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jc 1 kit  (Beyotime)


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    Structured Review

    Beyotime jc 1 kit
    Jc 1 Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 14998 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/jc+1+kit/JC-1/pmc13084373-92-7-10
    Average 99 stars, based on 14998 article reviews
    jc 1 kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Membrane:

    Article Title: Three-dimensional printed PCL/nHA scaffolds promote soft tissue functional fibrosis to repair chest wall defect via Piezo1/Ca 2+ signal during respiratory motion
    Article Snippet: Intracellular reactive oxygen species (ROS) levels were assessed using a ROS detection kit (S0033S, Beyotime, China). .. Mitochondrial membrane potential was evaluated using the JC-1 kit (C2006, Beyotime, China). ..

    Article Title: IDH2 lactylation regulates mitochondrial dysfunction injury induced by myocardial ischemia-reperfusion via the AMPK signaling pathway
    Article Snippet: The ATP content was determined using an assay kit (cat. no. S0026; Beyotime Institute of Biotechnology) according to the manufacturer's instructions. .. Mitochondrial membrane potential was measured with a JC-1 kit (cat. no. C2003S; Beyotime Institute of Biotechnology). ..

    Article Title: Cystatin B Attenuates Cerebral Ischemia Reperfusion Injury by Inhibiting the JAK2/STAT3 Signaling Pathway.
    Article Snippet: .. Changes in mitochondrial membrane potential, another key indicator of apoptosis, were detected with the JC- 1 kit (C2006, Beyotime) following the manufacturer's protocol. .. All fluorescent images were acquired using a fluorescence microscope (BX51, OLYMPUS) and analyzed with IPP software.

    Article Title: Orchestrating diabetic wound repair via mitochondria-targeted delivery of dihydromyricetin with tailored ADSC-derived biohybrid nanovesicles
    Article Snippet: The cell viability in oxidative stress was assessed by ratio of dead to live cells using a Calcein-AM/PI kit (Beyotime, China). .. Mitochondrial membrane potential was quantified with an enhanced JC-1 kit (Beyotime, China). .. The mitochondrial Ca 2+ level was determined with a Rhod-2/AM kit (Yeasen, China).

    H&E Stain:

    Article Title: Divaroside alleviates barium chloride-induced arrhythmia by activating the Nrf2/HO-1 axis to modulate autophagy and calcium homeostasis.
    Article Snippet: Background: Acanthopanax sessiliflorus (A. sessiliflorus) extract has been used in various traditional Chinese medicines and compound preparations for the treatment of arrhythmia.. Our preliminary studies have demonstrated that A. sessiliflorus extract exerts significant protective effects against arrhythmia induced by barium chloride.. The present study aims to elucidate the bioactive components of A. sessiliflorus and to investigate the underlying mechanisms by which it mitigates barium chloride-induced arrhythmia.

    Bicinchoninic Acid Protein Assay:

    Article Title: BDH2 Inhibits Lung Adenocarcinoma Metastasis by Promoting Ferroptosis.
    Article Snippet: Ferrostatin-1 (SML0583, Sigma-Aldrich, USA) and Erastin (SML2289, Sigma-Aldrich, USA) were used as ferroptosis inhibitors and inducers, respectively. .. Most generalpurpose reagents, including RIPA buffer (P0013B, Beyotime, Shanghai, China), BCA protein assay kit (P0012, Beyotime, China), enhanced chemiluminescence (ECL) detection kit (P0018FS, Beyotime, China), and JC-1 kit (C2006, Beyotime, China), were purchased from Beyotime. .. The Fe2– assay kit (E1042, Beyotime, China), malondialdehyde (MDA) assay kit (S0131S, Beyotime, China), and reactive oxygen species (ROS) detection kit (S0033M, Beyotime, China) were used for biochemical analyses.

    Multiple Displacement Amplification:

    Article Title: Multifunctional armored nanoemulsion of elemene combining ferroptosis induction and gut homeostasis restoration in colorectal cancer therapy
    Article Snippet: D-Luciferin potassium salt, and fluorescent 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine-4-chlorobenzene sulfonate (DiD), coumarin-6 (C6), and Liproxstatin-1 (Lip-1, purity >99%) were purchased from Sigma Aldrich. .. CM-H2DCFDA kit, BODIPY 581/591 C11kit, JC-1 kit, MDA kit, GSH kit, GSH-PX kit, mouse TNF-α kit, and mouse IFN-γ ELISA kit were purchased from Beyotime Biotechnology, Shanghai, China. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Multifunctional armored nanoemulsion of elemene combining ferroptosis induction and gut homeostasis restoration in colorectal cancer therapy
    Article Snippet: D-Luciferin potassium salt, and fluorescent 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine-4-chlorobenzene sulfonate (DiD), coumarin-6 (C6), and Liproxstatin-1 (Lip-1, purity >99%) were purchased from Sigma Aldrich. .. CM-H2DCFDA kit, BODIPY 581/591 C11kit, JC-1 kit, MDA kit, GSH kit, GSH-PX kit, mouse TNF-α kit, and mouse IFN-γ ELISA kit were purchased from Beyotime Biotechnology, Shanghai, China. ..



