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plasmid myc ddk kv4 2  (OriGene)


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    Structured Review

    OriGene plasmid myc ddk kv4 2
    Plasmid Myc Ddk Kv4 2, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kcnd2/Kv4%2E2+(KCND2)+(NM_012281)+Human+Tagged+ORF+Clone/pmc12232208-1165-0-3
    Average 93 stars, based on 8 article reviews
    plasmid myc ddk kv4 2 - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Activity-dependent degradation of Kv4.2 contributes to synaptic plasticity and behavior in Angelman syndrome model mice
    Article Snippet: .. Plasmid: Myc-DDK-Kv4.2 , Origene , Cat#RC215266. .. Plasmid: Myc-DDK-DPP6 , Origene , Cat#RC216919.

    Construct:

    Article Title: Identification of Kv4.2 protein complex and modifications by tandem affinity purification-mass spectrometry in primary neurons
    Article Snippet: OPTI-MEM (Gibco 31985), Chloroquine (Sigma C6628), 0.45 μm low protein binding filter (Corning 430768), Opti-prep density gradient medium (Sigma D1556-250 mL), Poly-L-Lysine (Sigma P-2636), Trizma buffer pH 8.5 0.1 M (Sigma T1194), B27 supplements (Gibco 17504044), Papain (Worthington LS003119), Characterized Fetal Bovine Serum (Hyclone SH30071.03), 10 × HBSS (Gibco 14185-052), Pen/strep (Gibco 15140122), Pyruvate (Gibco 11360070), Hepes (Gibco 15630080), Glucose (Sigma G8270), Ara C (Sigma C-6645), DNase (Sigma DN-25), Neurobasal Media (Gibco 21103-049), Triton X-100 (Sigma T8787), Deoxycholate (Sigma D6750), Sample loading buffer (Invitrogen NP0007), Sample reducing agent (Invitrogen NP0009), phosSTOP (Roche, 04906837001), Complete EDTA–Free protease inhibitors (Roche 56079200), Silver staining (Invitrogen LC6070), and Coomassie staining (LC6060). .. The human Myc-DDK-Kv4.2 construct was purchased from Origene (RC215266). .. Kv4.2 was subcloned into the TAP tag vector that was obtained from Agilent (pCTAP, #240102).

    Western Blot:

    Article Title: Activation of histamine type 2 receptors enhances intrinsic excitability of medium spiny neurons in the nucleus accumbens
    Article Snippet: .. Primary antibodies for anti-Kv4.2 (WB 1:1000, monoclonal, Origene 75-016), anti-H1R (WB 1:1000, monoclonal, Santa Cruz, sc-374621; Santa Cruz, CA, USA), anti-H2R (WB 1:1000, goat, Everest Biotech, EB06905; Bicester, UK), anti-beta-actin (WB 1:2000, polyclonal, Abcam ab8227; Cambridge, MA, USA) and anti-PSD95 (WB 1:1000, polyclonal, Cell Signaling 3550; Danvers, MA, USA) were incubated overnight and revealed with HRP-conjugated secondary antibodies (Cell Signaling Technology). .. Expression was evaluated and documented by using UVItec Cambridge Alliance.

    Incubation:

    Article Title: Activation of histamine type 2 receptors enhances intrinsic excitability of medium spiny neurons in the nucleus accumbens
    Article Snippet: .. Primary antibodies for anti-Kv4.2 (WB 1:1000, monoclonal, Origene 75-016), anti-H1R (WB 1:1000, monoclonal, Santa Cruz, sc-374621; Santa Cruz, CA, USA), anti-H2R (WB 1:1000, goat, Everest Biotech, EB06905; Bicester, UK), anti-beta-actin (WB 1:2000, polyclonal, Abcam ab8227; Cambridge, MA, USA) and anti-PSD95 (WB 1:1000, polyclonal, Cell Signaling 3550; Danvers, MA, USA) were incubated overnight and revealed with HRP-conjugated secondary antibodies (Cell Signaling Technology). .. Expression was evaluated and documented by using UVItec Cambridge Alliance.

    other:


    Recombinant:

