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iwp 2  (MedChemExpress)


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    MedChemExpress iwp 2
    Blockade of Wnt/β-catenin signaling partially abolished the improvement effects of gastrodin on neurogenesis and behaviors in stress-exposed mice (A-B) Scheme for blocking the Wnt/β-catenin signaling <t>with</t> <t>IWP-2</t> or ICG-001 in stress-exposed mice in present of Gastrodin (GAS) treatment. SPT, sucrose preference test; OFT, open field test; EPMT, elevated plus maze test; FST, forced swimming test. (C – H) Effects of Wnt/β-catenin signaling blockade by IWP-2 or ICG-001 by IWP-2 or ICG-001 on levels of Wnt, β-catenin, p -β-catenin, C-Myc and Cyclin D1 in hippocampus of GAS + Stress mice. (I-N) Effects of Wnt/β-catenin signaling blockade by IWP-2 or ICG-001 on the hippocampal neurogenesis of stress-exposed mice treated with GAS. Proliferating NSPCs were labeled with BrdU (green), and immature neurons were labeled with Doublecortin (DCX), mature neurons were labeled with NeuN, the newborn immature neurons differentiated from NSPCs were labeled both with DCX and BrdU, the newborn mature neurons were labeled with both BrdU and NeuN, and their number was quantified. (O-R) Effects of Wnt/β-catenin signaling blockade by IWP-2 or ICG-001 on the enhanced stress resilience effects of GAS (n = 8). Data are mean ± standard error of the mean (SEM). * p < 0.05, ** p < 0.01, *** p < 0.001 vs Control group, # p < 0.05, ### p < 0.001 vs Stress group, & p < 0.05, && p < 0.01, && p < 0.01 vs GAS group (one-way ANOVA with Tukey's multiple-comparisons test). Details of the statistical analyses are provided in Supplementary Table 7. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Iwp 2, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Gastrodin enhances stress resilience through promoting Wnt/β-Catenin-dependent neurogenesis"

    Article Title: Gastrodin enhances stress resilience through promoting Wnt/β-Catenin-dependent neurogenesis

    Journal: Journal of Advanced Research

    doi: 10.1016/j.jare.2025.04.017

    Blockade of Wnt/β-catenin signaling partially abolished the improvement effects of gastrodin on neurogenesis and behaviors in stress-exposed mice (A-B) Scheme for blocking the Wnt/β-catenin signaling with IWP-2 or ICG-001 in stress-exposed mice in present of Gastrodin (GAS) treatment. SPT, sucrose preference test; OFT, open field test; EPMT, elevated plus maze test; FST, forced swimming test. (C – H) Effects of Wnt/β-catenin signaling blockade by IWP-2 or ICG-001 by IWP-2 or ICG-001 on levels of Wnt, β-catenin, p -β-catenin, C-Myc and Cyclin D1 in hippocampus of GAS + Stress mice. (I-N) Effects of Wnt/β-catenin signaling blockade by IWP-2 or ICG-001 on the hippocampal neurogenesis of stress-exposed mice treated with GAS. Proliferating NSPCs were labeled with BrdU (green), and immature neurons were labeled with Doublecortin (DCX), mature neurons were labeled with NeuN, the newborn immature neurons differentiated from NSPCs were labeled both with DCX and BrdU, the newborn mature neurons were labeled with both BrdU and NeuN, and their number was quantified. (O-R) Effects of Wnt/β-catenin signaling blockade by IWP-2 or ICG-001 on the enhanced stress resilience effects of GAS (n = 8). Data are mean ± standard error of the mean (SEM). * p < 0.05, ** p < 0.01, *** p < 0.001 vs Control group, # p < 0.05, ### p < 0.001 vs Stress group, & p < 0.05, && p < 0.01, && p < 0.01 vs GAS group (one-way ANOVA with Tukey's multiple-comparisons test). Details of the statistical analyses are provided in Supplementary Table 7. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Figure Legend Snippet: Blockade of Wnt/β-catenin signaling partially abolished the improvement effects of gastrodin on neurogenesis and behaviors in stress-exposed mice (A-B) Scheme for blocking the Wnt/β-catenin signaling with IWP-2 or ICG-001 in stress-exposed mice in present of Gastrodin (GAS) treatment. SPT, sucrose preference test; OFT, open field test; EPMT, elevated plus maze test; FST, forced swimming test. (C – H) Effects of Wnt/β-catenin signaling blockade by IWP-2 or ICG-001 by IWP-2 or ICG-001 on levels of Wnt, β-catenin, p -β-catenin, C-Myc and Cyclin D1 in hippocampus of GAS + Stress mice. (I-N) Effects of Wnt/β-catenin signaling blockade by IWP-2 or ICG-001 on the hippocampal neurogenesis of stress-exposed mice treated with GAS. Proliferating NSPCs were labeled with BrdU (green), and immature neurons were labeled with Doublecortin (DCX), mature neurons were labeled with NeuN, the newborn immature neurons differentiated from NSPCs were labeled both with DCX and BrdU, the newborn mature neurons were labeled with both BrdU and NeuN, and their number was quantified. (O-R) Effects of Wnt/β-catenin signaling blockade by IWP-2 or ICG-001 on the enhanced stress resilience effects of GAS (n = 8). Data are mean ± standard error of the mean (SEM). * p < 0.05, ** p < 0.01, *** p < 0.001 vs Control group, # p < 0.05, ### p < 0.001 vs Stress group, & p < 0.05, && p < 0.01, && p < 0.01 vs GAS group (one-way ANOVA with Tukey's multiple-comparisons test). Details of the statistical analyses are provided in Supplementary Table 7. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Techniques Used: Blocking Assay, Labeling, Control



