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loopseqtm 16s long read kit  (Loop Genomics)

 
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    Loop Genomics loopseqtm 16s long read kit
    Loopseqtm 16s Long Read Kit, supplied by Loop Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/loopseqtm+16s+long+read+kit/loopseq+pcr+amplicon+24+sample+kit/pmc10800392-91-1-6
    Average 90 stars, based on 1 article reviews
    loopseqtm 16s long read kit - by Bioz Stars, 2026-09
    90/100 stars

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    Amplification:

    Article Title: Soil quality and microbial communities in subtropical slope lands under different agricultural management practices
    Article Snippet: Purified DNA was quantitated using the Qubit dsDNA HS assay (ThermoFisher Scientific, San Jose, CA, USA), and qualitated using the Agilent 4200 TapeStation system (Agilent Technologies, Palo Alto, CA, USA). .. The LoopSeqTM 16S Long Read Kit (Loop Genomics, San Hose, CA, USA) was used to generate the full-length 16S rDNA amplicon library. .. In particular, V1 to V9 regions were amplified by specific primers (27F: 5′-AGAGTTTGATCMTGGCTCAG-3′, 1492R: 5′-TACCTTGTTACGACTT-3′) and sequenced on an Illumina NovaSeq platform (Illumina, San Diego, CA, USA) using a paired-end 150 bp mode.



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    Overview of the experimental procedure. Mock communities of known composition and bovine raw milk samples were used for the microbial gDNA extraction (grey above). The samples were prepared for short amplicon <t>16S</t> rRNA gene sequencing (blue) and full-length sequencing using the <t>16S/18S</t> kit targeting the SSU rRNA (orange). After the libraries were prepared, cleaned, and attested to be of good quality, the sequencing was performed on an Illumina MiSeq (grey, below). The execution time estimations in hours are shown for all steps.
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    Overview of the experimental procedure. Mock communities of known composition and bovine raw milk samples were used for the microbial gDNA extraction (grey above). The samples were prepared for short amplicon <t>16S</t> rRNA gene sequencing (blue) and full-length sequencing using the <t>16S/18S</t> kit targeting the SSU rRNA (orange). After the libraries were prepared, cleaned, and attested to be of good quality, the sequencing was performed on an Illumina MiSeq (grey, below). The execution time estimations in hours are shown for all steps.
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    Overview of the experimental procedure. Mock communities of known composition and bovine raw milk samples were used for the microbial gDNA extraction (grey above). The samples were prepared for short amplicon 16S rRNA gene sequencing (blue) and full-length sequencing using the 16S/18S kit targeting the SSU rRNA (orange). After the libraries were prepared, cleaned, and attested to be of good quality, the sequencing was performed on an Illumina MiSeq (grey, below). The execution time estimations in hours are shown for all steps.

    Journal: Microorganisms

    Article Title: Full-Length SSU rRNA Gene Sequencing Allows Species-Level Detection of Bacteria, Archaea, and Yeasts Present in Milk

    doi: 10.3390/microorganisms9061251

    Figure Lengend Snippet: Overview of the experimental procedure. Mock communities of known composition and bovine raw milk samples were used for the microbial gDNA extraction (grey above). The samples were prepared for short amplicon 16S rRNA gene sequencing (blue) and full-length sequencing using the 16S/18S kit targeting the SSU rRNA (orange). After the libraries were prepared, cleaned, and attested to be of good quality, the sequencing was performed on an Illumina MiSeq (grey, below). The execution time estimations in hours are shown for all steps.

    Article Snippet: On the other hand, the samples were processed by using the LoopSeqTM 16S & 18S Long Read Kit (Loop Genomics, San Jose, California, USA, highlighted orange in ).

    Techniques: Extraction, Amplification, Sequencing

    Relative abundance (%) at the kingdom and genus levels after the 16S/18S rRNA (SSU) gene sequencing analysis using the LoopGenomics kit. Taxonomic classification regarding the kingdom ( left ) and genus-level of the top 50 taxa ( right ) of each LoopSeq-sequenced milk sample. All of the other remaining taxa are shown in hatched blue.

    Journal: Microorganisms

    Article Title: Full-Length SSU rRNA Gene Sequencing Allows Species-Level Detection of Bacteria, Archaea, and Yeasts Present in Milk

    doi: 10.3390/microorganisms9061251

    Figure Lengend Snippet: Relative abundance (%) at the kingdom and genus levels after the 16S/18S rRNA (SSU) gene sequencing analysis using the LoopGenomics kit. Taxonomic classification regarding the kingdom ( left ) and genus-level of the top 50 taxa ( right ) of each LoopSeq-sequenced milk sample. All of the other remaining taxa are shown in hatched blue.

    Article Snippet: On the other hand, the samples were processed by using the LoopSeqTM 16S & 18S Long Read Kit (Loop Genomics, San Jose, California, USA, highlighted orange in ).

    Techniques: Sequencing

    Overview of the experimental procedure. Mock communities of known composition and bovine raw milk samples were used for the microbial gDNA extraction (grey above). The samples were prepared for short amplicon 16S rRNA gene sequencing (blue) and full-length sequencing using the 16S/18S kit targeting the SSU rRNA (orange). After the libraries were prepared, cleaned, and attested to be of good quality, the sequencing was performed on an Illumina MiSeq (grey, below). The execution time estimations in hours are shown for all steps.

    Journal: Microorganisms

    Article Title: Full-Length SSU rRNA Gene Sequencing Allows Species-Level Detection of Bacteria, Archaea, and Yeasts Present in Milk

    doi: 10.3390/microorganisms9061251

    Figure Lengend Snippet: Overview of the experimental procedure. Mock communities of known composition and bovine raw milk samples were used for the microbial gDNA extraction (grey above). The samples were prepared for short amplicon 16S rRNA gene sequencing (blue) and full-length sequencing using the 16S/18S kit targeting the SSU rRNA (orange). After the libraries were prepared, cleaned, and attested to be of good quality, the sequencing was performed on an Illumina MiSeq (grey, below). The execution time estimations in hours are shown for all steps.

    Article Snippet: The library preparation was performed as described by LoopGenomics for the LoopSeqTM 16S & 18S Long Read Kit (Version 2.1, Loop Genomics, San Jose, CA, USA).

    Techniques: Extraction, Amplification, Sequencing

    Relative abundance (%) at the kingdom and genus levels after the 16S/18S rRNA (SSU) gene sequencing analysis using the LoopGenomics kit. Taxonomic classification regarding the kingdom ( left ) and genus-level of the top 50 taxa ( right ) of each LoopSeq-sequenced milk sample. All of the other remaining taxa are shown in hatched blue.

    Journal: Microorganisms

    Article Title: Full-Length SSU rRNA Gene Sequencing Allows Species-Level Detection of Bacteria, Archaea, and Yeasts Present in Milk

    doi: 10.3390/microorganisms9061251

    Figure Lengend Snippet: Relative abundance (%) at the kingdom and genus levels after the 16S/18S rRNA (SSU) gene sequencing analysis using the LoopGenomics kit. Taxonomic classification regarding the kingdom ( left ) and genus-level of the top 50 taxa ( right ) of each LoopSeq-sequenced milk sample. All of the other remaining taxa are shown in hatched blue.

    Article Snippet: The library preparation was performed as described by LoopGenomics for the LoopSeqTM 16S & 18S Long Read Kit (Version 2.1, Loop Genomics, San Jose, CA, USA).

    Techniques: Sequencing