, 6, 1) using Effectene reagent (Qiagen, Manchester, UK), according to the manufacturer’s instructions. ..

    Article Title: Stratification of radiosensitive brain metastases based on an actionable S100A9/RAGE resistance mechanism.
    Article Snippet: .. Briefly, a cell suspension containing 105 E0771-P cells expressing a Luciferase construct (blasticidin resistance; 19166, Addgene)79 in a volume of 100 μl was injected in the left cardiac ventricle of anesthetized 4- to 6-week-old C57BL/6 mice. ..

    Article Title: LSR overexpression induces chemoresistance in triple negative breast cancer cells through MDR1 upregulation and apoptosis attenuation.
    Article Snippet: .. After 24 hours of incubation, cells were cotransfected with plasmids encoding the MDR1 promoter-firefly-luciferase (pMDR1–1202, Cat: 37627, Addgene) and the SV40 promoter-Renilla luciferase with luciferase construct (pRL-SV40, Cat: 27163, Addgene) using EndoFectin Transfection Reagents (Cat: EF013, GeneCopoeia). .. After 48 hours, luciferase activities were measured using the Dual-Luciferase Assay System (Cat: E1910, Promega) according to the manufacturer’s instructions.

    Article Title: Stratification of radiosensitive brain metastases based on an actionable S100A9/RAGE resistance mechanism
    Article Snippet: .. Briefly, a cell suspension containing 10 5 E0771-P cells expressing a Luciferase construct (blasticidin resistance; 19166, Addgene) in a volume of 100 μl was injected in the left cardiac ventricle of anesthetized 4- to 6-week-old C57BL/6 mice. ..

    Article Title: LSR overexpression induces chemoresistance in triple negative breast cancer cells through MDR1 upregulation and apoptosis attenuation
    Article Snippet: .. After 24 hours of incubation, cells were co-transfected with plasmids encoding the MDR1 promoter-firefly-luciferase (pMDR1–1202, Cat: 37627, Addgene) and the SV40 promoter-Renilla luciferase with luciferase construct (pRL-SV40, Cat: 27163, Addgene) using EndoFectin Transfection Reagents (Cat: EF013, GeneCopoeia). .. After 48 hours, luciferase activities were measured using the Dual-Luciferase Assay System (Cat: E1910, Promega) according to the manufacturer’s instructions.

    Article Title: STAT3 labels a subpopulation of reactive astrocytes required for brain metastasis.
    Article Snippet: .. Briefly, a cell suspension containing 105 B16/F10-P cells expressing a luciferase construct (blasticidin resistance; 19166, Addgene)63, in a volume of 100 μ l, was injected in the left cardiac ventricle of anesthetized 4–6-week-old C57BL/6 mice. ..

    Plasmid Preparation:

    Article Title: Environmental Xenoestrogens Super-Activate a Variant Murine ER Beta in Cholangiocytes
    Article Snippet: .. 603B and LTPA cells were transiently transfected in 24-well plates with 0.25 μg total DNA per well (pcDNA3.1 expression vector encoding the cDNAs for either the mERα, mERβv1, mERβv2, mouse c/EBPβ or an empty construct (ie, pcDNA3.1); an oestrogen-responsive reporter gene luciferase construct, either (ERE) 3 -pGL3promoter ( Axon et al. , 2012 ) or 3XERE TATA Luc ( ; obtained via Adgene plasmid no. 11354) and a control plasmid (RL-TK) encoding the Renilla luciferase protein under the regulation of a constitutive thymidine kinase promoter to control for transfection efficiency between wells at a ratio of 6, 6, 1) using Effectene reagent (Qiagen, Manchester, UK), according to the manufacturer’s instructions. ..

    Control:

    Article Title: Environmental Xenoestrogens Super-Activate a Variant Murine ER Beta in Cholangiocytes
    Article Snippet: .. 603B and LTPA cells were transiently transfected in 24-well plates with 0.25 μg total DNA per well (pcDNA3.1 expression vector encoding the cDNAs for either the mERα, mERβv1, mERβv2, mouse c/EBPβ or an empty construct (ie, pcDNA3.1); an oestrogen-responsive reporter gene luciferase construct, either (ERE) 3 -pGL3promoter ( Axon et al. , 2012 ) or 3XERE TATA Luc ( ; obtained via Adgene plasmid no. 11354) and a control plasmid (RL-TK) encoding the Renilla luciferase protein under the regulation of a constitutive thymidine kinase promoter to control for transfection efficiency between wells at a ratio of 6, 6, 1) using Effectene reagent (Qiagen, Manchester, UK), according to the manufacturer’s instructions. ..

    Suspension:

    Article Title: Stratification of radiosensitive brain metastases based on an actionable S100A9/RAGE resistance mechanism.
    Article Snippet: .. Briefly, a cell suspension containing 105 E0771-P cells expressing a Luciferase construct (blasticidin resistance; 19166, Addgene)79 in a volume of 100 μl was injected in the left cardiac ventricle of anesthetized 4- to 6-week-old C57BL/6 mice. ..

