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jc 1 assay kit  (MedChemExpress)
99
MedChemExpress jc 1 assay kit
IPA protects mBMSCs against H 2 O 2 -induced apoptotic injury. (A) Effects of IPA on mBMSC viability over 1, 2, and 3 days, as determined by the CCK-8 assay. (B) Effects of IPA pretreatment on mBMSC viability following H 2 O 2 exposure for 12, 24, and 48 h, as determined by the CCK-8 assay. (C) Live/dead staining of mBMSCs on day 1 using propidium iodide (dead cells) and Calcein-AM (live cells). (D) Quantification of the dead-cell ratio based on live/dead staining. (E) Mitochondrial membrane potential of H 2 O 2 -treated mBMSCs with or without IPA treatment for 1 day, assessed <t>by</t> <t>JC-1</t> staining. (F) Quantification of the red/green fluorescence ratio from JC-1 staining. All experiments were repeated at least three times. Data are presented as the mean ± SD. ** P<0.01 compared with the control group. IPA, indole-3-propionic acid; mBMSCs, mouse bone marrow stromal cells; CCK-8, Cell Counting Kit-8; ns, not significant.
Jc 1 Assay Kit, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/jc+1+kit/JC-1/pmc13252943-68-5-8
Average 99 stars, based on 1 article reviews
jc 1 assay kit - by Bioz Stars, 2026-09
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jc 1 kit  (Beyotime)
99
Beyotime jc 1 kit
IPA protects mBMSCs against H 2 O 2 -induced apoptotic injury. (A) Effects of IPA on mBMSC viability over 1, 2, and 3 days, as determined by the CCK-8 assay. (B) Effects of IPA pretreatment on mBMSC viability following H 2 O 2 exposure for 12, 24, and 48 h, as determined by the CCK-8 assay. (C) Live/dead staining of mBMSCs on day 1 using propidium iodide (dead cells) and Calcein-AM (live cells). (D) Quantification of the dead-cell ratio based on live/dead staining. (E) Mitochondrial membrane potential of H 2 O 2 -treated mBMSCs with or without IPA treatment for 1 day, assessed <t>by</t> <t>JC-1</t> staining. (F) Quantification of the red/green fluorescence ratio from JC-1 staining. All experiments were repeated at least three times. Data are presented as the mean ± SD. ** P<0.01 compared with the control group. IPA, indole-3-propionic acid; mBMSCs, mouse bone marrow stromal cells; CCK-8, Cell Counting Kit-8; ns, not significant.
Jc 1 Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/jc+1+kit/JC-1/pmc13084373-92-7-10
Average 99 stars, based on 1 article reviews
jc 1 kit - by Bioz Stars, 2026-09
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jc-1 mitochondrial membrane potential assay kit  (MedChemExpress)
98
MedChemExpress jc-1 mitochondrial membrane potential assay kit
IPA protects mBMSCs against H 2 O 2 -induced apoptotic injury. (A) Effects of IPA on mBMSC viability over 1, 2, and 3 days, as determined by the CCK-8 assay. (B) Effects of IPA pretreatment on mBMSC viability following H 2 O 2 exposure for 12, 24, and 48 h, as determined by the CCK-8 assay. (C) Live/dead staining of mBMSCs on day 1 using propidium iodide (dead cells) and Calcein-AM (live cells). (D) Quantification of the dead-cell ratio based on live/dead staining. (E) Mitochondrial membrane potential of H 2 O 2 -treated mBMSCs with or without IPA treatment for 1 day, assessed <t>by</t> <t>JC-1</t> staining. (F) Quantification of the red/green fluorescence ratio from JC-1 staining. All experiments were repeated at least three times. Data are presented as the mean ± SD. ** P<0.01 compared with the control group. IPA, indole-3-propionic acid; mBMSCs, mouse bone marrow stromal cells; CCK-8, Cell Counting Kit-8; ns, not significant.
Jc 1 Mitochondrial Membrane Potential Assay Kit, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/jc+1+kit/JC-1+Mitochondrial+Membrane+Potential+Assay+Kit/custom%40hy-k0601%4042456829
Average 98 stars, based on 1 article reviews
jc-1 mitochondrial membrane potential assay kit - by Bioz Stars, 2026-09
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membrane potential assay kit  (Beyotime)
99
Beyotime membrane potential assay kit