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    IPA protects mBMSCs against H 2 O 2 -induced apoptotic injury. (A) Effects of IPA on mBMSC viability over 1, 2, and 3 days, as determined by the CCK-8 assay. (B) Effects of IPA pretreatment on mBMSC viability following H 2 O 2 exposure for 12, 24, and 48 h, as determined by the CCK-8 assay. (C) Live/dead staining of mBMSCs on day 1 using propidium iodide (dead cells) and Calcein-AM (live cells). (D) Quantification of the dead-cell ratio based on live/dead staining. (E) Mitochondrial membrane potential of H 2 O 2 -treated mBMSCs with or without IPA treatment for 1 day, assessed <t>by</t> <t>JC-1</t> staining. (F) Quantification of the red/green fluorescence ratio from JC-1 staining. All experiments were repeated at least three times. Data are presented as the mean ± SD. ** P<0.01 compared with the control group. IPA, indole-3-propionic acid; mBMSCs, mouse bone marrow stromal cells; CCK-8, Cell Counting Kit-8; ns, not significant.
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    IPA protects mBMSCs against H 2 O 2 -induced apoptotic injury. (A) Effects of IPA on mBMSC viability over 1, 2, and 3 days, as determined by the CCK-8 assay. (B) Effects of IPA pretreatment on mBMSC viability following H 2 O 2 exposure for 12, 24, and 48 h, as determined by the CCK-8 assay. (C) Live/dead staining of mBMSCs on day 1 using propidium iodide (dead cells) and Calcein-AM (live cells). (D) Quantification of the dead-cell ratio based on live/dead staining. (E) Mitochondrial membrane potential of H 2 O 2 -treated mBMSCs with or without IPA treatment for 1 day, assessed <t>by</t> <t>JC-1</t> staining. (F) Quantification of the red/green fluorescence ratio from JC-1 staining. All experiments were repeated at least three times. Data are presented as the mean ± SD. ** P<0.01 compared with the control group. IPA, indole-3-propionic acid; mBMSCs, mouse bone marrow stromal cells; CCK-8, Cell Counting Kit-8; ns, not significant.
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    PDK1 inhibitor JX06 and gefitinib synergistically induced cell apoptosis in gefitinib-resistant lung cancer cells. (A) The protein expression levels of PDK were reduced upon the treatment of PDK1 inhibitor JX06 in PC-9 and PC-9/G cells. (B) B2B and PC-9/G cells were treated with different concentrations of JX06 for 48 h. The cell viabilities were determined by CCK-8. (C – E) The synergy effect between JX06 and gefitinib was determined and analyzed with CompuSyn software. (F) The apoptosis rates were analyzed with flow cytometry after the combined treatment of gefitinib and JX06. (G) The TUNEL <t>assay</t> was performed with the indicated treatment in PC-9/G cells. (H) The cells treated as described were stained with the JC-1 probe and detected using a fluorescence microscope. Red fluorescence indicates the aggregation form of JC-1, showing increased mitochondrial <t>membrane</t> <t>potential</t> (ΔΨm). Green fluorescence indicates the monomeric form of JC-1, which indicates reduced mitochondrial membrane potential (ΔΨm). Data were statistically analyzed with Student’s t -test, and values were shown as mean ± standard deviation. ∗ P < 0.05 and ∗∗ P < 0.01.
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    Image Search Results


    IPA protects mBMSCs against H 2 O 2 -induced apoptotic injury. (A) Effects of IPA on mBMSC viability over 1, 2, and 3 days, as determined by the CCK-8 assay. (B) Effects of IPA pretreatment on mBMSC viability following H 2 O 2 exposure for 12, 24, and 48 h, as determined by the CCK-8 assay. (C) Live/dead staining of mBMSCs on day 1 using propidium iodide (dead cells) and Calcein-AM (live cells). (D) Quantification of the dead-cell ratio based on live/dead staining. (E) Mitochondrial membrane potential of H 2 O 2 -treated mBMSCs with or without IPA treatment for 1 day, assessed by JC-1 staining. (F) Quantification of the red/green fluorescence ratio from JC-1 staining. All experiments were repeated at least three times. Data are presented as the mean ± SD. ** P<0.01 compared with the control group. IPA, indole-3-propionic acid; mBMSCs, mouse bone marrow stromal cells; CCK-8, Cell Counting Kit-8; ns, not significant.

