lamp1 (Proteintech)
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Proteintech
lamp1
Lamp1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 258 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lamp1/LAMP1+Antibody/pmc12933830-129-35-36
Average 96 stars, based on 258 article reviews
Lamp1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 258 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lamp1/LAMP1+Antibody/pmc12933830-129-35-36
Average 96 stars, based on 258 article reviews
lamp1 - by Bioz Stars,
2026-09
96/100 stars
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Infection:Article Title: Parvimonas micra exacerbates periodontitis by infiltrating host cells through TmpC and circumventing lysosomal elimination via AppA. Article Snippet: F-actin and the nuclei of PDLSCs were stained with phalloidin (Abcam, ab176757) and DAPI, respectively. .. To visualise the subcellular localisation of P. micra during infection, P. micra and PDLSCs were stained with CFSE, EEA1 (Proteintech, 68065-1-Ig), Rab7 (Zenbio, R25524) and Staining:Article Title: Parvimonas micra exacerbates periodontitis by infiltrating host cells through TmpC and circumventing lysosomal elimination via AppA. Article Snippet: F-actin and the nuclei of PDLSCs were stained with phalloidin (Abcam, ab176757) and DAPI, respectively. .. To visualise the subcellular localisation of P. micra during infection, P. micra and PDLSCs were stained with CFSE, EEA1 (Proteintech, 68065-1-Ig), Rab7 (Zenbio, R25524) and Incubation:Article Title: β - sitosterol promotes the SUMOylation of DRP1 in alveolar macrophages and alleviates sepsis-associated acute lung injury. Article Snippet: Acute lung injury (ALI) represents the most frequent complication of sepsis; however, effective drug-based interventions are still unavailable. β-sitosterol (BS) has demonstrated anti-inflammatory effects and protective properties on alveolar epithelial barriers.. This study investigated the mechanism by which BS targets alveolar macrophages to attenuate sepsis-associated acute lung injury (SALI) via in vivo and in vitro experiments.. Sepsis was induced in mice through cecal ligation and puncture (CLP), and BS was administered orally. Article Title: Baicalin chemosensitivity enhancement of cisplatin in bladder cancer via autophagy flux inhibition Article Snippet: EDTA Antigen Retrieval Solution (Solarbio, China, C1034) was used for antigen retrieval. .. Then the sections were incubated with 0.3% H 2 O 2 for 10 min at room temperature, washed with PBS and blocked in 5% BSA at 37 °C for 30 min, followed by incubated with primary antibodies: Article Title: A foam cell-targeted lipophagy restoration strategy stabilizes vulnerable atherosclerotic plaques Article Snippet: .. To block nonspecific binding, membranes were incubated with 5% skim milk for 1 h. Thereafter, membranes were incubated overnight at 4 °C with primary antibodies against ABCA1, ABCG1, ACOX1, CPT1A, LC3 (ab192890, 1:2000, abcam), Article Title: ADGRG1-targeted hypoxia preconditioned extracellular vesicles ameliorate intervertebral disc degeneration by delivering taurine to disrupt the oxidative stress feedback loop-driven ferroptosis in nucleus pulposus cells Article Snippet: .. After permeabilization and blocking with 10% goat serum containing 0.2% Triton X-100, the sections were incubated with primary antibodies against ADGRG1 (1:50; sc-390192, Santa Cruz Biotechnology), TOM20(11802-1-AP, Proteintech), 4-HNE(68538-1-Ig, Proteintech), 4-HNE(HY-P81208, MCE), Ferritin (Rockland 200-401-090-0100), LAMP1( Article Title: Lipotoxic hepatocyte-derived UBQLN1 -enriched small extracellular vesicles activate hepatic stellate cells to promote hepatic fibrosis. Article Snippet: Metabolic dysfunction-associated steatohepatitis (MASH) serves as a primary contributor to liver fibrosis, cirrhosis, and hepatocellular carcinoma, yet specific diagnostic markers and therapeutic targets remain unavailable.. This study elucidates the molecular mechanism by which UBQLN1 (ubiquilin 1) promotes MASH-related liver fibrosis by regulating small extracellular vesicles (sEVs) secretion and the functionality of the lysosome-mitochondria axis, as well as its clinical significance.. Analysis of a multicenter cohort (n = 150) demonstrated significantly elevated UBQLN1 levels in both serum and serum-derived sEV from MASH patients, exhibiting diagnostic accuracies of 0.89 and 0.95, respectively. Article Title: A foam cell-targeted lipophagy restoration strategy stabilizes vulnerable atherosclerotic plaques. Article Snippet: .. To block nonspecific binding, membranes were incubated with 5% skim milk for 1 h. Thereafter, membranes were incubated overnight at 4 ◦C with primary antibodies against ABCA1, ABCG1, ACOX1, CPT1A, LC3 (ab192890, 1:2000, abcam), Blocking Assay:Article Title: A foam cell-targeted lipophagy restoration strategy stabilizes vulnerable atherosclerotic plaques Article Snippet: .. To block nonspecific binding, membranes were incubated with 5% skim milk for 1 h. Thereafter, membranes were incubated overnight at 4 °C with primary antibodies against ABCA1, ABCG1, ACOX1, CPT1A, LC3 (ab192890, 1:2000, abcam), Article Title: ADGRG1-targeted hypoxia preconditioned extracellular vesicles ameliorate intervertebral disc degeneration by delivering taurine to disrupt the oxidative stress feedback loop-driven ferroptosis in nucleus pulposus cells Article Snippet: .. After permeabilization and blocking with 10% goat serum containing 0.2% Triton X-100, the sections were incubated with primary antibodies against ADGRG1 (1:50; sc-390192, Santa Cruz Biotechnology), TOM20(11802-1-AP, Proteintech), 4-HNE(68538-1-Ig, Proteintech), 4-HNE(HY-P81208, MCE), Ferritin (Rockland 200-401-090-0100), LAMP1( Article Title: A foam cell-targeted lipophagy restoration strategy stabilizes vulnerable atherosclerotic plaques. Article Snippet: .. To block nonspecific binding, membranes were incubated with 5% skim milk for 1 h. Thereafter, membranes were incubated overnight at 4 ◦C with primary antibodies against ABCA1, ABCG1, ACOX1, CPT1A, LC3 (ab192890, 1:2000, abcam), Binding Assay:Article Title: A foam cell-targeted lipophagy restoration strategy stabilizes vulnerable atherosclerotic plaques Article Snippet: .. To block nonspecific binding, membranes were incubated with 5% skim milk for 1 h. Thereafter, membranes were incubated overnight at 4 °C with primary antibodies against ABCA1, ABCG1, ACOX1, CPT1A, LC3 (ab192890, 1:2000, abcam), Article Title: Wogonin derivative V8 enhances bortezomib efficacy in gastric carcinoma by disrupting lysosome-mediated drug resistance Article Snippet: Cell counting kit-8 (CCK-8) solution was obtained from Vazyme (A311-01/02, Nanjing, China). .. Primary antibodies against ATF4 , Bax , immunoglobulin heavy chain binding protein (Bip), Bcl-xL , β-actin, caspase-3, cleaved-caspase-3, caspase-9, caspase-12, cleaved-caspase-12, phosphorylated eukaryotic translation initiation factor 2 alpha (p-eIF2α), p62, TFEB, microtubule-associated protein 1A/1B-light chain 3 (LC3), Ki67 and horseradish-peroxidase-conjugated goat anti-mouse/rabbit secondary antibodies were obtained from Abclonal Technology (Wuhan, Hubei Province, China); primary antibodies against caseinolytic mitochondrial matrix peptidase proteolytic subunit (Clpp), Article Title: A foam cell-targeted lipophagy restoration strategy stabilizes vulnerable atherosclerotic plaques. Article Snippet: .. To block nonspecific binding, membranes were incubated with 5% skim milk for 1 h. Thereafter, membranes were incubated overnight at 4 ◦C with primary antibodies against ABCA1, ABCG1, ACOX1, CPT1A, LC3 (ab192890, 1:2000, abcam), Membrane:Article Title: Lipotoxic hepatocyte-derived UBQLN1 -enriched small extracellular vesicles activate hepatic stellate cells to promote hepatic fibrosis. Article Snippet: Metabolic dysfunction-associated steatohepatitis (MASH) serves as a primary contributor to liver fibrosis, cirrhosis, and hepatocellular carcinoma, yet specific diagnostic markers and therapeutic targets remain unavailable.. This study elucidates the molecular mechanism by which UBQLN1 (ubiquilin 1) promotes MASH-related liver fibrosis by regulating small extracellular vesicles (sEVs) secretion and the functionality of the lysosome-mitochondria axis, as well as its clinical significance.. Analysis of a multicenter cohort (n = 150) demonstrated significantly elevated UBQLN1 levels in both serum and serum-derived sEV from MASH patients, exhibiting diagnostic accuracies of 0.89 and 0.95, respectively. Ubiquitin Proteomics:Article Title: Wogonin derivative V8 enhances bortezomib efficacy in gastric carcinoma by disrupting lysosome-mediated drug resistance Article Snippet: Cell counting kit-8 (CCK-8) solution was obtained from Vazyme (A311-01/02, Nanjing, China). .. Primary antibodies against ATF4 , Bax , immunoglobulin heavy chain binding protein (Bip), Bcl-xL , β-actin, caspase-3, cleaved-caspase-3, caspase-9, caspase-12, cleaved-caspase-12, phosphorylated eukaryotic translation initiation factor 2 alpha (p-eIF2α), p62, TFEB, microtubule-associated protein 1A/1B-light chain 3 (LC3), Ki67 and horseradish-peroxidase-conjugated goat anti-mouse/rabbit secondary antibodies were obtained from Abclonal Technology (Wuhan, Hubei Province, China); primary antibodies against caseinolytic mitochondrial matrix peptidase proteolytic subunit (Clpp), |