PDK1 inhibitor JX06 and gefitinib synergistically induced cell apoptosis in gefitinib-resistant lung cancer cells. (A) The protein expression levels of PDK were reduced upon the treatment of PDK1 inhibitor JX06 in PC-9 and PC-9/G cells. (B) B2B and PC-9/G cells were treated with different concentrations of JX06 for 48 h. The cell viabilities were determined by CCK-8. (C – E) The synergy effect between JX06 and gefitinib was determined and analyzed with CompuSyn software. (F) The apoptosis rates were analyzed with flow cytometry after the combined treatment of gefitinib and JX06. (G) The TUNEL <t>assay</t> was performed with the indicated treatment in PC-9/G cells. (H) The cells treated as described were stained with the JC-1 probe and detected using a fluorescence microscope. Red fluorescence indicates the aggregation form of JC-1, showing increased mitochondrial <t>membrane</t> <t>potential</t> (ΔΨm). Green fluorescence indicates the monomeric form of JC-1, which indicates reduced mitochondrial membrane potential (ΔΨm). Data were statistically analyzed with Student’s t -test, and values were shown as mean ± standard deviation. ∗ P < 0.05 and ∗∗ P < 0.01.
Membrane Potential Assay Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/jc+1+kit/Enhanced+mitochondrial+membrane+potential+assay+kit+with+JC-1/pmc13091346-69-18-25
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membrane potential assay kit - by Bioz Stars, 2026-09
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nigericin  (MedChemExpress)
98
MedChemExpress nigericin
PDK1 inhibitor JX06 and gefitinib synergistically induced cell apoptosis in gefitinib-resistant lung cancer cells. (A) The protein expression levels of PDK were reduced upon the treatment of PDK1 inhibitor JX06 in PC-9 and PC-9/G cells. (B) B2B and PC-9/G cells were treated with different concentrations of JX06 for 48 h. The cell viabilities were determined by CCK-8. (C – E) The synergy effect between JX06 and gefitinib was determined and analyzed with CompuSyn software. (F) The apoptosis rates were analyzed with flow cytometry after the combined treatment of gefitinib and JX06. (G) The TUNEL <t>assay</t> was performed with the indicated treatment in PC-9/G cells. (H) The cells treated as described were stained with the JC-1 probe and detected using a fluorescence microscope. Red fluorescence indicates the aggregation form of JC-1, showing increased mitochondrial <t>membrane</t> <t>potential</t> (ΔΨm). Green fluorescence indicates the monomeric form of JC-1, which indicates reduced mitochondrial membrane potential (ΔΨm). Data were statistically analyzed with Student’s t -test, and values were shown as mean ± standard deviation. ∗ P < 0.05 and ∗∗ P < 0.01.
Nigericin, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/jc+1+kit/JC-1+Mitochondrial+Membrane+Potential+Assay+Kit/pm42364560-54-2-11
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nigericin - by Bioz Stars, 2026-09
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jc 1 assay kit  (Keygen Biotech)
86
Keygen Biotech jc 1 assay kit
PDK1 inhibitor JX06 and gefitinib synergistically induced cell apoptosis in gefitinib-resistant lung cancer cells. (A) The protein expression levels of PDK were reduced upon the treatment of PDK1 inhibitor JX06 in PC-9 and PC-9/G cells. (B) B2B and PC-9/G cells were treated with different concentrations of JX06 for 48 h. The cell viabilities were determined by CCK-8. (C – E) The synergy effect between JX06 and gefitinib was determined and analyzed with CompuSyn software. (F) The apoptosis rates were analyzed with flow cytometry after the combined treatment of gefitinib and JX06. (G) The TUNEL <t>assay</t> was performed with the indicated treatment in PC-9/G cells. (H) The cells treated as described were stained with the JC-1 probe and detected using a fluorescence microscope. Red fluorescence indicates the aggregation form of JC-1, showing increased mitochondrial <t>membrane</t> <t>potential</t> (ΔΨm). Green fluorescence indicates the monomeric form of JC-1, which indicates reduced mitochondrial membrane potential (ΔΨm). Data were statistically analyzed with Student’s t -test, and values were shown as mean ± standard deviation. ∗ P < 0.05 and ∗∗ P < 0.01.
Jc 1 Assay Kit, supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/jc+1+kit/1+apoptosis+detection+jc+kit/pm42320118-116-6-9