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    Blockade of Wnt/β-catenin signaling partially abolished the improvement effects of gastrodin on neurogenesis and behaviors in stress-exposed mice (A-B) Scheme for blocking the Wnt/β-catenin signaling with IWP-2 or ICG-001 in stress-exposed mice in present of Gastrodin (GAS) treatment. SPT, sucrose preference test; OFT, open field test; EPMT, elevated plus maze test; FST, forced swimming test. (C – H) Effects of Wnt/β-catenin signaling blockade by IWP-2 or ICG-001 by IWP-2 or ICG-001 on levels of Wnt, β-catenin, p -β-catenin, C-Myc and Cyclin D1 in hippocampus of GAS + Stress mice. (I-N) Effects of Wnt/β-catenin signaling blockade by IWP-2 or ICG-001 on the hippocampal neurogenesis of stress-exposed mice treated with GAS. Proliferating NSPCs were labeled with BrdU (green), and immature neurons were labeled with Doublecortin (DCX), mature neurons were labeled with NeuN, the newborn immature neurons differentiated from NSPCs were labeled both with DCX and BrdU, the newborn mature neurons were labeled with both BrdU and NeuN, and their number was quantified. (O-R) Effects of Wnt/β-catenin signaling blockade by IWP-2 or ICG-001 on the enhanced stress resilience effects of GAS (n = 8). Data are mean ± standard error of the mean (SEM). * p < 0.05, ** p < 0.01, *** p < 0.001 vs Control group, # p < 0.05, ### p < 0.001 vs Stress group, & p < 0.05, && p < 0.01, && p < 0.01 vs GAS group (one-way ANOVA with Tukey's multiple-comparisons test). Details of the statistical analyses are provided in Supplementary Table 7. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Journal: Journal of Advanced Research

    Article Title: Gastrodin enhances stress resilience through promoting Wnt/β-Catenin-dependent neurogenesis

    doi: 10.1016/j.jare.2025.04.017

    Figure Lengend Snippet: Blockade of Wnt/β-catenin signaling partially abolished the improvement effects of gastrodin on neurogenesis and behaviors in stress-exposed mice (A-B) Scheme for blocking the Wnt/β-catenin signaling with IWP-2 or ICG-001 in stress-exposed mice in present of Gastrodin (GAS) treatment. SPT, sucrose preference test; OFT, open field test; EPMT, elevated plus maze test; FST, forced swimming test. (C – H) Effects of Wnt/β-catenin signaling blockade by IWP-2 or ICG-001 by IWP-2 or ICG-001 on levels of Wnt, β-catenin, p -β-catenin, C-Myc and Cyclin D1 in hippocampus of GAS + Stress mice. (I-N) Effects of Wnt/β-catenin signaling blockade by IWP-2 or ICG-001 on the hippocampal neurogenesis of stress-exposed mice treated with GAS. Proliferating NSPCs were labeled with BrdU (green), and immature neurons were labeled with Doublecortin (DCX), mature neurons were labeled with NeuN, the newborn immature neurons differentiated from NSPCs were labeled both with DCX and BrdU, the newborn mature neurons were labeled with both BrdU and NeuN, and their number was quantified. (O-R) Effects of Wnt/β-catenin signaling blockade by IWP-2 or ICG-001 on the enhanced stress resilience effects of GAS (n = 8). Data are mean ± standard error of the mean (SEM). * p < 0.05, ** p < 0.01, *** p < 0.001 vs Control group, # p < 0.05, ### p < 0.001 vs Stress group, & p < 0.05, && p < 0.01, && p < 0.01 vs GAS group (one-way ANOVA with Tukey's multiple-comparisons test). Details of the statistical analyses are provided in Supplementary Table 7. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Article Snippet: To study how Wnt/β-catenin signaling promotes neurogenesis and enhances stress resilience, we utilized IWP-2 (HY-13912, MCE), an inhibitor of Wnt, and ICG-001 (HY-14428, MCE), a β-catenin inhibitor.

    Techniques: Blocking Assay, Labeling, Control