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glycotechnica lectin microarrays (GlycoTechnica Ltd)

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GlycoTechnica Ltd glycotechnica lectin microarrays
Glycotechnica Lectin Microarrays, supplied by GlycoTechnica Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lectin+microarray/lectin+microarray+chip+lecchiptm/pm40644615-16-10-10
Average 90 stars, based on 1 article reviews

glycotechnica lectin microarrays - by Bioz Stars, 2026-10

90/100 stars

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Concentration Assay:

Article Title: Novel GNE Gene Variants Associated with Severe Congenital Thrombocytopenia and Platelet Sialylation Defect
Article Snippet: Samples were incubated for 1 hour at room temperature in the dark and with excess Cy3 removed using a Zeba Spin Desalting Column (Thermo Fisher Scientific, Boston, Massachusetts, United States). .. Optimized concentration (0.5 μg) of glycoprotein was added to an individual lectin microarray with 45 printed lectins ( Supplementary Table S1 , available in the online version) each in triplicate (GlycoTechnica, Yokohama, Japan). .. Fluorescent microarray images were acquired and analyzed using an evanescent-field fluorescence scanner using Signal Capture and GlycoStation Tools Pro (v1.5, GlycoTechnica, Yokohama, Japan).

Microarray:

Article Title: Glycosylation of recombinant adeno-associated virus serotype 6
Article Snippet: .. We thank Sayuki Iijima, Kaori Yamauchi, and Hiroko Shimazaki (GlycoTechnica Ltd., Yokohama, Japan) for support with the lectin microarray analyses and Tamaki Aoki, Mika Kishimoto, and Hiromi Ozaki (Jichi Medical University, Tochigi, Japan) for help with the measurement of in vivo transduction efficiency. .. The image of the mouse and syringe in graphical abstract was created with BioRender.

Article Title: Analysis of a lectin microarray identifies altered sialylation of mouse serum glycoproteins induced by whole-body radiation exposure
Article Snippet: .. Analysis of changes in the glycosylation pattern of serum glycoproteins ( n = 2) was performed by GlycoTechnica Ltd (Yokohama, Japan) using a lectin microarray as described in Hirabayashi et al. [ ]. .. For each experimental and control run, the serum protein concentration was determined (Micro BCA Protein Assay Reagent Kit, Thermo Scientific, #23235), and 1 μg total serum protein was labeled with Cy3 Mono-Reactive dye (#PA23011, GE Healthcare, Indianapolis, IN, USA) for 1 h in the dark.

In Vivo:

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Glycoproteomics:

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$High MBL pathway activity is enriched in PCa liver metastasis and GalNAc is a major glycosylation form on NEPC cell surface. a) The KEGG analysis showing that the “complement and coagulation cascades” is most upregulated signature in liver metastasis versus primary prostate tumors of rb1 Δ/Δ p53 Δ/Δ NEPC tumor‐bearing mice (n = 3, mice). b,c) GSEA plots reveal that “complement and coagulation cascade” (b) and “MBL pathway activity” are significantly elevated in liver metastatic lesions compared to metastasis in other anatomic sites based on analysis of the SU2C prostate cancer dataset. d) Kaplan–Meier survival analysis of the SU2C prostate cancer dataset reveal that PCa patients with high MBL activity (n = 39) exhibit significantly shorter survival compared to PCa patients with low MBL activity (n = 39). The log rank‐test was applied for statistics. e,f) GSEA plots showing that the “cell surface glycosylation” is significantly elevated in NEPC compared to PrAD based on analysis of Beltran and SU2C prostate cancer datasets. g) Lectin microarray results that the O‐GalNAc glycosylation, as revealed by the strongest signal of vicia villosa (VVA) lectin toward the membrane fraction extracted from murine rb1 Δ/Δ p53 Δ/Δ NEPC organoids.$

Journal: Advanced Science

Article Title: O‐GalNAc Glycosylation Activates MBL‐Mediated Complement and Coagulation Cascades to Drive Organotropic Metastasis

doi: 10.1002/advs.202504809

Figure Lengend Snippet: High MBL pathway activity is enriched in PCa liver metastasis and GalNAc is a major glycosylation form on NEPC cell surface. a) The KEGG analysis showing that the “complement and coagulation cascades” is most upregulated signature in liver metastasis versus primary prostate tumors of rb1 Δ/Δ p53 Δ/Δ NEPC tumor‐bearing mice (n = 3, mice). b,c) GSEA plots reveal that “complement and coagulation cascade” (b) and “MBL pathway activity” are significantly elevated in liver metastatic lesions compared to metastasis in other anatomic sites based on analysis of the SU2C prostate cancer dataset. d) Kaplan–Meier survival analysis of the SU2C prostate cancer dataset reveal that PCa patients with high MBL activity (n = 39) exhibit significantly shorter survival compared to PCa patients with low MBL activity (n = 39). The log rank‐test was applied for statistics. e,f) GSEA plots showing that the “cell surface glycosylation” is significantly elevated in NEPC compared to PrAD based on analysis of Beltran and SU2C prostate cancer datasets. g) Lectin microarray results that the O‐GalNAc glycosylation, as revealed by the strongest signal of vicia villosa (VVA) lectin toward the membrane fraction extracted from murine rb1 Δ/Δ p53 Δ/Δ NEPC organoids.

Article Snippet: Utilizing a commercial lectin microarray kit (RayBiotech, GA‐Lectin‐70‐14) including a total of 70 lectin proteins, an analysis on glycan expression profiles was conducted using murine rb1 Δ/Δ p53 Δ/Δ NEPC cells, based on the manufacturer's guidelines.

Techniques: Activity Assay, Glycoproteomics, Coagulation, Microarray, Membrane

Journal: iScience

Article Title: Elevated high-mannose N-glycans hamper endometrial decidualization

doi: 10.1016/j.isci.2023.108170

Figure Lengend Snippet:

Article Snippet: Lectin microarray of decidual tissues from early pregnant women (n = 3) and miscarriage patients (n = 3) was performed using Lectin microarray (GA-Lectin-70, Raybiotech, USA).

Techniques: Microarray, Bicinchoninic Acid Protein Assay, Recombinant, Plasmid Preparation, Software