    Article Title: Stratification of radiosensitive brain metastases based on an actionable S100A9/RAGE resistance mechanism
    Article Snippet: .. Briefly, a cell suspension containing 10 5 E0771-P cells expressing a Luciferase construct (blasticidin resistance; 19166, Addgene) in a volume of 100 μl was injected in the left cardiac ventricle of anesthetized 4- to 6-week-old C57BL/6 mice. ..

    Article Title: STAT3 labels a subpopulation of reactive astrocytes required for brain metastasis.
    Article Snippet: .. Briefly, a cell suspension containing 105 B16/F10-P cells expressing a luciferase construct (blasticidin resistance; 19166, Addgene)63, in a volume of 100 μ l, was injected in the left cardiac ventricle of anesthetized 4–6-week-old C57BL/6 mice. ..

    Injection:

    Article Title: Stratification of radiosensitive brain metastases based on an actionable S100A9/RAGE resistance mechanism.
    Article Snippet: .. Briefly, a cell suspension containing 105 E0771-P cells expressing a Luciferase construct (blasticidin resistance; 19166, Addgene)79 in a volume of 100 μl was injected in the left cardiac ventricle of anesthetized 4- to 6-week-old C57BL/6 mice. ..

    Article Title: Stratification of radiosensitive brain metastases based on an actionable S100A9/RAGE resistance mechanism
    Article Snippet: .. Briefly, a cell suspension containing 10 5 E0771-P cells expressing a Luciferase construct (blasticidin resistance; 19166, Addgene) in a volume of 100 μl was injected in the left cardiac ventricle of anesthetized 4- to 6-week-old C57BL/6 mice. ..

    Article Title: STAT3 labels a subpopulation of reactive astrocytes required for brain metastasis.
    Article Snippet: .. Briefly, a cell suspension containing 105 B16/F10-P cells expressing a luciferase construct (blasticidin resistance; 19166, Addgene)63, in a volume of 100 μ l, was injected in the left cardiac ventricle of anesthetized 4–6-week-old C57BL/6 mice. ..

    Incubation:

    Article Title: LSR overexpression induces chemoresistance in triple negative breast cancer cells through MDR1 upregulation and apoptosis attenuation.
    Article Snippet: .. After 24 hours of incubation, cells were cotransfected with plasmids encoding the MDR1 promoter-firefly-luciferase (pMDR1–1202, Cat: 37627, Addgene) and the SV40 promoter-Renilla luciferase with luciferase construct (pRL-SV40, Cat: 27163, Addgene) using EndoFectin Transfection Reagents (Cat: EF013, GeneCopoeia). .. After 48 hours, luciferase activities were measured using the Dual-Luciferase Assay System (Cat: E1910, Promega) according to the manufacturer’s instructions.

    Article Title: LSR overexpression induces chemoresistance in triple negative breast cancer cells through MDR1 upregulation and apoptosis attenuation
    Article Snippet: .. After 24 hours of incubation, cells were co-transfected with plasmids encoding the MDR1 promoter-firefly-luciferase (pMDR1–1202, Cat: 37627, Addgene) and the SV40 promoter-Renilla luciferase with luciferase construct (pRL-SV40, Cat: 27163, Addgene) using EndoFectin Transfection Reagents (Cat: EF013, GeneCopoeia). .. After 48 hours, luciferase activities were measured using the Dual-Luciferase Assay System (Cat: E1910, Promega) according to the manufacturer’s instructions.



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    Image Search Results


    a. Schematic depicting in vitro validation of niche labeling efficiency in VO-PyMT cells transduced with a GFP/Luciferase construct and the mCherry niche labeling system (VO-sLP) , . Labeling VO-sLP cells were co-cultured with recipient, non-transduced, HEK293T cells. b. Flow cytometry results showing labeling capacity of VO-sLP cells. Plots show GFP and mCherry signals in either monocultures of HEK293T cells (left panel) or co-cultures of VO-sLP cells and HEK293T cells. Cells depicted resulted from excluding cell debris, doublets and non-viable cells (SytoxBlue-positive) by gating using FlowJo. Data is representative of 3 independent experiments. Values inside the plots indicate relative percentages of cells within each gate. c. Representative flow cytometry plots showing gating strategy to sort metastatic cellular fractions from brain, lung, liver and bone single-cell suspensions. Data is representative of 3 independent experiments. Numbers within plots indicate relative percentages of each cell population defined by the corresponding gates. d. Average expression levels of canonical markers of identified cell types in scRNA-seq data from murine brain, lung, liver, and bone tissues colonized by VO-sLP cells. Dot sizes depict the percentage of cells in each cluster expressing the corresponding gene. Color scale indicates average expression levels. Clusters (cell types) are indicated in the y-axis, while genes are indicated in the x-axis.

    Journal: bioRxiv

    Article Title: Single-cell profiling of synchronous multi-organ metastasis reveals a systemic CD74 + lipid-associated macrophage niche driving polymetastatic breast cancer

    doi: 10.64898/2026.01.31.701004

    Figure Lengend Snippet: a. Schematic depicting in vitro validation of niche labeling efficiency in VO-PyMT cells transduced with a GFP/Luciferase construct and the mCherry niche labeling system (VO-sLP) , . Labeling VO-sLP cells were co-cultured with recipient, non-transduced, HEK293T cells. b. Flow cytometry results showing labeling capacity of VO-sLP cells. Plots show GFP and mCherry signals in either monocultures of HEK293T cells (left panel) or co-cultures of VO-sLP cells and HEK293T cells. Cells depicted resulted from excluding cell debris, doublets and non-viable cells (SytoxBlue-positive) by gating using FlowJo. Data is representative of 3 independent experiments. Values inside the plots indicate relative percentages of cells within each gate. c. Representative flow cytometry plots showing gating strategy to sort metastatic cellular fractions from brain, lung, liver and bone single-cell suspensions. Data is representative of 3 independent experiments. Numbers within plots indicate relative percentages of each cell population defined by the corresponding gates. d. Average expression levels of canonical markers of identified cell types in scRNA-seq data from murine brain, lung, liver, and bone tissues colonized by VO-sLP cells. Dot sizes depict the percentage of cells in each cluster expressing the corresponding gene. Color scale indicates average expression levels. Clusters (cell types) are indicated in the y-axis, while genes are indicated in the x-axis.

    Article Snippet: To identify and isolate cancer cells from animal tissues, and quantify metastatic burden, VO-PyMT cells and Py8119 cells were genetically engineered by lentiviral transduction to express a green fluorescent protein (GFP) and luciferase gene construct (pCDH-EF1a-eFFly-eGFP, Addgene Plasmid #104834).

    Techniques: In Vitro, Biomarker Discovery, Labeling, Transduction, Luciferase, Construct, Cell Culture, Flow Cytometry, Single Cell, Expressing

    Qu-SeNPs activate WNT and BMP signaling pathways in osteoblasts . ( A ) Transiently transfected MC3T3-E1 cells with Axin-2 and (B) BRE reporter construct were treated free Qu (1 µg mL -1 ) or Qu-SeNPs at equivalent Qu concentration (75 ng mL -1 ) (n = 4; *p<0.05, **p<0.01, ***p<0.001 vs. control; one-way ANOVA with Dunnett’s multiple comparisons test, and # p<0.05, ## p<0.01; unpaired t-test). Luciferase activities were assessed in cell lysates after 48 h and normalized to Renilla luciferase activity, as detailed in the Materials and Methods section. MC3T3-E1 cells were treated with free Qu (1 µg mL -1 ) or Qu-SeNPs at an equivalent Qu concentration (75 ng mL -1 ) for the time indicated in the figure. Protein lysates were subsequently collected. Western blotting revealed the stabilization of (C) β-catenin and the (D) phosphorylation of Smad 1/5/8 molecules. β-actin was a loading control and normalized for densitometric analysis of Western blot bands. The relative expression level is compared to the control (n = 3; *p<0.05, **p<0.01, ***p<0.001 vs. 0 h; one-way ANOVA with Dunnett’s multiple comparisons test, and # p<0.05 vs. free Qu at similar time points; unpaired t-test.

    Journal: Aging and Disease

    Article Title: Bone Regeneration Enhanced by Quercetin-Capped Selenium Nanoparticles via miR206/Connexin43, WNT, and BMP signaling pathways

    doi: 10.14336/AD.2025.0025

    Figure Lengend Snippet: Qu-SeNPs activate WNT and BMP signaling pathways in osteoblasts . ( A ) Transiently transfected MC3T3-E1 cells with Axin-2 and (B) BRE reporter construct were treated free Qu (1 µg mL -1 ) or Qu-SeNPs at equivalent Qu concentration (75 ng mL -1 ) (n = 4; *p<0.05, **p<0.01, ***p<0.001 vs. control; one-way ANOVA with Dunnett’s multiple comparisons test, and # p<0.05, ## p<0.01; unpaired t-test). Luciferase activities were assessed in cell lysates after 48 h and normalized to Renilla luciferase activity, as detailed in the Materials and Methods section. MC3T3-E1 cells were treated with free Qu (1 µg mL -1 ) or Qu-SeNPs at an equivalent Qu concentration (75 ng mL -1 ) for the time indicated in the figure. Protein lysates were subsequently collected. Western blotting revealed the stabilization of (C) β-catenin and the (D) phosphorylation of Smad 1/5/8 molecules. β-actin was a loading control and normalized for densitometric analysis of Western blot bands. The relative expression level is compared to the control (n = 3; *p<0.05, **p<0.01, ***p<0.001 vs. 0 h; one-way ANOVA with Dunnett’s multiple comparisons test, and # p<0.05 vs. free Qu at similar time points; unpaired t-test.

    Article Snippet: Trizol reagent, SuperScript ІІ Reverse Transcriptase, and Renilla luciferase thymidine kinase construct was purchased from Invitrogen, USA.

    Techniques: Protein-Protein interactions, Transfection, Construct, Concentration Assay, Control, Luciferase, Activity Assay, Western Blot, Phospho-proteomics, Expressing