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anti keap1  (Proteintech)


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    Structured Review

    Proteintech anti keap1
    Anti Keap1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1342 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/keap1/KEAP1+Antibody/10__1038_slash_s41598___026___67384___2-77-0-4
    Average 96 stars, based on 1342 article reviews
    anti keap1 - by Bioz Stars, 2026-10
    96/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Ginsenoside Rh4 Triggers Ferroptosis in Lung Cancer: Targeting KEAP1 / NRF2 / HO-1 and Remodeling Gut Microbiota for Butyrate-Mediated ATF3 Activation
    Article Snippet: Protein samples were resolved on 10% SDS–polyacrylamide gels (UEIandy, Suzhou, China), transferred to PVDF membrane (Keygen BioTECH, Nanjing, China), and blocked with 5% bovine serum albumin. .. Primary antibodies against FTH1 (Sangon Biotech, Shanghai, China), GPX4 (Sangon Biotech, Shanghai, China), HO-1 (Proteintech, Wuhan, China), KEAP1 (Proteintech, Wuhan, China), NRF2 (Proteintech, Wuhan, China), SLC40A1 (Proteintech, Wuhan, China), SLC7A11 (Sangon Biotech, Shanghai, China), TFRC (Proteintech, Wuhan, China), and β-actin (Proteintech, Wuhan, China) were incubated with the membrane at 4 °C overnight. .. Following an hour of room temperature incubation with an HRP-conjugated goat anti-rabbit secondary antibody (Proteintech, Wuhan, China), protein bands were identified using a ChemiScope 5300 (Clinx Science Instruments Co., Ltd, Shanghai, China).

    Article Title: Ginsenoside Rh4 Triggers Ferroptosis in Lung Cancer: Targeting KEAP1 / NRF2 / HO-1 and Remodeling Gut Microbiota for Butyrate-Mediated ATF3 Activation.
    Article Snippet: Protein samples were resolved on 10% SDS– polyacrylamide gels (UEIandy, Suzhou, China), transferred to PVDF membrane (Keygen BioTECH, Nanjing, China), and blocked with 5% bovine serum albumin. .. Primary antibodies against FTH1 (Sangon Biotech, Shanghai, China), GPX4 (Sangon Biotech, Shanghai, China), HO-1 (Proteintech, Wuhan, China), KEAP1 (Proteintech, Wuhan, China), NRF2 (Proteintech, Wuhan, China), SLC40A1 (Proteintech, Wuhan, China), SLC7A11 (Sangon Biotech, Shanghai, China), TFRC (Proteintech, Wuhan, China), and β-actin (Proteintech, Wuhan, China) were incubated with the membrane at 4 ◦C overnight. .. Following an hour of room temperature incubation with an HRP-conjugated goat anti-rabbit secondary antibody (Proteintech, Wuhan, China), protein bands were identified using a ChemiScope 5300 (Clinx Science Instruments Co., Ltd, Shanghai, China).

    Article Title: Propyl gallate mitigates diabetic liver injury via suppressing SLC7A11/GPX4-mediated hepatic ferroptosis and modulating gut-liver axis.
    Article Snippet: .. The membranes were incubated with primary antibodies against Nrf2, Keap1, HO-1, NQO1, NF-κB p65, phospho-NF-κB p65, and I-κB (all from Cell Signaling Technology) and β-actin (Proteintech) was used as a loading control. .. After incubation with HRP-conjugated secondary anti-rabbit IgG antibodies (Proteintech), signals were visualized using enhanced chemiluminescence (ECL) reagents and imaged using a multifunctional gel documentation system.