    Article Title: R-type voltage-gated Ca 2+ channels mediate A-type K + current regulation of synaptic input in hippocampal dendrites.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat anti-Guinea Pig, Alexa Fluor 488 ThermoFisher Cat#A11073; RRID: AB_2534117 Goat anti-Rabbit, Alexa Fluor 488 ThermoFisher Cat#A11008; RRID: AB_143165 Goat anti-Mouse, Alexa Fluor 555 ThermoFisher Cat#A21422; RRID: AB_2535844 Goat anti-Mouse, Alexa Fluor 647 ThermoFisher Cat#A21236; RRID: AB_2535805 Goat anti-Mouse, Alexa Fluor 680 ThermoFisher Cat#A21057; RRID: AB_2535723 Goat anti-Rabbit, IRDye 800CW Li-Cor Biosciences Cat#926-32211; RRID: AB_2651127 Guinea Pig anti-Cav2.3 Akos Kulik (Parajuli et al., 2012) N/A Mouse anti-Cav2.3 (clone 62C10) Synaptic Systems Cat#152-441; RRID: AB_2619847 Mouse anti-Kv4.2 (clone K57/1) NeuroMab Cat#75-016; RRID: AB_2877281 Mouse anti-Myc (clone 9E10) Millipore Sigma Cat#M4439; RRID: AB_439694 Rabbit anti-GFP ThermoFisher Cat#A11122; RRID: AB_221569 Chemicals, peptides, and recombinant proteins AmmTx3 Alamone Labs Cat#STA-305 Tetrodotoxin Tocris Cat#1069 SR 95531 hydrobromide (Gabazine) Tocris Cat#1262 MK-801 maleate Tocris Cat#0924 CNQX Tocris Cat#1045 D-AP5 Tocris Cat#0106 Critical commercial assays TAP Purification Kit Agilent Technologies Cat#240107 Experimental models: Cell lines Homo sapiens: HEK293-FT ThermoFisher R70007; RRID: CVCL_6911 Cercopithecus aethiops: COS-7 ATCC Cat#CRL-1651; RRID: CVCL_0224 Experimental models: Organisms/strains Mouse: C57BL/6J Jackson Laboratory Cat#0006664; RRID: IMSR_JAX:000664 Mouse: Cav2.3-/- Richard Miller (Wilson et al., 2000) N/A Rat: Sprague Dawley Taconic Biosciences Cat#Sprague Dawley , SD Recombinant DNA Cav2.3-GFP Ehud Isacoff; (Ulbrich and Isacoff, 2007) CCDS: 55664.1 YFP-Cav2.3 This paper CCDS: 55664.1 pcDNA3-DPP6S Bernardo Rudy N/A Kv4.2-GFP Dax Hoffman; (Kim et al., 2007) CCDS: 29974.1 Kv4.2-CFP This paper CCDS: 29974.1 Kv4.2-YFP This paper CCDS: 29974.1 Kv4.2-SGFP2 This paper CCDS: 29974.1 Kv4.2-Myc-DDK Origene Cat#RC215266 pCMV-KChIP2c Paul Pfaffinger GenBank: AF269285.1 KChIP2c-EFmut This paper N/A KChIP2c-CFP This paper GenBank: AF269285.1 AKAP79-YFP Mark Dell’Acqua (Oliveria et al., 2003) GenBank: NP_004848.3 (Continued on next page) e1 Cell Reports 38, 110264, January 18, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER PKARII-CFP Mark Dell’Acqua (Oliveria et al., 2003) GenBank: NP_032950.1 GCaMP6f Douglas Kim & GENIE Project Addgene 40755 CFP-18aa-YFP Clemens Kaminski (Kaminski et al., 2014) N/A SGFP2-C1 Dorus Gadella; (Kremers et al., 2007) Addgene 22881 SGFP2-N1 This paper N/A mCherry-N1 Takara Bio Cat#632523 Software and algorithms Igor Pro WaveMetrics, Inc. www.wavemetrics.com Imagej Schneider et al., 2012 imagej.nih.gov/ij pCLAMP Molecular Devices www.moleculardevices.com Prism 9 GraphPad Software, LLC. www.graphpad.com

    Purification:

