lamp  (StressMarq)

Journal: bioRxiv

Article Title: Functional border-associated macrophages limit Alzheimer’s Disease progression

doi: 10.64898/2026.01.31.703045

Figure Lengend Snippet: A , Representative micrographs of X-34 amyloid staining in cortex and hippocampus in 3-5 month-old Cre + 5xFAD (BAM2 depleted) and Cre - (BAM2 sufficient) 5xFAD littermates (top) with regions of interest (ROIs, numbered boxes below) depicting pial and penetrating CD31 + blood vessels in cortex and the hippocampal artery in hippocampus. B , Quantification of X-34 cortical area fraction normalized to littermates at 3-5 mo (n= 11 Cre - , 12 Cre + mice) and 7-9 mo (n=7 Cre - , 6 Cre + mice). C , as with ( B ) but for hippocampus. D , Quantification of the percentage of pial and penetrating blood vessel area covered with CAA, presented as fold change normalized to littermates at 3-5 mo (n= 6 Cre - , 6 Cre + mice) and 7-9mo (n=7 Cre - , 6 Cre + mice) mice. E-H , ELISA based quantification of 3 mo whole brain ( E) soluble Aβ 1-40 (n= 6 Cre - , 7 Cre + mice), ( F) soluble Aβ 1-42 (n= 6 Cre - , 7 Cre + mice), ( G ) insoluble Aβ 1-40 (n= 5 Cre - , 4 Cre + mice), and ( H ) insoluble Aβ 1-42 (n= 6 Cre - , 6 Cre + mice). I , representative micrographs of LAMP1 staining in cortex and hippocampus in 3-5 mo Cre + and Cre - littermates, along with high magnification ROIs (numbered boxes below) in sections. Insets 1 and 2 show sections from Cre + mice depicting LAMP1 localization around X-34 + plaque (Inset 1) and vasculature (Inset 2, showing salient CAA). Inset 3 depicts CA3 granular cell layer in hippocampus from Cre + mice in which LAMP1 staining appears in gaps in NeuN+ neuron staining (red arrows). J , Quantification of cortical LAMP1 area fraction at 3-5mo (n=10 Cre - , 9 Cre + mice) and 7-9mo (n=7 Cre - , 7 Cre + mice); K , as with ( J ) but for hippocampal LAMP1 at 3-5mo (n=9 Cre - , n=12 Cre + mice), and 7-9mo (n= 6 Cre - , 5 Cre + mice). L-M , neuronal loss in Cre + mice. ( L ) representative micrograph depicting NeuN+ neuron staining in cortical layers from 9 mo Cre + and Cre - littermates; M , quantification of L5 neuron number/area normalized to littermates at 3-5 mo (n=6 Cre - , 6 Cre + mice) and 7-9 mo (n=8 Cre - , 6 Cre + ). N-O, behavioral tests. (N), schematic of contextual fear conditioning paradigm; ( O) , quantification of freezing proportion in first 5 minutes of memory retrieval (blue=male (M), red=female (F), n=8 WT M , 7WT F , 11 Cre + M , 10 Cre + F , 11 5xFAD M , 9 5xFAD F , 9 Cre + 5xFAD M , 10 Cre + 5xFAD F ). Main effect of sex (F(1,67)= 10.133, p = 0.002) and Cre status (F(1,67)= 10.012, p = 0.002). P , Schematic of Barnes maze test along with longitudinal design including trials, days, and age (months). Q-S Quantification, Cre x 5xFAD x Age interaction, and 5xFAD main effect respectively of ( Q ) average distance traveled (F(6,1855) = 2.978, p = 0.007) and (F(1,1855) = 6.200, p = 0.013); ( R ) committed (F(6,1856) = 3.267, p = 0.003) and (F(1,1856) = 12.550, p < .001); and ( S ) latency (s) to locate the escape hole for each day and age (F(6,1856) = 2.098, p = 0.051) and (F(1,1856) = 8.447, p = 0.004)). See Materials and Methods for n values). ( A, I ) White dotted line represents hippocampal area. ( B-D, J-K, M, O ) Two-way ANOVA with Sidak’s multiple comparisons test, ( E-H ) two-tailed unpaired t-test, ( Q-S ) Linear Mixed Effects Model with Sidak’s multiple comparisons test. Significant pairwise comparisons within time points indicated by shapes associated with respective genotypes in the panel legend. *P<.05,**P<.01, ***P<.001, ****p<.0001. ( A,I,L ) Scale bars=200µm large images, 50µm high mag ROIs. Fold change normalized to 1 (as average) includes individual values that are not 1; these derive from littermates with the same genotype and slightly different individual values.

Article Snippet: Sections were incubated ON at 4C with the following primary antibodies Goat anti-CD31 (1:150, AF3628, R& D systems), Rabbit anti-IBA1 (1:1000, 019-19741, Wako), Guinea Pig anti-NeuN (1:1000, 266 004, Synaptic systems), Rat anti-CD206 (1:500, MCA2235, Bio-Rad), Rat anti LAMP1 (1:250, 1D4B, DSHB), Rabbit anti-Laminin (1:300, Thermo Fisher, PA116730).

Techniques: Staining, Enzyme-linked Immunosorbent Assay, Two Tailed Test

Journal: bioRxiv

Article Title: Functional border-associated macrophages limit Alzheimer’s Disease progression

doi: 10.64898/2026.01.31.703045

Figure Lengend Snippet: A . Representative micrographs of X-34 amyloid staining in cortex and hippocampus in 7-9 month old Cre + (BAM2 depleted) and Cre - 5xFAD littermates with regions of interest (ROIs, green boxes) depicting CAA in pial and penetrating CD31 + blood vessels in cortex. B. As with A but for LAMP1 neurodystrophy marker. Scale bars=200µm large images, 50µm high mag ROIs.

