ln18  (ATCC)

Journal: Molecular Therapy Oncology

Article Title: Potency-enhancing mutations in E3-19K and i-leader increase the cytolytic activity of the PH20/ SPAM1 -armed oncolytic adenovirus Ad5Δ24RGD

doi: 10.1016/j.omton.2026.201137

Figure Lengend Snippet: The 19K SS and iL Q125Ter modifications mutually increase the potency of OAds regardless of the type of fiber modification (A) Sizes of MTT-stained plaques in cancer cell monolayers under an agarose overlay. Two to three independent experiments were conducted. The total number ( n ) of analyzed plaques is indicated for each group. Data are shown as means (SD) and analyzed using Brown-Forsythe and Welch ANOVA tests with Games-Howell post hoc multiple comparisons. (B) IC 50 values derived from viability curves after infection of cells (2.5 × 10 4 per well) in suspension with serial 3-fold dilutions of the indicated OAds. Data were collected on day 6 post-infection for all cell cultures except LN18 and LN229 cells infected with Ad5Δ24RGD-derived OAds (on day 7). Two to three independent experiments with technical triplicates were performed. Data are shown as means (SD). Only ≥3-fold differences from the parental virus Ad5Δ24RGD are indicated. (C) Sizes of fluorescent plaques and MTT-stained plaques in cancer cell monolayers under an agarose overlay. Three to four independent experiments were performed. The total number ( n ) of analyzed plaques is indicated for each group. Data are shown as means (SD) and analyzed using Brown-Forsythe and Welch ANOVA tests and Games-Howell post hoc multiple comparisons. ∗∗∗∗ p ≤ 0.0001; ∗∗∗ p ≤ 0.001; ∗∗ p ≤ 0.01; ∗ p ≤ 0.05; NS, not significant. See also .

Article Snippet: Human embryonic kidney HEK293, lung adenocarcinoma A549 and H441, prostate adenocarcinoma LNCap and PC3, hepatocellular carcinoma HepG2 and Hep3B, glioblastoma LN18, LN229, DBTRG, T98G, and U87 (American Type Culture Collection, ATCC), murine glioma CT-2A (#SCC194, Sigma) and GL261 cells (#ACC802, DSMZ Cell Culture Collection), and human short-term glioma (III–IV grade) primary cell cultures (GliSav, GliShat, GliBah, GliDu, AG-AASH, GBM100622, and GBM160323) were grown in Dulbecco’s modified Eagle medium (DMEM) with L-alanyl-glutamine, 4.5 g/L glucose, and Na pyruvate (#C415, Paneco), supplemented with 10% fetal bovine serum (FBS; HyClone/Cytiva, neoFroxx, or Capricorn Scientific) and penicillin-streptomycin solution at a final concentration of 50 U/mL and 50 μg/mL (#А063, Paneco).

Techniques: Modification, Staining, Derivative Assay, Infection, Suspension, Virus

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Dasatinib boosts γδ T cell expansion and memory phenotypes with enhanced antitumor immunity

doi: 10.1007/s00262-026-04335-w

Figure Lengend Snippet: Phenotypic characterization of ex vivo expanded γδ2 T cells. A Overview of Zol-based γδ2 T cell expansion from human PBMCs. B – C Time-course analysis of γδ2 cell population dynamics from five healthy donors (HD-1 to HD-5), showing initial decline followed by robust expansion. The blue line indicates the poor expander (HD-3 and HD-5), whose γδ2 T cells constitute less than 60% of all CD3 + cells. D t-SNE and FlowSOM clustering identified nine phenotypically distinct CD3 + VD2 + -γδ2 T cell subsets (P0–P8) during cell expansion based on the expression of surface markers from the pool sample of five healthy donors. E Marker expression heatmap defines γδ2 T cell subsets (P0–P8). F Histogram analysis of key memory and cytotoxic markers over culture duration, data from the pool sample of five healthy donors. G Cytotoxicity of γδ2 T cells from HD-1 against Ln18 cells decreases with extended culture, as measured by co-culture apoptosis assays

Article Snippet: Human glioblastoma cell lines Ln18 were purchased from the American Type Culture Collection (Manassas, VA); human glioblastoma cell lines GBM8901 and human breast adenocarcinoma cell line MDA-MB231 were purchased from Bioresources Collection and Research Center (Food Industry Research and Development Institute, Hsinchu, Taiwan).

Techniques: Ex Vivo, Expressing, Marker, Co-Culture Assay

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Dasatinib boosts γδ T cell expansion and memory phenotypes with enhanced antitumor immunity

doi: 10.1007/s00262-026-04335-w

Figure Lengend Snippet: Dasatinib-cultured γδ2 T cells exhibit superior in vitro antitumor activity and persistence. A Cytotoxicity of γδ2 T cells expanded with dasatinib (γδ2 T-Da) or vehicle (γδ2 T-Ve) against MDA-MB-231 (TNBC), Ln18, and GBM8901 (GBM) cancer cells in a 5-h co-culture assay. Tumor cell apoptosis was measured by Annexin V positivity. While γδ2 T-Ve cells showed donor-variable cytotoxicity, γδ2 T-Da cells consistently exhibited enhanced tumor cell killing in a majority of tested donors. B Polyfunctional cytokine profiling of γδ2 T cells post co-culture, assessed by intracellular staining for TNF-α, IFN-γ, IL2, IL4, and IL17A. γδ2 T-Da cells displayed higher proportions of TNF-α⁺ and IFN-γ⁺ cells, indicating a more robust effector response. C Long-term cytotoxicity against GBM8901 cells using γδ2 T-Da and γδ2 T-Ve cells harvested at day 7, 10, and 13 of ex vivo expansion. γδ2 T-Da cells retained greater cytotoxicity over time, whereas γδ2 T-Ve cells showed a progressive loss of killing ability. D Serial killing assay demonstrating that γδ2 T-Da cells maintain cytotoxic function over repeated tumor cell challenges, suggesting enhanced persistence and durability of antitumor activity. Student t -test is used, * p < 0.05, ** p < 0.01 and *** p < 0.001. The effector-to-target (E:T) ratios used in A , C , and D were 1:1

Article Snippet: Human glioblastoma cell lines Ln18 were purchased from the American Type Culture Collection (Manassas, VA); human glioblastoma cell lines GBM8901 and human breast adenocarcinoma cell line MDA-MB231 were purchased from Bioresources Collection and Research Center (Food Industry Research and Development Institute, Hsinchu, Taiwan).

Techniques: Cell Culture, In Vitro, Activity Assay, Co-culture Assay, Co-Culture Assay, Staining, Ex Vivo