    Article Title: R-type voltage-gated Ca 2+ channels mediate A-type K + current regulation of synaptic input in hippocampal dendrites.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat anti-Guinea Pig, Alexa Fluor 488 ThermoFisher Cat#A11073; RRID: AB_2534117 Goat anti-Rabbit, Alexa Fluor 488 ThermoFisher Cat#A11008; RRID: AB_143165 Goat anti-Mouse, Alexa Fluor 555 ThermoFisher Cat#A21422; RRID: AB_2535844 Goat anti-Mouse, Alexa Fluor 647 ThermoFisher Cat#A21236; RRID: AB_2535805 Goat anti-Mouse, Alexa Fluor 680 ThermoFisher Cat#A21057; RRID: AB_2535723 Goat anti-Rabbit, IRDye 800CW Li-Cor Biosciences Cat#926-32211; RRID: AB_2651127 Guinea Pig anti-Cav2.3 Akos Kulik (Parajuli et al., 2012) N/A Mouse anti-Cav2.3 (clone 62C10) Synaptic Systems Cat#152-441; RRID: AB_2619847 Mouse anti-Kv4.2 (clone K57/1) NeuroMab Cat#75-016; RRID: AB_2877281 Mouse anti-Myc (clone 9E10) Millipore Sigma Cat#M4439; RRID: AB_439694 Rabbit anti-GFP ThermoFisher Cat#A11122; RRID: AB_221569 Chemicals, peptides, and recombinant proteins AmmTx3 Alamone Labs Cat#STA-305 Tetrodotoxin Tocris Cat#1069 SR 95531 hydrobromide (Gabazine) Tocris Cat#1262 MK-801 maleate Tocris Cat#0924 CNQX Tocris Cat#1045 D-AP5 Tocris Cat#0106 Critical commercial assays TAP Purification Kit Agilent Technologies Cat#240107 Experimental models: Cell lines Homo sapiens: HEK293-FT ThermoFisher R70007; RRID: CVCL_6911 Cercopithecus aethiops: COS-7 ATCC Cat#CRL-1651; RRID: CVCL_0224 Experimental models: Organisms/strains Mouse: C57BL/6J Jackson Laboratory Cat#0006664; RRID: IMSR_JAX:000664 Mouse: Cav2.3-/- Richard Miller (Wilson et al., 2000) N/A Rat: Sprague Dawley Taconic Biosciences Cat#Sprague Dawley , SD Recombinant DNA Cav2.3-GFP Ehud Isacoff; (Ulbrich and Isacoff, 2007) CCDS: 55664.1 YFP-Cav2.3 This paper CCDS: 55664.1 pcDNA3-DPP6S Bernardo Rudy N/A Kv4.2-GFP Dax Hoffman; (Kim et al., 2007) CCDS: 29974.1 Kv4.2-CFP This paper CCDS: 29974.1 Kv4.2-YFP This paper CCDS: 29974.1 Kv4.2-SGFP2 This paper CCDS: 29974.1 Kv4.2-Myc-DDK Origene Cat#RC215266 pCMV-KChIP2c Paul Pfaffinger GenBank: AF269285.1 KChIP2c-EFmut This paper N/A KChIP2c-CFP This paper GenBank: AF269285.1 AKAP79-YFP Mark Dell’Acqua (Oliveria et al., 2003) GenBank: NP_004848.3 (Continued on next page) e1 Cell Reports 38, 110264, January 18, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER PKARII-CFP Mark Dell’Acqua (Oliveria et al., 2003) GenBank: NP_032950.1 GCaMP6f Douglas Kim & GENIE Project Addgene 40755 CFP-18aa-YFP Clemens Kaminski (Kaminski et al., 2014) N/A SGFP2-C1 Dorus Gadella; (Kremers et al., 2007) Addgene 22881 SGFP2-N1 This paper N/A mCherry-N1 Takara Bio Cat#632523 Software and algorithms Igor Pro WaveMetrics, Inc. www.wavemetrics.com Imagej Schneider et al., 2012 imagej.nih.gov/ij pCLAMP Molecular Devices www.moleculardevices.com Prism 9 GraphPad Software, LLC. www.graphpad.com



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