lpcat1  (Proteintech)

Journal: Cell Death Discovery

Article Title: Lysophosphatidylcholine acyltransferase 1 promotes head and neck squamous cell carcinoma progression by enhancing COX17-dependent oxidative phosphorylation

doi: 10.1038/s41420-026-02994-3

Figure Lengend Snippet: A Transcriptomic analysis of the TCGA database shows the mRNA expression levels of phosphatidylcholine-metabolizing enzymes, including LPCAT1, CEPT1, CHKA, PEMT, PCYT1A, LPCAT2, LPCAT3, and LPCAT4, in HNSCC ( n = 519) and normal tissues ( n = 44). B LPCAT1 protein expression in HNSCC ( n = 109) and normal tissues ( n = 70) from the Human Protein Atlas database. C Representative immunohistochemical staining of LPCAT1 protein in normal oral mucosa and HNSCC from the Human Protein Atlas database. Scale bar, 100 μm in the upper image, 20 μm in the lower image. LPCAT1 mRNA ( D ) and protein ( E ) in normal tissues ( n = 6) and tumor tissues ( n = 12) were measured by qPCR and western blotting, respectively. F Representative immunohistochemical staining of normal tissues ( n = 13) and tumor tissues ( n = 13) with LPCAT1 antibody. Scale bar, 200 μm in the upper image, 50 μm in the lower image. Statistical significance determined by Mann–Whitney test ( B , F ) or Student’s t test ( A ) or Student’s t test with Welch’s correction ( D , E ). * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

Article Snippet: B LPCAT1 protein expression in HNSCC ( n = 109) and normal tissues ( n = 70) from the Human Protein Atlas database.

Techniques: Expressing, Immunohistochemical staining, Staining, Western Blot, MANN-WHITNEY

Journal: Cell Death Discovery

Article Title: Lysophosphatidylcholine acyltransferase 1 promotes head and neck squamous cell carcinoma progression by enhancing COX17-dependent oxidative phosphorylation

doi: 10.1038/s41420-026-02994-3

Figure Lengend Snippet: A CCK-8 assay was used to detect the proliferation of FaDu and SCC15 cells with or without LPCAT1 knockdown. B Cell growth was measured by colony formation assays. C Live/Dead staining for cell death measurement. Representative images were shown. Green represents live cells, and red represents dead cells. The percentage of dead cells was calculated. Scale bar, 50 μm. D Wound-healing assays were used to detect cell migration. Scale bar, 200 μm. E Transwell invasion assays were used to evaluate cell invasive potential. Scale bar, 50 μm, n = 3 for ( A – E ). Statistical significance determined by Student’s t test ( B – E ) or two-way ANOVA ( A ). * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

Article Snippet: B LPCAT1 protein expression in HNSCC ( n = 109) and normal tissues ( n = 70) from the Human Protein Atlas database.

Techniques: CCK-8 Assay, Knockdown, Staining, Migration

Journal: Cell Death Discovery

Article Title: Lysophosphatidylcholine acyltransferase 1 promotes head and neck squamous cell carcinoma progression by enhancing COX17-dependent oxidative phosphorylation

doi: 10.1038/s41420-026-02994-3

Figure Lengend Snippet: A Body weight was monitored every 5 days for 15 days after tumor implantation. B In vivo fluorescence imaging of FaDu-shLPCAT1 and FaDu-shNC xenografts at endpoint and quantification of fluorescence intensity from endpoint imaging. C Tumor volume was measured at the endpoint. Statistical analysis of tumor volume differences between the LPCAT1 knockdown and control groups. D Representative immunohistochemical staining of tumor tissues from FaDu-shLPCAT1 and FaDu-shNC xenografts with Ki-67 antibody. Scale bar, 50 μm. The percentages of Ki-67-positive cells were calculated. Statistical significance determined by Student’s t test ( C ), Student’s t test with Welch’s correction ( D ), Mann–Whitney test ( B ). * P < 0.05.

Article Snippet: B LPCAT1 protein expression in HNSCC ( n = 109) and normal tissues ( n = 70) from the Human Protein Atlas database.

