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anti iκbα  (Proteintech)


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    Structured Review

    Proteintech anti iκbα
    Anti Iκbα, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 427 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/i%CE%BAb%CE%B1/IkB+alpha+Polyclonal+antibody/pmc13019499-250-26-29
    Average 96 stars, based on 427 article reviews
    anti iκbα - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    other:

    Article Title: ATF6 ameliorates renal warm ischemia-reperfusion injury through FHL2-mediated NF-κB signaling pathway
    Article Snippet: IκBα , Proteintech , Cat# 10268-1-AP; RRID: AB_2151423.

    Article Title: TFEB degradation is regulated by an IKK/β-TrCP2 phosphorylation-ubiquitination cascade.
    Article Snippet: For viral transduction, HeLa cells and MEFs with C57BL/6 background (Dr. Mark Sands’ laboratory, Washington University in St. Louis) were seeded at 50–60% confluence and treated overnight with the medium containing freshly harvested lentivirus in the presence of 8 μg/mL polybrene (Cat# TRAR TI CL E IN P RE SS ARTICLE IN PRESS 1003-G, MilliporeSigma).

    Blocking Assay:

    Article Title: Cepharanthine alleviated chronic prostatitis/chronic pelvic pain syndrome by activating Nrf2 and inhibiting the NF-κB pathway
    Article Snippet: Protein concentrations were quantified, and equal amounts were separated by SDS-PAGE before transferring to PVDF membranes (Millipore, USA). .. After blocking with 5% skim milk for 2 h, membranes were incubated with primary antibodies, COX-2 (1:2000, 12375-1-AP, Proteintech), iNOS (1:1000, 22226-1-AP, Proteintech), IL-1β (1:1000, 16806-1-AP, Proteintech), IL-6 (1:1000, 21865-1-AP, Proteintech), CCL2 (1:1000, ab214819, Abcam), Nrf2 (1:10000, 16396-1-AP, Proteintech), HO-1 (1:3000, 10701-1-AP, Proteintech), NQO1 (1:4000, 11451-1-AP, Proteintech), P65 (1:1000, 10745-1-AP, Proteintech), P-P65 (1:5000, 82335-1-RR, Proteintech), IκBα (1:10000, 10268-1-AP, Proteintech), P-IκBα (1:2000, 82349-1-RR, Proteintech), Bax (1:10000, 50599-1-Ig, Proteintech), Bcl-2 (1:1000, 12789-1-AP, Proteintech), caspase-3 (1:500, 19677-1-AP, Proteintech), Lamin B (1:2000, 129871-AP, Proteintech), β-Tubulin (1:5000, 10094-1-AP, Proteintech), and GAPDH (1:50000, 60004-1-Ig, Proteintech), protein bands were visualized using chemiluminescent HRP substrate (Millipore, USA) and detected with the Fusion FX imaging system (Vilber Lourmat, France). ..

    Article Title: Royal jelly protein hydrolysates accelerate in vitro diabetic wound healing by enhancing keratinocytes functions and suppressing macrophage-mediated inflammation.
    Article Snippet: The FuturePAGETM gel (10%, ACE, China) was used for protein separation and the protein was transferred onto polyvinylidene fluoride (PVDF) membranes (0.45μm, Millipore, USA). .. The membranes were incubated with primary antibodies anti-INOS (1 : 1000, 18985-1-AP, proteintech, China), COX-2 (1 : 1000, 66351-1-Ig, proteintech, China), IKKα (1 : 1000, bs-2907R, Bioss, China), phospho-IKKα (p-IKKα) (1 : 1000, AP0505, Abclonal, China), IκBα (1 : 1000, 10268-1-AP, proteintech, China), phospho-IκBα (p-IκBα) (1 : 1000, 82349-1-RR, proteintech, China), p65 (1 : 1000, 380172, ZENBIO, China), phospho-p65 (pAR TIC LE IN PR ES S p65) (1 : 1000, 82335-1-RR, proteintech, China), β-Actin (1 : 10000, 66009-1-Ig, proteintech, China) and β-Tublin (1 : 5000, 10094-1-AP, proteintech, China) at 4°C overnight after blocked with Fish Gelatin Fast Blocking Kit (ZENBIO, China) and then incubated with secondary antibodies HRPconjugated Affinipure Goat Anti-Rabbit IgG(H+L) (1:20000, SA00001-2, proteintech, China) or HRP-conjugated Affinipure Goat Anti-Mouse IgG(H+L) (1:20000, SA00001-1, proteintech, China)for 45 min at room temperature. .. Images of blotting were acquired using Omni-ECLTM Pico Light Chemiluminescence Kit (SQ202, Epizyme, China) and analyzed by Image J.

