Journal: Microorganisms

Article Title: Full-Length SSU rRNA Gene Sequencing Allows Species-Level Detection of Bacteria, Archaea, and Yeasts Present in Milk

doi: 10.3390/microorganisms9061251

Figure Lengend Snippet: Overview of the experimental procedure. Mock communities of known composition and bovine raw milk samples were used for the microbial gDNA extraction (grey above). The samples were prepared for short amplicon 16S rRNA gene sequencing (blue) and full-length sequencing using the 16S/18S kit targeting the SSU rRNA (orange). After the libraries were prepared, cleaned, and attested to be of good quality, the sequencing was performed on an Illumina MiSeq (grey, below). The execution time estimations in hours are shown for all steps.

Article Snippet: On the other hand, the samples were processed by using the LoopSeqTM 16S & 18S Long Read Kit (Loop Genomics, San Jose, California, USA, highlighted orange in ).

Techniques: Extraction, Amplification, Sequencing

Journal: Microorganisms

Article Title: Full-Length SSU rRNA Gene Sequencing Allows Species-Level Detection of Bacteria, Archaea, and Yeasts Present in Milk

doi: 10.3390/microorganisms9061251

Figure Lengend Snippet: Relative abundance (%) at the kingdom and genus levels after the 16S/18S rRNA (SSU) gene sequencing analysis using the LoopGenomics kit. Taxonomic classification regarding the kingdom ( left ) and genus-level of the top 50 taxa ( right ) of each LoopSeq-sequenced milk sample. All of the other remaining taxa are shown in hatched blue.

Article Snippet: On the other hand, the samples were processed by using the LoopSeqTM 16S & 18S Long Read Kit (Loop Genomics, San Jose, California, USA, highlighted orange in ).

Techniques: Sequencing

Journal: Microorganisms

Article Title: Full-Length SSU rRNA Gene Sequencing Allows Species-Level Detection of Bacteria, Archaea, and Yeasts Present in Milk

doi: 10.3390/microorganisms9061251

Figure Lengend Snippet: Overview of the experimental procedure. Mock communities of known composition and bovine raw milk samples were used for the microbial gDNA extraction (grey above). The samples were prepared for short amplicon 16S rRNA gene sequencing (blue) and full-length sequencing using the 16S/18S kit targeting the SSU rRNA (orange). After the libraries were prepared, cleaned, and attested to be of good quality, the sequencing was performed on an Illumina MiSeq (grey, below). The execution time estimations in hours are shown for all steps.

Article Snippet: The library preparation was performed as described by LoopGenomics for the LoopSeqTM 16S & 18S Long Read Kit (Version 2.1, Loop Genomics, San Jose, CA, USA).

Techniques: Extraction, Amplification, Sequencing

Journal: Microorganisms

Article Title: Full-Length SSU rRNA Gene Sequencing Allows Species-Level Detection of Bacteria, Archaea, and Yeasts Present in Milk

doi: 10.3390/microorganisms9061251

Figure Lengend Snippet: Relative abundance (%) at the kingdom and genus levels after the 16S/18S rRNA (SSU) gene sequencing analysis using the LoopGenomics kit. Taxonomic classification regarding the kingdom ( left ) and genus-level of the top 50 taxa ( right ) of each LoopSeq-sequenced milk sample. All of the other remaining taxa are shown in hatched blue.

Article Snippet: The library preparation was performed as described by LoopGenomics for the LoopSeqTM 16S & 18S Long Read Kit (Version 2.1, Loop Genomics, San Jose, CA, USA).

Techniques: Sequencing