lamb3 recombinant protein  (Proteintech)

Journal: bioRxiv

Article Title: Integrin beta 1 and mannose receptor 2 are involved in the antifungal activity of bronchial epithelial cells through Aspergillus fumigatus lectin FleA interactions

doi: 10.64898/2026.02.26.708144

Figure Lengend Snippet: ( A ) mRNA Laminin B3 and C2 expression after BECs transfection with siRNA-LAMB3 or siRNA-LAMC2. Data are expressed as percentage of control (siCtl) and presented as mean ± SD, n = 7 independent experiments. * p < 0.05, ** p < 0.01 (ANOVA test). ( B) Microscopic score of A. fumigatus filament formation after infection of BECs transfected with siRNA-LAMB3 and siRNA-LAMC2. BECs were transfected 48 h then infected for 15 h. Data are expressed as percentage of control (siCtl) and presented as mean ± SD, n = 10 independent experiments. *** p < 0.001 (ANOVA test). ( C ) Measure of galactomannan released in supernatant of infected BECs transfected with siRNA-LAMB3 or siRNA-LAMC2. Data are expressed as percentage of control (siCtl) and presented as mean ± SD, n= 8 independent experiments. * p < 0.05 (Student’s t-test). ( D ) Measure of galactomannan released in supernatant of BECs infected with A. fumigatus in presence of different concentrations of exogenous laminin-332. Data are expressed as percentage of control (0 µg/mL laminin-332) and presented as mean ± SEM, n = 3 independent experiments. * p < 0.05 (ANOVA test followed by Bonferroni’s multiple comparison test). (E) Conidia adhesion on BECs cells after 1 h of pre-incubation with laminin-332 (5 µg/mL) or FleA lectin (2 µM) and infection with 300 conidia/well during 2 h or 4 h. Control: Conidia adhesion to non-treated BECs. Data are expressed as % of control and presented as mean ± SD, n = 4 independent experiments. ** p < 0.01, *** p < 0.001 (ANOVA test followed by Bonferroni’s multiple comparison test).

Article Snippet: Reverse transcriptionwas was performed from 1μg RNA using a 2X RT Master Mix on a ProFlex thermocycler (Applied Biosystems, ThermoFischer), with the following program: 25°C for 10 min; 37°C for 2 h and 85°C for 5 min. Quantitative PCR was carried out using 2 μL of cDNA, 5 μL 2× TaqMan mix (Low ROX), 2.5 μL of RNase-free H 2 0 and 0.5 μL of the TaqMan probe: GAPDH (Hs02786624_g1); ITGB1 (Hs01127536_m1); MRC2 (Hs00195862_m1); LAMP1 (Hs00931461_m1); LAMB3 (Hs00165078_m1); LAMC2 (Hs01043717_m1) (Applied Biosystem).

Techniques: Expressing, Transfection, Control, Infection, Comparison, Incubation

Journal: PLOS One

Article Title: Identification and validation of palmitoylation-related signature genes based on machine learning for prostate cancer

doi: 10.1371/journal.pone.0338407

Figure Lengend Snippet: (A) Screening of prostate cancer cell lines. (B,C) Transfection efficiency and Gene expression level Verification, both mRNA and protein levels demonstrated statistically significant differences between the transfection cohorts and the control cohorts. (D,E) Both CCK-8 assays and EdU assays revealed that LAMB3 overexpression or knockdown significantly inhibited or enhanced the proliferative activity of LNCaP and PC3 cell lines. (F) The clone capacity of LNCaP and PC3 cells were inversely proportional to LAMB3 expression levels. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.

Article Snippet: Subsequently, the membranes were incubated overnight at 4°C with primary antibodies against LAMB3 Rabbit Polyclonal Antibody ( HA500480 ; 1:1000; HUABIO; China) and β-actin (R1207-1; 1:10,000; HUABIO; China).

Techniques: Transfection, Gene Expression, Control, CCK-8 Assay, Over Expression, Knockdown, Activity Assay, Expressing

Journal: PLOS One

Article Title: Identification and validation of palmitoylation-related signature genes based on machine learning for prostate cancer

doi: 10.1371/journal.pone.0338407

Figure Lengend Snippet: (A) In the LNCaP cell line, the migratory capacity of cells in the overexpression cohort was significantly reduced, while no significant differences were observed in the knockdown cohort. In contrast, both overexpression and knockdown cohorts of PC3 cells exhibited statistically significant differences in migratory capacity compared to the control cohort. (B) In Both PC3 and LNCaP cell lines, the expression level of LAMB3 was positively correlated with the level of apoptosis. (C) In the LNCaP cell line, there is no significant differences were observed in the migratory capacity between knockdown cohort and control cohort. Conversely, the migratory capacity of cells in the overexpression cohort was significantly superior to that of the control cohort. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.

Article Snippet: Subsequently, the membranes were incubated overnight at 4°C with primary antibodies against LAMB3 Rabbit Polyclonal Antibody ( HA500480 ; 1:1000; HUABIO; China) and β-actin (R1207-1; 1:10,000; HUABIO; China).

Techniques: Over Expression, Knockdown, Control, Expressing