    Membrane:

    Article Title: Ginsenoside Rh4 Triggers Ferroptosis in Lung Cancer: Targeting KEAP1 / NRF2 / HO-1 and Remodeling Gut Microbiota for Butyrate-Mediated ATF3 Activation
    Article Snippet: Protein samples were resolved on 10% SDS–polyacrylamide gels (UEIandy, Suzhou, China), transferred to PVDF membrane (Keygen BioTECH, Nanjing, China), and blocked with 5% bovine serum albumin. .. Primary antibodies against FTH1 (Sangon Biotech, Shanghai, China), GPX4 (Sangon Biotech, Shanghai, China), HO-1 (Proteintech, Wuhan, China), KEAP1 (Proteintech, Wuhan, China), NRF2 (Proteintech, Wuhan, China), SLC40A1 (Proteintech, Wuhan, China), SLC7A11 (Sangon Biotech, Shanghai, China), TFRC (Proteintech, Wuhan, China), and β-actin (Proteintech, Wuhan, China) were incubated with the membrane at 4 °C overnight. .. Following an hour of room temperature incubation with an HRP-conjugated goat anti-rabbit secondary antibody (Proteintech, Wuhan, China), protein bands were identified using a ChemiScope 5300 (Clinx Science Instruments Co., Ltd, Shanghai, China).

    Article Title: Ginsenoside Rh4 Triggers Ferroptosis in Lung Cancer: Targeting KEAP1 / NRF2 / HO-1 and Remodeling Gut Microbiota for Butyrate-Mediated ATF3 Activation.
    Article Snippet: Protein samples were resolved on 10% SDS– polyacrylamide gels (UEIandy, Suzhou, China), transferred to PVDF membrane (Keygen BioTECH, Nanjing, China), and blocked with 5% bovine serum albumin. .. Primary antibodies against FTH1 (Sangon Biotech, Shanghai, China), GPX4 (Sangon Biotech, Shanghai, China), HO-1 (Proteintech, Wuhan, China), KEAP1 (Proteintech, Wuhan, China), NRF2 (Proteintech, Wuhan, China), SLC40A1 (Proteintech, Wuhan, China), SLC7A11 (Sangon Biotech, Shanghai, China), TFRC (Proteintech, Wuhan, China), and β-actin (Proteintech, Wuhan, China) were incubated with the membrane at 4 ◦C overnight. .. Following an hour of room temperature incubation with an HRP-conjugated goat anti-rabbit secondary antibody (Proteintech, Wuhan, China), protein bands were identified using a ChemiScope 5300 (Clinx Science Instruments Co., Ltd, Shanghai, China).

    Western Blot:

    Article Title: A multimodal ROS logic-gated therapeutic platform disrupts the vicious cycle of senescence to promote aged bone defect repair
    Article Snippet: .. Western blotting was employed to evaluate protein expression of key targets, including Keap1 (Affinity, AF5266; 1:1000), Nrf2 (Proteintech, 16396-1-AP; 1:1000), Nqo1 (Abcam, ab80588; 1:10,000), Gclc (Proteintech, 12601-1-AP; 1:25000) and GAPDH (Proteintech, 60004-1-Ig; 1:50,000). ..

    Expressing:

    Article Title: A multimodal ROS logic-gated therapeutic platform disrupts the vicious cycle of senescence to promote aged bone defect repair
    Article Snippet: .. Western blotting was employed to evaluate protein expression of key targets, including Keap1 (Affinity, AF5266; 1:1000), Nrf2 (Proteintech, 16396-1-AP; 1:1000), Nqo1 (Abcam, ab80588; 1:10,000), Gclc (Proteintech, 12601-1-AP; 1:25000) and GAPDH (Proteintech, 60004-1-Ig; 1:50,000). ..

    Blocking Assay:

    Article Title: Tubuloside A attenuates sepsis-induced acute lung injury by modulating the NF-κB p50-Nrf2/GPX4 axis to suppress inflammation, oxidative stress and ferroptosis.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Control:

    Article Title: Propyl gallate mitigates diabetic liver injury via suppressing SLC7A11/GPX4-mediated hepatic ferroptosis and modulating gut-liver axis.
    Article Snippet: .. The membranes were incubated with primary antibodies against Nrf2, Keap1, HO-1, NQO1, NF-κB p65, phospho-NF-κB p65, and I-κB (all from Cell Signaling Technology) and β-actin (Proteintech) was used as a loading control. .. After incubation with HRP-conjugated secondary anti-rabbit IgG antibodies (Proteintech), signals were visualized using enhanced chemiluminescence (ECL) reagents and imaged using a multifunctional gel documentation system.



