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jijoye  (ATCC)


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    Structured Review

    ATCC jijoye
    (A) CCND2 sgRNA abundances in Brunello P3HR-1 and GM12878 dropout screens. Log 2 CCND2 sgRNA abundances from GM12878 (red) and P3HR-1 (blue) cells 21 days post library selection were determined via deep sequencing and compared to input library sgRNA abundance (green). (B) CCND2 transcript levels during EBV-mediated B cell transformation. RNAseq was performed on primary B cells infected with B95-8 virus on indicated days post infection (dpi). Normalized reads for CCND2 are shown. (C) Immunoblot analysis of CCND2 levels in response to LMP1 <t>expression.</t> <t>Daudi</t> Burkitt cells harboring selectively inducible WT or transformation effector site (TES) mutant LMP1 cassettes were mock induced or treated with 250 ng / mL doxycycline for 24 hours. Cells were then harvested and lysates analyzed via immunoblot with indicated antibodies. (D) Effect of JunB knockout on CCND2 levels. Cas9+ P3HR-1, <t>Jijoye,</t> or GM12878 cells expressing control or JunB sgRNAs. Seven days post selection, WCL was extracted from cells and immunoblot performed using indicated antibodies. P-values were determined by one-sided Fisher’s exact test. * p<0.05, **p<0.005, ***p<0.0005
    Jijoye, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1753 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/jijoye/product/ATCC
    Average 98 stars, based on 1753 article reviews
    jijoye - by Bioz Stars, 2026-05
    98/100 stars

    Images

    1) Product Images from "CRISPR Screens Reveal Epstein-Barr Virus-activated JunB as a Key Lymphoblastoid B cell Dependency Factor that Represses Cyclin Dependent Kinase Inhibitor P18INK4c"

    Article Title: CRISPR Screens Reveal Epstein-Barr Virus-activated JunB as a Key Lymphoblastoid B cell Dependency Factor that Represses Cyclin Dependent Kinase Inhibitor P18INK4c

    Journal: bioRxiv

    doi: 10.64898/2026.03.31.715635

    (A) CCND2 sgRNA abundances in Brunello P3HR-1 and GM12878 dropout screens. Log 2 CCND2 sgRNA abundances from GM12878 (red) and P3HR-1 (blue) cells 21 days post library selection were determined via deep sequencing and compared to input library sgRNA abundance (green). (B) CCND2 transcript levels during EBV-mediated B cell transformation. RNAseq was performed on primary B cells infected with B95-8 virus on indicated days post infection (dpi). Normalized reads for CCND2 are shown. (C) Immunoblot analysis of CCND2 levels in response to LMP1 expression. Daudi Burkitt cells harboring selectively inducible WT or transformation effector site (TES) mutant LMP1 cassettes were mock induced or treated with 250 ng / mL doxycycline for 24 hours. Cells were then harvested and lysates analyzed via immunoblot with indicated antibodies. (D) Effect of JunB knockout on CCND2 levels. Cas9+ P3HR-1, Jijoye, or GM12878 cells expressing control or JunB sgRNAs. Seven days post selection, WCL was extracted from cells and immunoblot performed using indicated antibodies. P-values were determined by one-sided Fisher’s exact test. * p<0.05, **p<0.005, ***p<0.0005
    Figure Legend Snippet: (A) CCND2 sgRNA abundances in Brunello P3HR-1 and GM12878 dropout screens. Log 2 CCND2 sgRNA abundances from GM12878 (red) and P3HR-1 (blue) cells 21 days post library selection were determined via deep sequencing and compared to input library sgRNA abundance (green). (B) CCND2 transcript levels during EBV-mediated B cell transformation. RNAseq was performed on primary B cells infected with B95-8 virus on indicated days post infection (dpi). Normalized reads for CCND2 are shown. (C) Immunoblot analysis of CCND2 levels in response to LMP1 expression. Daudi Burkitt cells harboring selectively inducible WT or transformation effector site (TES) mutant LMP1 cassettes were mock induced or treated with 250 ng / mL doxycycline for 24 hours. Cells were then harvested and lysates analyzed via immunoblot with indicated antibodies. (D) Effect of JunB knockout on CCND2 levels. Cas9+ P3HR-1, Jijoye, or GM12878 cells expressing control or JunB sgRNAs. Seven days post selection, WCL was extracted from cells and immunoblot performed using indicated antibodies. P-values were determined by one-sided Fisher’s exact test. * p<0.05, **p<0.005, ***p<0.0005

    Techniques Used: Selection, Sequencing, Transformation Assay, RNA sequencing, Infection, Virus, Western Blot, Expressing, Mutagenesis, Knock-Out, Control