Techniques: Tumor Implantation, In Vivo, Fluorescence, Imaging, Knockdown, Control, Immunohistochemical staining, Staining, MANN-WHITNEY

Journal: Cell Death Discovery

Article Title: Lysophosphatidylcholine acyltransferase 1 promotes head and neck squamous cell carcinoma progression by enhancing COX17-dependent oxidative phosphorylation

doi: 10.1038/s41420-026-02994-3

Figure Lengend Snippet: A CCK-8 assay was used to monitor cell proliferation. B Volcano plot of differentially expressed genes (DEGs) in LPCAT1-knockdown (FaDu-siLPCAT1) and control (FaDu-siNC) cells, showing 622 downregulated and 371 upregulated genes (fold change ≥2, P adjust <0.05). C KEGG pathway enrichment analysis of differentially expressed genes ( P adjust <0.01). D Differential pathway activity between LPCAT1-knockdown and control groups ( P adjust <0.05). E Heatmaps displaying GSVA enrichment scores per sample for each module. F The mitochondrial membrane potential was detected by JC-1 staining. Images were acquired using fluorescence microscopy. Scale bar, 50 μm. G ATP production was measured by quantification assays following LPCAT1 knockdown or overexpression. Mitochondrial respiration was measured by Seahorse XFp analysis following LPCAT1 knockdown ( H ) or overexpression ( I ). Basal OCR, ATP-linked OCR, and maximal OCR were calculated. n = 3 for ( A – I ). Statistical significance determined by two-way ANOVA test ( A ), or one-way ANOVA with Dunnett’s multiple comparison test ( F ), or Student’s t test ( G – I ). * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: B LPCAT1 protein expression in HNSCC ( n = 109) and normal tissues ( n = 70) from the Human Protein Atlas database.

Techniques: CCK-8 Assay, Knockdown, Control, Activity Assay, Membrane, Staining, Fluorescence, Microscopy, Over Expression, Comparison

Journal: Cell Death Discovery

Article Title: Lysophosphatidylcholine acyltransferase 1 promotes head and neck squamous cell carcinoma progression by enhancing COX17-dependent oxidative phosphorylation

doi: 10.1038/s41420-026-02994-3

Figure Lengend Snippet: A Transcriptomic analysis highlighting COX17 as a top dysregulated gene within the oxidative phosphorylation pathway in LPCAT1-knockdown cells. B qPCR validation of top 10 genes mRNA levels. C COX17 protein was measured by western blotting after LPCAT1 knockdown or overexpression. Representative immunofluorescence staining of LPCAT1 knockdown ( D ) or overexpression ( E ) cells with mitotracker and COX17 antibody. Images were acquired using confocal microscopy. Scale bar, 20 μm. F Western blotting analysis of electron transport chain complex core subunits (I–V). G Microplate assay measurement of CcO activity after LPCAT1 knockdown or overexpression. Overexpress COX17 in LPCAT1-knockdown cells or silence COX17 in LPCAT1-overexpression cells. CcO activity ( H ) and ATP production ( I ) were detected, and a mitochondrial stress test ( J ) was performed. K The potential transcription factors of COX17 were predicted by Genecard, KnockTF, and hTFtarget. Representative immunofluorescence staining of LPCAT1 knockdown ( L ) or overexpression ( M ) cells with SP1 antibody. Scale, 20 μm. SP1 in the nucleus is indicated by white arrows. n = 3 for ( B – J , L , M ). Statistical significance determined by Student’s t test ( B , C , F , G ), or one-way ANOVA with Tukey’s multiple comparison test ( H – J ). * P < 0.05; ** P < 0.01, *** P < 0.001, # P < 0.05, ## P <0.01.

Article Snippet: B LPCAT1 protein expression in HNSCC ( n = 109) and normal tissues ( n = 70) from the Human Protein Atlas database.

Techniques: Phospho-proteomics, Knockdown, Biomarker Discovery, Western Blot, Over Expression, Immunofluorescence, Staining, Confocal Microscopy, Activity Assay, Comparison