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lectin microarray kit  (RayBiotech inc)


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    Structured Review

    RayBiotech inc lectin microarray kit
    High MBL pathway activity is enriched in PCa liver metastasis and GalNAc is a major glycosylation form on NEPC cell surface. a) The KEGG analysis showing that the “complement and coagulation cascades” is most upregulated signature in liver metastasis versus primary prostate tumors of rb1 Δ/Δ p53 Δ/Δ NEPC tumor‐bearing mice (n = 3, mice). b,c) GSEA plots reveal that “complement and coagulation cascade” (b) and “MBL pathway activity” are significantly elevated in liver metastatic lesions compared to metastasis in other anatomic sites based on analysis of the SU2C prostate cancer dataset. d) Kaplan–Meier survival analysis of the SU2C prostate cancer dataset reveal that PCa patients with high MBL activity (n = 39) exhibit significantly shorter survival compared to PCa patients with low MBL activity (n = 39). The log rank‐test was applied for statistics. e,f) GSEA plots showing that the “cell surface glycosylation” is significantly elevated in NEPC compared to PrAD based on analysis of Beltran and SU2C prostate cancer datasets. g) <t>Lectin</t> <t>microarray</t> results that the O‐GalNAc glycosylation, as revealed by the strongest signal of vicia villosa (VVA) lectin toward the membrane fraction extracted from murine rb1 Δ/Δ p53 Δ/Δ NEPC organoids.
    Lectin Microarray Kit, supplied by RayBiotech inc, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lectin+microarray/Lectin+Array+70/pmc12407318-239-3-6
    Average 93 stars, based on 11 article reviews
    lectin microarray kit - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "O‐GalNAc Glycosylation Activates MBL‐Mediated Complement and Coagulation Cascades to Drive Organotropic Metastasis"

    Article Title: O‐GalNAc Glycosylation Activates MBL‐Mediated Complement and Coagulation Cascades to Drive Organotropic Metastasis

    Journal: Advanced Science

    doi: 10.1002/advs.202504809

    High MBL pathway activity is enriched in PCa liver metastasis and GalNAc is a major glycosylation form on NEPC cell surface. a) The KEGG analysis showing that the “complement and coagulation cascades” is most upregulated signature in liver metastasis versus primary prostate tumors of rb1 Δ/Δ p53 Δ/Δ NEPC tumor‐bearing mice (n = 3, mice). b,c) GSEA plots reveal that “complement and coagulation cascade” (b) and “MBL pathway activity” are significantly elevated in liver metastatic lesions compared to metastasis in other anatomic sites based on analysis of the SU2C prostate cancer dataset. d) Kaplan–Meier survival analysis of the SU2C prostate cancer dataset reveal that PCa patients with high MBL activity (n = 39) exhibit significantly shorter survival compared to PCa patients with low MBL activity (n = 39). The log rank‐test was applied for statistics. e,f) GSEA plots showing that the “cell surface glycosylation” is significantly elevated in NEPC compared to PrAD based on analysis of Beltran and SU2C prostate cancer datasets. g) Lectin microarray results that the O‐GalNAc glycosylation, as revealed by the strongest signal of vicia villosa (VVA) lectin toward the membrane fraction extracted from murine rb1 Δ/Δ p53 Δ/Δ NEPC organoids.
    Figure Legend Snippet: High MBL pathway activity is enriched in PCa liver metastasis and GalNAc is a major glycosylation form on NEPC cell surface. a) The KEGG analysis showing that the “complement and coagulation cascades” is most upregulated signature in liver metastasis versus primary prostate tumors of rb1 Δ/Δ p53 Δ/Δ NEPC tumor‐bearing mice (n = 3, mice). b,c) GSEA plots reveal that “complement and coagulation cascade” (b) and “MBL pathway activity” are significantly elevated in liver metastatic lesions compared to metastasis in other anatomic sites based on analysis of the SU2C prostate cancer dataset. d) Kaplan–Meier survival analysis of the SU2C prostate cancer dataset reveal that PCa patients with high MBL activity (n = 39) exhibit significantly shorter survival compared to PCa patients with low MBL activity (n = 39). The log rank‐test was applied for statistics. e,f) GSEA plots showing that the “cell surface glycosylation” is significantly elevated in NEPC compared to PrAD based on analysis of Beltran and SU2C prostate cancer datasets. g) Lectin microarray results that the O‐GalNAc glycosylation, as revealed by the strongest signal of vicia villosa (VVA) lectin toward the membrane fraction extracted from murine rb1 Δ/Δ p53 Δ/Δ NEPC organoids.