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    Image Search Results


    (A) CCND2 sgRNA abundances in Brunello P3HR-1 and GM12878 dropout screens. Log 2 CCND2 sgRNA abundances from GM12878 (red) and P3HR-1 (blue) cells 21 days post library selection were determined via deep sequencing and compared to input library sgRNA abundance (green). (B) CCND2 transcript levels during EBV-mediated B cell transformation. RNAseq was performed on primary B cells infected with B95-8 virus on indicated days post infection (dpi). Normalized reads for CCND2 are shown. (C) Immunoblot analysis of CCND2 levels in response to LMP1 expression. Daudi Burkitt cells harboring selectively inducible WT or transformation effector site (TES) mutant LMP1 cassettes were mock induced or treated with 250 ng / mL doxycycline for 24 hours. Cells were then harvested and lysates analyzed via immunoblot with indicated antibodies. (D) Effect of JunB knockout on CCND2 levels. Cas9+ P3HR-1, Jijoye, or GM12878 cells expressing control or JunB sgRNAs. Seven days post selection, WCL was extracted from cells and immunoblot performed using indicated antibodies. P-values were determined by one-sided Fisher’s exact test. * p<0.05, **p<0.005, ***p<0.0005

    Journal: bioRxiv

    Article Title: CRISPR Screens Reveal Epstein-Barr Virus-activated JunB as a Key Lymphoblastoid B cell Dependency Factor that Represses Cyclin Dependent Kinase Inhibitor P18INK4c

    doi: 10.64898/2026.03.31.715635

    Figure Lengend Snippet: (A) CCND2 sgRNA abundances in Brunello P3HR-1 and GM12878 dropout screens. Log 2 CCND2 sgRNA abundances from GM12878 (red) and P3HR-1 (blue) cells 21 days post library selection were determined via deep sequencing and compared to input library sgRNA abundance (green). (B) CCND2 transcript levels during EBV-mediated B cell transformation. RNAseq was performed on primary B cells infected with B95-8 virus on indicated days post infection (dpi). Normalized reads for CCND2 are shown. (C) Immunoblot analysis of CCND2 levels in response to LMP1 expression. Daudi Burkitt cells harboring selectively inducible WT or transformation effector site (TES) mutant LMP1 cassettes were mock induced or treated with 250 ng / mL doxycycline for 24 hours. Cells were then harvested and lysates analyzed via immunoblot with indicated antibodies. (D) Effect of JunB knockout on CCND2 levels. Cas9+ P3HR-1, Jijoye, or GM12878 cells expressing control or JunB sgRNAs. Seven days post selection, WCL was extracted from cells and immunoblot performed using indicated antibodies. P-values were determined by one-sided Fisher’s exact test. * p<0.05, **p<0.005, ***p<0.0005

    Article Snippet: 293T, Daudi and Jijoye were purchased from American Type Culture Collection.

    Techniques: Selection, Sequencing, Transformation Assay, RNA sequencing, Infection, Virus, Western Blot, Expressing, Mutagenesis, Knock-Out, Control

    Viral exmiRs identify viral reactivation in cells (A–C) RT-dPCR quantification of cellular EBV genes EBNA1 (i) and BZLF1 (ii) at 6 and 24 h post-chemical induction of EBV-harboring B cells Jijoye (A), Raji (B), and Daudi (C). (D) Real-time RT-qPCR quantification of EBV miR-BHRF1-2-3p in spent media of non-induced and 24 h post-induction Jijoye (i), Raji (ii), and Daudi (iii) cells. (E–H) RT-dPCR quantification of cellular HHV-8 genes LANA and Rta at 6 and 24 h post-chemical induction of BC-1 (E) and BC-3 (G) cells. Real-time RT-qPCR quantification of HHV-8 miR-K12-10a-3p (i), miR-K12-10b (ii), and miR-K12-12-3p (iii) from spent media of non-induced and 24 h post-induction BC-1 (F) and BC-3 (H) cells. Data shown are from 4 biological repeats. Error bars represent the standard error of the mean.

    Journal: Molecular Therapy. Nucleic Acids

    Article Title: Extracellular viral microRNAs as biomarkers of virus infection in human cells

    doi: 10.1016/j.omtn.2024.102444

    Figure Lengend Snippet: Viral exmiRs identify viral reactivation in cells (A–C) RT-dPCR quantification of cellular EBV genes EBNA1 (i) and BZLF1 (ii) at 6 and 24 h post-chemical induction of EBV-harboring B cells Jijoye (A), Raji (B), and Daudi (C). (D) Real-time RT-qPCR quantification of EBV miR-BHRF1-2-3p in spent media of non-induced and 24 h post-induction Jijoye (i), Raji (ii), and Daudi (iii) cells. (E–H) RT-dPCR quantification of cellular HHV-8 genes LANA and Rta at 6 and 24 h post-chemical induction of BC-1 (E) and BC-3 (G) cells. Real-time RT-qPCR quantification of HHV-8 miR-K12-10a-3p (i), miR-K12-10b (ii), and miR-K12-12-3p (iii) from spent media of non-induced and 24 h post-induction BC-1 (F) and BC-3 (H) cells. Data shown are from 4 biological repeats. Error bars represent the standard error of the mean.

    Article Snippet: EBV type 2 was produced by treating the Jijoye cell line (ATCC CCL-87) with 4 mM sodium butyrate and 25 ng/mL TPA and concentrated by ultracentrifugation.

    Techniques: Quantitative RT-PCR