inhba  (Proteintech)

Journal: Frontiers in Endocrinology

Article Title: Amelioration of polycystic ovarian morphology by Tokishakuyakusan in a PCOS rat model: association with bone morphogenetic protein 4

doi: 10.3389/fendo.2025.1649124

Figure Lengend Snippet: Bmp4 , Bmp5 , and Inhba expression in the ovaries. (A–C) Relative mRNA expression quantified by real-time PCR. Expression levels are relative to 18S ribosomal RNA. Data are shown as mean ± standard deviation with individual data points. P -values were obtained by one-way analysis of variance (ANOVA) with Tukey’s post hoc test (vehicle: n = 6; DHT: n = 9; DHT+TSS: n = 10). (D–G) Correlation analysis between gene expression and ovarian follicle counts in the DHT and DHT+TSS groups ( n = 6 per group). The correlation coefficient ( r 2 ) and P -value were calculated. DHT, 5α-dihydrotestosterone; TSS, Tokishakuyakusan; Bmp, bone morphogenetic protein; Inhba, inhibin-βa; PCR, polymerase chain reaction.

Article Snippet: The following TaqMan primer–probe sets (Thermo Fisher Scientific) were used: Bmp4 , Rn00432087_m1; Bmp5 , Rn01447676_m1; inhibin-βa ( Inhba ), Rn01538592_m1; steroidogenic acute regulatory protein ( Star ), Rn00580695_m1; cytochrome P450 11A1 ( Cyp11a1 ), Rn00568733_m1; 3β hydroxysteroid dehydrogenase ( Hsd3b ), Rn01789220_m1; 18s , Hs99999901_s1; and Gapdh , Rn01775763_g1.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Standard Deviation, Gene Expression, Polymerase Chain Reaction

Journal: Frontiers in Endocrinology

Article Title: Amelioration of polycystic ovarian morphology by Tokishakuyakusan in a PCOS rat model: association with bone morphogenetic protein 4

doi: 10.3389/fendo.2025.1649124

Figure Lengend Snippet: Effect of Tokishakuyakusan (TSS) on target gene expressions in granulosa cells (GCs) derived from prenatally 5α-dihydrotestosterone (DHT)-treated rats. (A) Timeline of ovarian GC collection. (B, C) Relative mRNA expression of Bmp4 and Inhba in primary cultured GCs after 24 h of TSS treatment (125–500 μg/mL). Expression levels are relative to Gapdh . Data are shown as mean ± standard deviation with individual data points ( n = 3 per group). ** P < 0.01, P -values for the untreated control were obtained using one-way analysis of variance (ANOVA) followed by Dunnett’s post hoc test. PMSG, pregnant mare serum gonadotropin; Bmp, bone morphogenetic protein; Inhba, inhibin-βa; Gapdh, glyceraldehyde-3-phosphate dehydrogenase.

Article Snippet: The following TaqMan primer–probe sets (Thermo Fisher Scientific) were used: Bmp4 , Rn00432087_m1; Bmp5 , Rn01447676_m1; inhibin-βa ( Inhba ), Rn01538592_m1; steroidogenic acute regulatory protein ( Star ), Rn00580695_m1; cytochrome P450 11A1 ( Cyp11a1 ), Rn00568733_m1; 3β hydroxysteroid dehydrogenase ( Hsd3b ), Rn01789220_m1; 18s , Hs99999901_s1; and Gapdh , Rn01775763_g1.

Techniques: Derivative Assay, Expressing, Cell Culture, Standard Deviation, Control

Journal: Oncology Research

Article Title: INHBA Promotes the Progression of Gastric Cancer by Activating MAPK Signaling Pathway via Targeting ITGA6

doi: 10.32604/or.2025.070333

Figure Lengend Snippet: INHBA promotes the growth of GC in vivo . ( A ) Verification of stable bead over-expression efficiency was detected by qRT-PCR. ( B ) Verification of stable bead over-expression efficiency was detected by WB. ( C ) subcutaneous xenograft models. ( D ) Tumor growth curve. ( E , F ) Images ( E ) and weight ( F ) of subcutaneous xenograft models were analyzed. ( G ) Representative images of tumor tissue sections stained with HE. (magnification ×400). ( H ) Representative image of Ki67 IHC staining in tumor tissue sections. (magnification ×400). Data are shown as means ± SD.* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: The following primary antibodies obtained from Proteintech (Wuhan, China) were used: INHBA (diluted 1:200; Proteintech; Catalog number: 17524-1-AP), Ki67 (diluted 1:100; Proteintech; Catalog number: 27309-1-AP), and ITGA6 (diluted 1:500; Proteintech; Catalog number: 27189-1-AP).