Average 86 stars, based on 1 article reviews
jc 1 assay kit - by Bioz Stars, 2026-09
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mitochondrial membrane potential  (MedChemExpress)
98
MedChemExpress mitochondrial membrane potential
PDK1 inhibitor JX06 and gefitinib synergistically induced cell apoptosis in gefitinib-resistant lung cancer cells. (A) The protein expression levels of PDK were reduced upon the treatment of PDK1 inhibitor JX06 in PC-9 and PC-9/G cells. (B) B2B and PC-9/G cells were treated with different concentrations of JX06 for 48 h. The cell viabilities were determined by CCK-8. (C – E) The synergy effect between JX06 and gefitinib was determined and analyzed with CompuSyn software. (F) The apoptosis rates were analyzed with flow cytometry after the combined treatment of gefitinib and JX06. (G) The TUNEL <t>assay</t> was performed with the indicated treatment in PC-9/G cells. (H) The cells treated as described were stained with the JC-1 probe and detected using a fluorescence microscope. Red fluorescence indicates the aggregation form of JC-1, showing increased mitochondrial <t>membrane</t> <t>potential</t> (ΔΨm). Green fluorescence indicates the monomeric form of JC-1, which indicates reduced mitochondrial membrane potential (ΔΨm). Data were statistically analyzed with Student’s t -test, and values were shown as mean ± standard deviation. ∗ P < 0.05 and ∗∗ P < 0.01.
Mitochondrial Membrane Potential, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/jc+1+kit/JC-1+Mitochondrial+Membrane+Potential+Assay+Kit/pm42281376-121-0-8
Average 98 stars, based on 1 article reviews
mitochondrial membrane potential - by Bioz Stars, 2026-09
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fluorescent probe  (MedChemExpress)
98
MedChemExpress fluorescent probe
PDK1 inhibitor JX06 and gefitinib synergistically induced cell apoptosis in gefitinib-resistant lung cancer cells. (A) The protein expression levels of PDK were reduced upon the treatment of PDK1 inhibitor JX06 in PC-9 and PC-9/G cells. (B) B2B and PC-9/G cells were treated with different concentrations of JX06 for 48 h. The cell viabilities were determined by CCK-8. (C – E) The synergy effect between JX06 and gefitinib was determined and analyzed with CompuSyn software. (F) The apoptosis rates were analyzed with flow cytometry after the combined treatment of gefitinib and JX06. (G) The TUNEL <t>assay</t> was performed with the indicated treatment in PC-9/G cells. (H) The cells treated as described were stained with the JC-1 probe and detected using a fluorescence microscope. Red fluorescence indicates the aggregation form of JC-1, showing increased mitochondrial <t>membrane</t> <t>potential</t> (ΔΨm). Green fluorescence indicates the monomeric form of JC-1, which indicates reduced mitochondrial membrane potential (ΔΨm). Data were statistically analyzed with Student’s t -test, and values were shown as mean ± standard deviation. ∗ P < 0.05 and ∗∗ P < 0.01.
Fluorescent Probe, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/jc+1+kit/JC-1+Mitochondrial+Membrane+Potential+Assay+Kit/pm42235794-167-2-4
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fluorescent probe - by Bioz Stars, 2026-09
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IPA protects mBMSCs against H 2 O 2 -induced apoptotic injury. (A) Effects of IPA on mBMSC viability over 1, 2, and 3 days, as determined by the CCK-8 assay. (B) Effects of IPA pretreatment on mBMSC viability following H 2 O 2 exposure for 12, 24, and 48 h, as determined by the CCK-8 assay. (C) Live/dead staining of mBMSCs on day 1 using propidium iodide (dead cells) and Calcein-AM (live cells). (D) Quantification of the dead-cell ratio based on live/dead staining. (E) Mitochondrial membrane potential of H 2 O 2 -treated mBMSCs with or without IPA treatment for 1 day, assessed by JC-1 staining. (F) Quantification of the red/green fluorescence ratio from JC-1 staining. All experiments were repeated at least three times. Data are presented as the mean ± SD. ** P<0.01 compared with the control group. IPA, indole-3-propionic acid; mBMSCs, mouse bone marrow stromal cells; CCK-8, Cell Counting Kit-8; ns, not significant.

