Journal: Antioxidants
Article Title: Hyperoside Promotes Mitochondrial Autophagy Through the miR-361-5p/PI3K/Akt/mTOR Signaling Pathway, Thereby Improving UVB-Induced Photoaging
doi: 10.3390/antiox14121401
Figure Lengend Snippet: HY protects HaCaT from UVB-induced cellular senescence. ( A , B ) Representatives of SA-β-gal staining imaging and quantitative analysis. ( C ) The mRNA expressions of IL-1α , TNF-α , and VEGF were determined by RT-qPCR; ( D ) Representative images of γH2AX staining (Scale bars: 100 μm). The cells were immunostained with γH2AX (green) and Nuclei were stained with DAPI (blue). ( E ) Western blot detected aging markers p21 and p16. ( F , G ) quantitative analysis of p21 and p16. ( H ) Immunofluorescence assay for the expression of LaminB1 (Scale bars: 80 μm). The cells were immunostained with Tubulin (green), LaminB1 (red) and Nuclei were stained with DAPI (blue). ( I ) Expression of γH2AX fluorescence intensity. ( J ) Relative fluorescence intensity of LaminB1. Data are presented as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001. Ctrl (control group), UVB (the irradiated group), UVB 1H (1 μm of HY), UVB 5H (5 μm of HY).
Article Snippet: Antibodies were sourced as follows: p21 (10355-1-AP), Beclin1 (11306-1-AP), GAPDH (60004-1-Ig), LC3 (14600-1-AP), PI3K (20584-1-AP), p-Akt (66444-1-Ig), LaminB1 (12987-1-AP), TOM20 (11802-1-AP), and Tubulin (11224-1-AP) were purchased from Proteintech Group (Wuhan, China); γ-H2AX (M63324S) was from Abmart (Shanghai, China); Akt (YP-mAb-14652), mTOR (YP-mAb-14867), p-mTOR (YP-mAb-14329), and p-PI3K (YP-Ab-17956) were obtained from Upingbio (Hangzhou, China); p16 (CY5316), PINK1 (BY0130), Parkin (CY6641), and p62 (CY9081) were from Abways Technology (Shanghai, China).
Techniques: Staining, Imaging, Quantitative RT-PCR, Western Blot, Immunofluorescence, Expressing, Fluorescence, Control, Irradiation
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