ivm single in vivo micromanipulator  (Scientifica)

Journal: Frontiers in Veterinary Science

Article Title: Virtual screening, molecular dynamics simulations, and in vitro analysis of Sophora flavescens -derived aloperine against Haemonchus contortus

doi: 10.3389/fvets.2025.1620324

Figure Lengend Snippet: Inhibitory effect of ALO alone or in combination with IVM on the larval development of sensitive strain (A) and resistant strain (B) of Haemonchus contortus . ALO, aloperine.

Article Snippet: The mixture was incubated at 27°C in a biochemical incubator for 24 h. Subsequently, 80 μL of ALO (Baoji Fangsheng Biological Development Co., Ltd., Baoji, China), 80 μL of IVM (Shanghai Yuanye Bio-Technology Co., Ltd., Shanghai, China), a combination of 40 μL ALO + 40 μL IVM, and 1% DMSO (a negative control) was added to the system in individual wells.

Techniques:

Journal: Frontiers in Veterinary Science

Article Title: Virtual screening, molecular dynamics simulations, and in vitro analysis of Sophora flavescens -derived aloperine against Haemonchus contortus

doi: 10.3389/fvets.2025.1620324

Figure Lengend Snippet: Inhibitory effect of ALO alone or in combination with IVM on the migration of the sensitive (A) and resistant (B) strains of Haemonchus contortus . ALO, aloperine.

Article Snippet: The mixture was incubated at 27°C in a biochemical incubator for 24 h. Subsequently, 80 μL of ALO (Baoji Fangsheng Biological Development Co., Ltd., Baoji, China), 80 μL of IVM (Shanghai Yuanye Bio-Technology Co., Ltd., Shanghai, China), a combination of 40 μL ALO + 40 μL IVM, and 1% DMSO (a negative control) was added to the system in individual wells.

Techniques: Migration

Journal: Frontiers in Veterinary Science

Article Title: Virtual screening, molecular dynamics simulations, and in vitro analysis of Sophora flavescens -derived aloperine against Haemonchus contortus

doi: 10.3389/fvets.2025.1620324

Figure Lengend Snippet: Effect of ALO alone or in combination with IVM on the morphology of Haemonchus contortus . (A1) HC-S control group, (B1) HC-S IVM group, (C1) HC-S ALO group, (D1) HC-S ALO + IVM group, (A2) HC-R control group, (B2) HC-R IVM group, (C2) HC-R ALO group, and (D2) HC-R ALO + IVM group. ALO, aloperine.

Article Snippet: The mixture was incubated at 27°C in a biochemical incubator for 24 h. Subsequently, 80 μL of ALO (Baoji Fangsheng Biological Development Co., Ltd., Baoji, China), 80 μL of IVM (Shanghai Yuanye Bio-Technology Co., Ltd., Shanghai, China), a combination of 40 μL ALO + 40 μL IVM, and 1% DMSO (a negative control) was added to the system in individual wells.

Techniques: Control

Journal: Frontiers in Veterinary Science

Article Title: Virtual screening, molecular dynamics simulations, and in vitro analysis of Sophora flavescens -derived aloperine against Haemonchus contortus

doi: 10.3389/fvets.2025.1620324

Figure Lengend Snippet: Effect of ALO on rhodamine 123 accumulation. Rhodamine 123 (R, 1.5 μM) was treated with Rhodamine 123 (R, 1.5 μM) alone or in combination with DMSO (1%), picloram, IVM, or AIO + IVM for 24 h. Data are expressed as mean ± SEM ( n = 3). Statistical significance compared to the R-alone group was determined by one-way ANOVA with Tukey’s post hoc test (* p < 0.05, **** p < 0.0001).

Article Snippet: The mixture was incubated at 27°C in a biochemical incubator for 24 h. Subsequently, 80 μL of ALO (Baoji Fangsheng Biological Development Co., Ltd., Baoji, China), 80 μL of IVM (Shanghai Yuanye Bio-Technology Co., Ltd., Shanghai, China), a combination of 40 μL ALO + 40 μL IVM, and 1% DMSO (a negative control) was added to the system in individual wells.

Techniques: