primary human gingival keratinocytes phgks  (ATCC)

Journal: Lasers in Medical Science

Article Title: Contrasting biological responses of gingival fibroblasts and keratinocyte to blue and violet light irradiation: implications for photobiomodulation use in the therapeutic management of periodontal disease

doi: 10.1007/s10103-026-04817-4

Figure Lengend Snippet: Influence of blue (457 nm, A-D) and violet (418 nm, E-F) light upon the metabolic activity and cytotoxicity of pHGFs (A, C and E, respectively) and pHGKs (B, D and F, respectively). Cells were irradiated with different fluences of blue and violet light. At 24 h post-treatment, the metabolic activity of the cells was assessed using AlamarBlue™. Metabolic activity was expressed as a percentage change compared to untreated controls; where the untreated controls were normalised to 100%, represented by the dashed line. LDH release, a marker of cytotoxicity, was also assessed 24 h post-treatment and cytotoxicity calculated as the percentage change in LDH levels compared to untreated controls, normalised to 0%. N = 3, mean ± SD (*p < 0.05, **p < 0.01, ****p < 0.0001)

Article Snippet: Primary human gingival fibroblasts (pHGFs) and primary human gingival keratinocytes (pHGKs) were sourced from ATCC/LGC Standards (Manassas, Virginia, USA). pHGF cells (healthy 60-year-old Caucasian female donor) were maintained in Dulbecco’s Modified Eagle Medium (DMEM), 10% foetal calf serum (FCS, ThermoFisher Scientific, Paisley, UK), 100 units/mL penicillin G sodium, 0.1 μg/mL streptomycin sulphate and 0.25 μg/mL amphotericin (ThermoFisher Scientific). pHGKs (healthy 65-year-old Caucasian male donor) and were maintained in Dermal Cell Basal Medium (DCBM, ATCC), supplemented with a Keratinocyte Growth Kit (ATCC).

Techniques: Activity Assay, Irradiation, Marker

Journal: Lasers in Medical Science

Article Title: Contrasting biological responses of gingival fibroblasts and keratinocyte to blue and violet light irradiation: implications for photobiomodulation use in the therapeutic management of periodontal disease

doi: 10.1007/s10103-026-04817-4

Figure Lengend Snippet: Assessment of ROS generation following the blue light (457 nm) treatment of pHGFs ( A ) and pHGKs ( B ). ROS levels were determined immediately following blue light treatment and the oxidation of added H 2 DCFDA resulting in the emittance of a green, fluorescent signal. ROS generation is reported as a value proportional to relative fluorescence units (RFU). N = 3, mean ± SD (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)

Article Snippet: Primary human gingival fibroblasts (pHGFs) and primary human gingival keratinocytes (pHGKs) were sourced from ATCC/LGC Standards (Manassas, Virginia, USA). pHGF cells (healthy 60-year-old Caucasian female donor) were maintained in Dulbecco’s Modified Eagle Medium (DMEM), 10% foetal calf serum (FCS, ThermoFisher Scientific, Paisley, UK), 100 units/mL penicillin G sodium, 0.1 μg/mL streptomycin sulphate and 0.25 μg/mL amphotericin (ThermoFisher Scientific). pHGKs (healthy 65-year-old Caucasian male donor) and were maintained in Dermal Cell Basal Medium (DCBM, ATCC), supplemented with a Keratinocyte Growth Kit (ATCC).

Techniques: Fluorescence

Journal: Lasers in Medical Science

Article Title: Contrasting biological responses of gingival fibroblasts and keratinocyte to blue and violet light irradiation: implications for photobiomodulation use in the therapeutic management of periodontal disease

doi: 10.1007/s10103-026-04817-4

Figure Lengend Snippet: Heatmaps depicting the fold changes in the gene expression of specific antioxidants (defined in Table ), proposed to be upregulated through ROS generation and measured at 6 h ( A , C ) and 24 h ( B , D ) in pHGFs and pHGKs, respectively. Relative gene expression is displayed as colours ranging from black to yellow, according to the scale, where the highest fold change is shown in yellow. Expression of target genes in untreated samples is represented by 1 on the colour scale (N = 3)

Article Snippet: Primary human gingival fibroblasts (pHGFs) and primary human gingival keratinocytes (pHGKs) were sourced from ATCC/LGC Standards (Manassas, Virginia, USA). pHGF cells (healthy 60-year-old Caucasian female donor) were maintained in Dulbecco’s Modified Eagle Medium (DMEM), 10% foetal calf serum (FCS, ThermoFisher Scientific, Paisley, UK), 100 units/mL penicillin G sodium, 0.1 μg/mL streptomycin sulphate and 0.25 μg/mL amphotericin (ThermoFisher Scientific). pHGKs (healthy 65-year-old Caucasian male donor) and were maintained in Dermal Cell Basal Medium (DCBM, ATCC), supplemented with a Keratinocyte Growth Kit (ATCC).

Techniques: Gene Expression, Expressing

Journal: Redox Biology

Article Title: Modification of the dermal matrix by senescence associated lipids and its functional consequence

doi: 10.1016/j.redox.2026.104069

Figure Lengend Snippet: Keratinocytes cultured on modified collagen type IV show oxidative stress and reduced MMP levels. A Scatter plots show gene expression levels of IL1A and HMOX1 relative to B2M in keratinocytes cultured on HNE or OxPAPC modified collagen type IV. Primary keratinocytes from two donors (f38y, m27y) were grown in quadruplicates for up to 72 h, n = 8. B Heatmap of log 2 -transformed relative expression levels normalized to the corresponding control. Transcriptional levels of senescence, inflammation, and keratinocyte differentiation markers were assessed. Colors represent expression changes (blue = downregulation, red = upregulation). Statistical significance was determined by one-way ANOVA and is indicated by asterisks (* p < 0.05; ** p < 0.01; *** p < 0.005; **** p < 0.001) with p-values shown for z values > 1.

Article Snippet: After gelation at 37 °C in a humidified atmosphere for 3 h in the absence of CO 2 , gels were equilibrated in Keratinocyte Growth Medium (KGM2, PromoCell, Germany) and placed into an incubator with 5% CO 2 .

Techniques: Cell Culture, Modification, Gene Expression, Transformation Assay, Expressing, Control

Journal: Redox Biology

Article Title: Modification of the dermal matrix by senescence associated lipids and its functional consequence

doi: 10.1016/j.redox.2026.104069

Figure Lengend Snippet: Skin equivalents with modified collagen show disturbed differentiation and early senescence. A Hematoxylin & Eosin staining of skin equivalents. Collagen was modified, fibroblasts from different donors (f34y, f42y, m25y) were seeded into the matrix, and keratinocytes (f49y, f35y, f30y) on top; n = 6. SE were cultured at the air-liquid interface for 5 days to allow differentiation. B Quantification of nuclei in stratum corneum, indicating parakeratosis, n = 10. Bar graph shows mean ± SD; significant differences determined by one-way ANOVA (* p < 0.05; ** p < 0.01; *** p < 0.005; **** p < 0.001). C Representative images of SE stained with anti -KRT10, anti -KRT14, and Hoechst nuclear counterstaining. D Boxplot showing quantification of epidermal thickness, n = 4. Data are represented as median ± interquartile range; significance was determined by one-way ANOVA. E Protein expression of cell cycle inhibition marker P16 and housekeeping marker TUBULIN from the epidermis of the SE. F Bar chart showing quantification of P16 vol intensity normalized to TUBULIN with mean ± SD, n = 4, differences determined by one-way ANOVA. G Scatter plots showing expression levels of LMNB1, CDKN1A , and HSPA1A relative to B2M from epidermis and dermis of SE, n = 6. Asterisks represent significant differences determined by one-way ANOVA. H Confocal images of SE with anti -LMNB1 immunostaining and Hoechst nuclear counterstaining. I Boxplot showing quantification of LaminB1 mean signal intensity per pixel, measured in a virtual cross section through the epidermal cells, n = 4, significance determined by one-way ANOVA. J Confocal images of SE with anti -γH2AX immunostaining and Hoechst counterstaining. K Bar chart showing quantification of γH2AX positive nuclei by tissue cytometry analysis of the entire tissue section, mean ± SD, n = 3. Significant differences (* p < 0.05; ** p < 0.01) determined by Student's t - test. Scale bars: A = 50 μm; C = 100 μm; H, J = 20 μm.

Article Snippet: After gelation at 37 °C in a humidified atmosphere for 3 h in the absence of CO 2 , gels were equilibrated in Keratinocyte Growth Medium (KGM2, PromoCell, Germany) and placed into an incubator with 5% CO 2 .

Techniques: Modification, Staining, Cell Culture, Expressing, Inhibition, Marker, Immunostaining, Cytometry