    Journal: International Journal of Molecular Medicine

    Article Title: Microbiota-derived indole-3-propionic acid reprograms bone marrow stem cell fate via PPARγ suppression to rescue osteoporosis

    doi: 10.3892/ijmm.2026.5879

    Figure Lengend Snippet: IPA protects mBMSCs against H 2 O 2 -induced apoptotic injury. (A) Effects of IPA on mBMSC viability over 1, 2, and 3 days, as determined by the CCK-8 assay. (B) Effects of IPA pretreatment on mBMSC viability following H 2 O 2 exposure for 12, 24, and 48 h, as determined by the CCK-8 assay. (C) Live/dead staining of mBMSCs on day 1 using propidium iodide (dead cells) and Calcein-AM (live cells). (D) Quantification of the dead-cell ratio based on live/dead staining. (E) Mitochondrial membrane potential of H 2 O 2 -treated mBMSCs with or without IPA treatment for 1 day, assessed by JC-1 staining. (F) Quantification of the red/green fluorescence ratio from JC-1 staining. All experiments were repeated at least three times. Data are presented as the mean ± SD. ** P<0.01 compared with the control group. IPA, indole-3-propionic acid; mBMSCs, mouse bone marrow stromal cells; CCK-8, Cell Counting Kit-8; ns, not significant.

    Article Snippet: MMP was assessed using a JC-1 assay kit (MedChemExpress).

    Techniques: CCK-8 Assay, Staining, Membrane, Fluorescence, Control, Cell Counting

    PDK1 inhibitor JX06 and gefitinib synergistically induced cell apoptosis in gefitinib-resistant lung cancer cells. (A) The protein expression levels of PDK were reduced upon the treatment of PDK1 inhibitor JX06 in PC-9 and PC-9/G cells. (B) B2B and PC-9/G cells were treated with different concentrations of JX06 for 48 h. The cell viabilities were determined by CCK-8. (C – E) The synergy effect between JX06 and gefitinib was determined and analyzed with CompuSyn software. (F) The apoptosis rates were analyzed with flow cytometry after the combined treatment of gefitinib and JX06. (G) The TUNEL assay was performed with the indicated treatment in PC-9/G cells. (H) The cells treated as described were stained with the JC-1 probe and detected using a fluorescence microscope. Red fluorescence indicates the aggregation form of JC-1, showing increased mitochondrial membrane potential (ΔΨm). Green fluorescence indicates the monomeric form of JC-1, which indicates reduced mitochondrial membrane potential (ΔΨm). Data were statistically analyzed with Student’s t -test, and values were shown as mean ± standard deviation. ∗ P < 0.05 and ∗∗ P < 0.01.

    Journal: Genes & Diseases

    Article Title: PDK1 elevation was induced by epigenetic modifications of KDM3A and METTL16 to mediate TKI resistance and cancer development

    doi: 10.1016/j.gendis.2025.101947

    Figure Lengend Snippet: PDK1 inhibitor JX06 and gefitinib synergistically induced cell apoptosis in gefitinib-resistant lung cancer cells. (A) The protein expression levels of PDK were reduced upon the treatment of PDK1 inhibitor JX06 in PC-9 and PC-9/G cells. (B) B2B and PC-9/G cells were treated with different concentrations of JX06 for 48 h. The cell viabilities were determined by CCK-8. (C – E) The synergy effect between JX06 and gefitinib was determined and analyzed with CompuSyn software. (F) The apoptosis rates were analyzed with flow cytometry after the combined treatment of gefitinib and JX06. (G) The TUNEL assay was performed with the indicated treatment in PC-9/G cells. (H) The cells treated as described were stained with the JC-1 probe and detected using a fluorescence microscope. Red fluorescence indicates the aggregation form of JC-1, showing increased mitochondrial membrane potential (ΔΨm). Green fluorescence indicates the monomeric form of JC-1, which indicates reduced mitochondrial membrane potential (ΔΨm). Data were statistically analyzed with Student’s t -test, and values were shown as mean ± standard deviation. ∗ P < 0.05 and ∗∗ P < 0.01.

    Article Snippet: After different treatments for 48 h, the mitochondrial membrane potential of cells was detected with the enhanced mitochondrial membrane potential assay kit using JC-1 probe (Beyotime, Jiangsu, China), and the fluorescence was analyzed with a fluorescence microscope.

    Techniques: Expressing, CCK-8 Assay, Software, Flow Cytometry, TUNEL Assay, Staining, Fluorescence, Microscopy, Membrane, Standard Deviation