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Proteintech anti jak1
Anti Jak1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 175 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/jak1/JAK1+Antibody/pm41933939-328-55-56
Average 96 stars, based on 175 article reviews
anti jak1 - by Bioz Stars, 2026-09
96/100 stars

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Related Articles

Membrane:

Article Title: Integrative multi-omics analysis proposes a metabolic classification of gliomas: distinct metabolic states, immune infiltration, and prognosis.
Article Snippet: Proteins were separated by gel electrophoresis and transferred onto a polyvinylidene fluoride (PVDF) membrane (Merck, Germany). .. The membrane was blocked in 5% non-fat milk for 1 hour and incubated with primary antibodies against OSMR (10982-1-AP, Proteintech), HK-2(22029-1-AP, Proteintech), LDHA (sc-137244, Santa Cruz), JAK1 (66466-1-lg, Proteintech), p-JAK1 (ab138005, abcam), STAT3 (ab68152, abcam), p-STAT3 (ab267373, abcam), β-actin (66009-1-Ig, Proteintech) overnight at 4°C. .. Following three washes with 1×TBST, the PVDF membrane was incubated with a horseradish peroxidase (HRP)-conjugated secondary antibody (Sangon Biotech, China) at room temperature for 1 hour.

Article Title: Integrative multi-omics analysis proposes a metabolic classification of gliomas: distinct metabolic states, immune infiltration, and prognosis
Article Snippet: Proteins were separated by gel electrophoresis and transferred onto a polyvinylidene fluoride (PVDF) membrane (Merck, Germany). .. The membrane was blocked in 5% non-fat milk for 1 hour and incubated with primary antibodies against OSMR (10982-1-AP, Proteintech), HK-2(22029-1-AP, Proteintech), LDHA (sc-137244, Santa Cruz), JAK1 (66466-1-lg, Proteintech), p-JAK1 (ab138005, abcam), STAT3 (ab68152, abcam), p-STAT3 (ab267373, abcam), β-actin (66009-1-Ig, Proteintech) overnight at 4 °C. .. Following three washes with 1×TBST, the PVDF membrane was incubated with a horseradish peroxidase (HRP)-conjugated secondary antibody (Sangon Biotech, China) at room temperature for 1 hour.

Incubation:

Article Title: Integrative multi-omics analysis proposes a metabolic classification of gliomas: distinct metabolic states, immune infiltration, and prognosis.
Article Snippet: Proteins were separated by gel electrophoresis and transferred onto a polyvinylidene fluoride (PVDF) membrane (Merck, Germany). .. The membrane was blocked in 5% non-fat milk for 1 hour and incubated with primary antibodies against OSMR (10982-1-AP, Proteintech), HK-2(22029-1-AP, Proteintech), LDHA (sc-137244, Santa Cruz), JAK1 (66466-1-lg, Proteintech), p-JAK1 (ab138005, abcam), STAT3 (ab68152, abcam), p-STAT3 (ab267373, abcam), β-actin (66009-1-Ig, Proteintech) overnight at 4°C. .. Following three washes with 1×TBST, the PVDF membrane was incubated with a horseradish peroxidase (HRP)-conjugated secondary antibody (Sangon Biotech, China) at room temperature for 1 hour.

Article Title: Revealing an unconventional JAK1/2-STAT3 branch in macrophage IFN-γ signaling.
Article Snippet: Protein samples (10 μg each) were prepared using BOLT SDS/LDS Sample Buffer (4×, Novex) and Sample Reducing Agent (10×, Novex), heated at 70◦C for 10 min, and loaded onto pre-cast BOLT Novex 4–12% Bis-Tris gels (10- or 15-well, Invitrogen) with 1× BOLT MOPS SDS Running Buffer. .. Protein was transferred to Trans-Blot Turbo RTA Mini 0.2 μm Nitrocellulose membranes (Bio-Rad) using the Trans-Blot Turbo system (Bio-Rad) after which the membranes were blocked overnight at 4◦C in TBS with 5% BSA and incubated overnight at 4◦C with primary antibodies: α-tubulin (Proteintech), Phospho-STAT1 (Invitrogen), STAT1 (Cell Signaling), PhosphoSTAT3 (Cell Signaling), STAT3 (Cell Signaling), JAK1 (ProteinTech), JAK2 (Cell Signaling), JAK3 (Cell Signaling) and TYK2 (ProteinTech) all diluted 1:1000 in TBS-1% BSA, except α-tubulin (1:5000). .. Goat Anti-Mouse and Goat Anti-Rabbit Secondary antibodies (1:5000, Bio-Rad) were applied for 1 h at room temperature.

Article Title: Luteolin ameliorates steroid-induced osteonecrosis of the femoral head via a gut microbiota–L-Carnitine–IFIH1 axis
Article Snippet: Cells were lysed in ice-cold RIPA buffer (Beyotime, China; cat. no. P0013B) supplemented with phosphatase inhibitors for 30 min. Lysates were centrifuged at 12,000 rpm for 10 min at 4 °C, and protein concentrations were determined by BCA assay (Beyotime, China; cat. no. P0010). .. Equal protein amounts (20 μg) were separated on 10% SDS-PAGE and transferred to PVDF membranes (Millipore, USA; cat. no. IPVH00010) using wet transfer at 100 V for 70 min. Membranes were blocked with 5% non-fat milk/TBST for 1 h at 25 °C, then incubated overnight at 4 °C with primary antibodies against: JAK1 (Proteintech, China; cat. no. 66466-1-Ig; 1:6000), STAT1 (Proteintech, China; cat. no. 10144-2-AP; 1:6000), IRF1 (Proteintech, China; cat. no. 11335-1-AP; 1:1000), OAS1 (FineTest, China; cat. no. FNab10795; 1:1000), IFI44 (Bioswamp, China; cat. no. PAB35743 ; 1:1000), IFIH1 (Bioswamp, China; cat. no. PAB31693 ; 1:1000) and GAPDH (Bioss, China; cat. no. bs-2188R; 1:6000). .. After 3 × 5-min TBST washes, membranes were incubated with HRP-conjugated secondary antibody (Abcam, UK; cat. no. ab205718; 1:10,000) for 1.5 h at 25 °C.

