Journal: Poultry Science
Article Title: miRNA profiling reveals that gga-let-7i/COL1A2 axis induces cell cycle arrest and triggers cellular senescence to accelerate ovarian aging in laying hens by suppressing the PI3K/AKT/MDM2 pathway
doi: 10.1016/j.psj.2026.106542
Figure Lengend Snippet: Prediction and validation of gga-let-7i target genes. (A) The target genes of gga-let-7i were predicted by Targetscan, Diana and miRDB data bases. (B) Top 25 of KEGG enrichment of predicted gga-let-7i target genes. (C and D) Expression of alternative gga-let-7i target gene in response to gga-let-7i overexpression and inhibition, n = 9. (E) Binding site between gga-let-7i and COL1A2 was predicted by RNAhybird. (F) Schematic diagram depicting the design of the gga-let-7i/COL1A2 dual-luciferase reporter assay. (G) Dual-luciferase reporter assay showed that gga-let-7i directly binds to 3′UTR region of COL1A2, n = 3. All data were derived from at least three independent replicates and are presented as the mean ± SEM. *, P < 0.05; ⁎⁎ , P < 0.01.
Article Snippet: Gene Ontology ( GO ) enrichment and Kyoto Encyclopedia of Genes and Genomes ( KEGG ) pathway analysis were conducted on the target gene candidates of DEMs through ClusterProfile (for GO analysis) and KOBAS (for KEGG analysis) software. miRNA library construction and deep sequencing were performed by Novogene (Beijing, China).
Techniques: Biomarker Discovery, Expressing, Over Expression, Inhibition, Binding Assay, Luciferase, Reporter Assay, Derivative Assay
