Recombinant:Article Title: Metabolic Control of Gametophore Shoot Formation through Arginine in the Moss Physcomitrium patens.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains E. coli DH10B Widely distributed N/A E. coli DH5a Widely distributed N/A E. coli DB3.1 Widely distributed N/A Chemicals, Peptides, and Recombinant Proteins Agar Sigma-Aldrich Cat# A1296 Cellulose film (Cellophane) Futamura Chemical Cat# P-5 6-Benzylaminopurine (BAP) Sigma-Aldrich Cat# B3408 b-Estradiol Tokyo Chemical Industry Cat# E0025 Dimethyl sulfoxide Tokyo Chemical Industry Cat# D0798 L-Arginine Wako Cat# 017-04612 EcoR V TaKaRa Cat# 1042A SmaI TaKaRa Cat# 1085A Not I TaKaRa Cat# 1166A Hind III TOYOBO Cat# HND-311 Apa I TaKaRa Cat# 1005A BstX I TaKaRa Cat# 1027A BamH I TaKaRa Cat# 1010A Xba I TaKaRa Cat# 1093A Sse8387 I TaKaRa Cat# 1183A Polyethylene glycol #6000 Nacalai tesque Cat# 10200-25 Geneticin (G418) Thermo Fisher Scientific Cat# 10131-035 Hygromycin B Thermo Fisher Scientific Cat# 10687010 Zeocine Thermo Fisher Scientific Cat# R25001 Blasticidin S, hydrochloride Funakoshi Cat# KK-400 Chloral hydrate Kanto Chemical Cat# 07208-01 13C15N L-Valine ISOTEC Cat# 600148 13C4 L-Malic acid ISOTEC Cat# 750484 Methanol Wako Cat# 132-06471 Formic acid Wako Cat# 067-04531 Critical Commercial Assays FastGene Plasmid Mini Kit Nippon Genetics Cat# FG-90402 FastGene Gel/PCR Extraction Kit Nippon Genetics Cat# FG-91202 RNeasy Plant Mini Kit QIAGEN Cat# 74904 ReverTra Ace qPCR RT Master Mix with gDNA Remover TOYOBO Cat# FSQ-301 THUNDERBIRD SYBR qPCR mix TOYOBO Cat# QPS-201 RNase-Free DNase Set QIAGEN Cat# 79254 AMPure XP Beckman Coulter Cat# A63882 TruSeq Stranded mRNA kit Illumina Cat# 20020594 Gateway pENTR/D-TOPO Thermo Fisher Scientific Cat# K240020 Gateway LR Clonase II Plus Enzyme Mix Thermo Fisher Scientific Cat# 12538-120 KOD -Plus- Neo TOYOBO Cat# KOD-401 KOD FX Neo TOYOBO Cat# KFX-201 (Continued on next page) e1 Cell Reports 32, 108127, September 8, 2020
Plasmid Preparation:Article Title: Metabolic Control of Gametophore Shoot Formation through Arginine in the Moss Physcomitrium patens.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains E. coli DH10B Widely distributed N/A E. coli DH5a Widely distributed N/A E. coli DB3.1 Widely distributed N/A Chemicals, Peptides, and Recombinant Proteins Agar Sigma-Aldrich Cat# A1296 Cellulose film (Cellophane) Futamura Chemical Cat# P-5 6-Benzylaminopurine (BAP) Sigma-Aldrich Cat# B3408 b-Estradiol Tokyo Chemical Industry Cat# E0025 Dimethyl sulfoxide Tokyo Chemical Industry Cat# D0798 L-Arginine Wako Cat# 017-04612 EcoR V TaKaRa Cat# 1042A SmaI TaKaRa Cat# 1085A Not I TaKaRa Cat# 1166A Hind III TOYOBO Cat# HND-311 Apa I TaKaRa Cat# 1005A BstX I TaKaRa Cat# 1027A BamH I TaKaRa Cat# 1010A Xba I TaKaRa Cat# 1093A Sse8387 I TaKaRa Cat# 1183A Polyethylene glycol #6000 Nacalai tesque Cat# 10200-25 Geneticin (G418) Thermo Fisher Scientific Cat# 10131-035 Hygromycin B Thermo Fisher Scientific Cat# 10687010 Zeocine Thermo Fisher