Article Snippet: Sections were incubated ON at 4C with the following primary antibodies Goat anti-CD31 (1:150, AF3628, R& D systems), Rabbit anti-IBA1 (1:1000, 019-19741, Wako), Guinea Pig anti-NeuN (1:1000, 266 004, Synaptic systems), Rat anti-CD206 (1:500, MCA2235, Bio-Rad), Rat anti LAMP1 (1:250, 1D4B, DSHB), Rabbit anti-Laminin (1:300, Thermo Fisher, PA116730).

Techniques: Staining, Marker

Journal: The Journal of Cell Biology

Article Title: Rsp5/NEDD4 and ESCRT regulate TDP-43 toxicity and turnover via an endolysosomal clearance mechanism

doi: 10.1083/jcb.202212064

Figure Lengend Snippet: Giant Rab7 vesicular structures exhibit CD63 colocalization and CHQ-sensitive intraluminal CD63 bodies, suggesting an endolysosomal origin. (A) HEK293A cells expressing endogenous TDP-43 (WT) or TDP-43-GFP (pseudocolored white in merge) were immunostained for Rab7 (pseudocolored red) and CD63 (pseudocolored green). Percentages in zoom panels indicate Rab7 foci that overlap with CD63 foci. (B) TDP-43-GFP (pseudocolored white) expressing cells were immunostained for Rab7 (pseudocolored red in merge) and CD63 (pseudocolored green in merge) in the presence or absence of CHQ. Inclusion of multiple rows reflects diverse phenotypes observed. Green arrowheads indicate CD63 foci on the intraluminal Rab7 vesicular membrane (top row) or fully within Rab7 vesicles (second and fourth row). Red arrowheads indicate Rab7 puncta fully within Rab 7 vesicles (second row). White arrowheads indicate TDP-43 puncta within Rab7 vesicles (second and fourth row; bottom right in latter case), on the external Rab7 vesicular membrane (fifth row), or in distinct cytoplasmic puncta (fourth and sixth row). Scale bar, 10 µm. (C) Quantification of CD63 signal (average and by bins) within the lumen of Rab7 vesicles. *, P < 0.05 by Mann–Whitney U test. (D) Average Rab7 vesicle lumen area ± CHQ. ***, P < 0.001 by Mann–Whitney U test. (E) Percentage of TDP-43 localization phenotypes relative to Rab7 and CD63, binned by category. Error bars in all graphical panels represent standard error of the mean.

Article Snippet: Primary antibodies used for the immunofluorescence were Rab7 (ab137029; Abcam, dilution: 1:200), CD63 (H5C6; Developmental Studies Hybridoma Bank, dilution 1:100), Rab5 (ab109534; Abcam, dilution: 1:200), LC3B (3868S; Cell Signaling, dilution 1:500), LAMP1 (21997-1-AP; Proteintech, dilution 1:200), and TDP-43 (10782-2-AP; Proteintech; dilution 1:200).

Techniques: Expressing, Membrane, MANN-WHITNEY

Journal: International Journal of Biometeorology

Article Title: Effects of ultraviolet B radiation and cold storage on Ram sperm morphology and physiology

doi: 10.1007/s00484-025-03082-4

Figure Lengend Snippet: Mean values (± SEM) of total motility of ram spermatozoa immediately after UVB exposure (0 h – blue), and after 24 h (green) and 48 h (red) of cold storage at 5 °C. Sperm samples were evaluated following exposure to UVB radiation at different time intervals (0, 30, 60, 90, 120, and 150 s). Different lowercase letters above the bars indicate statistically significant differences ( p < 0.05) within each storage timepoint, i.e., differences between exposure times at 0 h, 24 h, and 48 h, respectively. Radiation 0 s = 0; 30 s = 2.199 mJ/cm2; 60 s = 4.398 mJ/cm2; 90 s = 6.597 mJ/cm2; 120 s = 8.796 mJ/cm2; 150 s = 10.995 mJ/cm2

Article Snippet: Radiation was delivered using a UVB lamp (VL-115 C, 30 W, peak emission at 312 nm; Vilber Lourmat, Marne Lavallée, France), positioned at a fixed distance from the samples.

Techniques:

Journal: International Journal of Biometeorology

Article Title: Effects of ultraviolet B radiation and cold storage on Ram sperm morphology and physiology

doi: 10.1007/s00484-025-03082-4

Figure Lengend Snippet: presents data on the structural integrity of ram spermatozoa after exposure to UVB radiation at different time intervals, assessed using epifluorescence microscopy. The figure includes results obtained immediately after exposure (0 h), as well as after 24 h and 48 h of cold storage at 5 °C. The analysis revealed no statistically significant DNA damage across treatments. However, UVB exposure led to a reduction in acrosomal and mitochondrial integrity, with the most pronounced damage observed after 150 s (10.995 mJ/cm²) of exposure. Moreover, the data indicate that the structural damage occurred at the moment of exposure and remained stable during the subsequent storage periods (24 h and 48 h), with no evidence of progression or recovery over time.10.995

Article Snippet: Radiation was delivered using a UVB lamp (VL-115 C, 30 W, peak emission at 312 nm; Vilber Lourmat, Marne Lavallée, France), positioned at a fixed distance from the samples.

Techniques: Epifluorescence Microscopy