    Techniques Used: Activity Assay, Glycoproteomics, Coagulation, Microarray, Membrane

    Related Articles

    Glycoproteomics:

    Article Title: Brucella-driven host N-glycome remodeling controls infection.
    Article Snippet: .. ; cellular proteins were then harvested and subjected to glycoprotein analysis using either a lectin array (Lectin Array 70, RayBiotech, USA) or LC-MS/MS-based glycan abundance and structure analysis. (A–C) HEK293T cells expressing GFP or Rhg1-GFP display differential N-glycan abundance (A) or glycan structure distribution (B), and statistical analysis of the indicated N-glycan structures (C) in cells at 24 h.p.t. (D–H) Representative diagrams of the indicated specific N-glycan structures of high-mannose (HMS, D), fucosylated structures (FS, E), sialylated structures (SS, F), fucosylated + sialylated structures (F&SS, G), and other glycan structures without sialic acid and fucose (OS, H) changed in cells expressing Rhg1-GFP (24 h.p.t). (legend continued on next page) Cell Host & Microbe 32, 588–605, April 10, 2024 595 of the host OST complex and the ER translocon may regulate the intracellular lifestyle of Brucella. ..

    Article Title: Effects of cryopreservation on the glycan profile of Symbiodiniaceae.
    Article Snippet: Symon Binay and HsingHui Li have made equal contributions.. Abbreviations: AAL, Aleuria aurantia; ACG, Agrocybe cylindracea galectin; ACL, Amaranthus caudatus; ATP, adenosine triphosphate; BanLec, Musa acuminata lectin; BC2LCN, Burkholderia cenocepacia lectin; CNL, Clitocybe nebularis lectin; Con A, concanavalin A; CPA, cryoprotective agent; DBA, Dolichos biflorus agglutinin; discoidin II, Dictyostelium discoideum lectin; DNA, deoxyribonucleic acid; F17AG, Escherichia coli lectin; FASW, filtered artificial seawater; FSW, filtered seawater; Gal1, galectin1; Gal1S, Human galectin1S lectin; Gal2, galectin2; Gal3, galectin3; GalGalNAc, GalactoseNAcetylgalactosamine; GalGlcNAc, GalactoseNAcetylglucosamine; GalNAc, NAcetylglactosamine; GlcNAc, NAcetylglucosamine; GRFT, Griffithia sp. lectin; HHA, Hippeastrum hybrid; LacNAc, Nacetyllactosamine; LSLN, Laetiporus sulphureus lectin; MFI, mean fluorescence intensity; NPA, Narcissus pseudonarcissus; Orysata, Oryza sativa lectin; PBS, phosphatebuffered saline; PHAP, Phaseolus vulgaris agglutinin; PNA, peanut agglutinin; PSL1a, Polyporus squamosus lectin 1a; PTL, Psophocarpus tetragonolobus lectin; RSFuc, Ralstonia solanacearum lectin; VVA, Vicia villosa agglutinin.. Department of Marine Biotechnology and Resources, National Sun YatSen University, Kaohsiung, Taiwan National Museum of Marine Biology & Aquarium, Pingtung, Taiwan Graduate Institute of Marine Biology, National Dong Hwa University, Pingtung, Taiwan Department of PostModern Agriculture, Mingdao University, Chang Hua, Taiwan VIP CORALSLobo and College of Arts and Sciences, Batangas State University, The National Engineering University, Batangas, Philippines

    Article Title: O‐GalNAc Glycosylation Activates MBL‐Mediated Complement and Coagulation Cascades to Drive Organotropic Metastasis
    Article Snippet: .. Utilizing a commercial lectin microarray kit (RayBiotech, GA‐Lectin‐70‐14) including a total of 70 lectin proteins, an analysis on glycan expression profiles was conducted using murine rb1 Δ/Δ p53 Δ/Δ NEPC cells, based on the manufacturer's guidelines. ..

    Expressing:

    Article Title: Brucella-driven host N-glycome remodeling controls infection.
    Article Snippet: .. ; cellular proteins were then harvested and subjected to glycoprotein analysis using either a lectin array (Lectin Array 70, RayBiotech, USA) or LC-MS/MS-based glycan abundance and structure analysis. (A–C) HEK293T cells expressing GFP or Rhg1-GFP display differential N-glycan abundance (A) or glycan structure distribution (B), and statistical analysis of the indicated N-glycan structures (C) in cells at 24 h.p.t. (D–H) Representative diagrams of the indicated specific N-glycan structures of high-mannose (HMS, D), fucosylated structures (FS, E), sialylated structures (SS, F), fucosylated + sialylated structures (F&SS, G), and other glycan structures without sialic acid and fucose (OS, H) changed in cells expressing Rhg1-GFP (24 h.p.t). (legend continued on next page) Cell Host & Microbe 32, 588–605, April 10, 2024 595 of the host OST complex and the ER translocon may regulate the intracellular lifestyle of Brucella. ..

    Article Title: O‐GalNAc Glycosylation Activates MBL‐Mediated Complement and Coagulation Cascades to Drive Organotropic Metastasis
    Article Snippet: .. Utilizing a commercial lectin microarray kit (RayBiotech, GA‐Lectin‐70‐14) including a total of 70 lectin proteins, an analysis on glycan expression profiles was conducted using murine rb1 Δ/Δ p53 Δ/Δ NEPC cells, based on the manufacturer's guidelines. ..

