Journal: Frontiers in Neuroscience
Article Title: In silico Screening and Behavioral Validation of a Novel Peptide, LCGA-17, With Anxiolytic-Like Properties
doi: 10.3389/fnins.2021.705590
Figure Lengend Snippet: Behavioral analyses of the anxiolytic-like properties of LCGA tetra-peptides in Danio rerio . (A,B) In the NTT, LCGA-17 (10 mg/kg) increased the time the fish spent in the upper half of the tank, similar to fluvoxamine (Flv, 5 mg/kg), indicating an anxiolytic-like response (A) . By contrast, diazepam (Diaz, 5 mg/kg) reduced the time spent in the upper half of the tank (A) . None of the treatments affected the distance traveled (B) . (C) In the LDB, LCGA-17 increased the time spent in the lit compartment, closely mimicking the anxiolytic-like effect of diazepam. (D) In the SPT, both LGCA-17 and LCGA-26 increased the time spent out of the shoaling zone, again mimicking an anxiolytic-like effect of diazepam. Neither fluvoxamine (5 mg/kg) nor LCGA-59 and LCGA-83 (10 mg/kg) affected the behavior in the LDB and SPT (B,C) . Data are presented as % of vehicle control. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p ≤ 0.001, n = 18–30, Mann–Whitney, with corrections for multiple comparisons using the FDR method, at a q threshold of 0.05.
Article Snippet: LCGA peptides (supplied by Lactocore, Inc., 98% purity) were administered at a dose of 1 and 10 mg/kg (i.p., prepared in saline on the day of the experiment).
Techniques: Control, MANN-WHITNEY
