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Molecular Dynamics Inc imagequant data analysis software
Effect of CADO and PGE2 on cAMP production (a) and CREB (b) and Csk (c) phosphorylation. a TILs #2009 at stage II were incubated with CADO (50 μM) and/or PGE2 (0.5 μM) or CGS (50 μM) and/or butaprost (5 μM) for 10 min. The levels of cAMP in cellular extracts were analyzed using mass spectrometry. b, c TILs #2009 at stage II were stimulated with anti-CD3 in the presence of CADO (50 μM) and/or PGE2 (0.5 μM) for 30 min. TIL protein extracts (50 μg) were resolved using 10% SDS-PAGE and transferred to PVDF membranes. The level of CREB (b) and Csk (c) phosphorylation was analyzed using specific antibodies. The optic density (OD) ratio of the bands was analyzed using <t>ImageQuanT</t> data analysis software (Molecular Dynamics)
Imagequant Data Analysis Software, supplied by Molecular Dynamics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
imagequant data analysis software - by Bioz Stars, 2026-09
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1) Product Images from "Receptor desensitization and blockade of the suppressive effects of prostaglandin E 2 and adenosine on the cytotoxic activity of human melanoma-infiltrating T lymphocytes"

Article Title: Receptor desensitization and blockade of the suppressive effects of prostaglandin E 2 and adenosine on the cytotoxic activity of human melanoma-infiltrating T lymphocytes

Journal: Cancer immunology, immunotherapy : CII

doi: 10.1007/s00262-010-0924-z

Effect of CADO and PGE2 on cAMP production (a) and CREB (b) and Csk (c) phosphorylation. a TILs #2009 at stage II were incubated with CADO (50 μM) and/or PGE2 (0.5 μM) or CGS (50 μM) and/or butaprost (5 μM) for 10 min. The levels of cAMP in cellular extracts were analyzed using mass spectrometry. b, c TILs #2009 at stage II were stimulated with anti-CD3 in the presence of CADO (50 μM) and/or PGE2 (0.5 μM) for 30 min. TIL protein extracts (50 μg) were resolved using 10% SDS-PAGE and transferred to PVDF membranes. The level of CREB (b) and Csk (c) phosphorylation was analyzed using specific antibodies. The optic density (OD) ratio of the bands was analyzed using ImageQuanT data analysis software (Molecular Dynamics)
Figure Legend Snippet: Effect of CADO and PGE2 on cAMP production (a) and CREB (b) and Csk (c) phosphorylation. a TILs #2009 at stage II were incubated with CADO (50 μM) and/or PGE2 (0.5 μM) or CGS (50 μM) and/or butaprost (5 μM) for 10 min. The levels of cAMP in cellular extracts were analyzed using mass spectrometry. b, c TILs #2009 at stage II were stimulated with anti-CD3 in the presence of CADO (50 μM) and/or PGE2 (0.5 μM) for 30 min. TIL protein extracts (50 μg) were resolved using 10% SDS-PAGE and transferred to PVDF membranes. The level of CREB (b) and Csk (c) phosphorylation was analyzed using specific antibodies. The optic density (OD) ratio of the bands was analyzed using ImageQuanT data analysis software (Molecular Dynamics)

Techniques Used: Phospho-proteomics, Incubation, Mass Spectrometry, SDS Page, Software

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Article Title: Cooperation of adenosine and prostaglandin E2 (PGE2) in amplification of cAMP-PKA signaling and immunosuppression.
Article Snippet: Introduction We hypothesize that adenosine and PGE2 could have a complementary immunosuppressive eVect that is mediated via common cAMP-PKA signaling.. Materials and methods To test this hypothesis, the eVect of adenosine and PGE2 on the cytotoxic activity and cytokine production of lymphokine activated killer (LAK) cells was investigated.. Results PGE2 and adenosine inhibited LAK cells cytotoxic activity and production of INF, GM-CSF and TNF.