Techniques: In Vivo, Over Expression, Quantitative RT-PCR, Staining, Immunohistochemistry

Journal: Oncology Research

Article Title: INHBA Promotes the Progression of Gastric Cancer by Activating MAPK Signaling Pathway via Targeting ITGA6

doi: 10.32604/or.2025.070333

Figure Lengend Snippet: ITGA6 is the regulatory target of INHBA. ( A ) Differential expression genes (DEGs) heat map identified by RNA-seq. ( B ) The number of DEGs identified by RNA-seq. ( C ) Overlap analysis of RNA-seq and STAD-DEGs-up identified genes. ( D ) Expression of ITGA6 in GC paired tissue cohort from the GEPIA2 database. ( E ) Subcellular localization of ITGA6 from GeneCards. ( F , G ) The interaction between INHBA and ITGA6 was verified by Co-IF ( F ) and Co-IP ( G ) experiments. Scale bar, 50 μm. ( H ) qRT-PCR was used to detect ITGA6 mRNA expression after INHBA knockdown in NUGC-3 cells. ( I ) WB was used to detect ITGA6 protein expression after INHBA knockdown in NUGC-3 cells. ( J ) qRT-PCR was used to detect ITGA6 mRNA expression after INHBA overexpression in AGS cells. ( K ) WB was used to detect ITGA6 protein expression after INHBA overexpression in AGS cells. Data are presented as means ± SD. * p < 0.05, ** p < 0.01, **** p < 0.0001

Article Snippet: Primary INHBA antibody (diluted 1:50; Proteintech; Catalog number: 17524-1-AP) and ITGA6 antibody (diluted 1:50; Santa Cruz Biotechnology, Dallas, TX, USA; Catalog number: sc-374057) were added to the cells and incubated overnight at 4°C.

Techniques: Quantitative Proteomics, RNA Sequencing, Expressing, Co-Immunoprecipitation Assay, Quantitative RT-PCR, Knockdown, Over Expression

Journal: Oncology Research

Article Title: INHBA Promotes the Progression of Gastric Cancer by Activating MAPK Signaling Pathway via Targeting ITGA6

doi: 10.32604/or.2025.070333

Figure Lengend Snippet: Expression of ITGA6 and its interaction with INHBA in GC. ( A ) The basal expression of ITGA6 mRNA in GC cell lines and GES-1 was detected by qRT-PCR. ( B ) The basal expression of ITGA6 protein in cell lines was detected by WB. ( C ) The expression of ITGA6 protein in 30 pairs of GC paired tissues was detected by IHC. (magnification ×400). The red arrow indicates the ITGA6 expression area, which appears brown or yellow. ( D,E ) Four siRNA-ITGA6 sequences were transfected simultaneously into AGS cells. ( F,G ) The relative expression level of INHBA mRNA and protein was detected by qRT-PCR and WB after instantaneous co-transfection of INHBA siRNA and ITGA6 overexpression plasmid in NUGC-3. ( H,I ) The relative expression level of INHBA mRNA and protein were detected by qRT-PCR and WB after instantaneous co-transfection of ITGA6 siRNA and INHBA overexpression plasmid in AGS. Data are presented as means ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: Primary INHBA antibody (diluted 1:50; Proteintech; Catalog number: 17524-1-AP) and ITGA6 antibody (diluted 1:50; Santa Cruz Biotechnology, Dallas, TX, USA; Catalog number: sc-374057) were added to the cells and incubated overnight at 4°C.

Techniques: Expressing, Quantitative RT-PCR, Transfection, Cotransfection, Over Expression, Plasmid Preparation

Journal: Oncology Research

Article Title: INHBA Promotes the Progression of Gastric Cancer by Activating MAPK Signaling Pathway via Targeting ITGA6

doi: 10.32604/or.2025.070333

Figure Lengend Snippet: Oncogenic function of INHBA depends on ITGA6 and the MAPK signaling pathway. ( A ) KEGG enrichment result of the differentially expressed gene sets from the GEPIA2 database. ( B ) The expression levels of MAPK signaling pathway-related proteins were detected by WB after instantaneous co-transfection of INHBA siRNA and ITGA6 overexpression plasmid in NUGC-3. ( C,E,G,I,J ) The effects of ITGA6 on INHBA in NUGC-3 cells were detected by CCK-8 assay ( C ), colony formation assay ( E ), wound healing assay ( G ), Scale bar, 200 μm, Transwell migration assay ( I ) and Transwell invasion assay ( J ). Scale bar, 100 μm. ( D,F,H,K,L ) The effects of ITGA6 on INHBA in AGS cells were detected by CCK-8 assay ( D ), colony formation assay ( F ), wound healing assay ( H ), Scale bar, 200 μm, Transwell migration assay ( K ) and Transwell invasion assay ( L ). Scale bar, 100 μm. Data are presented as means ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: Primary INHBA antibody (diluted 1:50; Proteintech; Catalog number: 17524-1-AP) and ITGA6 antibody (diluted 1:50; Santa Cruz Biotechnology, Dallas, TX, USA; Catalog number: sc-374057) were added to the cells and incubated overnight at 4°C.

Techniques: Expressing, Cotransfection, Over Expression, Plasmid Preparation, CCK-8 Assay, Colony Assay, Wound Healing Assay, Transwell Migration Assay, Transwell Invasion Assay