Journal: bioRxiv

Article Title: Single-cell profiling of synchronous multi-organ metastasis reveals a systemic CD74 + lipid-associated macrophage niche driving polymetastatic breast cancer

doi: 10.64898/2026.01.31.701004

Figure Lengend Snippet: a. Schematic depicting in vitro validation of niche labeling efficiency in VO-PyMT cells transduced with a GFP/Luciferase construct and the mCherry niche labeling system (VO-sLP) , . Labeling VO-sLP cells were co-cultured with recipient, non-transduced, HEK293T cells. b. Flow cytometry results showing labeling capacity of VO-sLP cells. Plots show GFP and mCherry signals in either monocultures of HEK293T cells (left panel) or co-cultures of VO-sLP cells and HEK293T cells. Cells depicted resulted from excluding cell debris, doublets and non-viable cells (SytoxBlue-positive) by gating using FlowJo. Data is representative of 3 independent experiments. Values inside the plots indicate relative percentages of cells within each gate. c. Representative flow cytometry plots showing gating strategy to sort metastatic cellular fractions from brain, lung, liver and bone single-cell suspensions. Data is representative of 3 independent experiments. Numbers within plots indicate relative percentages of each cell population defined by the corresponding gates. d. Average expression levels of canonical markers of identified cell types in scRNA-seq data from murine brain, lung, liver, and bone tissues colonized by VO-sLP cells. Dot sizes depict the percentage of cells in each cluster expressing the corresponding gene. Color scale indicates average expression levels. Clusters (cell types) are indicated in the y-axis, while genes are indicated in the x-axis.

Article Snippet: To identify and isolate cancer cells from animal tissues, and quantify metastatic burden, VO-PyMT cells and Py8119 cells were genetically engineered by lentiviral transduction to express a green fluorescent protein (GFP) and luciferase gene construct (pCDH-EF1a-eFFly-eGFP, Addgene Plasmid #104834).

Techniques: In Vitro, Biomarker Discovery, Labeling, Transduction, Luciferase, Construct, Cell Culture, Flow Cytometry, Single Cell, Expressing

Journal: Aging and Disease

Article Title: Bone Regeneration Enhanced by Quercetin-Capped Selenium Nanoparticles via miR206/Connexin43, WNT, and BMP signaling pathways

doi: 10.14336/AD.2025.0025

Figure Lengend Snippet: Qu-SeNPs activate WNT and BMP signaling pathways in osteoblasts . ( A ) Transiently transfected MC3T3-E1 cells with Axin-2 and (B) BRE reporter construct were treated free Qu (1 µg mL -1 ) or Qu-SeNPs at equivalent Qu concentration (75 ng mL -1 ) (n = 4; *p<0.05, **p<0.01, ***p<0.001 vs. control; one-way ANOVA with Dunnett’s multiple comparisons test, and # p<0.05, ## p<0.01; unpaired t-test). Luciferase activities were assessed in cell lysates after 48 h and normalized to Renilla luciferase activity, as detailed in the Materials and Methods section. MC3T3-E1 cells were treated with free Qu (1 µg mL -1 ) or Qu-SeNPs at an equivalent Qu concentration (75 ng mL -1 ) for the time indicated in the figure. Protein lysates were subsequently collected. Western blotting revealed the stabilization of (C) β-catenin and the (D) phosphorylation of Smad 1/5/8 molecules. β-actin was a loading control and normalized for densitometric analysis of Western blot bands. The relative expression level is compared to the control (n = 3; *p<0.05, **p<0.01, ***p<0.001 vs. 0 h; one-way ANOVA with Dunnett’s multiple comparisons test, and # p<0.05 vs. free Qu at similar time points; unpaired t-test.

Article Snippet: Trizol reagent, SuperScript ІІ Reverse Transcriptase, and Renilla luciferase thymidine kinase construct was purchased from Invitrogen, USA.

Techniques: Protein-Protein interactions, Transfection, Construct, Concentration Assay, Control, Luciferase, Activity Assay, Western Blot, Phospho-proteomics, Expressing