Article Title: Receptor desensitization and blockade of the suppressive effects of prostaglandin E 2 and adenosine on the cytotoxic activity of human melanoma-infiltrating T lymphocytes
Article Snippet: The signals were detected by ECL (Amersham). .. Films were scanned and analyzed by ImageQuanT data analysis software (Molecular Dynamics) [ 17 ]. ..

Article Title: Receptor desensitization and blockade of the suppressive effects of prostaglandin E 2 and adenosine on the cytotoxic activity of human melanoma-infiltrating T lymphocytes
Article Snippet: .. The optic density (OD) ratio of the bands was analyzed using ImageQuanT data analysis software (Molecular Dynamics) We next analyzed downstream mechanisms of cAMP signaling following treatment of stage II TILs with CADO or PGE 2 . ..

Article Title: Pegylated, steptavidin-conjugated quantum dots are effective detection elements for reverse-phase protein microarrays.
Article Snippet: FDA-NCI Clinical Proteomics Program, Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, Office of Cellular and Gene Therapy, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892, and Department of Pathology, Division of Translational Pathology, University of Texas Southwestern, Dallas, Texas 75390.. Received December 2, 2004; Revised Manuscript Received March 24, 2005



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Effect of CADO and PGE2 on cAMP production (a) and CREB (b) and Csk (c) phosphorylation. a TILs #2009 at stage II were incubated with CADO (50 μM) and/or PGE2 (0.5 μM) or CGS (50 μM) and/or butaprost (5 μM) for 10 min. The levels of cAMP in cellular extracts were analyzed using mass spectrometry. b, c TILs #2009 at stage II were stimulated with anti-CD3 in the presence of CADO (50 μM) and/or PGE2 (0.5 μM) for 30 min. TIL protein extracts (50 μg) were resolved using 10% SDS-PAGE and transferred to PVDF membranes. The level of CREB (b) and Csk (c) phosphorylation was analyzed using specific antibodies. The optic density (OD) ratio of the bands was analyzed using <t>ImageQuanT</t> data analysis software (Molecular Dynamics)
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Effect of CADO and PGE2 on cAMP production (a) and CREB (b) and Csk (c) phosphorylation. a TILs #2009 at stage II were incubated with CADO (50 μM) and/or PGE2 (0.5 μM) or CGS (50 μM) and/or butaprost (5 μM) for 10 min. The levels of cAMP in cellular extracts were analyzed using mass spectrometry. b, c TILs #2009 at stage II were stimulated with anti-CD3 in the presence of CADO (50 μM) and/or PGE2 (0.5 μM) for 30 min. TIL protein extracts (50 μg) were resolved using 10% SDS-PAGE and transferred to PVDF membranes. The level of CREB (b) and Csk (c) phosphorylation was analyzed using specific antibodies. The optic density (OD) ratio of the bands was analyzed using ImageQuanT data analysis software (Molecular Dynamics)

Journal: Cancer immunology, immunotherapy : CII

Article Title: Receptor desensitization and blockade of the suppressive effects of prostaglandin E 2 and adenosine on the cytotoxic activity of human melanoma-infiltrating T lymphocytes

doi: 10.1007/s00262-010-0924-z

Figure Lengend Snippet: Effect of CADO and PGE2 on cAMP production (a) and CREB (b) and Csk (c) phosphorylation. a TILs #2009 at stage II were incubated with CADO (50 μM) and/or PGE2 (0.5 μM) or CGS (50 μM) and/or butaprost (5 μM) for 10 min. The levels of cAMP in cellular extracts were analyzed using mass spectrometry. b, c TILs #2009 at stage II were stimulated with anti-CD3 in the presence of CADO (50 μM) and/or PGE2 (0.5 μM) for 30 min. TIL protein extracts (50 μg) were resolved using 10% SDS-PAGE and transferred to PVDF membranes. The level of CREB (b) and Csk (c) phosphorylation was analyzed using specific antibodies. The optic density (OD) ratio of the bands was analyzed using ImageQuanT data analysis software (Molecular Dynamics)

Article Snippet: Films were scanned and analyzed by ImageQuanT data analysis software (Molecular Dynamics) [ 17 ].

Techniques: Phospho-proteomics, Incubation, Mass Spectrometry, SDS Page, Software