rabbit anti ngal  (Proteintech)

Journal: bioRxiv

Article Title: The RNA-binding protein Imp1 promotes a Spdef transcriptional program and mucus fucosylation during necrotizing enterocolitis

doi: 10.64898/2026.01.30.702645

Figure Lengend Snippet: A) Gross images of intestine from dam-fed and NEC mice. Putative pneumatosis is indicated by arrows. B) Cytokine gene expression for Il1b and Lcn2 was assessed in whole-thickness intestine using qRT-PCR in Imp1 IEC-OE versus wild-type dam-fed and NEC mice. Each data point represents one animal, n=30-31 mice per group. The data are not normally distributed and were therefore analyzed using the Mann-Whitney test. Error bars represent the SEM, * P <0.05. C) NEC mice exhibit epithelial vacuolization and damage assessed by hemotoxylin & eosin (H&E) staining and scored D) by a pathologist blinded to animal genotype and treatment. Scores were assigned as follows: 0 = no abnormality; 1 = mild vacuolization; 2 = full-thickness vacuolization; 3 = vacuolization with basal detachment of epithelium. Each dot represents one animal, n = 12-16 mice per group. Data were analyzed by a 2-way ANOVA with Tukey’s test for multiple comparisons. Error bars represent the SEM and * P <0.05 was considered statistically significant.

Article Snippet: Mouse : insulin-like growth factor 2 mRNA binding protein (Igf2bp1, Mm00501602_m1), interleukin 1 beta (Il1B, Mm00434228_m1), lipocalin 2 (LCN2, Mm01324470_m1), hypoxanthine guanine phosphoribosyl transferase (Hprt, Mm03024075_m1).

Techniques: Gene Expression, Quantitative RT-PCR, MANN-WHITNEY, Staining

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: An Epigenetic Basis for Sustained Inflammatory Epithelial Progenitor Cell States in Crohn’s Disease

doi: 10.1016/j.jcmgh.2025.101665

Figure Lengend Snippet: Cells expressing ISP markers in colonic mucosa in CD tissue. ( A–B ) Representative single channel ( A ) and merged ( B ) images of immunostaining for LCN2, CD74, HLA-DR in biopsies from study subjects, quantified in ( C ) (N = 2 subjects per group, n = 11–16 crypts analyzed per biopsy, average per subject plotted on graph). Asterisk denotes an ISP. ( D ) Correlation analysis comparing abundance of ISPs in tissue (N = 4) as quantified at transcript level (scRNA-seq) to quantification at protein level (immunostaining) in matched subjects (N = 4). P values: ( C ) Welch’s t -test (unpaired, 2-tailed); ( D ) nonparametric Spearman correlation (2-tailed).

Article Snippet: Real-time quantitative polymerase chain reaction (RT-qPCR) was performed with TaqMan probes (TBP1: Hs00427621_m1; LCN2: Hs01008571_m1; HLA-DRA: Hs00219575_m1; HLA-DPA1: Hs00410276_m1; CD74: Hs00269961_m1) and TaqMan Fast Universal PCR Master Mix 2X (Catalog #4352042).

Techniques: Expressing, Immunostaining

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: An Epigenetic Basis for Sustained Inflammatory Epithelial Progenitor Cell States in Crohn’s Disease

doi: 10.1016/j.jcmgh.2025.101665

Figure Lengend Snippet: ISP state can be experimentally induced in patient-derived colonoids. ( A ) Schematic of experimental approach and data presentation. ( B ) Expression of ISP marker genes in colonoids from CD or control subjects (N = 3 each, Passage = 4–6). ( C ) Correlation analysis of HLA-DRA gene expression in the study subjects’ biopsies (scRNA-seq data, epithelial cluster) vs HLA-DR protein expression in colonoids (N = 4 controls and 5 Crohn's) from corresponding subjects (flow cytometry), comparing colonoids grown either in stem cell-enriching expansion medium ( black squares ) or differentiation medium ( open circles ). ( D ) Representative images of immunostaining for LCN2, CD74, HLA-DR in colonoids from study subjects. Asterisk denotes an ISP. Quantified in ( E ): N = 2 lines (n = 12 high power fields) per group, Passage = 7–8. ( F ) Quantification (flow cytometry) of %HLA-DR+ cells in colonoids treated with the inflammatory cocktail (20 ng/mL IL-1beta, 100 ng/mL TNF-alpha, 1 μg/mL flagellin, and 10 ng/mL IL-6) for 24 hours (N = 7 or 8 lines from control subjects or patients with CD, respectively, Passage = 6–8). P values: ( B ) Mann-Whitney test (unpaired, 2-tailed), ( E–F ) ordinary 2-way analysis of variance with Sidak’s multiple comparisons.

Article Snippet: Real-time quantitative polymerase chain reaction (RT-qPCR) was performed with TaqMan probes (TBP1: Hs00427621_m1; LCN2: Hs01008571_m1; HLA-DRA: Hs00219575_m1; HLA-DPA1: Hs00410276_m1; CD74: Hs00269961_m1) and TaqMan Fast Universal PCR Master Mix 2X (Catalog #4352042).

Techniques: Derivative Assay, Expressing, Marker, Control, Gene Expression, Flow Cytometry, Immunostaining, MANN-WHITNEY

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: An Epigenetic Basis for Sustained Inflammatory Epithelial Progenitor Cell States in Crohn’s Disease

doi: 10.1016/j.jcmgh.2025.101665

Figure Lengend Snippet: HLA-DR+HLA-DP+ cells from colonoids subjected to inflammatory cocktail express ISP marker genes. ( A ) Schematic: Colonoids from from control subjects or patients with CD were treated with the inflammatory cocktail for 24 hours and subjected to flow cytometry sorting of HLA-DR+ and HLA-DR+HLA-DP+ populations, as well as total live cells to use as control. ( B ) Quantification (flow cytometry) of %HLA-DR+HLA-DP+ cells in colonoids treated with the inflammatory cocktail for 24 hours. One line each from control subject or patient with CD, N = 3 independent experiments repeated in different passages (Passage = 4–6). ( C ) Expression of ISP marker genes HLA-DPA1, HLA-DRA, CD74, and LCN2, in cell populations sorted as described in ( A ) from control or CD colonoids. One line each from control subject or patient with CD, N = 3 independent experiments repeated in different passages (Passage = 4–6), representative data from 1 experiment are shown. P values: ( B ) ordinary 2-way analysis of variance with Sidak’s multiple comparisons.

Article Snippet: Real-time quantitative polymerase chain reaction (RT-qPCR) was performed with TaqMan probes (TBP1: Hs00427621_m1; LCN2: Hs01008571_m1; HLA-DRA: Hs00219575_m1; HLA-DPA1: Hs00410276_m1; CD74: Hs00269961_m1) and TaqMan Fast Universal PCR Master Mix 2X (Catalog #4352042).

Techniques: Marker, Control, Flow Cytometry, Expressing