ll37 solution  (MedChemExpress)

Journal: ImmunoTargets and Therapy

Article Title: UVB Upregulates Inflammatory Cytokines in Rosacea Cell Model by Promoting the Expression of TRPVs and TLR2

doi: 10.2147/ITT.S571037

Figure Lengend Snippet: The effect of UVB irradiation on the secretion of inflammatory cytokines in the rosacea keratinocyte model. ( A ) The proliferation activity of HaCaT cells at 0 μM, 2 μM, 4 μM, 6 μM, and 8 μM LL-37 was detected by the CCK-8 method (n = 6). ( B ) The microscopic morphology of HaCaT cells at 0 μM, 2 μM, 4 μM, 6 μM, and 8 μM LL-37 under a 40× microscope. ( C ) The secretion levels of inflammatory factors in HaCaT cells stimulated by 0 μM, 2 μM, 4 μM, 6 μM, and 8 μM LL-37 were detected by ELISA (n = 6, *P < 0.05, *P < 0.01, ***P < 0.001, ****P < 0.0001). ( D ) The secretion levels of rosacea-related inflammatory factors in HaCaT cells after induction by 8 μM LL-37 and/or 25 mJ/cm² UVB were detected by ELISA (n = 3, compared with the control group, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; compared with the LL-37 + UVB group, # P < 0.05, ### P < 0.001, #### P < 0.0001).

Article Snippet: The rosacea model of HaCaT cells was induced through treatment with LL-37 (Selleck, China).

Techniques: Irradiation, Activity Assay, CCK-8 Assay, Microscopy, Enzyme-linked Immunosorbent Assay, Control

Journal: ImmunoTargets and Therapy

Article Title: UVB Upregulates Inflammatory Cytokines in Rosacea Cell Model by Promoting the Expression of TRPVs and TLR2

doi: 10.2147/ITT.S571037

Figure Lengend Snippet: The influence of UVB on the mRNA and protein expressions of TRPV1, TRPV2, TRPV3, TRPV4 and TLR2 in the rosacea keratinocyte model. ( A ) The expression levels of TRPV1, TRPV2, TRPV3, TRPV4 and TLR2 mRNA in HaCaT cells and HaCaT cells treated by 8 μM LL-37 after 25 mJ/cm² UVB exposure were detected by qRT-PCR. ( B and C ) The protein expression levels of TRPV1, TRPV2, TRPV3, TRPV4 and TLR2 in HaCaT cells and HaCaT cells stimulated by 8 μM LL-37 after 25 mJ/cm² UVB irradiation were measured by Western blot. (n = 3, compared with the control group, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; compared with the LL-37 + UVB group, ## P < 0.01, #### P < 0.0001.).

Article Snippet: The rosacea model of HaCaT cells was induced through treatment with LL-37 (Selleck, China).

Techniques: Expressing, Quantitative RT-PCR, Irradiation, Western Blot, Control

Journal: Communications Biology

Article Title: Transcriptomic and metabolomic insights into gabapentin’s therapeutic role in neurogenic inflammation of rosacea

doi: 10.1038/s42003-026-09662-3

Figure Lengend Snippet: A Schematic representation of establishing a mouse model of rosacea-like skin inflammation using LL37 and treatment with GBP (Created by BioRender); B Administration of GBP to different groups of mice and observation of skin symptoms at the end of the final experiment; C Assessment of each group of mice’s skin thickness; D Evaluation of skin redness score in each group of mice; E Overall skin condition score for each group of mice; F Histopathological assessment using H&E staining to examine skin conditions of each group of mice, with a scale bar of 100 μm and a local magnification scale bar of 25 μm; G Analysis of inflammatory cell infiltration in different groups. All data are expressed as mean ± standard deviation (SD). Comparisons among multiple groups were performed using one-way analysis of variance (ANOVA), followed by post-hoc tests with Dunnett’s T3 and LSD-t methods. For animal experiments, n = 5. Statistical significance was defined as ns P > 0.05; *** P < 0.001.

Article Snippet: Anesthetized with pentobarbital sodium (50 mg/kg), the mice were subcutaneously injected with 40 μL of 320 μM LL37 (HY-P1222, MCE) twice a day for two days, wild-type mice were used as controls.

Techniques: Staining, Standard Deviation

Journal: Communications Biology

Article Title: Transcriptomic and metabolomic insights into gabapentin’s therapeutic role in neurogenic inflammation of rosacea

doi: 10.1038/s42003-026-09662-3

Figure Lengend Snippet: A Collection of skin tissue from various groups of mice for transcriptome high-throughput sequencing (Created by BioRender); B Volcano plot analysis of DEGs between the Ctrl group and LL37 group from high-throughput transcriptome sequencing, where blue dots represent downregulated genes, red dots depict upregulated genes, and gray dots indicate insignificant genes, n = 3; C Differential gene analysis between LL37 and GBP groups by high-throughput transcriptome sequencing, with blue dots representing downregulated genes, red dots indicating upregulated genes, and gray dots showing insignificant genes, n = 3; D PCA analysis of differential genes between Ctrl, LL37, and GBP groups; E Venn diagram analysis of differential genes between Ctrl, LL37, and GBP groups, with blue circle representing genes differentially expressed in Ctrl and LL37 groups, and red circle indicating genes differentially expressed in LL37 and GBP groups; F Heat map showing the expression of selected inflammatory factors in the intersecting genes.

Article Snippet: Anesthetized with pentobarbital sodium (50 mg/kg), the mice were subcutaneously injected with 40 μL of 320 μM LL37 (HY-P1222, MCE) twice a day for two days, wild-type mice were used as controls.

Techniques: Next-Generation Sequencing, High Throughput Screening Assay, Sequencing, Expressing

Journal: Communications Biology

Article Title: Transcriptomic and metabolomic insights into gabapentin’s therapeutic role in neurogenic inflammation of rosacea

doi: 10.1038/s42003-026-09662-3

Figure Lengend Snippet: A KEGG and GO analyses reveal the upregulated DEGs controlling biological pathways in the LL37 and GBP groups; B KEGG and GO analyses show the downregulated DEGs controlling biological pathways in the LL37 and GBP groups; C , D GSEA analysis indicates the expression pattern of inflammatory factors regulated by Neuroinflammation and Glutamatergic Signaling; E , F GSEA analysis shows the expression pattern of inflammatory factors regulated by the NF-κB Signaling pathway.

Article Snippet: Anesthetized with pentobarbital sodium (50 mg/kg), the mice were subcutaneously injected with 40 μL of 320 μM LL37 (HY-P1222, MCE) twice a day for two days, wild-type mice were used as controls.

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Risk Factors Associated with the Development of Thrombotic Microangiopathy in Patients with Dermatomyositis

doi: 10.3390/ijms27010315

Figure Lengend Snippet: Representative immunofluorescence confocal image of a muscle biopsy showing neutrophil extracellular traps (NETs) infiltrating the muscle tissue of dermatomyositis patient. Blue represents DNA (Column 1a , 2a , 3a ), green represents citrullinated histone H3 ( 1b , 2b , 3b ), and red represents LL37 ( 1c , 2c , 3c ). Right column images depict merged images for each tissue ( 1d , 2d , 3d ). Original magnification, ×10. Scale bar 100 µm in the first two rows and 50 µm in the third one.

Article Snippet: Then, the slides were incubated overnight at 4 °C with rabbit anti–human citrullinated histone H3 (Abcam, Cambridge, UK; dilution 1:750), and mouse anti–human LL37 (Santa Cruz Biotechnology, Dallas, TX, USA; 1: 100) diluted in 5% BSA.

Techniques: Immunofluorescence