Journal: International Journal of Molecular Medicine

Article Title: Microbiota-derived indole-3-propionic acid reprograms bone marrow stem cell fate via PPARγ suppression to rescue osteoporosis

doi: 10.3892/ijmm.2026.5879

Figure Lengend Snippet: IPA protects mBMSCs against H 2 O 2 -induced apoptotic injury. (A) Effects of IPA on mBMSC viability over 1, 2, and 3 days, as determined by the CCK-8 assay. (B) Effects of IPA pretreatment on mBMSC viability following H 2 O 2 exposure for 12, 24, and 48 h, as determined by the CCK-8 assay. (C) Live/dead staining of mBMSCs on day 1 using propidium iodide (dead cells) and Calcein-AM (live cells). (D) Quantification of the dead-cell ratio based on live/dead staining. (E) Mitochondrial membrane potential of H 2 O 2 -treated mBMSCs with or without IPA treatment for 1 day, assessed by JC-1 staining. (F) Quantification of the red/green fluorescence ratio from JC-1 staining. All experiments were repeated at least three times. Data are presented as the mean ± SD. ** P<0.01 compared with the control group. IPA, indole-3-propionic acid; mBMSCs, mouse bone marrow stromal cells; CCK-8, Cell Counting Kit-8; ns, not significant.

Article Snippet: MMP was assessed using a JC-1 assay kit (MedChemExpress).

Techniques: CCK-8 Assay, Staining, Membrane, Fluorescence, Control, Cell Counting

PDK1 inhibitor JX06 and gefitinib synergistically induced cell apoptosis in gefitinib-resistant lung cancer cells. (A) The protein expression levels of PDK were reduced upon the treatment of PDK1 inhibitor JX06 in PC-9 and PC-9/G cells. (B) B2B and PC-9/G cells were treated with different concentrations of JX06 for 48 h. The cell viabilities were determined by CCK-8. (C – E) The synergy effect between JX06 and gefitinib was determined and analyzed with CompuSyn software. (F) The apoptosis rates were analyzed with flow cytometry after the combined treatment of gefitinib and JX06. (G) The TUNEL assay was performed with the indicated treatment in PC-9/G cells. (H) The cells treated as described were stained with the JC-1 probe and detected using a fluorescence microscope. Red fluorescence indicates the aggregation form of JC-1, showing increased mitochondrial membrane potential (ΔΨm). Green fluorescence indicates the monomeric form of JC-1, which indicates reduced mitochondrial membrane potential (ΔΨm). Data were statistically analyzed with Student’s t -test, and values were shown as mean ± standard deviation. ∗ P < 0.05 and ∗∗ P < 0.01.

Journal: Genes & Diseases

Article Title: PDK1 elevation was induced by epigenetic modifications of KDM3A and METTL16 to mediate TKI resistance and cancer development

doi: 10.1016/j.gendis.2025.101947

Figure Lengend Snippet: PDK1 inhibitor JX06 and gefitinib synergistically induced cell apoptosis in gefitinib-resistant lung cancer cells. (A) The protein expression levels of PDK were reduced upon the treatment of PDK1 inhibitor JX06 in PC-9 and PC-9/G cells. (B) B2B and PC-9/G cells were treated with different concentrations of JX06 for 48 h. The cell viabilities were determined by CCK-8. (C – E) The synergy effect between JX06 and gefitinib was determined and analyzed with CompuSyn software. (F) The apoptosis rates were analyzed with flow cytometry after the combined treatment of gefitinib and JX06. (G) The TUNEL assay was performed with the indicated treatment in PC-9/G cells. (H) The cells treated as described were stained with the JC-1 probe and detected using a fluorescence microscope. Red fluorescence indicates the aggregation form of JC-1, showing increased mitochondrial membrane potential (ΔΨm). Green fluorescence indicates the monomeric form of JC-1, which indicates reduced mitochondrial membrane potential (ΔΨm). Data were statistically analyzed with Student’s t -test, and values were shown as mean ± standard deviation. ∗ P < 0.05 and ∗∗ P < 0.01.

Article Snippet: After different treatments for 48 h, the mitochondrial membrane potential of cells was detected with the enhanced mitochondrial membrane potential assay kit using JC-1 probe (Beyotime, Jiangsu, China), and the fluorescence was analyzed with a fluorescence microscope.

Techniques: Expressing, CCK-8 Assay, Software, Flow Cytometry, TUNEL Assay, Staining, Fluorescence, Microscopy, Membrane, Standard Deviation