Article Title: Quercetin Targets IL6/JAK1/STAT3/MMP9 Signaling to Attenuate Breast Cancer Progression in Diabetic Comorbidity: A Multi-omics and Experimental Study
Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

Article Title: Integrative multi-omics analysis proposes a metabolic classification of gliomas: distinct metabolic states, immune infiltration, and prognosis
Article Snippet: Proteins were separated by gel electrophoresis and transferred onto a polyvinylidene fluoride (PVDF) membrane (Merck, Germany). .. The membrane was blocked in 5% non-fat milk for 1 hour and incubated with primary antibodies against OSMR (10982-1-AP, Proteintech), HK-2(22029-1-AP, Proteintech), LDHA (sc-137244, Santa Cruz), JAK1 (66466-1-lg, Proteintech), p-JAK1 (ab138005, abcam), STAT3 (ab68152, abcam), p-STAT3 (ab267373, abcam), β-actin (66009-1-Ig, Proteintech) overnight at 4 °C. .. Following three washes with 1×TBST, the PVDF membrane was incubated with a horseradish peroxidase (HRP)-conjugated secondary antibody (Sangon Biotech, China) at room temperature for 1 hour.

Article Title: Zhi Chuan Ling oral liquid alleviates Th2-type asthma via IL-4-JAK1-STAT6 and KIT signaling pathways.
Article Snippet: Ethnopharmacological relevance: Zhi Chuan Ling Oral Liquid (ZCL) has long been used in traditional Chinese medicine for the treatment of asthma.. However, how ZCL acts on Th2-type asthma and its active components

SDS Page:

Article Title: Luteolin ameliorates steroid-induced osteonecrosis of the femoral head via a gut microbiota–L-Carnitine–IFIH1 axis
Article Snippet: Cells were lysed in ice-cold RIPA buffer (Beyotime, China; cat. no. P0013B) supplemented with phosphatase inhibitors for 30 min. Lysates were centrifuged at 12,000 rpm for 10 min at 4 °C, and protein concentrations were determined by BCA assay (Beyotime, China; cat. no. P0010). .. Equal protein amounts (20 μg) were separated on 10% SDS-PAGE and transferred to PVDF membranes (Millipore, USA; cat. no. IPVH00010) using wet transfer at 100 V for 70 min. Membranes were blocked with 5% non-fat milk/TBST for 1 h at 25 °C, then incubated overnight at 4 °C with primary antibodies against: JAK1 (Proteintech, China; cat. no. 66466-1-Ig; 1:6000), STAT1 (Proteintech, China; cat. no. 10144-2-AP; 1:6000), IRF1 (Proteintech, China; cat. no. 11335-1-AP; 1:1000), OAS1 (FineTest, China; cat. no. FNab10795; 1:1000), IFI44 (Bioswamp, China; cat. no. PAB35743 ; 1:1000), IFIH1 (Bioswamp, China; cat. no. PAB31693 ; 1:1000) and GAPDH (Bioss, China; cat. no. bs-2188R; 1:6000). .. After 3 × 5-min TBST washes, membranes were incubated with HRP-conjugated secondary antibody (Abcam, UK; cat. no. ab205718; 1:10,000) for 1.5 h at 25 °C.

MTT Assay:

Article Title: Pleurotus citrinopileatus polysaccharide inhibits glioma growth by downregulating the expression of CHI3L1 CHI3L1 as a therapeutic target in glioma
Article Snippet: Pleurotus citrinopileatus polysaccharide (PCFP) demonstrates anti-glioma potential.. We found that PCFP significantly inhibited the survival rate of glioma cells as determined by MTT assay.. Additionally, 100 mg/kg PCFP significantly inhibited the growth of U251 tumors in nude mice xenograft models, with a tumor inhibition rate of 61.41%; no significant toxicity was observed in organs such as the liver, spleen, or heart.

Bioprocessing:

Article Title: Pleurotus citrinopileatus polysaccharide inhibits glioma growth by downregulating the expression of CHI3L1 CHI3L1 as a therapeutic target in glioma
Article Snippet: Pleurotus citrinopileatus polysaccharide (PCFP) demonstrates anti-glioma potential.. We found that PCFP significantly inhibited the survival rate of glioma cells as determined by MTT assay.. Additionally, 100 mg/kg PCFP significantly inhibited the growth of U251 tumors in nude mice xenograft models, with a tumor inhibition rate of 61.41%; no significant toxicity was observed in organs such as the liver, spleen, or heart.

Blocking Assay:

Article Title: Quercetin Targets IL6/JAK1/STAT3/MMP9 Signaling to Attenuate Breast Cancer Progression in Diabetic Comorbidity: A Multi-omics and Experimental Study
Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

Article Title: Zhi Chuan Ling oral liquid alleviates Th2-type asthma via IL-4-JAK1-STAT6 and KIT signaling pathways.
Article Snippet: Ethnopharmacological relevance: Zhi Chuan Ling Oral Liquid (ZCL) has long been used in traditional Chinese medicine for the treatment of asthma.. However, how ZCL acts on Th2-type asthma and its active components



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