100g gr etrumadenant  (MedChemExpress)

Journal: Scientific Reports

Article Title: Sophoricoside from sophora japonica L. is efficacious as monotherapy or in combination with lenvatinib in hepatocellular carcinoma via targeting EGFR

doi: 10.1038/s41598-025-33330-x

Figure Lengend Snippet: Lenvatinib-Sop combination synergistically suppresses survival, proliferation and induces apoptosis in HCC cells. (A and B) The IC50 of lenvatinib in Huh7 and SK-Hep-1 cells when administered alone or in combination with 75µM or 150µM sophoricoside, as assessed by CCK-8 assay. CI values for each dose were calculated using CompuSyn software. (C) The effect of lenvatinib, sophoricoside and combination therapy on cell viability in Huh7 and SK-Hep-1 cells was determined by CCK-8 assay. CCK-8 assay was used to investigate the effect of lenvatinib (Huh7: 1µM, SK-Hep-1: 6µM), sophoricoside (Huh7: 100µM, SK-Hep-1: 100µM) and combination (Huh7: 1 + 100µM, SK-Hep-1: 6 + 100µM) on the proliferation of Huh7 and SK-Hep-1 cells. (D) Colony formation assay was used to detect the effect of lenvatinib (Huh7: 1µM, SK-Hep-1: 6µM), sophoricoside (Huh7: 100µM, SK-Hep-1: 100µM) and combination (Huh7: 1 + 100µM, SK-Hep-1: 6 + 100µM) on the proliferation of Huh7 and SK-Hep-1 cells. (E) Huh7 and SK-Hep-1 cells were treated with lenvatinib (Huh7: 1µM, SK-Hep-1: 6µM), sophoricoside (Huh7: 100µM, SK-Hep-1: 100µM) and combination (Huh7: 1 + 100µM, SK-Hep-1: 6 + 100µM) for 48 h. Flow cytometric analysis was then performed to monitor apoptosis. (F) Huh7 and SK-Hep-1 cells were treated with different drugs. Western blot was performed to monitor the expression of cleaved caspase-3 and cleaved PARP1. (G) Huh7 and SK-Hep-1 cells were treated with different drugs. Western blot was performed to monitor the expression of EGFR, pEGFR, AKT, pAKT, STAT3, pSTAT3. Statistical significance was determined by two-tailed Student’s t-test (two groups) or one-way ANOVA followed by Tukey’s post hoc test (multiple groups).* p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Sop (HY-N0423) and lenvatinib (HY-10981) were provided by MedChemExpress (MCE, China).

Techniques: CCK-8 Assay, Software, Colony Assay, Western Blot, Expressing, Two Tailed Test

Journal: Scientific Reports

Article Title: Sophoricoside from sophora japonica L. is efficacious as monotherapy or in combination with lenvatinib in hepatocellular carcinoma via targeting EGFR

doi: 10.1038/s41598-025-33330-x

Figure Lengend Snippet: Lenvatinib combined with Sop better suppressed HCC growth in vivo. Photographs showing tumors in Huh7 xenografts under different treatments. Tumor growth curves under different treatments, Observed TGI/Expected TGI ratio = 70%/58% = 1.21. Final tumor weight after different treatments. (D and F)Western blot was performed to analyze the expression of the indicated proteins from the xenograft tumors. (E and G)Protein quantification for each group. Statistical significance was determined by two-tailed Student’s t-test (two groups) or one-way ANOVA followed by Tukey’s post hoc test (multiple groups). * p < 0.05, ** p < 0.01, *** p < 0.001, ns: no significance.

Article Snippet: Sop (HY-N0423) and lenvatinib (HY-10981) were provided by MedChemExpress (MCE, China).

Techniques: In Vivo, Western Blot, Expressing, Two Tailed Test

Journal: Scientific Reports

Article Title: Sophoricoside from sophora japonica L. is efficacious as monotherapy or in combination with lenvatinib in hepatocellular carcinoma via targeting EGFR

doi: 10.1038/s41598-025-33330-x

Figure Lengend Snippet: Schematic model drawn by Figdraw illustrating the inhibitory mechanism of sophoricoside and with lenvatinib against HCC.

Article Snippet: Sop (HY-N0423) and lenvatinib (HY-10981) were provided by MedChemExpress (MCE, China).

Techniques: