rabbit anti lifr (Proteintech)
Structured Review
Rabbit Anti Lifr, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lifr/LIFR+Antibody/pmc12994253-129-35-38
Average 92 stars, based on 30 article reviews
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Extraction:Article Title: miR-196a-5p promotes the proliferation, migration, and invasion of esophageal squamous cell carcinoma by targeting LIFR. Article Snippet: .. Total protein was extracted with a RIPA extraction reagent (Beyotime, Shanghai, China), and the proteins were separated through 10% SDS-PAGE gel electrophoresis, transferred to PVDF membranes, and blocked with 5% skimmed milk powder on a 37 °C shaker for 1 h, washed three times with TBST buffer, and incubated with primary antibodies E-cadherin (1:2000; 20874-1-AP; Proteintech, Wuhan, China), N-cadherin (1:2000; 22018- 1-AP; Proteintech), Vimentin (1:10000; 10366-1-AP; Proteintech), Article Title: miR-196a-5p promotes the proliferation, migration, and invasion of esophageal squamous cell carcinoma by targeting LIFR Article Snippet: .. Total protein was extracted with a RIPA extraction reagent (Beyotime, Shanghai, China), and the proteins were separated through 10% SDS-PAGE gel electrophoresis, transferred to PVDF membranes, and blocked with 5% skimmed milk powder on a 37 °C shaker for 1 h, washed three times with TBST buffer, and incubated with primary antibodies E-cadherin (1:2000; 20874-1-AP; Proteintech, Wuhan, China), N-cadherin (1:2000; 22018-1-AP; Proteintech), Vimentin (1:10000; 10366-1-AP; Proteintech), SDS Page:Article Title: miR-196a-5p promotes the proliferation, migration, and invasion of esophageal squamous cell carcinoma by targeting LIFR. Article Snippet: .. Total protein was extracted with a RIPA extraction reagent (Beyotime, Shanghai, China), and the proteins were separated through 10% SDS-PAGE gel electrophoresis, transferred to PVDF membranes, and blocked with 5% skimmed milk powder on a 37 °C shaker for 1 h, washed three times with TBST buffer, and incubated with primary antibodies E-cadherin (1:2000; 20874-1-AP; Proteintech, Wuhan, China), N-cadherin (1:2000; 22018- 1-AP; Proteintech), Vimentin (1:10000; 10366-1-AP; Proteintech), Article Title: miR-196a-5p promotes the proliferation, migration, and invasion of esophageal squamous cell carcinoma by targeting LIFR Article Snippet: .. Total protein was extracted with a RIPA extraction reagent (Beyotime, Shanghai, China), and the proteins were separated through 10% SDS-PAGE gel electrophoresis, transferred to PVDF membranes, and blocked with 5% skimmed milk powder on a 37 °C shaker for 1 h, washed three times with TBST buffer, and incubated with primary antibodies E-cadherin (1:2000; 20874-1-AP; Proteintech, Wuhan, China), N-cadherin (1:2000; 22018-1-AP; Proteintech), Vimentin (1:10000; 10366-1-AP; Proteintech), Nucleic Acid Electrophoresis:Article Title: miR-196a-5p promotes the proliferation, migration, and invasion of esophageal squamous cell carcinoma by targeting LIFR. Article Snippet: .. Total protein was extracted with a RIPA extraction reagent (Beyotime, Shanghai, China), and the proteins were separated through 10% SDS-PAGE gel electrophoresis, transferred to PVDF membranes, and blocked with 5% skimmed milk powder on a 37 °C shaker for 1 h, washed three times with TBST buffer, and incubated with primary antibodies E-cadherin (1:2000; 20874-1-AP; Proteintech, Wuhan, China), N-cadherin (1:2000; 22018- 1-AP; Proteintech), Vimentin (1:10000; 10366-1-AP; Proteintech), Article Title: miR-196a-5p promotes the proliferation, migration, and invasion of esophageal squamous cell carcinoma by targeting LIFR Article Snippet: .. Total protein was extracted with a RIPA extraction reagent (Beyotime, Shanghai, China), and the proteins were separated through 10% SDS-PAGE gel electrophoresis, transferred to PVDF membranes, and blocked with 5% skimmed milk powder on a 37 °C shaker for 1 h, washed three times with TBST buffer, and incubated with primary antibodies E-cadherin (1:2000; 20874-1-AP; Proteintech, Wuhan, China), N-cadherin (1:2000; 22018-1-AP; Proteintech), Vimentin (1:10000; 10366-1-AP; Proteintech), Incubation:Article Title: miR-196a-5p promotes the proliferation, migration, and invasion of esophageal squamous cell carcinoma by targeting LIFR. Article Snippet: .. Total protein was extracted with a RIPA extraction reagent (Beyotime, Shanghai, China), and the proteins were separated through 10% SDS-PAGE gel electrophoresis, transferred to PVDF membranes, and blocked with 5% skimmed milk powder on a 37 °C shaker for 1 h, washed three times with TBST buffer, and incubated with primary antibodies E-cadherin (1:2000; 20874-1-AP; Proteintech, Wuhan, China), N-cadherin (1:2000; 22018- 1-AP; Proteintech), Vimentin (1:10000; 10366-1-AP; Proteintech), Article Title: miR-196a-5p promotes the proliferation, migration, and invasion of esophageal squamous cell carcinoma by targeting LIFR Article Snippet: .. Total protein was extracted with a RIPA extraction reagent (Beyotime, Shanghai, China), and the proteins were separated through 10% SDS-PAGE gel electrophoresis, transferred to PVDF membranes, and blocked with 5% skimmed milk powder on a 37 °C shaker for 1 h, washed three times with TBST buffer, and incubated with primary antibodies E-cadherin (1:2000; 20874-1-AP; Proteintech, Wuhan, China), N-cadherin (1:2000; 22018-1-AP; Proteintech), Vimentin (1:10000; 10366-1-AP; Proteintech), Article Title: Distinct adipose progenitor cells emerging with age drive active adipogenesis. Article Snippet: INTRODUCTION: Adipose tissue plays a crucial role in regulating various hormonal andmetabolic processes and demonstrates substantial compositional and phenotypic plasticity.. From middle age to early aging, adults often experience a notable increase in visceral adipose tissuemass.. Visceral adiposity is believed to be an important risk factor for various metabolic disorders. Article Title: miR-196a-5p promotes the proliferation, migration, and invasion of esophageal squamous cell carcinoma by targeting LIFR Article Snippet: .. Endogenous peroxidase activity was blocked with 3% H 2 O 2 for 10 min, and antigenicity was determined after boiling in EDTA buffer (pH = 9) for 20 min. After incubation with bovine serum albumin for 20 min at room temperature, the primary antibody E-cadherin (1:200; 20874-1-AP; Proteintech), Ki67 (1:200; 27309-1-AP; Proteintech), Vimentin (1:200; 10366-1-AP; Proteintech), Article Title: Genome-wide analysis reveals porcine LIFR regulated by DNA methylation promotes the implantation process via the STAT3 signaling. Article Snippet: Embryo-uterine interaction during embryo implantation depends on the coordinated expression of numerous genes in the receptive endometrium.. While DNA methylation is known to play a significant role in controlling gene expression, specific molecular mechanisms underlying this regulatory event remain elusive in early porcine pregnancy.. Here, we investigated the genome-wide DNA methylation landscape in the Yorkshire and Meishan pig’s endometrium. Article Title: miR-196a-5p promotes the proliferation, migration, and invasion of esophageal squamous cell carcinoma by targeting LIFR. Article Snippet: .. Endogenous peroxidase activity was blocked with 3% H2O2 for 10 min, and antigenicity was determined after boiling in EDTA buffer (pH = 9) for 20 min. After incubation with bovine serum albumin for 20 min at room temperature, the primary antibody E-cadherin (1:200; 20874-1-AP; Proteintech), Ki67 (1:200; 27309-1-AP; Proteintech), Vimentin (1:200; 10366-1-AP; Proteintech), Immunohistochemistry:Article Title: Use of integrated spatial transcriptomics and histopathological analysis in adamantinomatous craniopharyngiomas to identify stromal cells as a new cellular source of leukemia inhibitory factor Article Snippet: © 2025 The authors, CC BY-NC-ND 4.0 (http://creativecommons.org/licenses/by-nc-nd/4.0/) ABBREVIATIONS ACP = adamantinomatous craniopharyngioma; AOD = average optical density; DEG = differentially expressed gene; GSEA = Gene Set Enrichment Analysis; H&E = hematoxylin and eosin; IL = interleukin; KEGG = Kyoto Encyclopedia of Genes and Genomes; LIF = leukemia inhibitory factor; LIFR = LIF receptor; OSM = oncostatin M; PE = palisade epithelium; SR = stellate reticulum.. SUBMITTED December 18, 2024.. ACCEPTED April 16, 2025. Immunofluorescence:Article Title: Use of integrated spatial transcriptomics and histopathological analysis in adamantinomatous craniopharyngiomas to identify stromal cells as a new cellular source of leukemia inhibitory factor Article Snippet: © 2025 The authors, CC BY-NC-ND 4.0 (http://creativecommons.org/licenses/by-nc-nd/4.0/) ABBREVIATIONS ACP = adamantinomatous craniopharyngioma; AOD = average optical density; DEG = differentially expressed gene; GSEA = Gene Set Enrichment Analysis; H&E = hematoxylin and eosin; IL = interleukin; KEGG = Kyoto Encyclopedia of Genes and Genomes; LIF = leukemia inhibitory factor; LIFR = LIF receptor; OSM = oncostatin M; PE = palisade epithelium; SR = stellate reticulum.. SUBMITTED December 18, 2024.. ACCEPTED April 16, 2025. Staining:Article Title: Use of integrated spatial transcriptomics and histopathological analysis in adamantinomatous craniopharyngiomas to identify stromal cells as a new cellular source of leukemia inhibitory factor Article Snippet: © 2025 The authors, CC BY-NC-ND 4.0 (http://creativecommons.org/licenses/by-nc-nd/4.0/) ABBREVIATIONS ACP = adamantinomatous craniopharyngioma; AOD = average optical density; DEG = differentially expressed gene; GSEA = Gene Set Enrichment Analysis; H&E = hematoxylin and eosin; IL = interleukin; KEGG = Kyoto Encyclopedia of Genes and Genomes; LIF = leukemia inhibitory factor; LIFR = LIF receptor; OSM = oncostatin M; PE = palisade epithelium; SR = stellate reticulum.. SUBMITTED December 18, 2024.. ACCEPTED April 16, 2025. Article Title: miR-196a-5p promotes the proliferation, migration, and invasion of esophageal squamous cell carcinoma by targeting LIFR Article Snippet: .. Endogenous peroxidase activity was blocked with 3% H 2 O 2 for 10 min, and antigenicity was determined after boiling in EDTA buffer (pH = 9) for 20 min. After incubation with bovine serum albumin for 20 min at room temperature, the primary antibody E-cadherin (1:200; 20874-1-AP; Proteintech), Ki67 (1:200; 27309-1-AP; Proteintech), Vimentin (1:200; 10366-1-AP; Proteintech), Article Title: miR-196a-5p promotes the proliferation, migration, and invasion of esophageal squamous cell carcinoma by targeting LIFR. Article Snippet: .. Endogenous peroxidase activity was blocked with 3% H2O2 for 10 min, and antigenicity was determined after boiling in EDTA buffer (pH = 9) for 20 min. After incubation with bovine serum albumin for 20 min at room temperature, the primary antibody E-cadherin (1:200; 20874-1-AP; Proteintech), Ki67 (1:200; 27309-1-AP; Proteintech), Vimentin (1:200; 10366-1-AP; Proteintech), Activity Assay:Article Title: miR-196a-5p promotes the proliferation, migration, and invasion of esophageal squamous cell carcinoma by targeting LIFR Article Snippet: .. Endogenous peroxidase activity was blocked with 3% H 2 O 2 for 10 min, and antigenicity was determined after boiling in EDTA buffer (pH = 9) for 20 min. After incubation with bovine serum albumin for 20 min at room temperature, the primary antibody E-cadherin (1:200; 20874-1-AP; Proteintech), Ki67 (1:200; 27309-1-AP; Proteintech), Vimentin (1:200; 10366-1-AP; Proteintech), Article Title: miR-196a-5p promotes the proliferation, migration, and invasion of esophageal squamous cell carcinoma by targeting LIFR. Article Snippet: .. Endogenous peroxidase activity was blocked with 3% H2O2 for 10 min, and antigenicity was determined after boiling in EDTA buffer (pH = 9) for 20 min. After incubation with bovine serum albumin for 20 min at room temperature, the primary antibody E-cadherin (1:200; 20874-1-AP; Proteintech), Ki67 (1:200; 27309-1-AP; Proteintech), Vimentin (1:200; 10366-1-AP; Proteintech), |

