Journal: PLoS ONE
Article Title: Comparative Transcriptomic Analysis of Developing Cotton Cotyledons and Embryo Axis
doi: 10.1371/journal.pone.0071756
Figure Lengend Snippet: 9 unigenes were selected for qRT-PCR to validate the expression patterns in different samples. The grey bar represent the relative intensity of real time qRT-PCR from independent biological replicates (using the left Y axis), the red squares represent the expression level (RPKM) of the transcript (using the right Y axis) and are connected by the red trend lines. The unigenes are: A, unigene42855; B, unigene70917; C, unigene36175; D, unigene 72799; E, unigene4514; F, unigene37407; G, unigene25168; H, unigene29210 and I, unigene19199.
Article Snippet: For example, unigene70917, annotated as late embryogenesis abundant 3 (LEA3) protein , unigene36175 annotated as ATS/KAN4 transcription factor ( ) and unigene 72799, annotated as cyclopropane fatty acid synthase ( ) appeared to express only in the embryo axis, with little if any, expression in cotyledons as indicated by the transcriptome analysis.
Techniques: Quantitative RT-PCR, Expressing