Journal: iScience
Article Title: Benzyl isothiocyanate suppresses development of thyroid carcinoma by regulating both autophagy and apoptosis pathway
doi: 10.1016/j.isci.2024.110796
Figure Lengend Snippet: Effect of BITC on inflammation-associated cancer in 8305C cells (A) BITC treatments at the concentrations of 100 μM and 200 μM was able to significantly reduce the expression of TRAF6, compared to 8305C cells. ∗∗ p < 0.01 vs. 8305C; ∗ p < 0.05 vs. 8305C. (B and C) The enzyme-linked immunosorbent assay (ELISA) revealed a significant decrease of IL-12p70, following BITC treatments at all the concentrations (C) and of IL-17 levels, especially at the concentration of 100 μM (B) ∗ p < 0.05 vs. 8505C; ∗∗ p < 0.01 vs. 8305C. Values are means ± SEM. We used one-way ANOVA test followed by Bonferroni post hoc test for multiple comparisons.
Article Snippet: To evaluate the inflammatory response, the levels of IL-17 (ELISA kit for IL-17, SEA063Mu Cloud-Clone Corp.), IL-12p70 (Human IL-12p70 ELISA kit, KE10014, Proteintech), Cyclin A (cat ABIN6955143 Biocompare) and CDK2 (ab316258 – Human CDK2 SimpleStep ELISA Kit) were measured in cell lysates collected by enzyme-linked immunosorbent assay (ELISA), according to the manufacturer’s instructions.
Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Concentration Assay