    Fluorescence:

    Article Title: Effects of chilling and cryoprotectants on glycans in shrimp embryos.
    Article Snippet: Glycans are carbohydrates present in every organism that bind to specific molecules such as lectins, a diverse group of proteins.. Glycans are vital to cell proliferation and protein trafficking.. In addition, embryogenesis is a critical phase in the development of marine organisms.

    Microarray:

    Article Title: Effects of chilling and cryoprotectants on glycans in shrimp embryos.
    Article Snippet: Glycans are carbohydrates present in every organism that bind to specific molecules such as lectins, a diverse group of proteins.. Glycans are vital to cell proliferation and protein trafficking.. In addition, embryogenesis is a critical phase in the development of marine organisms.

    Article Title: O‐GalNAc Glycosylation Activates MBL‐Mediated Complement and Coagulation Cascades to Drive Organotropic Metastasis
    Article Snippet: .. Utilizing a commercial lectin microarray kit (RayBiotech, GA‐Lectin‐70‐14) including a total of 70 lectin proteins, an analysis on glycan expression profiles was conducted using murine rb1 Δ/Δ p53 Δ/Δ NEPC cells, based on the manufacturer's guidelines. ..

    Article Title: Elevated high-mannose N-glycans hamper endometrial decidualization
    Article Snippet: .. Lectin Microarray , Raybiotech , GA-Lectin 70. .. BCA Protein Assay Kit , Beyotime , P0012S.



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    Image Search Results


    High MBL pathway activity is enriched in PCa liver metastasis and GalNAc is a major glycosylation form on NEPC cell surface. a) The KEGG analysis showing that the “complement and coagulation cascades” is most upregulated signature in liver metastasis versus primary prostate tumors of rb1 Δ/Δ p53 Δ/Δ NEPC tumor‐bearing mice (n = 3, mice). b,c) GSEA plots reveal that “complement and coagulation cascade” (b) and “MBL pathway activity” are significantly elevated in liver metastatic lesions compared to metastasis in other anatomic sites based on analysis of the SU2C prostate cancer dataset. d) Kaplan–Meier survival analysis of the SU2C prostate cancer dataset reveal that PCa patients with high MBL activity (n = 39) exhibit significantly shorter survival compared to PCa patients with low MBL activity (n = 39). The log rank‐test was applied for statistics. e,f) GSEA plots showing that the “cell surface glycosylation” is significantly elevated in NEPC compared to PrAD based on analysis of Beltran and SU2C prostate cancer datasets. g) Lectin microarray results that the O‐GalNAc glycosylation, as revealed by the strongest signal of vicia villosa (VVA) lectin toward the membrane fraction extracted from murine rb1 Δ/Δ p53 Δ/Δ NEPC organoids.

    Journal: Advanced Science

    Article Title: O‐GalNAc Glycosylation Activates MBL‐Mediated Complement and Coagulation Cascades to Drive Organotropic Metastasis

    doi: 10.1002/advs.202504809

    Figure Lengend Snippet: High MBL pathway activity is enriched in PCa liver metastasis and GalNAc is a major glycosylation form on NEPC cell surface. a) The KEGG analysis showing that the “complement and coagulation cascades” is most upregulated signature in liver metastasis versus primary prostate tumors of rb1 Δ/Δ p53 Δ/Δ NEPC tumor‐bearing mice (n = 3, mice). b,c) GSEA plots reveal that “complement and coagulation cascade” (b) and “MBL pathway activity” are significantly elevated in liver metastatic lesions compared to metastasis in other anatomic sites based on analysis of the SU2C prostate cancer dataset. d) Kaplan–Meier survival analysis of the SU2C prostate cancer dataset reveal that PCa patients with high MBL activity (n = 39) exhibit significantly shorter survival compared to PCa patients with low MBL activity (n = 39). The log rank‐test was applied for statistics. e,f) GSEA plots showing that the “cell surface glycosylation” is significantly elevated in NEPC compared to PrAD based on analysis of Beltran and SU2C prostate cancer datasets. g) Lectin microarray results that the O‐GalNAc glycosylation, as revealed by the strongest signal of vicia villosa (VVA) lectin toward the membrane fraction extracted from murine rb1 Δ/Δ p53 Δ/Δ NEPC organoids.

    Article Snippet: Utilizing a commercial lectin microarray kit (RayBiotech, GA‐Lectin‐70‐14) including a total of 70 lectin proteins, an analysis on glycan expression profiles was conducted using murine rb1 Δ/Δ p53 Δ/Δ NEPC cells, based on the manufacturer's guidelines.

    Techniques: Activity Assay, Glycoproteomics, Coagulation, Microarray, Membrane

    Journal: iScience

    Article Title: Elevated high-mannose N-glycans hamper endometrial decidualization

    doi: 10.1016/j.isci.2023.108170

    Figure Lengend Snippet:

    Article Snippet: Lectin microarray of decidual tissues from early pregnant women (n = 3) and miscarriage patients (n = 3) was performed using Lectin microarray (GA-Lectin-70, Raybiotech, USA).

    Techniques: Microarray, Bicinchoninic Acid Protein Assay, Recombinant, Plasmid Preparation, Software