    Incubation:

    Article Title: Cepharanthine alleviated chronic prostatitis/chronic pelvic pain syndrome by activating Nrf2 and inhibiting the NF-κB pathway
    Article Snippet: Protein concentrations were quantified, and equal amounts were separated by SDS-PAGE before transferring to PVDF membranes (Millipore, USA). .. After blocking with 5% skim milk for 2 h, membranes were incubated with primary antibodies, COX-2 (1:2000, 12375-1-AP, Proteintech), iNOS (1:1000, 22226-1-AP, Proteintech), IL-1β (1:1000, 16806-1-AP, Proteintech), IL-6 (1:1000, 21865-1-AP, Proteintech), CCL2 (1:1000, ab214819, Abcam), Nrf2 (1:10000, 16396-1-AP, Proteintech), HO-1 (1:3000, 10701-1-AP, Proteintech), NQO1 (1:4000, 11451-1-AP, Proteintech), P65 (1:1000, 10745-1-AP, Proteintech), P-P65 (1:5000, 82335-1-RR, Proteintech), IκBα (1:10000, 10268-1-AP, Proteintech), P-IκBα (1:2000, 82349-1-RR, Proteintech), Bax (1:10000, 50599-1-Ig, Proteintech), Bcl-2 (1:1000, 12789-1-AP, Proteintech), caspase-3 (1:500, 19677-1-AP, Proteintech), Lamin B (1:2000, 129871-AP, Proteintech), β-Tubulin (1:5000, 10094-1-AP, Proteintech), and GAPDH (1:50000, 60004-1-Ig, Proteintech), protein bands were visualized using chemiluminescent HRP substrate (Millipore, USA) and detected with the Fusion FX imaging system (Vilber Lourmat, France). ..

    Article Title: Royal jelly protein hydrolysates accelerate in vitro diabetic wound healing by enhancing keratinocytes functions and suppressing macrophage-mediated inflammation.
    Article Snippet: The FuturePAGETM gel (10%, ACE, China) was used for protein separation and the protein was transferred onto polyvinylidene fluoride (PVDF) membranes (0.45μm, Millipore, USA). .. The membranes were incubated with primary antibodies anti-INOS (1 : 1000, 18985-1-AP, proteintech, China), COX-2 (1 : 1000, 66351-1-Ig, proteintech, China), IKKα (1 : 1000, bs-2907R, Bioss, China), phospho-IKKα (p-IKKα) (1 : 1000, AP0505, Abclonal, China), IκBα (1 : 1000, 10268-1-AP, proteintech, China), phospho-IκBα (p-IκBα) (1 : 1000, 82349-1-RR, proteintech, China), p65 (1 : 1000, 380172, ZENBIO, China), phospho-p65 (pAR TIC LE IN PR ES S p65) (1 : 1000, 82335-1-RR, proteintech, China), β-Actin (1 : 10000, 66009-1-Ig, proteintech, China) and β-Tublin (1 : 5000, 10094-1-AP, proteintech, China) at 4°C overnight after blocked with Fish Gelatin Fast Blocking Kit (ZENBIO, China) and then incubated with secondary antibodies HRPconjugated Affinipure Goat Anti-Rabbit IgG(H+L) (1:20000, SA00001-2, proteintech, China) or HRP-conjugated Affinipure Goat Anti-Mouse IgG(H+L) (1:20000, SA00001-1, proteintech, China)for 45 min at room temperature. .. Images of blotting were acquired using Omni-ECLTM Pico Light Chemiluminescence Kit (SQ202, Epizyme, China) and analyzed by Image J.

    Imaging:

    Article Title: Cepharanthine alleviated chronic prostatitis/chronic pelvic pain syndrome by activating Nrf2 and inhibiting the NF-κB pathway
    Article Snippet: Protein concentrations were quantified, and equal amounts were separated by SDS-PAGE before transferring to PVDF membranes (Millipore, USA). .. After blocking with 5% skim milk for 2 h, membranes were incubated with primary antibodies, COX-2 (1:2000, 12375-1-AP, Proteintech), iNOS (1:1000, 22226-1-AP, Proteintech), IL-1β (1:1000, 16806-1-AP, Proteintech), IL-6 (1:1000, 21865-1-AP, Proteintech), CCL2 (1:1000, ab214819, Abcam), Nrf2 (1:10000, 16396-1-AP, Proteintech), HO-1 (1:3000, 10701-1-AP, Proteintech), NQO1 (1:4000, 11451-1-AP, Proteintech), P65 (1:1000, 10745-1-AP, Proteintech), P-P65 (1:5000, 82335-1-RR, Proteintech), IκBα (1:10000, 10268-1-AP, Proteintech), P-IκBα (1:2000, 82349-1-RR, Proteintech), Bax (1:10000, 50599-1-Ig, Proteintech), Bcl-2 (1:1000, 12789-1-AP, Proteintech), caspase-3 (1:500, 19677-1-AP, Proteintech), Lamin B (1:2000, 129871-AP, Proteintech), β-Tubulin (1:5000, 10094-1-AP, Proteintech), and GAPDH (1:50000, 60004-1-Ig, Proteintech), protein bands were visualized using chemiluminescent HRP substrate (Millipore, USA) and detected with the Fusion FX imaging system (Vilber Lourmat, France). ..



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