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    Santa Cruz Biotechnology keap1
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    A , gene expression of fission and fusion markers Fis1, Drp1, <t>OPA1</t> and MFN2 in FXN‐cKO and FXNKD hearts normalized to controls. B , western blot images of the corresponding proteins involved in mitochondrial fission–fusion processes. C , protein expression of fission and fusion markers Fis1, Drp1, OPA1 and MFN2 in FXN‐cKO and FXNKD mice normalized to controls. D , protein expression of OPA1‐l/OPA1‐s ratio in FXN‐cKO hearts. E , OPA1‐l/OPA1‐s ratio in FXNKD hearts. F , schematic representation of fusion and fission. N = 8 mouse hearts per genotype for gene expression and n = 4 mouse hearts per genotype for protein expression. Numbers above the brackets indicate the exact P ‐value.
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    Proteintech kelchlike ech associated protein 1
    A , gene expression of fission and fusion markers Fis1, Drp1, <t>OPA1</t> and MFN2 in FXN‐cKO and FXNKD hearts normalized to controls. B , western blot images of the corresponding proteins involved in mitochondrial fission–fusion processes. C , protein expression of fission and fusion markers Fis1, Drp1, OPA1 and MFN2 in FXN‐cKO and FXNKD mice normalized to controls. D , protein expression of OPA1‐l/OPA1‐s ratio in FXN‐cKO hearts. E , OPA1‐l/OPA1‐s ratio in FXNKD hearts. F , schematic representation of fusion and fission. N = 8 mouse hearts per genotype for gene expression and n = 4 mouse hearts per genotype for protein expression. Numbers above the brackets indicate the exact P ‐value.
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    Image Search Results


    A , gene expression of fission and fusion markers Fis1, Drp1, OPA1 and MFN2 in FXN‐cKO and FXNKD hearts normalized to controls. B , western blot images of the corresponding proteins involved in mitochondrial fission–fusion processes. C , protein expression of fission and fusion markers Fis1, Drp1, OPA1 and MFN2 in FXN‐cKO and FXNKD mice normalized to controls. D , protein expression of OPA1‐l/OPA1‐s ratio in FXN‐cKO hearts. E , OPA1‐l/OPA1‐s ratio in FXNKD hearts. F , schematic representation of fusion and fission. N = 8 mouse hearts per genotype for gene expression and n = 4 mouse hearts per genotype for protein expression. Numbers above the brackets indicate the exact P ‐value.

    Journal: The Journal of Physiology

    Article Title: Mitochondrial dynamics and repair differences in two mouse models of Friedreich's ataxia

    doi: 10.1113/JP291356

    Figure Lengend Snippet: A , gene expression of fission and fusion markers Fis1, Drp1, OPA1 and MFN2 in FXN‐cKO and FXNKD hearts normalized to controls. B , western blot images of the corresponding proteins involved in mitochondrial fission–fusion processes. C , protein expression of fission and fusion markers Fis1, Drp1, OPA1 and MFN2 in FXN‐cKO and FXNKD mice normalized to controls. D , protein expression of OPA1‐l/OPA1‐s ratio in FXN‐cKO hearts. E , OPA1‐l/OPA1‐s ratio in FXNKD hearts. F , schematic representation of fusion and fission. N = 8 mouse hearts per genotype for gene expression and n = 4 mouse hearts per genotype for protein expression. Numbers above the brackets indicate the exact P ‐value.

    Article Snippet: Primary antibodies: TFAM (Cell Signalling Technology, 8076), DRP1 (Cell Signalling Technology, 8570), FIS1 (Cell Signalling Technology, 32525), MFN2 (Abcam, ab56889), OPA1 (Cell Signalling Technology, 8047), Pink1 (Abcam, 23707), Parkin (Cell Signalling Technology, 32833), p62 (Abcam, ab56416), FUNDC1 (Cell Signalling Technology, 49240), BNIP3L (Cell Signalling Technology, 12396), LC3 (Cell Signalling Technology, 12 741), ATG7 (Cell Signalling Technology, 8558), ATG12–5 (Cell Signalling Technology, 4180), LAMP1 (Abcam, 24170), CD63 (Cell Signalling Technology, 10112), Cathepsin D (Cell Signalling, 74089), GAPDH (Abcam, ab8245), αTubulin (Millipore Sigma, T6199).

    Techniques: Gene Expression, Western Blot, Expressing