Scientific Cat# R25001 Blasticidin S, hydrochloride Funakoshi Cat# KK-400 Chloral hydrate Kanto Chemical Cat# 07208-01 13C15N L-Valine ISOTEC Cat# 600148 13C4 L-Malic acid ISOTEC Cat# 750484 Methanol Wako Cat# 132-06471 Formic acid Wako Cat# 067-04531 Critical Commercial Assays FastGene Plasmid Mini Kit Nippon Genetics Cat# FG-90402 FastGene Gel/PCR Extraction Kit Nippon Genetics Cat# FG-91202 RNeasy Plant Mini Kit QIAGEN Cat# 74904 ReverTra Ace qPCR RT Master Mix with gDNA Remover TOYOBO Cat# FSQ-301 THUNDERBIRD SYBR qPCR mix TOYOBO Cat# QPS-201 RNase-Free DNase Set QIAGEN Cat# 79254 AMPure XP Beckman Coulter Cat# A63882 TruSeq Stranded mRNA kit Illumina Cat# 20020594 Gateway pENTR/D-TOPO Thermo Fisher Scientific Cat# K240020 Gateway LR Clonase II Plus Enzyme Mix Thermo Fisher Scientific Cat# 12538-120 KOD -Plus- Neo TOYOBO Cat# KOD-401 KOD FX Neo TOYOBO Cat# KFX-201 (Continued on next page) e1 Cell Reports 32, 108127, September 8, 2020
Real-time Polymerase Chain Reaction:Article Title: Metabolic Control of Gametophore Shoot Formation through Arginine in the Moss Physcomitrium patens.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains E. coli DH10B Widely distributed N/A E. coli DH5a Widely distributed N/A E. coli DB3.1 Widely distributed N/A Chemicals, Peptides, and Recombinant Proteins Agar Sigma-Aldrich Cat# A1296 Cellulose film (Cellophane) Futamura Chemical Cat# P-5 6-Benzylaminopurine (BAP) Sigma-Aldrich Cat# B3408 b-Estradiol Tokyo Chemical Industry Cat# E0025 Dimethyl sulfoxide Tokyo Chemical Industry Cat# D0798 L-Arginine Wako Cat# 017-04612 EcoR V TaKaRa Cat# 1042A SmaI TaKaRa Cat# 1085A Not I TaKaRa Cat# 1166A Hind III TOYOBO Cat# HND-311 Apa I TaKaRa Cat# 1005A BstX I TaKaRa Cat# 1027A BamH I TaKaRa Cat# 1010A Xba I TaKaRa Cat# 1093A Sse8387 I TaKaRa Cat# 1183A Polyethylene glycol #6000 Nacalai tesque Cat# 10200-25 Geneticin (G418) Thermo Fisher Scientific Cat# 10131-035 Hygromycin B Thermo Fisher Scientific Cat# 10687010 Zeocine Thermo Fisher Scientific Cat# R25001 Blasticidin S, hydrochloride Funakoshi Cat# KK-400 Chloral hydrate Kanto Chemical Cat# 07208-01 13C15N L-Valine ISOTEC Cat# 600148 13C4 L-Malic acid ISOTEC Cat# 750484 Methanol Wako Cat# 132-06471 Formic acid Wako Cat# 067-04531 Critical Commercial Assays FastGene Plasmid Mini Kit Nippon Genetics Cat# FG-90402 FastGene Gel/PCR Extraction Kit Nippon Genetics Cat# FG-91202 RNeasy Plant Mini Kit QIAGEN Cat# 74904 ReverTra Ace qPCR RT Master Mix with gDNA Remover TOYOBO Cat# FSQ-301 THUNDERBIRD SYBR qPCR mix TOYOBO Cat# QPS-201 RNase-Free DNase Set QIAGEN Cat# 79254 AMPure XP Beckman Coulter Cat# A63882 TruSeq Stranded mRNA kit Illumina Cat# 20020594 Gateway pENTR/D-TOPO Thermo Fisher Scientific Cat# K240020 Gateway LR Clonase II Plus Enzyme Mix Thermo Fisher Scientific Cat# 12538-120 KOD -Plus- Neo TOYOBO Cat# KOD-401 KOD FX Neo TOYOBO Cat# KFX-201 (Continued on next page) e1 Cell Reports 32, 108127, September 8, 2020
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